JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2025
·Vol. 113
·期 9
·e35646
·
IF 3.4
Huiwen Wang,Yuting Wu,Mingrui Dai,Tingting Zhu,Daming Wu,Diya Leng
To investigate the antibacterial effect, mechanism, and cytotoxicity of Prussian blue/Cerium dioxide (PB/CeO 2 ) nanoparticles against Enterococcus faecalis (E. faecalis) and biofilm. PB/CeO 2 nanoparticles were synthesized and characterized. The antibacterial mechanism of nanoparticles was explored through peroxidase (POD) activity assay, hydroxyl radicals (·OH) detection, and measurement of bacterial reactive oxygen species (ROS) and glutathione (GSH)/glutathione disulfide (GSSG) levels. The biocompatibility of PB/CeO 2 was evaluated by Cell Counting Kit-8 (CCK-8) assay and histological examination of the major visceral organs of rats. The antibacterial effect of PB/CeO 2 was assessed using the colony-forming unit (CFU) method. The impact of PB/CeO 2 on E. faecalis biofilm on dentin slices was further observed with CLSM and SEM. ANOVA and t -test were applied for statistical analysis ( p < 0.05). PB/CeO 2 demonstrated significant antibacterial activity against E. faecalis , mainly when used with H 2 O 2 , significantly enhancing its antibacterial effect and effectively disrupting E. faecalis biofilms on dentin slices. PB/CeO 2 nanoparticles catalyzed ROS production, disrupting the antioxidant defense system of E. faecalis cells, damaging bacterial cell membranes, and ultimately causing bacterial death. PB/CeO 2 nanoparticles exhibit good biocompatibility at appropriate concentrations in vivo and in vitro. The novel multifunctional nanocomposite shows great antibacterial effects against E. faecalis and its biofilm, with low cytotoxicity and good biocompatibility, offering a novel disinfection strategy for root canal treatment.
研究领域:
纳米技术
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微生物学
·
牙科
·
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2018
·Vol. 16
·期 1
·1-16
·
IF 5.294
Fan Wei,Sun Qing,Li Yanyun,Tay Franklin R.,Fan Bing
Background Ag + and Zn 2+ have already been used in combinations to obtain both enhanced antibacterial effect and low cytotoxicity. Despite this, it is still unclear how the Zn 2+ co-works with Ag + in the synergistic antibacterial activity. The main purposes of this study were to investigate the co-work pattern and optimum ratio between Ag + and Zn 2+ in their synergistic antibacterial activity against E. faecalis , the possible mechanisms behind this synergy and the primary application of optimum Ag + –Zn 2+ co-work pattern against the E. faecalis biofilm on dentin. A serial of Ag + –Zn 2+ atomic combination ratios were tested on both planktonic and biofilm-resident E. faecalis on dentin, their antibacterial efficiency was calculated and optimum ratio determined. And the cytotoxicity of various Ag + –Zn 2+ atomic ratios was tested on MC3T3-E1 Cells. The role of Zn 2+ in Ag + –Zn 2+ co-work was evaluated using a Zn 2+ pretreatment study and membrane potential—permeability measurement. Results The results showed that the synergistically promoted antibacterial effect of Ag + –Zn 2+ combinations was Zn 2+ amount-dependent with the 1:9 and 1:12 Ag + –Zn 2+ atomic ratios showing the most powerful ability against both planktonic and biofilm-resident E. faecalis. This co-work could likely be attributed to the depolarization of E. faecalis cell membrane by the addition of Zn 2+ . The cytotoxicity of the Ag + –Zn 2+ atomic ratios of 1:9 and 1:12 was much lower than 2% chlorhexidine. Conclusions The Ag + –Zn 2+ atomic ratios of 1:9 and 1:12 demonstrated similar strong ability against E. faecalis biofilm on dentin but much lower cytotoxicity than 2% chlorhexidine. New medications containing optimum Ag + –Zn 2+ atomic ratios higher than 1:6, such as 1:9 or 1:12, could be developed against E. faecalis infection in root canals of teeth or any other parts of human body.
研究领域:
微生物学
·
口腔医学
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生物材料学
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DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Ziqi Wang,Yingyue Huang,Miao Yu,Wei Zhuang,Minghao Sui
Antimicrobial resistance (AMR) poses a global health threat to aquatic environments and its propagation is a hot topic. Therefore, deactivating antibiotic-resistant bacteria (ARB) and removing antibiotic resistance genes (ARGs) from water is crucial for controlling AMR transmission. Peracetic acid (PAA), which is known for its potent oxidizing properties and limited by-product formation, is emerging as a favorable disinfectant for water treatment. In this study, we aimed to assess the efficacy of pre-exposure to PAA followed by UV treatment (PAA-UV/PAA) compared with the simultaneous application of UV and PAA (UV/PAA). The focus was on deactivating vancomycin-resistant Enterococcus faecalis (VRE fs ), a typical ARB in water. Pre-exposure to PAA significantly enhanced the efficacy of subsequent UV/PAA treatment. At a UV fluence of 7.2 mJ cm −2 , the PAA-UV/PAA method achieved a 6.21 log reduction in VRE fs , surpassing the 1.29 log reduction observed with UV/PAA. Moreover, compared to UV/PAA, PAA-UV/PAA showed increased efficacy with longer pre-exposure times and higher PAA concentrations, maintaining superior performance across a broad pH range and in the presence of humic acid. Flow cytometry analysis indicated minimal cellular membrane damage using both methods. However, the assessments of superoxide dismutase (SOD) activity and adenosine triphosphate content revealed that PAA-UV/PAA induced greater oxidative stress under similar UV irradiation conditions, leading to slower bacterial regrowth. Specifically, SOD activity in PAA-UV/PAA surged to 3.06 times its baseline, exceeding the 1.73-fold increase under UV/PAA conditions. Additionally, pre-exposure to PAA amplified ARGs degradation and reduced resistance gene leakage, effectively mitigating the spread of AMR. Pre-exposure to 200 μM PAA for 10 and 20 min enhanced vanB gene removal efficiency by 0.14 log and 1.29 log, respectively. Our study provides a feasible approach for optimizing UV/PAA disinfection for efficient removal of ARB and ARGs.
研究领域:
环境科学
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微生物学
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水处理
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抗菌素耐药性
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产品关联
DOI
PubMed
高影响因子
MICROBIAL PATHOGENESIS
·
2025
·
IF 3.5
Tianmeng Li,Hong Shen,Peng Wang,Yu Wang,Jun Liu,Meibo He,Li Gu,Shuai Wang,Ming Wei
Enterococcus faecalis , a prevalent opportunistic pathogen, readily forms biofilms on surfaces, contributing to its virulence and antibiotic resistance. Previous studies have highlighted arachidonic acid (AA) as a promising antibiofilm agent. This investigation aimed to elucidate the antibiofilm activity of AA against linezolid-resistant E. faecalis and explore its potential molecular mechanisms. A total of 21 E. faecalis strains were included in this study: the standard strain ATCC 29212 and 20 linezolid-resistant clinical isolates. To determine the minimum inhibitory concentrations (MICs) of antibiotics and AA, the microbroth dilution method was performed. Checkerboard microdilution assays were also performed to assess the interaction between AA and linezolid. The crystal violet method was performed to evaluate the biofilm formation abilities of E. faecalis and the biofilm inhibition activities of different concentrations of AA (0.25 mM, 0.5 mM, and 1 mM). In addition, the expression levels of biofilm-related genes ( ebpA, ebpB, ebpC, gelE, ace, atlA, and esp ) were measured employing quantitative reverse transcription PCR (qRT-PCR). All clinical strains exhibited resistance to linezolid and tetracycline while remaining susceptible to other antibiotics, including penicillin, ampicillin, vancomycin, teicoplanin, and tigecycline. The MICs of AA against all 21 E. faecalis isolates were >1 mM. Synergy was observed in 75 % of strains (15/20), while additivity was observed in 25 % strains (5/20). All isolates displayed strong biofilm-forming abilities. AA significantly reduced biofilm formation with average inhibition rates of 68.16 %, 69.64 %, and 72.01 % at concentrations of 0.25 mM, 0.5 mM, and 1 mM, respectively ( P < 0.001). AA treatment resulted in decreased expression of ebpB, ebpC, ace, and atlA ( P < 0.001), while simultaneously increasing gelE expression ( P < 0.05). In conclusion, AA exhibited potent inhibitory effects on E. faecalis biofilm formation probably by influencing bacterial adhesion. The addition of AA can restore the susceptibility of linezolid against E. faecalis . These findings suggest that AA may serve as a potential therapeutic agent for preventing and treating E. faecalis infections, particularly those associated with biofilm formation.
研究领域:
微生物学
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抗菌研究
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分子生物学
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传染病
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药理学
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产品关联
DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF MICROBIOLOGY
·
2021
·Vol. 53
·期 1
·161-169
·
IF 2.476
Duan Mengting,Sun Qing,Fan Wei,Fan Bing
Enterococcus faecalis ( E. faecalis ) is commonly considered to be one of chief culprits of secondary and persistent root canal infections. As antibiotic resistance has become a global issue, in order to reduce the use of antibiotics, metal ions have recently been widely used as an alternative. Silver ions (Ag + ) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Triton X-100 (TX-100) and Ag + were co-used for the first time as a clinical intracanal medication to obtain both enhanced antibacterial effect and low cytotoxicity. The synergistic antibacterial effect of TX-100 + Ag + was tested on both planktonic and biofilm-resident E. faecalis on dentine. And the cytotoxicity was tested on MC3T3-E1 cells. Results confirmed the antibacterial activity against both planktonic and biofilm-resident E. faecalis was dramatically improved after TX-100 incorporation. TX-100 and Ag + mixture demonstrated a similar inhibitory effect as the 2% chlorhexidine (CHX), while the cytotoxicity was much lower than 2% CHX ( p < 0.05). In conclusion, TX-100 + Ag + mixture might be developed into a new effective intracanal medication as the 2% CHX.
研究领域:
口腔医学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2019
·Vol. 30
·期 2
·1-11
·
IF 2.467
Fan Wei,Li Yanyun,Liu Danfeng,Sun Qing,Duan Mengting,Fan Bing
Enterococcus faecalis ( E. faecalis ), a Gram-positive facultative anaerobe, is reported to take responsibility for a large portion of refractory root canal infections and root canal re-infections of human teeth. Chlorhexidine is a strong bactericide against E. faecalis but cannot infiltrate into dentinal tubules. On the other hand, a common negative effect of root canal medicaments is the decrease of dentin microhardness. In this study, poly(D,L-lactic- co -glycolide) (PLGA) submicron particles were applied as delivery carriers to load and release the chlorhexidine as well as calcium and phosphorus. The release profiles, antibacterial ability against E. faecalis , infiltration ability into dentinal tubules, biocompatibility and effects on dentin microhardness of these particles were investigated. Results revealed that encapsulated chemicals could be released in a sustained manner from the particles. The particles also exhibited excellent biocompatibility on MC3T3-E1 cells and significant antimicrobial property against E. faecalis . On dentin slices, the particles could be driven into dentinal tubules by ultrasonic activiation and inhibit E. faecalis colonization. Besides, dentin slices medicated with the particles displayed an increase in microhardness. In conclusion, PLGA submicron particles carrying chlorhexidine, calcium and phosphorus could be developed into a new intra-canal disinfectant for dental treatments.
研究领域:
牙科学
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药学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Oral Health
·
2023
·Vol. 23
·期 1
·1-10
·
IF 2.9
Lv Silei,Fan Wei,Fan Bing
Background Enterococcus faecalis (E. faecalis) is frequently isolated from root canals with failed root canal treatments. Due to the strong ability of E. faecalis to resist many often-used antimicrobials, coping with E. faecalis infections remains a challenge. The aim of this study was to investigate the synergistic antibacterial effect of low-dose cetylpyridinium chloride (CPC) and silver ions (Ag+) against E. faecalis in vitro.Methods The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the fractional inhibitory concentration index (FICI) were used to confirm the existence of the synergic antibacterial activity between low-dose CPC and Ag+. Colony-forming unit (CFU) counting, time-killing curve and dynamic growth curve were used to evaluate the antimicrobial effects of CPC and Ag+ combinations against planktonic E. faecalis. Four weeks biofilms were treated with drug-contained gels to determine the antimicrobial effect on biofilm-resident E.faecalis, and the integrity of E.faecalis and its biofilms were observed by FE-SEM. CCK-8 assays was used to test the cytotoxicity of CPC and Ag+ combinations on MC3T3-E1 cells.Results The results confirmed the synergistic antibacterial effect of low-dose CPC and Ag+ against both planktonic and 4-week biofilm E. faecalis. After the addition of CPC, the sensitivity of both planktonic and biofilm-resident E. faecalis to Ag+ improved, and the combination showed good biocompatibility on MC3T3-E1 cells.Conclusion sLow-dose CPC enhanced the antibacterial ability of Ag+ against both planktonic and biofilm E.faecalis with good biocompatibility. It may be developed into a novel and potent antibacterial agent against E.faecalis, with low toxicity for root canal disinfection or other related medical applications.
研究领域:
口腔医学
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微生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Bin Zong,Xue Li,Quanchen Xu,Danyang Wang,Pengyu Gao,Qihui Zhou
Due to the persistent presence of Enterococcus faecalis biofilms in apical root canals, persistent endodontic infections (PEIs) have always been an intractable disease to solve. The conventional root canal disinfectants (e.g., calcium hydroxide, chlorhexidine) are arduous to scavenge the stubborn infection. With the progress of nanomedicine in the biomedical field, antimicrobial photodynamic therapy (aPDT) is emerging as a prospective anti-infective therapy for PEIs. Herein, quaternized chitosan (QCh) modified upconversion nanoparticles (UCNP)@SiO 2 /methylene blue (MB) are developed with enhanced antibacterial/biofilm performance for aPDT in PEIs. QCh is coated on the UCNP@SiO 2 /MB by testing the changes in diameter, chemical functional group, and charge. Interestingly, QCh also increases the conversion efficiency of UCNP to generate more reactive oxygen species (ROS). Furthermore, the prepared UCNP@SiO 2 /MB@QCh exhibits highly effective antibacterial activity against free E. faecalis and related biofilm in vitro and extracted teeth. Importantly, the additional QCh with positive charges enhance UCNP@SiO 2 /MB@QCh contact with E. faecalis (negative charges) through electrostatic interaction. Then, UCNP@SiO 2 /MB@QCh could stick close to the E. faecalis and generate ROS under the irradiation by a 980 nm laser. The in vitro cellular test shows that UCNP@SiO 2 /MB@QCh has acceptable cytocompatibility. Thus, UCNP@SiO 2 /MB@QCh could offer a novel strategy for the potential aPDT clinical applications in the treatment of PEIs.
研究领域:
纳米医学
·
牙科
·
生物医学工程
·
抗菌治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-16
·
IF 9.429
Tang Shang,Zhang Hui,Mei Li,Dou Keke,Jiang Yuying,Sun Zhanyi,Wang Shuai,Hasanin Mohamed Sayed,Deng Jing,Zhou Qihui
Enterococcus faecalis ( E. faecalis ) biofilm-associated persistent endodontic infections (PEIs) are one of the most common tooth lesions, causing chronic periapical periodontitis, root resorption, and even tooth loss. Clinical root canal disinfectants have the risk of damaging soft tissues (e.g., mucosa and tongue) and teeth in the oral cavity, unsatisfactory to the therapy of PEIs. Nanomaterials with remarkable antibacterial properties and good biocompatibility have been developed as a promising strategy for removing pathogenic bacteria and related biofilm. Herein, carbon dots (CDs) derived from fucoidan (FD) are prepared through a one-pot hydrothermal method for the treatment of PEIs. The prepared FDCDs (7.15 nm) with sulfate groups and fluorescence property are well dispersed and stable in water. Further, it is found that in vitro FDCDs display excellent inhibiting effects on E. faecalis and its biofilm by inducing the formation of intracellular and extracellular reactive oxygen species and altering bacterial permeability. Importantly, the FDCDs penetrated the root canals and dentinal tubules, removing located E. faecalis biofilm. Moreover, the cellular assays show that the developed FDCDs have satisfactory cytocompatibility and promote macrophage recruitment. Thus, the developed FDCDs hold great potential for the management of PEIs. Graphical Abstract
研究领域:
纳米技术
·
牙科
·
生物医学工程
·
微生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 1
·124
·
IF 4.926
Xinling He,Siqi Jin,Wei Fan,Bing Fan
The prevention and treatment of oral diseases is more difficult in diabetic patients with poorly controlled blood glucose levels. This study aims to explore an effective, low-cytotoxicity medication for root canal treatment in diabetic patients. The antibacterial effect of the combination of Triton X-100 (TX-100) and metformin (Met) onEnterococcus faecalis(E. faecalis) was evaluated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration required to kill 99% bacteria (MBC99) and by conducting dynamic time-killing assays. While the antibiofilm activity was measured by crystal violet (CV) assay, field emission scanning electron microscope (FE-SEM), confocal laser scanning microscope (CLSM) and colony-forming unit (CFU) counting assays. The expression of relative genes was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR), and the cytotoxicity of the new combination on MC3T3-E1 cell was also tested. Results showed that the antibacterial and antibiofilm activities of Met could be significantly enhanced by very low concentrations of TX-100 in both normal and high-glucose conditions, with a much lower cytotoxicity than 2% chlorhexidine (CHX). Thus, the TX-100 + Met combination may be developed as a promising and effective root canal disinfectant for patients with diabetes.Keywords:antimicrobial activity;dentinal tubules;Enterococcus faecalis;high-glucose condition;metformin;Triton X-100
研究领域:
牙科学
·
微生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Clinical Oral Investigations
·
2024
·Vol. 28
·期 10
·1-14
·
IF 3.1
Liu Runze,Liu Pei,Luo Yi,Fan Wei,Fan Bing
Objectives The mannose phosphotransferase system (Man-PTS) plays crucial roles in the adaptive metabolic activity of Enterococcus faecalis ( E. faecalis ) in adverse environments. The aim of this study was to evaluate the role of Man-PTS in the alkaline resistance of E. faecalis against calcium hydroxide (CH) and the effect of metformin (Met) on the alkaline resistance of E. faecalis to CH. Materials and methods The regulatory role of Man-PTS EII in the alkaline resistance of E. faecalis was firstly investigated using a wild-type highly alkaline-resistant E. faecalis XS 003, standard ATCC 29212 and Man-PTS EIID gene deficient (△ mptD ) and overexpressing (+ mptD ) strains of E. faecalis . RNA sequencing of Met-treated E. faecalis was performed to further validate the effect of Met on Man-PTS. The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of Man-PTS EII and membrane transporter-related genes of E. faecalis . The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested. The in vivo therapeutic effect of Met plus CH (CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis XS 003. Results Man-PTS EII significantly promoted the survival ability of E. faecalis in CH and enhanced its resistance to CH. The inhibition of Man-PTS EII by Met resulted in reduced alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. Additionally, Met plus CH showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusions Met could significantly reduce the alkaline resistance of E. faecalis against CH through the modulation of Man-PTS EII, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo. Clinical relevance Met could significantly enhance the ability of CH to control E. faecalis infection through reducing the alkaline resistance of E. faecalis .
研究领域:
微生物学
·
药理学
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牙科学
·
传染病学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2022
·
IF 7.033
Xiao Zhao,Ying Wang,Tingting Zhu,Huili Wu,Diya Leng,Zhiguo Qin,Yan Li,Daming Wu
Background Mesoporous calcium-silicate nanoparticles (MCSNs) are advanced biomaterials that have been used to control drug delivery for many years. Ultrasmall Prussian blue nanoparticles (UPBNPs) showed high peroxidase and catalase-like activities. This study evaluated the antibacterial and antibiofilm properties, mechanism and cytotoxicity of UPBNPs-MCSNs composites synthesized by both as precursors. Methods UPBNPs-MCSNs were prepared and characterized. The antibacterial effect of UPBNPs-MCSNs was evaluated by the MTT assay and CFU counting method, and their biosafety was tested by CCK8. Then explore the antibacterial mechanism, including TEM observation of bacterial morphology, and detection of bacterial ROS, LPO and GSH levels. The antibiofilm activity of UPBNPs-MCSNs was tested by E. faecalis biofilm model in human roots. The roots were pretreated with materials and cultured with E. faecalis , and the survival of E. faecalis on the root canal wall was observed by SEM and CLSM. Results The results showed that UPBNPs-MCSNs had potent antibacterial and antibiofilm activities. They can aggregate on the dentin surface and significantly inhibit E. faecalis adhesion and colonization. Their antibacterial activity is as effective as NaClO and calcium hydroxide (CH), can significantly prolong the time of bacterial colonization than CH, but have lower cytotoxicity to normal cells. We found that UPBNPs-MCSNs trigger a like classic ferroptosis pathway in bacteria. UPBNPs-MCSNs can induce bacteria to produce ROS and LPO, and reduce GSH level. Moreover, we observed that the metal ions chelator and the antioxidant could block their antibacterial activity. Conclusion These results reveal that UPBNPS-MCSNs have high antibacterial and antibiofilm, and can mediate the bacterial redox pathway ROS/GSH like the classical pathway of ferroptosis, providing a theoretical basis for them to develop into a safe and effective novel root canal disinfectant.
研究领域:
医学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）