ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Hongli Zheng,Qingrong Zhang,Zhensheng Hong,Yanyu Lin,Hong Dai
Prostaglandin E1, a cardiac biomarker, is a pivotal member of the prostaglandin family which exerts diverse and powerful effects on cell function in many organ systems. In this study, a new approach for the ultrasensitive determination of prostaglandin E1 was proposed by using the ECL of peroxydisulfate with oxygen as coreactant. Compared to most peroxydisulfate ECL sensor, this proposed ECL strategy propounded employing oxygen evolution reaction to enhance the concentration of oxygen, which was unprecedented in ECL sensing platform. Herein, ultrathin NiCo 2 O 4 nanosheets with large surface area and mesoporous structure were prepared by a facile method, and adamantane as enhancer was decorated on the surface of amino-modified NiCo 2 O 4 to generate a bifunctional catalyst of NiCo 2 O 4 @AD(Prostaglandin E), which possessed excellent catalytic performance toward the oxygen evolution reaction. Accordingly, the sensitivity of ECL immunosensor was enhanced with the increasing concentration of oxygen by the bifunctional catalyst of NiCo 2 O 4 @AD. And the ECL response of the peroxydisulfate would decrease owing to the interaction between antibody and antigen on the modified electrode . Consequently, on the basis of the ECL intensity change of peroxydisulfate, the proposed immunosensor has realized ultrasensitive PGE1 assay with a wide linear detection range of 0.1 fg/ml to1 ng/ml and a detection limit of 0.1 fg/ml. The proposed ECL immunosensor was performed to analyze prostaglandin E1 in human serum, and satisfactory recoveries were obtained, indicating its potential applications in clinical diagnostics.
研究领域:
分析化学
·
生物化学
·
材料科学
·
医学诊断
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产品关联
DOI
高影响因子
ANALYST
·
2024
·Vol. 149
·期 4
·1318-1326
·
IF 3.6
Kaida Kuang,Ya Li,Yang Chen,Yu Ji,Nengqin Jia
Conventional single-signal or emerging sandwich-type double-signal electrochemiluminescence (ECL) immunosensors/aptasensors have offered accurate detection of small molecules, yet suffer from complicated setup, long processing time, and non-reusability. Here, we demonstrate a simplified molecularly imprinted ECL sensor based on Mn2SnO4 nanocubes. As an n-type semiconductor, Mn2SnO4 has numerous active sites that can capture electrons to accelerate chemical reactions, resulting in enhanced ECL activity and stability. For the first time, we verify a robust cathodic ECL emission of Mn2SnO4 luminophores in the presence of K2S2O8 coreactants. The proposed ECL sensor applies to the sensitive detection of ribavirin (RBV), endowing a wide linear range (1–2000 ng mL−1), low detection limit (0.85 ng mL−1, S/N = 3), high stability, specificity, and reproducibility, and the detection capability in real milk and chicken samples. This work highlights single semiconductor luminophore-driven molecularly imprinted ECL sensors, meeting the original aspiration of uncomplicated but high-performance sensing in food safety inspection.
研究领域:
分析化学
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生物医学工程
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食品安全
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 15
·3603-3609
·
IF 4.2
Ju-Zheng Wang,Yi-Xuan Li,Qiaoting Yang,Junji Li,Jérome Chauvin,Xue-Ji Zhang,Serge Cosnier,Robert S. Marks,Dan Shan
The emergence of novel pathogens, as well as their frequent variants, raises the significance of developing superior and versatile sensing materials and techniques. Herein, a post-modified zeolitic imidazolate framework (pm-ZIF) was synthesized by using ZIF-67 as a parent MOF, and zinc(II) meso-tetra (4-carboxyphenyl) porphine (ZnTCPP) as a successive exchange ligand. Due to the preservation of the tetrahedral Co–N4 units from the ZIF precursor and the introduced porphyrin luminophores, this hybrid material pm-ZIF/P(Zn) enables the linear electrochemiluminescence (ECL) signal conversion of the target DNA concentration. An efficient biosensor that can be used to quantitatively detect SARS-CoV-2 was therefore constructed. The linear range of the sensor was 10−12–10−8 M, with a limit of detection (LOD) reaching 158 pM. Compared with the traditional amplification-based methods, the duration time of our method is significantly shortened and the quantitation of the SARS-Cov-2 RdRp gene can be completed within twenty minutes at room temperature.
研究领域:
材料科学
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生物医学工程
·
病毒学
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产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Zhikang Rao,Boyu Guo,Jixiang Zu,Weiqiang Zheng,Ying Xu,Yuting Yang
In clinical diagnosis, the detection of dopamine (DA) is often interfered by epinephrine (EP), a neurotransmitter drug that shares a similar structure with DA, which affects the accurate prediction of DA concentration. In this study, an electrochemiluminescence (ECL) and differential pulse voltammetry (DPV) dual model biosensor combined with machine learning algorithm was proposed for improving the selective detection of DA under EP interference. First, reduced graphene and gold nanoparticles were applied to enhance the sensitivity and stability of the sensor in ECL and DPV measurement. Compared with single model sensing, the dual model sensor could combine the superiority of ECL and DPV while improving the accuracy and reliability of experimental data. Then, nine graphic features related to concentration prediction were extracted from the ECL and DPV response curves, and Pearson’s correlation coefficient (PCC), as well as random forest (RF) algorithm, was adopted for feature importance analysis. To achieve accurate signal decoupling, an intelligent biologically inspired algorithm, particle swarm optimization (PSO) algorithm, was introduced to optimize initial weights and thresholds of the traditional artificial neural network (ANN) for further model training based on multi-feature fusion, which greatly increased the prediction accuracy of DA in the presence of EP. The results demonstrated that DA could be accurately predicted in the concentration range of 9– 200 μM , and the R2 between the true and predicted values of DA concentration reached 0.99. The root-mean-square error (RMSE) and mean absolute error (MAE) of the test dataset were 5.77 and 4.32 μM , respectively. In addition, the PSO-ANN-assisted ECL-DPV dual model detection scheme has been successfully applied for the determination of DA in real samples, such as goat serum and artificial urine.
研究领域:
生物医学工程
·
临床诊断
·
电化学传感
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Jun-Tao Cao,Xiang-Mei Liu,Yi-Zhuo Fu,Shu-Wei Ren,Yan-Ming Liu
A spatial- and potential-resolved ratiometric electrochemiluminescent (ECL) immunosensor was developed for prostate specific antigen detection in a label-free mode. CdS quantum dots (CdS QDs) and luminol-gold nanoparticles (luminol-Au NPs) were the potential-resolved electrochemiluminescence emitters and H2O2 was used as a co-reactant. The cathodic signal from the CdS QD electrode served as the working signal and the anodic signal from luminol-Au NPs as the reference. For prostate specific antigen detection, several concentrations of prostate specific antigen were immobilized on the CdS QDs modified electrode and a fixed concentration of the prostate specific antigen was modified on the luminol-Au NPs modified electrode. The prostate specific antigen levels were quantified by the electrochemiluminescence signal ratio of the cathodic and anodic (ECLcathodic/ECLanodic). Under the optimized conditions, the ratio of the cathodic and anodic signals (ECLcathodic/ECLanodic) had a linear relationship with the logarithm of (C, g mL−1) of prostate specific antigen concentration from 1.0 × 10−11 to 1.0 × 10−7 g mL−1. The detection limit was 5.0 × 10−12 g mL−1. Prostate specific antigen was determined in human serum and the recoveries were from 80.0% to 107.0%. The label-free electrochemiluminescence immunosensor offers simplicity and reliability for human serum with potential for clinical application.
研究领域:
分析化学
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生物传感器
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纳米技术
·
医学诊断
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yuanxun Gong,Shunjin Wen,Pengyuan He,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
s Human chorionic gonadotropin (hCG) is a key clinical marker for early pregnancy and trophoblastic disease. We present a turn-on electrochemiluminescence (ECL) biosensor emphasizing materials safety and aqueous compatibility by integrating a CsPbBr₃@COF-LZU1@Au hybrid with a peptide recognition layer. Confining perovskite nanocrystals inside COF-LZU1 yields a porous, mechanically robust shell that suppresses lead ion release, improves water compatibility/dispersion, and mitigates photochemical and hydrolytic degradation, enabling reliable ECL in PBS. A conformal Au nanodomain rim enhances interfacial charge transport and provides mild, covalent (Au S) anchoring sites for the hCG-binding peptide (PPLRINRHILTR-GGGGC), avoiding bulky antibodies and harsh coupling reagents. Signal regulation is achieved with citrate-stabilized Ag nanoparticles that assemble near the peptide in the intact state to quench ECL; specific hCG binding sterically prevents AgNP tethering, restoring emission. Operating under benign aqueous conditions (no organic solvents during measurement, small reagent volumes, room-temperature assembly), the sensor delivers a wide working range (0.05–5000 mIU/mL), a low detection limit (0.047 mIU/mL), high selectivity over common interferents (FSH, LH, TSH, IgG, BSA), excellent reproducibility (RSD ≈ 2.2 %), and strong intraday/long-term/operational stability. Serum tests give ~96–103 % recoveries with low RSDs, indicating minimal matrix effects. By encapsulating lead-based emitters to reduce toxicity risk, enhancing aqueous dispersibility, and using compact peptide ligands for precise molecular recognition, this modular perovskite@COF/Au/peptide design offers a practical and safer route to high-performance ECL diagnostics and can be readily adapted to additional biomarkers by swapping the peptide sequence.
研究领域:
分析化学
·
生物传感器与生物电子学
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纳米材料
·
临床诊断
·
电分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Qisheng Luo,Chuanhua Zheng,Yongning Huang,Linghuan Liang,Yuanxun Gong,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
Lead-halide perovskites offer bright electrochemiluminescence (ECL) but suffer from aqueous instability and Pb 2+ safety concerns. We construct a CsPbBr 3 @PDA–Au nanointerface that couples polydopamine (PDA) passivation with Au-assisted charge transfer, integrated into a peptide-to-CRISPR/Cas12a amplification scheme for ultrasensitive detection of matrix metalloproteinase-2 (MMP-2). PDA conformally coats CsPbBr 3 , suppressing trap-mediated quenching and mitigating Pb 2+ release, while providing catechol/amine anchors for a tetrahedral DNA scaffold bearing a ferrocene-labeled hairpin. Upon MMP-2 cleavage of a GPLG↓VRG peptide–DNA chimera, the released strand activates Cas12a trans-cleavage, removing the proximal quencher and switching the interface from “off” to “on.” Under unified processing (baseline subtraction and renormalization), the platform achieves a 5.6 aM detection limit with high selectivity over non-target proteases and excellent inter-device reproducibility (triplicate error bars reported). TEM/HRTEM visualize uniform nanocubes; XPS/FTIR evidence PDA catechol/quinone/amine coordination; XRD confirms phase-pure CsPbBr 3 ; and corrected CV/EIS analyses verify accelerated interfacial kinetics after Au decoration. The sensor retains ∼98 % intra-day and ≥91 % 7-day signal, supporting operational robustness. By integrating interfacial stabilization, catalytic electron transfer, and molecular amplification, this modular design advances eco-conscious, perovskite-based ECL diagnostics for low-abundance protease monitoring and broader clinical bioanalysis.
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yanan Li,Peilin Wang,Yixin Nie,Qiang Ma
In this work, a novel sensing interface has been constructed based on the confined gold atoms-MXene nanosheet. In order to improve the sensing performance of MXene, high photoelectric active confined gold atoms-MXene nanosheet were prepared as electrochemiluminescence (ECL) emitter unit firstly. Based on the electronic metal − support interaction, gold single atoms confined on MXene can provide more active sites and efficient electron transfer to generate strong luminescence signal. Furthermore, in order to improve the biocompatibility, an ECL sensing interface with the Au atoms-MXene nanosheet and chitosan was constructed via the layer-by-layer self-assembly (LBL). Therefore, the proposed sensing interface possessed the large specific surface area, good biocompatibility, high electrocatalytic activity and strong ECL signal. As a potential biomarker, the elevated myeloperoxidase (MPO) concentration and activity were associated with many pathological factors to assess the risk of respiratory diseases. To expand the application of this ECL sensing interface in clinical diagnosis, MPO activity was detected in saliva samples. The satisfactory results indicated the confined gold atoms-MXene-based sensing interface possessed great potential in the ECL research and clinical analysis.
研究领域:
生物传感器
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Research
·
2023
·
IF 11
Cheng Ma,Xiaodan Gou,Zejing Xing,Min-Xuan Wang,Wenlei Zhu,Qin Xu,Dechen Jiang,Jun-Jie Zhu
Electrochemiluminescence (ECL) has established itself as an excellent transduction technique in biosensing and light-emitting device, while conventional ECL mechanism depending on spontaneous emission of luminophores lacks reversibility and tunable emission characters, limiting the universality of ECL technique in the fields of fundamental research and clinical applications. Here, we report the first observation of stimulated emission route in ECL and thus establish a reversible tuning ECL microscopy for single-cell imaging. This microscopy uses a focused red-shifted beam to transfer spontaneous ECL into stimulated ECL, which enables selective and reversible tuning of ECL emission from homogeneous solution, single particles, and single cells. After excluding other possible competitive routes, the stimulated ECL emission route is confirmed by a dual-objective system in which the suppressed spontaneous ECL is accompanied by the enhanced stimulated ECL. By incorporating a commercial donut-shaped beam, the sharpness of single-cell matrix adhesion is improved 2 to 3 times compared with the counterpart in confocal ECL mode. The successful establishment of this stimulated emission ECL will greatly advance the development of light-emitting device and super-resolution ECL microscopy.
研究领域:
生物传感
·
发光器件
·
基础研究
·
临床应用
·
超分辨率显微镜
✓ 已验证
DOI
高影响因子
Small
·
2019
·Vol. 15
·期 48
·1901550
·
IF 10.856
Ruina Wang,Haishan Wu,Rui Chen,Yuwu Chi
Carbon nanotubes (CNTs) as well-known nanomaterials are extensively studied and widely applied in various fields. Nitric acid (HNO 3 ) is often used to treat CNTs for purification purposes and preparing oxidized CNTs for various applications. However, too little attention is paid to investigating the effect of HNO 3 treatment on the optical properties of CNTs. In this work, it is observed for the first time that HNO 3 -oxidized multiwalled carbon nanotubes (ox-MWCNTs) have strong electrochemiluminescence (ECL) activity, which enables ox-MWCNTs to become new and good ECL carbon nanomaterials after carbon quantum dots (CQDs) and graphene quantum dots (GQDs). Various characterization technologies, such as transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy, are used to reveal the relationship between ECL activity and surface states. The ECL behaviors of ox-MWCNTs are investigated in detail and a possible ECL mechanism is proposed. Finally, the new ECL nanomaterials of ox-MWCNTs are envisioned to have promising applications in sensitive ECL sensing and in the study of CNT-based catalysts.
研究领域:
材料科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 3
·816-822
·
IF 2.726
Hu Shifan,Wang Yafeng,Su Bin
Electrochemiluminescence(ECL) is a powerful transduction technique used in biosensing and in vitro diagnosis, while the mechanism of ECL generation is complicated and affected by various factors. Herein the effect of ionic strength on ECL generation by the classical tris(2,2′-bipyridyl)ruthenium(II)[Ru(bpy) 3 2+ ]/tri- n -propylamine(TPrA) system was investigated. It is clear that the ECL intensity decreases significantly with the increase of ionic strength, most likely arising from the reduced deprotonation rate of TPrA +· . We further combined microtube electrode(MTE) with ECL microscopy to unravel the evolution of ECL layer with the variation of ionic strength. At a low concentration of Ru(bpy) 3 2+ , the thickness of ECL layer(TEL) nearly kept unchanged with the ionic strength, indicating the surface-confined ECL generation is dominated by the oxidative-reduction route. While at a high concentration of Ru(bpy) 3 2+ , ECL generation is dominated by the catalytic route and TEL increases remarkably with the increase of ionic strength, because of the extended diffusion length of Ru(bpy) 3 3+ at a reduced concentration of TPrA · .
研究领域:
分析化学
·
生物传感
·
电化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Wang,Shiyu He,Mingfu Ye,Yongping Dong
The inherent water insolubility of iridium complexes seriously restricts their application in aqueous electrochemiluminescence (ECL) sensing systems. In order to solve this problem, this study innovatively used water-soluble polymer polyvinyl alcohol to encapsulate the iridium complex ([Ir(dFppy) 2 (d(t-Bu)bpy)](PF 6 )) in a hydrogel, and successfully obtained nanosheets of iridium complex (IrNS) with good dispersion. Systematic studies on the behavior of IrNS ECL in aqueous media showed strong and stable cathodic ECL when persulfate (S 2 O 8 2− ) was used as a co-reactant. Notably, the introduction of rhenium disulfide microspheres (ReS 2 MS) can produce significant cathodic ECL enhancement effects by efficiently catalyzing the conversion of S 2 O 8 2− to generate more SO 4 − . Bisphenol A (BPA) can react with S 2 O 8 2− to reduce the ECL intensity, based on which an ECL sensor was constructed for its detection. Under optimal conditions, ECL intensity varied linearly with BPA concentration, ranging from 1.0 × 10 −10 to 1.0 × 10 −5 mol L −1 with a detection limit of 5.5 × 10 −12 mol L −1 . The fabricated sensors with high sensitivity and selectivity provide a new avenue for the application of water-insoluble iridium complexes in ECL sensing.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）