SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Min Chen,Yang Li,Rui Han,Qiuji Chen,Lingyun Jiang,Xiliang Luo
Herein, an electrochemical immunosensor capable of detecting targets in human serum with ultralow fouling and high sensitivity was successfully constructed through a click reaction. One designed biotinylated peptide was associated with the other peptide functionalized with biotin and azide groups at its two terminals, to form a branched antifouling peptide through the streptavidin-biotin affinity interaction, and it was attached to the electrode to construct an antifouling interface. Then, the exposed azide group on the branched peptide was used to link the antibody modified with 5′-dibenzocyclooctyne (DBCO), and the immunosensor was thus constructed with the aid of the click reaction between the azide group and the DBCO. The developed antifouling immunosensor demonstrated a wide linear response range for carcinoembryonic antigen (CEA) and a low limit of detection of 40 fg mL −1 , which is much superior to the sensing performance of the immunosensor prepared through the normal EDC/NHS method. The immunosensor was capable of assaying CEA in undiluted serum samples with satisfying accuracy when compared with a clinically approved method. This work offered an effective strategy to develop various biosensors through the conjugation of antifouling peptides with different antibodies.
研究领域:
分析化学
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生物化学
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生物传感器
·
电化学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2023
·
IF 6.2
Qianwen Yue,Cai Wang,Ke Zhang,Huiyu Wan,Baoliang Sun,Jingguo Wu,Jingyi Sun,Ying Wang
Thoracolumbar fascia (TLF) plays an important role in the biomechanics of the spine. TLF often causes low back pain (LBP), leading to limb movement disorders and numbness. At present, the clinical diagnosis mainly depends on doctors' clinical experience and magnetic resonance imaging. In order to make the diagnosis more convenient and accurate, we designed a "sandwich" biosensor based on surface-enhanced Raman scattering (SERS) to detect C-reactive protein (CRP) and serum amyloid A (SAA) in fascia. In this study, Ag nanocubes (AgNCs) were used as the substrate, so that the antibodies of CRP and SAA were modified on the substrate surface. Then substrate combined with antigen to form the bottom layer of the sandwich. 4-Mercaptobenzoic acid (4-MBA) and Nairn blue chloride (NBA) were used as signalling molecule probes. Hollow gold nanoparticles (HGNPs) modified with (EDC / NHS) enabled the attachment of antibodies to CRP and SAA to the surface of HGNPs. Eventually, the complex formed by the antigen-antibody reaction reacted with the sandwich substrate after the modification reaction to form the final "sandwich" structure. We successfully applied it to the specific detection of inflammatory markers in fascial tissue of TLF. The results showed that it had high stability and repeatability, which can bring some help for clinical work.
研究领域:
生物传感器
·
医学诊断
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纳米技术
·
炎症标志物
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Xiaomei Dai,Meijuan Yuan,Yuxia Yang,Mengbo Dang,Jian Yang,Junli Shi,Dianwei Liu,Mingjun Li,Hang Yao,Wenyong Fei
The tendon-bone interface heals through scar tissue, while the lack of a natural interface gradient structure and collagen fibre alignment leads to the occurrence of retearing. Therefore, the promotion of tendon healing has become the focus of regenerative medicine. The purpose of this study was to develop a gradient COL1/ hydroxyapatite (HAp) biomaterial loaded with human amniotic mesenchymal stem cells (hAMSCs). The performance of common cross-linking agents, Genipin, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), and dual cross-linked materials were compared to select the best cross-linking mechanism to optimize the biological and mechanical properties of the scaffold. The optimal COL1/HAp-loaded with hAMSCs were implanted into the tendon-bone rotator cuff interfaces in rats and the effect on the tendon-bone healing was assessed by micro-CT, histological analysis, and biomechanical properties. The results showed that Genipin and EDC/NHS dual cross-linked COL1/HAp had good biological activity and mechanical properties and promoted the proliferation and differentiation of hAMSCs. Animal experiments showed that the group using a scaffold loaded with hAMSCs had excellent continuity and orientation of collagen fibers, increased fibrocartilage and bone formation, and significantly higher biomechanical functions than the control group at the interface at 12 weeks post operation. This study demonstrated that dual cross-linked gradient COL1/HAp-loaded hAMSCs could promote interface healing, thereby providing a feasible strategy for tendon-bone interface regeneration.
研究领域:
再生医学
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组织工程
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生物材料
·
干细胞生物学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Liming Ge,Mingjin Zhu,Xinying Li,Yongbin Xu,Xiaonan Ma,Rong Shi,Defu Li,Changdao Mu
It is significant to develop new approaches to incorporate naturally active agents into gelatin-based edible films for effective antioxidant and long-term antibacterial activities. Here the naturally active compound rosmarinic acid (RosA) was firstly conjugated onto gelatin backbone via EDC/NHS chemistry. Then the conjugates were plasticized by glycerol and crosslinked by dialdehyde xanthan gum to fabricate active RosA-gelatin edible films. The results indicate that the RosA-gelatin edible films present good water resistance property, strong mechanical property and excellent ultraviolet light barrier ability. The conjugation of RosA confers gelatin-based edible films with effective antioxidant and antibacterial activities as expected. It is interesting that RosA-gelatin edible films after 3 months of storage still show good antibacterial activity as that of newly prepared ones, demonstrating the long-term antibacterial activity. Overall, the fabricated RosA-gelatin edible films may have promising application in the fields of food and pharmaceutical packaging.
研究领域:
材料科学
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食品科学
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药学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 12
·3159-3170
·
IF 2.137
Kai Huang,Xiao Quan,Xipeng Li,F. Handan Tezel,Baoan Li
Organic fouling has been a major impediment for the widespread application of nanofiltration membranes because it could degrade the performance and shorten the service life of such membranes. In this study, the NH 2 -functionalized silica nanoparticles (NH 2 –SiO 2 NPs) were grafted on the surface of commercial NF90 membrane to improve the hydrophilicity and antifouling property with the N -(3-dimethylaminopropyl)- N -ethylcarbodiimide hydrochloride coupled with the N -hydroxysuccinimide (EDC/NHS) to activate carboxyl groups on the membrane surface. The surface roughness (Ra) of this modified membrane decreased from value of 47.4 nm of the pristine membrane to 43 nm. The water contact angle of the modified membrane decreased from 48.4° to 15°, indicating the high hydrophilicity of the surface. Moreover, the EDC/NHS membrane showed good stability when it was exposed to the ruinous physical stress (2-min sonication). The optimized grafting conditions, including the SiO 2 concentration (0.5 wt%), the activation time of EDC/NHS (20 min), and the reaction time of NH 2 –SiO 2 NPs with membrane surface (12 h), were obtained by a series of experiments. Under the antifouling test of 36 hours with bovine serum albumin (BSA) and hexadecyltrimethylammonium bromide (CTAB) as foulants, the EDC/NHS membrane lost 42.1% and 49.6% of its initial flux, respectively, which is lower than that of the pristine membrane (52.2% and 68%, respectively). After cleaning with deionized water for 2 hours, the EDC/NHS membrane also showed significant improvement of flux recovery ratio (92% and 87%, respectively) than did the pristine membrane (70.3% and 60%, respectively).
研究领域:
环境工程
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膜科学
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yi Zhang,Yuanxiang Huang,Zhenfeng Yue,Huajun Fan,Shaojing Wu
A novel, efficient and specific aptamer-functionalized sorbent was successfully synthesized and applied in the analysis of ultra-trace aflatoxin M1 (AFM1) and its analogues in milk. By using a gold nanoparticle-based colorimetric analysis tool, we could determine that the AFM1-aptamer (M1-aptamer) recognized and bound AFM1 to generate an aptamer-target complex. After analysis and verification, the M1-aptamer on the surface of the carboxyl microspheres were completely modified via EDC/NHs amide formation. The effects of extraction pH, extraction buffer, concentration of Mg 2+ and elution solvents were investigated by single-factor experiments to determine the most suitable analysis conditions. Using these conditions, the extraction performance of the M1-aptamer-functionalized sorbent could be studied and an analytical method for AFM1 and its analogues in milk could be established. The results of adsorption equilibrium as well as adsorption selectivity experiments indicated that the M1-aptamer offered a specific adsorption site to selectively adsorb aflatoxins exhibiting a dihydrofuran oxaphthalene scaffold. The adsorption capacity of the aptamer-functionalized sorbent was 233.1 μg/g. A studied method for the determination AFM1 in milk with low matrix effect demonstrated a good linear correlation in the range of 0 to 2.0 μg/L (R 2 = 0.9982) and high detection sensitivity (detection limits of 7 ng/kg in milk and 21 ng/kg in milk power). Furthermore, excellent spiked recovery and good repeatability were obtained. The method delivered specific, accurate and reproducible results for enriching and detecting ultra-trace AFM1 and its analogues in milk.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2016
·Vol. 56
·期 12
·1432-1436
·
IF 1.719
Shiping Zhan,Nan Liu,Weijing Wang,Shuhua Chen,Jingchang Wang
To improve the properties of chitosan (CS) and poly(L-lactic acid) (PLLA) and obtain fully biodegradable materials, CS- g -PLLA copolymers were prepared using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) as a coupling agent. The copolymers were characterized by Fourier transform infrared analysis (FTIR), 1 H nuclear magnetic resonance ( 1 H NMR), elemental analysis, differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results obtained by FTIR and 1 H NMR showed that CS and PLLA were grafted successfully via an amide bond. DSC and XRD results showed that the thermal stability of CS had been significantly improved by grafting PLLA to the molecular chains of CS and the crystallinity of the CS- g -PLLA copolymers decreased significantly. Elemental analysis showed that the achieved the maximum degree of substitution of PLLA was 60.88%, while the concentration of CS was 2 mg/mL, the PLLA molecular weight was 10,000, and the EDC/NHS ratio was 2:1. Images from SEM demonstrated that the copolymers had a spherical shape and smooth surface. Moreover, the products were well dispersed without any aggregation. POLYM. ENG. SCI., 56:1432–1436, 2016. © 2016 Society of Plastics Engineers
研究领域:
高分子科学
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材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 4
·758-766
·
IF 5.047
Dong Han,Baolin Zhang,Chuangang Chong,Cuiping Rong,Jie Tan,Rusen Yang
Effective attachment of magnetic nanoparticles to neuronal membranes has far-reaching significance in activating ion channels and treating neurodegenerative diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) synthesized by the polyol pyrolysis method have the advantages of rich surface functional groups, excellent magnetic properties, controllable particle size and water dispersibility. We propose that perfusion of biotin into the targeted brain area should be initially performed because it tends to be adsorbed by cell membranes, followed by injection of streptavidin (SA)-modified SPIONs into the same area of the brain. By means of the strong binding force between SA and biotin, the SPIONs may subsequently adhere to the cell surfaces in the brain area. In this work, fluorescein isothiocyanate-streptavidin (FITC-SA) was modified on the surface of polyethylene imine (PEI)-SPIONs by the EDC–NHS method and stereotaxically injected into the biotin-supplemented substantia nigra of mice. The combination of fluorescence detection with transmission electron microscopy (TEM) confirmed that FITC-SA/PEI-SPIONs adhered to neuronal membranes in the substantia nigra of mice 24 h after injection. The results show that our strategy can promote the attachment of SPIONs to neuronal membranes.
研究领域:
神经科学
·
纳米技术
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2019
·Vol. 7
·期 47
·27156-27163
·
IF 10.733
Weifeng Zhang,Ruixiang Qu,Xiangyu Li,Yanan Liu,Yen Wei,Lin Feng
In the oil/water emulsion treatment field, the separation of dispersed tiny oil droplets and the removal of surfactants are both significant in order to obtain purified water. Superwetting materials have proved to be an ideal solution for stabilized emulsion separation. However, most of the previous studies only focus on the separation of oil from water while neglecting the removal of surfactants. With the purpose of realizing in situ emulsion separation and surfactant removal, a dual functional Janus membrane is designed inspired by mussel-inspired chemistry and an EDC/NHS coupling reaction. After modification, abundant amino groups and carboxyl groups are decorated on two sides of the microfiltration membrane substrate, respectively (denoted as the –NH2 side and –COOH side). Combining special wettability with electrostatic interaction, the as-prepared membrane is able to separate stabilized oil-in-water emulsions and remove anionic/cationic surfactants simultaneously. The –NH2 side is used for the treatment of anionic emulsions with the surfactant sodium dodecyl sulfate (SDS), while the –COOH side is used for the treatment of cationic emulsions with the surfactant hexadecyl trimethyl ammonium bromide (CTAB). More importantly, the separation efficiencies are all above 99.5% and the surfactant removal efficiencies are up to 95.7%. To our knowledge, this is the first time to treat both dispersed oil droplets and surfactants using superwetting materials. The excellent recyclability and stability of the Janus material make it a potential candidate for application in the industrial field.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Leilei Sun,Bafang Li,Dandan Jiang,Hu Hou
Nile tilapia skin collagen sponges were fabricated by freeze-drying technology and modified with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide (EDC/NHS), genipin + PBS, genipin + ethanol, tea polyphenol (TP), nordihydroguaiaretic acid (NDGA) and diphenyl phosphoryl azide (DPPA). Physicochemical and biological properties, micromorphology and compatibility before and after modification were investigated to evaluate collagen sponge as a hemostatic biomedical material. The mechanical property of collagen sponges strengthened after cross-linking. The elongation at break of cross-linked collagen sponges decreased except for EDC/NHS, which was close to that of non-crosslinked. The collagen sponge cross-linked with EDC/NHS exhibited the highest hygroscopicity in comparison with other cross-linkers. The resistance to collagenase biodegradation of collagen sponges after cross-linking strengthened significantly except for NDGA. Collagen sponges cross-linked with EDC/NHS, TP and NDGA maintained high porosity (97–98%), similar to non-crosslinked (98.42%). Collagen sponges could shorten the blood coagulation time. From the variations of the FTIR spectrum pattern and SEM, DPPA could change the secondary structure of collagen and destroy the spongy structure of collagen sponge, which was not suitable for the cross-linking of collagen sponge. Whereas, EDC/NHS was recognized as a perfect cross-linker owing to its excellent properties and porous microstructure. All fabricated collagen sponges were recognized to be biocompatible by the hemolysis assay in vitro . Therefore, collagen sponge modified with EDC/NHS could be used as a perfect biomedical hemostatic material.
研究领域:
生物医学工程
·
材料科学
·
生物材料
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2019
·Vol. 7
·期 6
·2383-2393
·
IF 5.251
Wanyun Song,Xinyi Li,Yuquan Zhao,Chaoying Liu,Junying Xu,Huiyu Wang,Tao Zhang
The capture of circulating tumor cells (CTCs) plays a crucial role in the early diagnosis, personalized treatment and postoperative evaluation of malignant tumors. In this study, UV-curable coating technology was combined with antibody immobilization to enable CTC captures on poly(methyl methacrylate) (PMMA) substrates. Controlled amounts of carboxyl groups and polyethylene glycol (PEG) segments were introduced into the coating formulation to facilitate immobilization of antibodies and block non-specific protein adsorption, respectively. Then, anti-EpCAM antibodies were immobilized on functionalized, coated PMMA substrates by EDC/NHS chemistry. Multiple physical, chemical and biological properties were investigated, including hydrophilicity, protein adsorption, platelet adhesion and anticoagulant properties. Thereafter, optimized coatings were applied on the inner wall of PMMA tubes, followed by immobilization of anti-EpCAM antibodies. After perfusion of the tubes with whole blood, enriched with SGC7901 gastric cancer cells that overexpress EpCAM antigens, rapid and efficient capture of the tumor cells was observed. These results provide a basis for further development of devices for the selective capture and enrichment of CTCs, using small blood volumes.
研究领域:
生物医学工程
·
癌症研究
·
材料科学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 7
·期 1
·336-346
·
IF 5.831
Dong Chen,Huangyong Jiang,Dongbo Guo,Wumaier Yasen,Junping Ao,Yue Su,Dongsheng Pan,Xin Jin,Xinyuan Zhu
Cationic gelatin nanoparticles ((+)nGNPs) were prepared by in situ polymerization upon the surfaces of monodispersed gelatin nanoparticles (GNPs) using N-(3-Aminopropyl)methacrylamide (APm) as monomer, which were then decorated with doxorubicin terminated poly(2-methylacryloyloxyethyl phosphorylcholine) (DOX-pMPC) via EDC/NHS conjugation to obtain core–shell nanoparticles ((+)nGNPs@DOX-pMPC) for cancer therapy. The non-fouling pMPC shell could effectively shield the positively charged surface of inner nanoparticle and prevent non-specific protein adsorption, thus endowing the materials with potential for long-acting cancer treatment. Furthermore, the acyl hydrazone bond connecting DOX and pMPC chain could be easily hydrolyzed in the weakly acidic tumor microenvironment. After decladding of the pMPC shell, electropositive (+)nGNPs carrying the drugs can be effectively internalized by cancer cells to induce apoptosis, avoiding undesirable hindrance caused by the superhydrophilic outer layer. On combining the above properties, this drug delivery system can be a promising candidate for long-acting, low-toxicity and high-efficiency cancer therapy.
研究领域:
纳米技术
·
药学
·
癌症治疗
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）