LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Xiaoke Peng,Xiaojia Yan,David Julian McClements,Shuai Chen,Cuicui Ma,Xuebo Liu,Guoqiang Zhang,Fuguo Liu
The objective of this study was to prepare dendrobium officinale polysaccharide-epigallocatechin-3-gallate (DOP-EGCG) covalent conjugates and non-covalent complexes to enhance the antioxidant properties of the polysaccharide. The conjugates were synthesized via free radical induction, whereas the complexes were formed through physical mixing. The structure and properties of the conjugates and complexes were then characterized. C-H bonds and hydrogen bonds were found to play a crucial role in the formation of the conjugates. Conjugating DOP and EGCG decreased the crystallinity of the polysaccharide and altered the crystal morphology of the polyphenol. The thermal stability of DOP in the EGCG complexes (99.2 °C) was lower than that of free DOP (103.0 °C), whereas that of the DOP in the EGCG conjugates was higher (113.7 °C). The conjugation of EGCG led to an increase in its phenolic content (112.9 ± 1.8 g/kg), which enhanced its antioxidant properties. Indeed, the conjugates had higher antioxidant properties, as seen by an increase in free radical scavenging, reactive oxygen species quenching, and reducing ability, than the complexes. The present study demonstrated that the formation of polysaccharide-polyphenol conjugates could enhance the antioxidant properties of DOP, thereby expanding its range of application as a functional ingredient in foods and beverages.
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Advanced Science
·
2025
·
IF 14.1
Chenxi Xu,Xiaoling Huang,Zhichao Deng,Ruiying Wang,Seyedalireza Ghazimirsaeid,Li Yao,Yuanyuan Zhu,Bowen Gao,Junlong Fu,Mingxin Zhang,Mei Yang,Mingzhen Zhang
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder driven by genetic susceptibility, immune dysregulation, and intestinal barrier dysfunction. Current therapies primarily target immune suppression but show limited efficacy in barrier repair. Here, carbon dots derived from the Chinese herbal medicine Dendrobium officinale (DO-CDs) , which exhibit antioxidant enzyme activity, are synthesized using a hydrothermal method. These DO-CDs, characterized by an abundance of surface functional groups, are demonstrated to scavenge ROS, suppress M1 macrophage polarization, as well as downregulate pro-inflammatory cytokine expression. In both acute and chronic colitis models, DO-CDs demonstrate multimodal barrier-repair properties. It is shown that DO-CDs can notably restore colon length, reduce the infiltration of inflammatory cells, and enhance both the quantity of goblet cells and the expression of mucins. Furthermore, the expression of intestinal epithelial tight junction proteins is significantly upregulated following DO-CDs treatment, thereby effectively strengthening the intestinal epithelial barrier function. Importantly, DO-CDs modulate the Th1/Treg ratio by downregulating the proportions of dendritic cells and M1 macrophages, thus reestablishing intestinal immune homeostasis. These coordinated actions on the mucus-epithelium-immune triad demonstrate the unique capacity of DO-CDs for holistic barrier reconstruction. The work provides a mechanistic foundation for herbal precursor-derived carbon dots as multi-target therapeutics in IBD.
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高影响因子
Foods
·
2022
·Vol. 12
·期 1
·150
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IF 5.561
Hang Chen,Xueqin Shi,Lanyan Cen,Lin Zhang,Yifeng Dai,Shuyi Qiu,Xiangyong Zeng,Chaoyang Wei
Fermentation is an effective method for enhancing the biological activity of polysaccharides, but research on its effect onDendrobium officinalpolysaccharides is rare. In this study, the effects of mono-fermentation (Saccharomyces cerevisiaeFBKL2.8022,Sc;Wickerhamomyces anomalousFBKL2.8023,Wa) and co-fermentation (Sc+Wa) on the physicochemical properties and bioactivity ofDendrobium officinalpolysaccharides were investigated. Meanwhile, the polysaccharide (DOP) obtained fromDendrobium officinalewas used as a control. Four homogeneous polysaccharides were obtained by isolation and purification and named DOSCP, DOWAP, DOSWP, and DOP. The results showed that DOSCP, DOWAP, DOSWP, and DOP consisted of mannose and glucose with ratios of 3.31:1, 5.56:1, 2.40:1, and 3.29:1, respectively. The molecular weights (Mws) of the four polysaccharides were 25.73 kDa, 15.01 kDa, 17.67 kDa, and 1268.21 kDa. The antioxidant activity of DOSCP, DOWAP, and DOSWP was better than that of DOP. Additionally, all four polysaccharides were able to reduce the inflammatory response of LPS-induced RAW 264.7 macrophages in the mice without a significant difference. Yeast fermentation significantly reduced the molecular weight and improved the antioxidant activity ofDendrobium officinalepolysaccharides, indicating a potential way to improve its antioxidant activity.Keywords:Dendrobium officinale;polysaccharides;fermentation;antioxidant activity;immune activity
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Food Bioscience
·
2023
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IF 5.2
Xuewen Dong,Jie Tang,Xiaoting Qin,Shenglan Tian,Huasi Lin,Qiang Yu,Xiaobo Hu,Mingyong Xie
The research was conducted to investigate the quality changes of basic physicochemical indicators, functional components and appearance, such as, pH, total color difference, Browning Index, polyphenols, flavonoids , anthocyanins , VC, and 5-hydroxymethylfurfural content and so on of Dendrobium officinale flower juice (DOFJ) under the dark storage conditions of 4, 25 and 37 °C and the natural light irradiation storage conditions of 25 °C for 8 weeks. Correlation and path analyses were used to analyze the causes of color degradation of DOFJ. Although measures had been taken during the preparation process to inhibit enzymatic browning by deactivating the activity of polyphenol oxidase (PPO), DOFJ was still unstable under four storage conditions, especially for color degradation. With the increase of temperature and time, the basic physicochemical indexes did not change significantly, but the content of functional components gradually decreased, and the appearance gradually deteriorated. The path analysis indicated that anthocyanins degradation was the key factor of color degradation of DOFJ, and low temperature and light protection could delay the degradation of anthocyanins. The main cause of color degradation of DOFJ during storage was identified, which provided a basis for further inhibiting color degradation of flower juice.
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ACS Applied Polymer Materials
·
2024
·
IF 4.4
Yuhan Wang,Shuai Liu,Jin Jia,Zijun Guan,Yi Dong,Haibo Wang
Owing to their superior flexibility and compatibility with biological systems, dual-network hydrogels have garnered significant interest in the realm of research dedicated to the development of flexible wearable sensors. Electronic skin, or E-skin, has the capability to seamlessly combine high mechanical resilience, sensory acuity, and a broad spectrum of adhesion. The potential of this technology for applications in the fields of intelligent robotics and prosthetic devices has garnered considerable attention. In this study, a polymer substance, dendrobium officinale polysaccharide (DOP), was successfully introduced as the basic skeleton of the second-layer network structure of the hydrogel, and as a result, a multifunctional conductive hydrogel (PASD-Zn hydrogels) was synthesized. Notably, the addition of DOP to the hydrogel formulation resulted in a well-balanced set of mechanical properties (with an elongation at break of 1248% and a tensile strength of 0.238 MPa), excellent plasticity capabilities, and significant self-recovery. As a wireless strain sensor, the PASD-Zn sensor can monitor mechanically conducted signals associated with human motion. Notably, the hydrogel exhibits excellent self-adhesion on a variety of surfaces. This hydrogel holds great promise for advancing wearable devices and bionic skin.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Xuemei Lu,Ke Hu,Minggui Ou,Min Li,Xinchun Zhang,Xingyu Zhan,Xun Liao,Ming Li,Rongyu Li
The soft rot disease caused by Fusarium oxysporum leads to a significant reduction in the yield of Dendrobium officinale . However, research into the synergistic inhibitory effect of essential oils in D. officinale is extremely limited. In this study, we systematically investigated the direct and indirect inhibitory activity of carvacrol, citral and their combination against F. oxysporum , and their synergistic inhibitory mechanism. Carvacrol and citral exhibited significant direct and indirect inhibitory activity against F. oxysporum with EC 50 values of 54.37 mg/L and 119.32 mg/L (direct), 18.01 μL/(L•air) and 48.70 μL/(L•air) (indirect). Synergistic analysis revealed that the optimal synergistic toxicity of carvacrol and citral combination (Ca•Ci) against F. oxysporum was 10:1, with co-toxicity coefficient (CTC) of 131.57 and EC 50 value of 44.24 mg/L. Microscopy confirmed that the Ca•Ci led to more significant tip constriction, uneven surfaces and serious rupture of F. oxysporum mycelia than single compound. Transmission electron microscopy (TEM) showed that Ca•Ci also caused significant ultrastructural alterations to F. oxysporum , manifesting as cytoplasmic disorganization and partial organellar disintegration. Moreover, Ca•Ci dramatically increased the sensitivity of F. oxysporum to calcofluor white compared with a single compound. Ca•Ci also considerably upregulated the expression of chitinase-related gene ( FOXG _12882) and β -1,3-glucanase-related gene ( FOXG _10637) in F. oxysporum , resulting in higher chitinase and β -1,3-glucanase activity. However, it should be noted that carvacrol exerted a greater contribution than citral. In conclusion, the combination of carvacrol and citral greatly disrupted the cell wall integrity of F. oxysporum , thereby exhibiting a synergistic effect.
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CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Shao-Jun Zheng,Xiao-Han Diao,Xiao-Yan Gao,Nai-Dong Chen
Dendrobium officinale polysaccharides (DOP) exhibit anticancer potential against colorectal cancer (CRC), yet their mechanisms remain unclear. This study investigated DOP's effects on HCT-116 cells. DOP dose- and time-dependently inhibited proliferation and induced apoptosis, evidenced by elevated cleaved caspase-3/-9, poly ADP-ribose polymerase (PARP), Bax, and reduced Bcl-2. Concurrently, DOP activated autophagy (increased LC3-II/I, Beclin-1; decreased p62) via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) suppression. Combining DOP with the autophagy inhibitor chloroquine enhanced apoptosis (higher cleaved PARP, Bax; lower Bcl-2) and reactivated PI3K/Akt/mTOR signaling. These findings demonstrate DOP inhibits CRC by dual induction of apoptosis and autophagy blockade through PI3K/Akt/mTOR inhibition, with autophagy suppression synergistically augmenting apoptosis. The study highlights DOP's translational potential as a functional food or adjuvant therapy, proposing a novel combinatorial strategy for CRC treatment. Graphical Dendrobium officinale polysaccharides (DOP) inhibit HCT-116 colorectal cancer cell proliferation and induce apoptosis by upregulating cleaved caspase-3/-9, PARP, and Bax while downregulating Bcl-2. DOP activates autophagy via PI3K/Akt/mTOR inhibition. Combining DOP with chloroquine enhances apoptosis, revealing autophagy blockade as a synergistic anticancer mechanism.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Wujin Yu,Peng Chen,Xinhui Xu,Lanlan Nie,Zhenyu Huang,Ahsan Hafiz Muhammad,Yan Wang,Tingting Bu,Peilong Sun,Ming Cai
Dendrobium officinale has garnered significant attention due to its notable bioactivity and health benefits. The polysaccharide extracted from Dendrobium officinale (DOP) is the primary source of its functionality. However, its poor solubility and high viscosity necessitate the modification of its physicochemical properties. This study aimed to investigate the impact of cold plasma treatment on the physicochemical and functional properties of DOP. The results indicated that cold plasma treatment substantially increased the solubility of DOP from 87.5 % to 96.30 % and enhanced its hydrophilicity with a lower water contact angle, primarily attributed to the etching effect of plasma. Furthermore, the study confirmed the co-occurrence of cross-linking and depolymerization during the plasma discharge process. The plasma treatment reduced the viscosity of the polysaccharide solution by 10 folds and increased its thermal stability. The microstructure of the polysaccharide, examined by SEM and AFM, revealed that plasma treatment disrupted the cross-linking structure, leading to smooth agglomeration at high intensity. Moreover, the antioxidant capacity and immunoreactivity of plasma-treated DOP have been improved, and the free radical scavenging rate of the plasma-treated could reach 34.40 % at a concentration of 1 mg/mL, stimulating the secretion of cytokines TNF-α (increased 20.70 %), IL-1β (increased 26.94 %), IL-6 (increased 25.32 %), and IL-10 (increased 13.03 %) in in vitro cell tests. Accordingly, cold plasma treatment could be a novel approach to modifying polysaccharides and improving their potential applications in food and pharmaceutical sectors.
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Food Bioscience
·
2026
·
IF 6.2
Jiajing Yang,Yan Long,Kun Lei,Haichun Hu,Yujie Chen,Wenjie Ding,Haiyu Ji,Juan Yu
Selenium is essential for maintaining immune homeostasis, yet its inorganic form is characterized by low bioavailability and potential toxicity. A naturally acetylated Dendrobium officinale polysaccharide (DOP) with prominent immunomodulatory activities, but its was isolated in our previous study, whereas its immunological efficacy can be further improved throughvia structural modification. Selenium-modified D. officinale polysaccharides (SeDOP) were synthesized via the HNO 3 -Na 2 SeO 3 method in this study, with a selenium content of 0.076 ± 0.015%, and the preparation yield of SeDOP reached 62.5 ± 3.48% based on the initial DOP mass. The immunoregulatory function of SeDOP was further evaluated using a cyclophosphamide (CTX)-induced mouse model. Results demonstrated that the compositions of gut microbiota were positively regulated by SeDOP, as evidenced by the significant enrichment of beneficial bacterial genera including Lactobacillus , Limosilactobacillus , and Truepera . Metabolomic analysis revealed that SeDOP specifically activated indole alkaloid biosynthesis pathways, which were correlated with restored CD19 + B cell proportions in mice. Collectively, SeDOP attenuates CTX-induced immune injury by remodeling gut microbiota compositions and modulating indole alkaloid metabolism, and promotes the regeneration of lymphocytes, highlighting its potential as a dual-functional selenium-modified polysaccharide for immunoprotection and gut health.
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Food Chemistry-X
·
2022
·
IF 6.443
Hualing Xie,Jingyu Fang,Mohamed A. Farag,Zhenhao Li,Peilong Sun,Ping Shao
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF- α , TGF- β 1, IL-6, IL-1 β ) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides , Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.9
Hui Wang,Rui He,Kuan Chang,Jing Xie,Jinhua Li,Guangli Wang,Xiaodong Yan,Jing Wang
Mannose erythritol lipid (MEL-B) lamellar vesicles are promising biocompatible carriers for skincare, but their tendency to form unstable, micron-sized vesicles when used alone limits their application. Herein, we introduce the Gemini surfactant sodium dilauramidoglutamide lysine (DLGL) to overcome this limitation. Highly stable MEL-B-based unilamellar nanovesicles (< 100 nm) are formulated with DLGL. At an optimal DLGL/MEL-B ratio of 0.2, the binary vesicles exhibit excellent stability, showing negligible size variation (< 3 nm) across different storage temperatures and maintaining a zeta potential absolute value consistently above 30 mV. These vesicles significantly enhance the skin permeation of hydrophilic Dendrobium officinale oligosaccharides (DOOS), achieving twice the cumulative permeation compared to the pure DOOS solution at 8 h. Moreover, the formation of ternary vesicles by incorporating lecithin further enhance transdermal delivery.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Feixiong Zheng,Zhangting Xu,Xiaoji Deng,Yiming Sun,Jaime A. Teixeira da Silva,Xiaoxia Shen,Zhenming Yu
Salt stress severely constrains plant growth and productivity, so understanding the underlying mechanisms of salt stress is crucial for the genetic improvement of plants. Aquaporins (AQPs), which facilitate the transport of water or nutrients, exert an indispensable role in plants' response to salt stress. The AQP family in Dendrobium officinale , a commercially important medicinal and edible orchid, has not been identified or functionally characterized, while an understanding of this plant's tolerance to salinity remains unexplored. In this study, a genome-wide identification of AQP genes in D. officinale was performed using a salt-induced transcriptome. The 28 AQP members were mined and classified into four subfamilies (NIPs, PIPs, SIPs, and TIPs), and their physicochemical and bioinformatic properties were analyzed. Overexpression of DoTIP1-1 in D. officinale significantly enhanced salt tolerance via the accumulation of proline and water-soluble polysaccharides that improved water retention. Furthermore, salt tolerance was fortified by the improved activity of antioxidant enzymes (peroxidase and superoxide dismutase), allowing reactive oxygen species to be scavenged, and augmenting photosynthetic efficiency due to the upregulated expression of stress-responsive genes. This study provides the first comprehensive analysis of the AQP family in D. officinale and demonstrates that DoTIP1-1 is a key genetic determinant for enhancing salt tolerance, offering significant potential for the genetic improvement of this plant to resist abiotic stress.
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