CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Cong Chen,Boxin Zhang,Wenliang Wang,Jinbao Cheng,Bailiang Xue,Weikun Jiang,Lei Tian,Chengyuan Liang
TPO-S-CQD was fabricated from waste tires via a top-down approach. • TPO-S-CQD endows film with enhanced FRET-based fluorescence response. • The C3 film achieves a strong ΔE–TVB-N linear correlation (R 2 = 0.9945). • The C3 film possesses good antibacterial and physicochemical properties. Sulfur-doped carbon quantum dots (S-CQD) were synthesized via hydrothermal (bottom-up approach) or pyrolysis (top-down approach) methods, denoted as CA-S-CQD and TPO-S-CQD respectively. These were incorporated into cellulose substrate to construct fluorescent composite sensing films. Structural and spectral characterization revealed that, compared with CA-S-CQD, TPO-S-CQD possessed smaller particle size and superior dispersibility, enabling the formation of composite films with denser and more uniform microstructure. Based on the fluorescence resonance energy transfer (FRET) mechanism, the composite films exhibited more sensitive fluorescence responses to volatile biogenic amines. Specifically, the C3 film incorporating TPO-S-CQD demonstrated optimal performance in sensing sensitivity, recyclability, and long-term stability. Physicochemical properties tests indicated that the incorporation of S-CQD significantly enhanced the hydrophilicity, antioxidant activity, UV-blocking efficiency, and mechanical properties of the films, while maintaining excellent biodegradability. Moreover, the C3 film exhibited broad-spectrum antibacterial activity, with notably stronger inhibitory effects on E. coli than S. aureus . Using Litopenaeus vannamei as the detection object, the fluorescent color of the film changed gradually with spoilage degree at different storage temperatures, which was highly consistent with the national standard for total volatile basic nitrogen (TVB-N) in freshwater shrimp. The color difference (ΔE) showed a strong linear relationship with TVB-N content, with a coefficient of determination (R 2 ) of 0.9945 for the C3 film. This study realizes the high-value utilization of waste tires and provides a novel high-performance functional material and research strategy for intelligent indicator packaging and rapid visual freshness monitoring for freshwater shrimp. Download: Download high-res image (149KB) Download: Download full-size image
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高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2014
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IF 2.291
Wen-Sheng Zou,Fei-Hua Zou,Qun Shao,Jun Zhang,Ya-Qin Wang,Fa-Zhi Xie,Yi Ding
Fluorescein isothiocyanate (FITC) conjugated to polyethylenimine (PEI) (FITC&PEI) was synthesized and characterized. The nitrogen-containing organic groups played a key role in sensing nitroaromatic compounds that can bind 2,4,6-trinitrotoluene (TNT) to form TNT anions through an acid–base pairing interaction and FITC dye conjugation. The formation of TNT anions efficiently quenched the emission of FITC dye at 516 nm by fluorescent resonance energy transfer and induced the enhancement of a resonance light scattering (RLS) signal. A novel, dual-channel chemosensory FITC&PEI probe was thus constructed. Meanwhile, the FITC&PEI probe also exhibited highly selective fluorescence and RLS responses for TNT over other nitroaromatic compounds. Very good linearity was observed within the ranges of 3.0–150 nM TNT (detection limit down to 0.5 nM and RSD of 5.8%, n = 7) for fluorescence and 1.0–150 nM TNT (detection limit down to 0.2 nM and RSD of 8.4%, n = 7) for RLS. The proposed methods were well-suited for detecting and distinguishing trace amounts of TNT in aqueous solution.
研究领域:
分析化学
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环境科学
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高影响因子
Analytical Methods
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2019
·Vol. 11
·期 11
·1516-1521
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IF 2.378
Fujuan Luan,Zuhong Yu,Ling Yin,Xia Leng,Yuxue Shi,Jie Wang,Haibin Shi,Weichang Chen
Matrix metalloproteinase-2 (MMP-2) plays an important role in tumorigenesis and metastasis of gastric cancer. Therefore, specific and sensitive detection of MMP-2 is of great significance for clinical diagnosis and therapeutic evaluation of gastric cancer. Herein, we developed a new approach for accurate detection of MMP-2 activity both in gastric cancer cells and clinical tissues using a fluorescent probe Dab-PLGVRGY-FITC. The capability of the probe for detecting MMP-2 was systematically evaluated in gastric cancer MGC-803 cells and clinical gastric cancer tissues. Around 40-fold fluorescence enhancement at a wavelength of 520 nm was determined for the assays of the probe with recombinant MMP-2. The detection limit of MMP-2 was as low as 42 ng mL−1. Strong fluorescence signals were detected in MGC-803 cells and mouse gastric cancer tissues. Fluorescence signal enhancements of 11 clinical gastric cancer tissue lysates were 5.8 ∼ 9 times (7.53 ± 0.77 on average) higher than those from gastritis patients. This probe could be potentially used for early diagnosis of gastric cancer in clinics.
研究领域:
肿瘤学
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生物医学工程
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临床诊断
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高影响因子
Journal of Analytical Science and Technology
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2023
·Vol. 14
·期 1
·1-8
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IF 2.4
Xu Ting,Qingru Zeng,Fu Qing,Wang Zhaojie,Liu Xin,Xiao Shensheng,Jiang Xiaoming,Lu Yuepeng,Gong Zhiyong,Wu Yongning,Fang Min
Using amino silica modified with fluorescein isothiocyanate (FITC), a quick fluorescence analysis technique is used for the detection of 3-monochloropropane-1,2-diol (3-MCPD). At 100 °C and pH 8.5, FITC-modified amino silica exhibits the lowest fluorescence intensity in the presence of 3-MCPD. This can predominantly be explained mostly explained by 3-MCPD’s capacity to occupy the amino group that FITC normally binds to. The fluorescence intensity of FITC-modified amino silica was greatly quenched by 3-MCPD’s reaction with the amino group under alkaline conditions, and the fluorescence intensity is different at different reaction times, reaction pH, and reaction temperature. The effects of various 3-MCPD concentrations on the optical characteristics of FITC-modified amino silica were also investigated. Fluorescence analysis is used to obtain a linear range from 0.025 to 1.0 mg/L for 3-MCPD detection under optimal experimental conditions, with a detection limit of 0.025 mg/L and a correlation coefficient of 0.9915. The quantity of 3-MCPD in soy sauce was measured under ideal conditions. Using the optimized conditions, the contents of 3-MCPD in soy sauce were determined. These results suggest that this method is sensitive to 3-MCPD and may have a substantial application in the rapid detection of food contaminants particularly, where the quality and safety of food products are of paramount concern.
研究领域:
分析化学
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食品科学
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纳米技术
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Shuo Yang,Qijun Ding,You Li,Wenjia Han
Food safety is related to public health and environmental safety. Therefore, it is necessary to develop accurate and effective detection methods to assess food quality and safety. In this study, a pH-responsive functional film (BC/GA/FITC/PCA) was generated for the real-time and visual monitoring of shrimp freshness. Bacterial cellulose /Gelatin (BC/GA) was used as a film-forming matrix, and fluorescein isothiocyanate (FITC) and red cabbage (PCA) were used as the response signals. The addition of FITC and PCA increased the shading capacity (< 30 %) and antioxidant properties (22.8 %) of the films. WCA (82.73 ± 0.95°), WVP (1.48 × 10 −11 g·cm/cm 2 ·s·Pa) and OTR (2.42 × 10 −15 cm 3 ·cm/cm 2 ·s·Pa) indicated that the film possessed water resistance and oxygen barrier properties. When exposed to daylight, the film underwent a color transition from purple to green as the ammonia concentration increased. In addition, the blue-green fluorescence of the films gradually increased and the detection limit was low (170 ppb). In particular, the change in film color caused by shrimp spoilage corresponded to the TVBN value. This study work provides a new strategy for controlling and monitoring food safety and has a wide range of applications in the fields of food-active packaging and smart packaging.
研究领域:
食品科学
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生物材料学
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DOI
PubMed
高影响因子
BIOMATERIALS
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2015
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IF 8.557
Lei Zhang,Zhanzhuang Zeng,Chaohua Hu,Susan L. Bellis,Wendi Yang,Yintao Su,Xinyan Zhang,Yunkun Wu
Conventional oral vaccines with simple architecture face barriers with regard to stimulating effective immunity. Here we describe oral vaccines with an intelligent phase-transitional shielding layer, poly[(methyl methacrylate)-co-(methyl acrylate)-co-(methacrylic acid)]-poly( d , l -lactide-co-glycolide) (PMMMA-PLGA), which can protect antigens in the gastro-intestinal tract and achieve targeted vaccination in the large intestine. With the surface immunogenic protein (SIP) from group B Streptococcus (GBS) entrapped as the antigen, oral administration with PMMMA-PLGA (PTRBL)/Trx-SIP nanoparticles stimulated robust immunity in tilapia , an animal with a relatively simple immune system. The vaccine succeeded in protecting against Streptococcus agalactiae , a pathogen of worldwide importance that threatens human health and is transmitted in water with infected fish. After oral vaccination with PTRBL/Trx-SIP, tilapia produced enhanced levels of SIP specific antibodies and displayed durability of immune protection. 100% of the vaccinated tilapia were protected from GBS infection, whereas the control groups without vaccines or vaccinated with Trx-SIP only exhibited respective infection rates of 100% or >60% within the initial 5 months after primary vaccination. Experiments in vivo demonstrated that the recombinant antigen Trx-SIP labeled with FITC was localized in colon, spleen and kidney, which are critical sites for mounting an immune response. Our results revealed that, rather than the size of the nanoparticles, it is more likely that the negative charge repulsion produced by ionization of the carboxyl groups in PMMMA shielded the nanoparticles from uptake by small intestinal epithelial cells. This system resolves challenges arising from gastrointestinal damage to antigens, and more importantly, offers a new approach applicable for oral vaccination.
研究领域:
疫苗学
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免疫学
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水产养殖
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微生物学
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2023
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IF 6.2
Xin Qiao,Xiaoliang Wang,Haixu Chen,Yan Huang,Shangsong Li,Luxuan Li,Yinyong Sun,Xiaoman Liu,Xin Huang
Modulated membrane functionalization is a necessary and overarching step for hollow microcompartments toward their application as nanoreactors or artificial cells. In this study, we show a way to generate phospholipid hybrid proteinosomes that could show superposed virtues of liposomes and proteinosomes. In comparison to pure proteinosomes, both the membrane fluidity and permeability are improved obviously after forming the phospholipid hybrid proteinosomes. Specifically, the integration of phospholipids also endows the hybrid proteinosomes demonstrating a stepwise release of the encapsulants of FITC-dextran (70 and 150 kDa) triggered sequentially by phospholipase and protease, and then a modulated cascaded enzymatic reaction between two different populations of proteinosomes are achieved. Therefore, it is anticipated that such constructed phospholipid hybrid proteinosomes could be employed as an improved microcompartmental model for further advanced artificial cell design toward achieving logic signal communication within the various artificial cellular populations as well as potential applications in the field of microreactors.
研究领域:
纳米技术
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生物化学
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生物物理学
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细胞生物学
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化学工程
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DOI
高影响因子
Food & Function
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2022
·Vol. 13
·期 7
·4130-4141
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IF 6.317
Yao Guo,Yun-yun Shao,Yi-nan Zhao,Xiao Zhang,Zhuang-peng Chang,Yi-fan Sun,Jun-jin Liu,Jianping Gao,Rui-gang Hou
The aim of this work has been to establish and validate a simple and efficient method to detect the concentration of inulin-type fructan CPA from the roots of Codonopsis pilosula (Franch.) Nannf. in biosamples, and then apply it to evaluate the pharmacokinetics behavior, distribution character in tissue and excretion in mice. In this work, fluorescein isothiocyanate (FITC) was used to label CPA. Then FCPA was intravenously and orally administered to mice at different doses. In both i.v and p.o administration, FCPA concentration slowly declined in the circulatory system with a much longer T1/2 and MRT. After p.o administration, the area under the time curve (AUC0-∞) was dose-dependently increased. Taken together, FCPA showed poor absorption and wide tissue distribution. These pharmacokinetic results yield helpful insights into the pharmacological actions of FCPA.
研究领域:
药理学
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生物化学
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药剂学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2020
·Vol. 8
·期 15
·3016-3027
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IF 5.344
Qian Wu,Kaipeng Wang,Xichao Wang,Guohai Liang,Jinming Li
Controlling and detecting cell differentiation in human mesenchymal stem cells (hMSCs) for regenerative medicine remain challenging at present. Here, we developed multifunctional gold nanoparticles (AuNPs) to control and detect osteogenic differentiation in human mesenchymal stem cells (hMSCs) in real-time. The polyethyleneimine (PEI) capped AuNPs (Au-PEI) were synthesized first and were then conjugated with a matrix metalloproteinase 13 (MMP13)-sensitive peptide-FITC group (EGPLGVRGKG-FITC) to obtain multifunctional AuNPs (AuNP-PEI-peptide-FITC). AuNP-PEI-peptide-FITC could bind siRNA with PEI by electrostatic interactions to form the AuNP-PEI-peptide-FITC/siRNA nanocomplexes, allowing efficient siRNA delivery in hMSCs. The adipogenic-related gene peroxisome proliferator-activated receptor γ (PPARγ) was targeted for silencing by the AuNP-PEI-peptide-FITC/siRNA nanocomplexes to control osteogenic differentiation in hMSCs. After demonstrating that the AuNP nanocomplexes could control cell differentiation, the versatility of this tool was illustrated by showing that it can be used as a nanoprobe for real time detection of the osteogenic differentiation of hMSCs. This was done by measuring the activity of the MMP13 enzyme (produced during osteogenic differentiation) through the recovery of FITC fluorescence. This multifunctional AuNP showed a robust new methodology for controlling cell fate and simultaneously detecting cell differentiation in real-time for hMSCs, which is promising for multiple applications in regenerative medicine.
研究领域:
纳米技术
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干细胞生物学
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再生医学
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分子生物学
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DOI
PubMed
高影响因子
Oncotarget
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2016
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IF 5.008
Jianwen Zhao,Fengfeng Zhao,Xiyong Wang,Xiaobo Fan,Guoqiu Wu
A novel multifunctional nano-drug delivery system based on reversal of peptide charge was successfully developed for anticancer drug delivery and imaging. Mesoporous silica nano-particles (MSN) ~50 nm in diameter were chosen as the drug reservoirs, and their surfaces were modified with HIV-1 transactivator peptide-fluorescein isothiocyanate (TAT-FITC) and YSA-BHQ1. The short TAT peptide labeled with FITC was used to facilitate intranuclear delivery, while the YSA peptide tagged with the BHQ1 quencher group was used to specifically bind to the tumor EphA2 membrane receptor. Citraconic anhydride (Cit) was used to invert the charge of the TAT peptide in neutral or weak alkaline conditions so that the positively charged YSA peptide could combine with the TAT peptide through electrostatic attraction. The FITC fluorescence was quenched by the spatial approach of BHQ1 after the two peptides bound to each other. However, the Cit-amino bond was unstable in the acidic atmosphere, so the positive charge of the TAT peptide was restored and the positively charged YSA moiety was repelled. The FITC fluorescence was recovered after the YSA-BHQ1 moiety was removed, and the TAT peptide led the nano-particles into the nucleolus. This nano-drug delivery system was stable at physiological pH, rapidly released the drug in acidic buffer, and was easily taken up by MCF-7 cells. Compared with free doxorubicin hydrochloride at an equal concentration, this modified MSN loaded with doxorubicin molecules had an equivalent inhibitory effect on MCF-7 cells. This nano-drug delivery system is thus a promising method for simultaneous cancer diagnosis and therapy.
研究领域:
纳米技术
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药学
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癌症研究
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生物医学工程
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DOI
PubMed
高影响因子
MICROVASCULAR RESEARCH
·
2021
·
IF 3.514
Keke Ge,Yatao Wang,Pan Li,Miao Li,Wenhua Zhang,Handong Dan,Xumeng Hu,Jiamu Zhou,Qixiang Yang,Jiaojiao Wang,Zongming Song
The investigation aimed to evaluate the effects of Mcc950, an inhibitor of the NLRP3 inflammasome , on diabetic retinopathy (DR) mice. The general physiological condition of each group of mice was recorded. Retinal blood vessels were stained for observation of the density of blood vessels, and retinas were used for further morphological examination and fluorescent staining after the intravitreal injection of Mcc950. Mcc950 partially reversed hyperglycemia-induced vascular damage and had reduced histological changes compared to DR mice. IL-1β production in mice retinas in the diabetic model (DM) group increased, but pretreatment with Mcc950 significantly reversed these changes. Additionally, Mcc950 engineered reduced FITC dextran extravasation and vascular leakage. Therefore, it played an apparent protective role in DR and could be a new treatment strategy for DR.
研究领域:
医学
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眼科
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免疫学
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药理学
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DOI
PubMed
高影响因子
Future Medicinal Chemistry
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2024
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IF 3.2
Haili Yang,Xiaoyang Zhang,Letian Xu,Yuting Zhou,Rui Ma,Hao Chen,Siqin Zhao,Munkhtsetseg Baatar,Lvyi Chen,Xukun Deng,Hongwei Gu,Xiaoming Wang
Aim: Lysosomal pH changes are associated with drug resistance, cell growth and invasion of tumors, but effective and specific real-time monitoring of lysosomal pH compounds for cancer therapy is lacking. Materials & methods: Here, based on the covalent linkage of the anticancer drug palbociclib and fluorescent dye fluorescein isothiocyanate (FITC), we designed and developed a novel palbociclib-derived multifunctional molecule (Pal-FITC) for lysosomal targeting and diagnostic therapeutic integration. Results & discussion: Pal-FITC fluoresces is 20-fold stronger than that of FITC and shows a linear response in the pH range of 4.0–8.2 (R2 = 0.9901). Pal-FITC blocks cells in G1 phase via Cyclin D-CDK4/6-Rb. Conclusion: Our study provides new strategies for tumor-targeted imaging and personalized therapy.
研究领域:
肿瘤学
·
药物化学
·
分子生物学
·
诊疗一体化
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）