INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 24
·期 1
·458
·
IF 6.208
Xiuming Li,Qifang Wang,Haosen Li,Xiaoyun Wang,Ruimin Zhang,Xiaoyu Yang,Qiwei Jiang,Qinghua Shi
Fusarium oxysporum f.sp.radicis-lycopersici(Forl) is a destructive soil-borne phytopathogenic fungus that causes Fusarium crown and root rot (FCRR) of tomato, leading to considerable field yield losses. In this study, we explored the antifungal capability of linalool, a natural plant volatile organic component, againstForland its role in controlling FCRR symptoms in tomatoes. Our results showed thatForlmycelial growth was inhibited by the linalool treatment and that the linalool treatment damaged cell membrane integrity, enhanced reactive oxygen species levels, depleted glutathione, and reduced the activities of many antioxidant enzymes inForl. Transcriptomic and proteomic analyses demonstrated that linalool also downregulated metabolic biosynthetic pathways at the transcript and protein levels, including redox, transporter activity, and carbohydrate metabolism inForl. Moreover, linalool significantly decreased the expression of manyForlpathogenic genes, such as cell wall degrading enzymes (CWDEs) and G proteins, which is likely how aForlinfection was prevented. Importantly, exogenously applied linalool activated the salicylic acid (SA) and jasmonic acid (JA) defensive pathways to improve disease resistance and relieved the negative effects ofForlon plant growth. Taken together, we report that linalool is an effective fungicide againstForland will be a promising green chemical agent for controlling FCRR.Keywords:volatile compounds;antifungal mechanism;Fusarium oxysporum f.sp.radicis-lycopersici;Fusarium crown and root rot;transcriptomic and proteomic
研究领域:
植物病理学
·
分子生物学
·
农业科学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2014
·
IF 2.61
Lingchen Yang,Zhiyong Zhao,Yifeng Deng,Zhenlei Zhou,Jiafa Hou
The T-2 and HT-2 toxins, the main metabolites of Fusarium poae , induce toxicity in broilers and accumulate in tissues. Consequently, during the breeding process of broilers, diets are frequently supplemented with physical adsorbents to protect birds against the toxicity induced by mycotoxins. In the present research, T-2 and HT-2 were produced in maize inoculated with F. poae . Mont, the strongest adsorbent based on in vitro adsorption ratios, was added to the contaminated diet. One-day-old chickens were randomly and equally divided into the following four groups: control diet group, Mont supplemented diet group, contaminated diet group and detoxification diet group. The experiment lasted for 42 days. Compared to the control group, the contaminated group showed significant decrease in body weight, feed intake and TP ( P < 0.05), and marked increase in FCR, ALP, AST and ALT activity, T-2/HT-2 residues in the tissues and the relative expressions of apoptosis-related mRNAs ( P < 0.05). Mont supplementation provided protection for the treated broilers in terms of performance, blood biochemistry, hepatic function, T-2/HT-2 residue of tissues and apoptosis. Therefore, Mont may be suitable as a detoxification agent for T-2/HT-2 in feed for broilers.
研究领域:
动物科学
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毒理学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yibo Liu,Yuee Tian,Hong Peng,Fulong Zhang,Xiaobo Huang,Genqiang Chen,Lin Zhou,Zhiping Che
Paeonol is the main active ingredient in the Chinese medicinal herb peony root bark. To discover biorational natural product-based fungicides, 25 paeonol derivatives (5a–y) containing a pyrazole ring were designed and synthesized by ingeniously introducing a pyrazole five-membered heterocyclic ring, and their structures were well characterized by 1H NMR, 13C NMR, high-resolution mass spectrometer (HRMS), and m.p. The stereochemical configurations of six target compounds, 5c, 5f, 5g, 5o, 5s, and 5v, were unambiguously subjected to single-crystal X-ray diffraction. Furthermore, we evaluated the bioactivities of these target compounds as anti-oomycete and antifungal agents against two serious agricultural diseases, Phytophthora capsici and Fusarium graminearum. Among all tested compounds, (1) nine compounds 5b–d, 5f, 5g, 5r, 5s, 5u, 5w displayed promising anti-oomycete against P. capsici, with EC50 values ranging from 22.1 to 34.5 mg/L, and the protective effect of the target compounds against P. capsici in vivo further confirmed the above results. (2) Six compounds 5g, 5k, 5m, 5t, 5w, 5x displayed promising antifungal against F. graminearum, with EC50 values ranging from 38.2 to 86.0 mg/L. Moreover, some interesting results of structure–activity relationships were also observed. The results of this study pave the way for further design, synthesis, and development of paeonol derivatives as potential botanical anti-oomycete and antifungal agents in crop protection.
研究领域:
植物化学
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农药科学
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGICAL METHODS
·
2018
·
IF 1.701
Yu-Ke Cen,Jian-Guang Lin,Jun-You Wang,Zhi-Qiang Liu,Yu-Guo Zheng
A colorimetric assay has been developed for quantitative analysis of active biomass of Fusarium fujikuroi , based on the reduction of the tetrazolium salt 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) when menadione was present as an electron coupling agent. The optimum assay-conditions were set as 200 μg/ml XTT, 5 μM menadione and one-hour reaction time. Under these settings, the produced formazan displayed a linear relationship with F. fujikuroi biomass. This method was subsequently applied to evaluate the cell growth behavior, which showed a positive correlation with the carbon source consumption and gibberellin biosynthesis under the industrial fermentation conditions. Our results showed that the XTT-menadione assay is a valuable tool in analyzing the industrial fermentation process of F. fujikuroi , especially when the medium contains insoluble and complex components.
研究领域:
微生物学
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生物技术
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真菌生物学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Jian Guo,Jiaoyu Wang,Hui Xie,Junlong Jiang,Chunyuan Li,Wanting Li,Ling Li,Xingquan Liu,Fucheng Lin
The continuous usage of fungicides poses a potential threat to the environment, ranging from mere irritation to being very toxic to human beings and organisms. Plasma-activated water (PAW) has recently gained much interest as a promising candidate to inactivate phytopathogens. However, the inactivation mechanisms of PAW are still not well understood. In this study, the effect of PAW on the cellular responses of Fusarium graminearum and the efficacy of PAW against F. graminearum in vivo and in vitro were investigated. The results showed that microbial activity of spores was significantly inhibited by PAW treatment ( P < 0.05 ) . The symptoms caused by F. graminearum were significantly reduced on the wheat spikelets. PAW could induce cell wall sculpturing, membrane permeability changes, and mitochondrial dysfunction. Differential gene expression analysis also confirmed that the cell membrane, the cell wall and the mitochondria were the organelles most affected by PAW. The results from this study facilitate the understanding of the mechanisms underlying the responses of F. graminearum to PAW and indicate the potential of PAW as a fungicidal agent or an effective supplement to fungicides.
研究领域:
植物病理学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
NATURAL PRODUCT RESEARCH
·
2024
·
IF 1.9
Xiaofang Zhang,Xiaolong Guo,Wanying Yin,Ruiguang Wang,Yuee Tian,Huilu Sun,Shaobin Xu,Shaoyan Shuang,Xiaobo Huang,Genqiang Chen,Zhiping Che
To discover biorational natural product-based pesticides, a series of paeonol ester derivatives containing a Schiff base (6a-j, 7i,j, 8i,j, and 9i,j) were prepared, and their structures were well characterised by 1H NMR and HRMS. Furthermore, bioactivities of these compounds as anti-oomycete and anti-fungal agents against two serious agricultural diseases, Phytophthora capsici and Fusarium graminearum we assessed. Amongst evaluated compounds, 1) Compounds 6a and 6e displayed good anti-oomycete against P. capsici, with EC50 values of 116.50 and 88.86 mg/L, respectively. 2) Compounds 6e and 7i exhibited prominent anti-fungal against F. graminearum, with EC50 values of 66.73 and 29.33 mg/L, respectively. 3) This study suggested that the introduction of nitro at the C5 position of paeonol could improve its bioactivity against P. capsici and F. graminearum. The results of this study pave the way for further design and development of paeonol derivatives as plant anti-oomycetes and anti-fungal agents in crop protection.
研究领域:
植物化学
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农药学
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植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Fengjiao Hao,Shuning Chen,Huizhu Yuan,Xiaojing Yan,Daibin Yang
The plant root is a key pathway to absorb insecticides from soil and is colonized by beneficial and pathogenic microbial communities. Our study demonstrated that colonizing roots by nitrogen-fixing bacterium Pseudomonas stutzeri and pathogenic Fusarium graminearum and Pythium ultimum increased the uptake of insecticides into maize roots from soil. An alteration in the permeability of root cells contributed to this increased uptake. For the subsequent root-to-shoot translocation, the relationship between translocation and log P of the compound satisfied a Gaussian distribution. Relatively beneficial P. stutzeri can promote maize seedling growth and increase translocation, whereas Fusarium and Pythium pathogens can retard the seedling growth and reduce the translocation. Furthermore, the relationship between the concentration difference (difference of an insecticide from inoculation treatment to control) and log P also showed a Gaussian distribution. The maximum concentration difference from the Gaussian equation can be applied to assess the capacity of rhizosphere microorganisms to influence translocation.
研究领域:
植物学
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环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 7
·1558
·
IF 4.6
Dingtao Tang,Jiajie Quan,Zhengjiao Gao,Bingfeng He,Yu Hou,Peipei Fan,Meidong Pan,Jiali Yang
Prenylation increases the structural diversity and biological activity of flavonoids. In this study, an aromatic prenyltransferase, FgPT1, was identified fromFusarium globosum. This enzyme was demonstrated to specifically catalyze the prenylation of flavanones, including naringenin, hesperitin, eriodictyol, liquiritigenin, rac-pinocembrin, and dihydrogenistein, and exhibited no activity toward other types of flavonoids, including chalcones, flavonols, isoflavonoids, and flavonols. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and nuclear magnetic resonance (NMR) analysis indicated that the majority of prenylated products were 6-Cprenyl flavanones, with the exception of liquiritigenin, which was additionally transformed to 4′-Oprenyl liquiritigenin. Enzyme kinetic analysis suggested that FgPT1 exhibited the highest catalytic efficiency towards naringenin, with akcat/KMvalue determined as 61.92 s−1M−1, and the lowest catalytic efficiency towards liquiritigenin, with akcat/KMof 1.18 s−1M−1. Biochemical characterization suggested that FgPT1 functioned as a metal-dependent enzyme with optimal activity in the presence of Ba2+at pH 7.5 and 30 °C. Site-directed mutagenesis resulted in a series of mutants, including A325V with impaired prenylation activity and V116I, V181I, and V194I with enhanced activity. V194I displayed the highest enzymatic activity with a nine-fold increase compared to wild-type FgPT1.
研究领域:
生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plant Nano Biology
·
2026
·
IF 9.1
He Meng,Wenke Wang,Yan Fang,Caiguo Tang,Yan Duan,Qin Zhai,Chenxu Zhou,Huilan Zhang,Lifang Wu
Fusarium graminearum (F. graminearum)- induced wheat Fusarium head blight (FHB) substantially reduced wheat yield and grain quality, and has evolved into a serious threat on a global scale. Although chemical pesticides served as the mainstay for FHB prevention for decades, prolonged use has led to pathogen resistance and environmental risks, which in turn constrain sustainable development. Biogenic nanomaterials have attracted global attention for their potential to substitute for partial pesticides due to their environmentally friendly and highly effective properties, such as biogenic selenium nanoparticles (Bio-SeNPs). However, the understanding of the underlying mechanisms of Bio-SeNPs in preventing and controlling FHB is still rudimentary. To address it, this study carried out both in vivo and in vitro experiments, as well as field trials to verify the function of Bio-SeNPs. Therefore, Bio-SeNPs were synthesized by Bacillus subtilis 168. In vitro assays demonstrated that 0.4 mmol L⁻¹ was the optimal concentration of Bio-SeNPs for inhibiting F. graminearum growth, achieving an inhibition efficiency of over 70%. In vivo experiment showed that Bio-SeNPs (0.4 mmol L⁻¹) not only significantly alleviated F. graminearum -caused browning in wheat seedlings and spikes, but also promoted proline accumulation, enhanced antioxidant enzyme activities, while reducing reactive oxygen species accumulation. In field trials, Bio-SeNPs application decreased FHB disease index by more than 80%, further demonstrating their role in plant protection. Summarily, the application of Bio-SeNPs can restrain the occurrence of FHB by directly inhibiting the pathogens' growth, indirectly enhancing systemic resistance of wheat, thus delivering nove l insights and viable strategies for sustainable management of FHB.
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DOI
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 43
·e202201546
·
IF 2.307
Yue Ma,Rui Zhou,Xiong-Fei Luo,An-Ping Li,Rui Wang,Bao-Qi Zhang,Han Zhou,Tian-Lin Wu,Yi-Rong Wang,Jun-Xia An,Zhi-Jun Zhang,Wen-Bin Zhao,Cheng-Jie Yang,Yan Yan Ding,Ying-Qian Liu
Graphical Twenty-one phenolic compounds such as thymol and carvacrol were selected as the research objects to screen the antifungal activities against 6 phytopathogenic fungi. The best antifungal compound Z-5 and the most sensitive strain ( Fusarium graminearum ) were selected as further research objects. The mechanism was preliminarily discussed from the aspects of cell membrane destruction, oxidative stress, deoxynivalenol content, etc., which provided new ideas for the prevention and treatment of Fusarium graminearum . Phytopathogenic fungi infections have become a major threat to reduce crop yields and dampen human health globally. The mycelium growth inhibition method was adopted to evaluate the antifungal activity of commercially available natural and natural-like phenols, bioassay results showed that most phenolic compounds exhibited excellent fungicidal activity against Fusarium graminearum , Rhizoctonia solani , Botrytis cinerea , Fusarium oxysporum f. sp . Vasinfectum, Sclerotinia sclerotiorum , and Magnaporthe oryzae . Particularly, compound Z-5 exhibited an excellent inhibition rate of 100.00 % at 100 μg/mL and EC 50 value of 3.20 μg/mL against Fusarium graminearum. In addition, compound Z-5 displayed the best efficacy and superior fungicidal activity compared to azoxystrobin (EC 50 =9.78 μg/mL) based on mycelial growth and spore germination. Significantly, compound Z-5 at 200 μg/mL reduced 4-deoxynivalenol production by over 98 % compared to the control, through down-regulation of the gene expression of Tri5 and Tri6. Preliminary mechanism studies revealed that compound Z-5 might exert its fungicidal activity by inducing accumulation of reactive oxygen species and lipid peroxidation, and causing cell membrane destruction and DNA synthesis interference. This study demonstrated the potent fungicidal activity of the natural-like phenolic compound Z-5 and highlighted its potential as an alternative agent to control F. graminearum .
研究领域:
植物病理学
·
真菌学
·
植物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
Microorganisms
·
2026
·Vol. 14
·期 2
·322
·
IF 4.2
Yifan Chen,Li Zhang,Yan Chen,Wenpei Ge,Hongling Wang,Zhanying Xue,Xinran Cui,Xin Wang,Aimei Liao,Yuansen Hu,Na Liu
Fusarium solani , a widely distributed plant pathogenic fungus, poses a significant threat to various crops due to its complex pathogenic mechanisms and being difficult to control. In this study, LC–TOF–MS analysis identified 4-(4-hydroxyphenyl)-2-butanone (RK) as a metabolite detected in Bacillus amyloliquefaciens strain M1, and a commercially available RK standard was subsequently used to evaluate its antifungal activity against F. solani . Antifungal assays demonstrated that RK effectively suppressed fungal growth. Further physiological and biochemical assays confirmed that RK disrupts the cell membrane and mitochondrial function, leading to intracellular reactive oxygen species (ROS) accumulation. This study offers new perspectives on the antifungal mechanism of RK and offers theoretical support for the development of innovative agricultural disease management strategies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Zang Yuan-Yuan,Yang Sha,Xu Yong-Qiang,Chen Zhi-Gang,Wu Tao
In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 – 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40oC with the degradation rate of 10.65 mg min−1 L−1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production.
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DOI
PubMed
高影响因子
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