SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Zhitao Chen,Yue Lin,Xiaoming Ma,Longhua Guo,Bin Qiu,Guonan Chen,Zhenyu Lin
Fish product is one of the main seafood with the disadvantage of easy to perish. Various indexes and diverse methods have been developed to evaluate the fish freshness, but most of them are complex and cannot be applied for on-field detection. In this study, we reported a simple, visual and economical multicolor biosensor based on the etching of gold nanorods (GNRs) to evaluate the fish freshness with the naked eye. Hypoxanthine (Hx) is chosen as the index of fish freshness and it react with the dissolved oxygen to produce H 2 O 2 at the presence of xanthine oxidase (XOD). Then GNRs are etched quickly by the H 2 O 2 in the presence of Fe 2+ . Correspondingly, the GNRs surface plasmon resonance (SPR) is regulated and results in a distinctly color change of the system, which can be easily distinguished with the naked eye. Therefore, the concentration of Hx in the fish samples can be semi-quantitatively analyzed with the naked eye. The proposed multicolor Hx sensor has been successfully applied to the detection of Hx in fish samples.
研究领域:
食品科学
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生物传感技术
·
分析化学
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DOI
高影响因子
npj Science of Food
·
2023
·Vol. 7
·期 1
·1-13
·
IF 6.4
Xu Enbo,Niu Ruihao,Lao Jihui,Zhang Shengliang,Li Jie,Zhu Yiyuan,Shi Huimin,Zhu Qingqing,Chen Yijian,Jiang Yuyan,Wang Wenjun,Yin Jun,Chen Qihe,Huang Xiao,Chen Jun,Liu Donghong
Tissue-like cultured meats of some livestock have successfully been established by different approaches. However, the production of a structure similar to fish fillets is still challenging. Here, we develop tissue-like cultured fish fillets by assembly of large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibition of Tgf-β and Notch signals significantly promoted myogenic differentiation of piscine satellite cells (PSCs). The mixture of fish gelatin and sodium alginate combined with a p53 inhibitor and a Yap activator supported PSC viability and proliferation. Based on the texture of fish muscle tissue, a 3D scaffold was constructed by gelatin-based gel mixed with PSCs. After proliferation and differentiation, the muscle scaffold was filled with cultured piscine adipocytes. Finally, tissue-like fish fillets with 20 × 12 × 4 mm were formed, consisting of 5.67 × 10 7 muscles and 4.02 × 10 7 adipocytes. The biomanufacture of tissue-like cultured fish fillet here could be a promising technology to customize meat production with high fidelity.
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PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Yu Xiang-Fang,Gurung Chetali,Yu Zhongjia,Xiao Wei,Kerem Goher,Teng Bin,Zhang Jian V.,Su Zhe,Zhou Yongjin,Ren Pei-Gen
Awareness of the intestinal microflora involved in insulin resistance and type 2 diabetes (T2DM) has now become more evident. However, direct mechanical insight is required to illustrate the contribution of intestinal microflora in the disease progression. In this study, we aimed to precisely assess the changes in intestinal bacteria translocation (IBT) from gut to the pancreas using combinations of FISH, 16S rRNA amplicon sequencing, and deep learning-assisted methods to track IBT in diet-induced obese (DIO) and antibiotic-induced microbiota disruption (AIMD)-DIO mouse models. Our analysis showed deep learning-assisted quantification enhanced the accuracy and objectivity of bacterial tracking. The DIO mice exhibited increased IBT, likely due to excessive intestinal lipid accumulation and compromised intestinal barrier integrity. Elevated bacterial loads in the pancreas were associated with worsened pancreatic function, indicated by higher fasting blood glucose, impaired glucose tolerance, and dysfunctional insulin secretion. In contrast, the AIMD-DIO group lowered the IBT, maintained the islet structure and improved glucose homeostasis. Comparative study between DIO and AIMD-DIO models revealed a strong correlation between number of translocated bacteria and T2DM severity. These findings provide objective evidence of bacterial migration from the intestine to the pancreas and establishes its pathological relationship with pancreatic impairment and dysfunction and underscores to utilize AI techniques more successfully in the future for evaluating IBT.
研究领域:
微生物学
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糖尿病研究
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分子生物学
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人工智能
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免疫学
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代谢性疾病
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
An Yueqi,Ruan Qiufeng,Wenrong Li,Zhang Xuezhen,Xiong Shanbai
Understanding the differences in odors of freshwater and marine fish surimi and their products is important for the quality control of surimi products. Aroma compounds in silver carp surimi and three kinds of marine fish surimi and their products were identified by gas chromatography-mass spectrometry/olfactometry, and aroma fingerprints of them were established based on metabolomics analysis methods. Silver carp surimi and surimi products showed the highest “fresh fish” and “grassy, earthy” notes, while the marine fish surimi and their products presented a strong “sea breeze-like” odor. Five Br-containing compounds (sea breeze-like) were identified in the marine fish samples. The aroma fingerprints showed that the odor compositions of freshwater and marine surimi/surimi products were divided into two categories, and the marine fish surimi and their products also showed differences in odors. Furthermore, four commercial surimi and their products could be distinguished according to 33 and 28 differential aroma components, respectively.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Longxia Lan,Jinfeng Chen,Yuemei Chen,Jiajia Han,Yang Yu,Shaohan Wang,Guosheng Chen,Junlang Qiu,Fang Zhu,Gangfeng Ouyang
The wide present of phenicol antibiotics (PABs) in aquatic environments poses threats to aquaculture. Although PABs-induced adverse effects in fish have been reported, their influence on endogenous glucocorticoids (GCs) remain unknown. Herein, we developed an in vivo solid-phase microextraction (SPME) method to simultaneously monitor PABs and GCs in fish, thereby assessing the disturbance of GCs by PABs. By coupling to LC-MS/MS analysis, the method achieved great sensitivity (method detection limits ranging 0.0061–0.12 ng·g −1 ) and good reproducibility (RSDs of intra-/inter-fibers ＜10 %). The in vivo monitoring application showed PABs rapidly accumulated in fish with bioconcentration factors ranging 0.12–0.40. During exposure, the GC levels in fish were significantly elevated, in which cortisol (COL) consistently increased while corticosterone (COE) dropped to normal level after four days. PABs exposure induced elevated GC level, which subsequently enhanced the antioxidant defense system, leading to the mitigation of PABs-induced oxidative stress. During elimination, PABs were excreted and GCs, including COL and COE, returned to normal levels in only one day, with the stress responses and defenses subsided. These findings revealed the dual roles of GCs as stress indicators and adaptive regulators in fish under PAB exposure, and provided scientific support for developing aquaculture strategies in PABs contaminated waters. This study pioneered in in vivo simultaneous monitoring of exogenous and endogenous substances, facilitating future ecotoxicological investigations of emerging contaminants.
研究领域:
生态毒理学
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环境化学
·
水生毒理学
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分析化学
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Jin-Xiu Xu,Xin Zhang,Bai-Feng Fu,Zhao-Rui Li,Min-Bo Li,Zhen-Yu Wang,Xian-Bing Xu,Shu-Zhen Cheng,Ming Du
The traditional “bask in daytime and dewed at night” process is essential for fish sauce flavor development; however, the precise role of sunlight in aroma formation remains unclear. In this study, fish sauce mash (FM) was prepared using insulation fermentation with koji inoculation. Two batches were subsequently fermented for 30 days under two conditions: a sunlight-treated group (ST) and a darkness-treated group (DT). Electronic nose (E-nose) analysis revealed that the ST sample exhibited stronger responses to W1W (sulfur-organic compounds) and W5S (broadrange compounds) than DT and FM. A total of 90 flavor compounds were identified. Sunlight significantly promoted the formation of 8 alcohols, 5 aldehydes, 7 oxygen heterocyclic compounds, and 4 other classes of compounds. Based on relative odor activity values (ROAV ≥1), 24 key odorants were identified, among which 3-methylbutanal (ROAV: 97.19–336.26), 2-ethylfuran (ROAV: 35.71–82.64), and 2-methylbutanal (ROAV: 32.04–71.70) were the dominant contributors. The metabolic pathways of key odorants were predominantly associated with amino acid catabolism and fatty acid oxidation. Sensory evaluation indicated that sunlight exposure shifted the fish sauce aroma from predominantly meaty (FM) to a more complex and multilayered profile, intensifying roast, fishy, caramel, fermented, sour, ammonia, and rancid notes. Seven key odorants, including 3-methylbutanol, contributed significantly to this transformation. This study elucidates the regulatory role of sunlight in aroma formation during fish sauce fermentation and provides a theoretical basis for improving flavor quality and modernizing traditional fermentation practices.
研究领域:
食品科学
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发酵工程
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风味化学
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食品生物技术
·
代谢途径分析
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高影响因子
Membranes
·
2021
·Vol. 11
·期 10
·737
·
IF 4.106
Guoping Cheng,Liang Chen,Huanhuan Feng,Bo Jiang,Yi Ding
To construct a novel artificial cornea biomaterial, a method to prepare collagen lamellar matrix was developed in this study using grass carp scales as raw materials. The relationship between the structure of fish scale collagen lamellar matrix and the optical and mechanical properties was analyzed, and co-culture of it and rat bone marrow mesenchymal stem cells (BMSCs) was performed to preliminarily analyze the cellular compatibility of fish scale collagen lamellar matrix. The results show that the grass carp scales could be divided into base region, lateral region and parietal region according to the surface morphology. The inorganic calcium in the surface layer could be effectively removed by decalcification, and the decalcification rate could reach 99%. After etching treatment, homogeneous collagen lamellar matrix could be obtained. With the decalcification and etching treatment, the water content of the sample increased gradually, but the cross-linking treatment had no obvious effect on the water content of fish scale collagen lamellar matrix. Fish scale collagen lamellar matrix has good transparency, refractive index, mechanical properties and cellular compatibility, which may represent a prospect for the construction of cornea tissue engineering products.Keywords:corneal stroma;fish scale;collagen lamellar matrix;cell compatibility
研究领域:
生物材料
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组织工程
·
眼科学
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DOI
PubMed
高影响因子
Scientific Reports
·
2026
·
IF 4.9
Pan Ling,Tao Yubo,Li Peng
Humidity-driven smart materials have attracted increasing attention due to their capability of autonomous actuation without external energy input, offering significant potential in soft robotics and adaptive systems. However, natural polymer-based actuators often suffer from insufficient mechanical strength and limited structural stability, which restrict their practical applications. In this study, a novel biomimetic water-driven actuator was developed based on silane-modified microcrystalline cellulose (KMCC) and fish bladder gelatin (FG). A bilayer anisotropic structure inspired by natural hygroscopic systems was designed, and the effects of KMCC content and structural parameters on deformation behavior were systematically investigated through experimental characterization and finite element simulation. The results demonstrate that the incorporation of KMCC significantly enhances the mechanical and thermal properties of the composite hydrogel. The tensile strength increased from 0.258 MPa (pure FG) to 0.477 MPa at 10% KMCC, while the thermal decomposition temperature improved by approximately 11.81 °C. The actuator exhibited excellent reversible deformation behavior, with a maximum bending curvature of 0.083 at 10% KMCC content. Increasing KMCC content led to reduced deformation amplitude but improved structural stability. Furthermore, the actuator achieved controllable twisting deformation, with a maximum torsion angle of 2.04π and deflection of 66.93 mm at a 20° layer angle. Finite element simulation results were in good agreement with experimental data, confirming the reliability of the deformation mechanism. he deformation mechanism is governed by moisture diffusion and hydrogen bond reconstruction, which induce anisotropic volume changes and interfacial stress mismatch in the asymmetrical structure. This study provides a feasible strategy for enhancing the performance of water-driven actuators through material interface modification and structural design, offering new insights for the development of high-performance smart materials in soft robotics and adaptive systems.
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高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2025
·Vol. 41
·期 2
·358-366
·
IF 3
Dong Jiaxin,Su Rui,Wu Xiaokang,Dong Deming,Wang Yining,Huang Chenni,Qiu Tao,Gu Yu,Hua Xiuyi,Liang Dapeng
The study of the metabolic toxicity of perfluorooctanoic acid (PFOA) is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment, and provides a fundamental scientific basis for environmental protection and sustainable development. In this study, an electrospray ionization (ESI) source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid (PFOA) and their correlations in skin mucus and liver tissue of tilapia. Seven common metabolites were found in mucus and liver, and nitrogen metabolism pathways were disturbed in all of them. The up-regulation of glutamate in mucus may reflect an increased demand for amino acids and energy, whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism, protein synthesis and amino acid metabolism. Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways, which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.
研究领域:
环境科学
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毒理学
·
代谢组学
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高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 18
·4400-4407
·
IF 5.256
Mingtong Li,Hui Zhang,Mei Liu,Bin Dong
In this paper, we report a fish-like enzymeless motor, which consists of a phenylboronic acid modified poly(N-isopropylacrylamide) hydrogel and a surfactant, i.e. sodium dodecyl sulfate. The motor is capable of moving autonomously upon exposure to the glucose molecule. This phenomenon originates from the complexation between glucose and phenylboronic acid, which leads to the swelling of the hydrogel and the release of the surfactant, thus propelling the whole structure based on the Marangoni effect. Interestingly, the motor's moving velocity is directly proportional to the glucose concentration. Through the introduction of a magnetically responsive material, the motion direction of the motor can be manipulated. More interestingly, the combination of the glucose-dependent motion and magnetically steerable motion allows us to directly visualize the glucose concentration in human serum or urine. We thus believe that the motor reported in the current study may have potential for application in the field of glucose sensing for the detection and monitoring of diabetes.
研究领域:
生物医学工程
·
分析化学
·
材料科学
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DOI
高影响因子
ACS Applied Polymer Materials
·
2023
·
IF 5
Yanping Hao,Jiao Li,Chao Wang,Weikun Jiang,Xianhai Zeng,Chang Geun Yoo,Guihua Yang,Xingxiang Ji,Gaojin Lyu
The applications of conductive hydrogels in the fabrication of wearable sensors have been highlighted in recent studies. However, water in traditional hydrogels is volatile at room temperature and readily frozen at subzero temperatures, resulting in material failure, which is critical in their practical applicability. In this paper, we prepared an organogel with sodium lignin sulfonate (Ls) and poly(vinyl alcohol) in a dimethyl sulfoxide and water dual-solvent system and embedded dense fish scale biomimetic clusters of silver nanospheres on the surface of the organogel to impart high electrical conductivity and mechanical and antimicrobial properties. The obtained organogel exhibited excellent mechanical strength (1.58 MPa stress and 680% tensile strain), freeze resistance (−90 °C), and antimicrobial activity (bacteriostatic rate of more than 98%). In addition, the electrical conductivity of the organic gel still reached a high value of 1.9 mS cm–1 at the ultralow temperature of −90 °C. Moreover, the organogel sensor exhibited high strain sensitivity and fast response to various human movements; therefore, it accurately monitored human movement signals and physiological signals. This study proposes a simple method to prepare multifunctional lignin-based antifreeze organogels for their application in complex flexible wearable electronic devices, opening prospects for lignin-based antifreeze organogels.
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Environmental Science-Nano
·
2023
·
IF 7.3
Shuoli Ma,Wen-Xiong Wang
Nano-Zn (nZnO) has been increasingly applied in different fields, but the mechanisms of their uses as nutritional sources are less understood. Our study presents a regulatory mechanism of intestinal microbiota and structure mediated by nano-Zn, which subsequently improves the health and growth of fish. Fish diets were supplemented with 60 mg Zn kg−1 ZnSO4 (metal salt as the control group), 60 mg Zn kg−1 nZnO (powder), or 170 mg Zn kg−1 nZnO (powder) and then fed to golden pompanos for 5 weeks. We employed a new analytical amplicon sequence variant (ASV) method to identify the bacterial species and achieved the first in situ spatial quantification of fish tissue samples. Our results indicated that 60 mg Zn kg−1 of dietary nZnO promoted the probiotic colonization (Lactic-acid bacilli spp.) in the intestine. Upon exposure to a higher nZnO dose (170 mg Zn kg−1), Brevinema and Mycoplasma colonized in the fish intestine as pro-inflammatory microorganisms, which activated the C5 isoprenoid biosynthesis pathway and NAD biosynthesis. Furthermore, dietary nZnO (60 mg Zn kg−1) up-regulated the expression of genes related to intestinal mucus secretion and accelerated the goblet cell renewal in the intestinal villi. At a dose of 170 mg Zn kg−1 nZnO, the fish intestine significantly down-regulated occludin and mucin gene expression while displaying lower villus height, villus area, and goblet cell numbers, which ultimately led to abnormal digestion and absorption and immune functions. The Mantel test confirmed that the intestinal microbial abundance was significantly correlated with the changes in the intestinal structure, and the value of villi-to-crypt ratio decreased with the increase in Vibrio abundance, which was validated by the key genes and serum biochemical indexes. Finally, nZnO could regulate the glucose and lipid metabolism of fish by regulating microorganisms. Our results provided important insights into the interaction between nZnO and fish intestinal microbial community and host.
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高影响因子
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