Analytical Methods
·
2019
·Vol. 12
·期 1
·91-96
·
IF 2.378
Peiyao Lv,Ying Cao,Zi Liu,Rong Wang,Baoxian Ye,Gaiping Li
In this work, we report a new fluorescence ratiometric method for Hg2+ assay based on the dual-ligand fluorescent probe GMP–Tb–luminol coordination polymers (CPs), which can be excited at the same wavelength and reveal characteristic luminescence of Tb3+ and luminol with spectrally distinguishable emission peaks. The addition of Hg2+ leads to the decrease of Tb3+ luminescence due to the higher coordination between Hg2+ and GMP, which inhibits energy transfer from GMP to Tb3+. Meanwhile, the fluorescence of luminol increases because of the aggregation-induced emission phenomenon. Therefore, a ratiometric fluorescent sensor for Hg2+ assay can be constructed by measuring the ratio of fluorescence intensity of luminol at 430 nm (I430) to that of Tb3+ at 548 nm (I548). Under the optimal conditions, the fluorescence intensity ratio I430/I548 exhibits a good linear relationship versus the concentration of Hg2+ in the range from 5 nM to 130 μM with a low detection limit of 1.3 nM. Additionally, the probe can also adsorb Hg2+ in water with high efficiency, which makes Hg2+ removal in water possible. The proposed method has been successfully used for the determination of Hg2+ in tap water, indicating its potential applications in the detection of Hg2+ pollution in environmental water.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Theranostics
·
2022
·
IF 11.6
Xiaotong Yu,Jing Yu,Hong Dai,Chenyun Deng,Xudong Sun,Sijie Long,Zhujun Jiang,Hongyan Jin,Zhu Guan,Zhenjun Yang
Rationale: Cyclic dinucleotides (cDNs) are a promising class of immunotherapeutic agent targeting stimulator of interferon genes (STING). However, enzymatic instability and transmembrane barriers limit the extensive clinical application of cDNs. Thus, a novel delivery system, composed of a neutral cytidinyl lipid DNCA and a cationic lipid CLD (Mix) that interacts with cDNs via H-bonding, pi-stacking and electrostatic interaction, is developed and optimized to overcome the above issues. Methods: The optimal composition of Mix for cDNs encapsulation was explored with RAW-Lucia ISG cells. The physicochemical properties of resulted nanoparticles were characterized. To validate the anti-tumor immunity of cDNs/Mix both in vitro and in vivo , immunogenic cell death (ICD) related markers and tumor inhibition efficacy were evaluated in cancer cells and tumor models, respectively. The mechanism by which cdG/Mix exerted the antitumor effects was explored by flow cytometric analysis and in vivo depletion. Results: Based on our developed and optimized delivery system, neutral cytidinyl lipid DNCA/cationic lipid CLD (Mix), cdG (500 nM in vitro , 1-10 μg in vivo )/Mix not only more potently stimulated production of IFNβ and related cytokines including CXCL9 and CXCL10, promoted ICD, led to NK and CD8 + T cell activation, inhibited tumor growth in both EO771 and B16F10 models and increased their survival rate (~43%), but also obviously reversed the T cell exhaustion (Tex) in tumor, meanwhile down regulated the mRNA expression of Tox and Nr4a , which are key regulators of Tex. Conclusion: cdG/Mix triggered ICD in various cancer cells and reversed the Tex systemically in tumor-burden mice, which would be a promising alternative strategy for cancer immunotherapy.
研究领域:
免疫学
·
肿瘤学
·
药学
·
癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Yingying Yang,Jingyu Xu,Yanzhu Guo,Xing Wang,Ling-Ping Xiao,Jinghui Zhou
Laccase is a highly versatile multicopper oxidase for lignin biodegradation. Using nanomaterials to realize functional laccase simulations is attractive for applications and fundamental research. Herein, this study reports a facile strategy to fabricate a Cu metal–organic framework (MOF) by using copper as a metal center with guanosine 5′-monophosphate (GMP) as organic ligands (Cu/GMP). This obtained catalyst exhibits excellent chemoselectivity for lignin depolymerization into oligomers with low molecular weights at room temperature and atmospheric pressure. It was found that Cu/GMP shows superior laccase-like activity, which is beneficial for the C–O bond cleavage within lignin, thus affording oligomers in a high yield (81.7 wt %). Furthermore, the effects of the obtained lignin oligomers on the oxidative stability of canola oil biodiesel were evaluated. They were novel, renewable, and of low cost, which as biodiesel additives exhibited high potential in replacing expensive merchand biodiesel antioxidants commonly used to date.
研究领域:
生物化学
·
纳米生物学
·
可再生资源与环境
✓ 已验证
DOI
高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2020
·Vol. 32
·期 6
·1519-1529
·
IF 1.731
Soomro Najaf Ali,Amur Safdar Ali,Wei Yun,Shah Aamir Hassan,Jiao Mengzhao,Liang Hao,Yuan Qeping
Abstract Silver nanoparticles (AgNPs) are effective antibacterial agent but lack of stability and rapid aggregation limits their antibacterial efficiency in biomedical applications. To overcome these shortcomings, various strategies have been adopted so far but those strategies are not much effective at room temperature and in absence of external stimuli. Thus, herein, we have successfully grafted AgNPs into copper and guanosine 5′-monophosphate (Cu/GMP) metal organic frameworks at room temperature without using any external stimuli. The loading encapsulation ratio of AgNPs into Cu/GMP was approx. 99.83% at neutral pH. The AgNPs@Cu/GMP was characterized by various techniques, such as energy-dispersive x-ray spectroscopy, transmission electron microscopy, x-ray diffraction and Fourier transforms infrared spectroscopy. All these techniques have confirmed the loading of AgNPs into Cu/GMP (MOFs). Furthermore, antimicrobial activities of AgNPs, Cu/GMP and AgNPs@Cu/GMP were assessed against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacterium. As compared to pure AgNPs, the composite material AgNPs@Cu/GMP had shown significant growth of inhibition zones against tested bacteria. The stability of AgNPs@Cu/GMP was remarkable, i.e., six times higher than free AgNPs even after 15 days. These results make AgNPs encapsulated into Cu/GMP a promising antimicrobial candidate and is expected to deliver an advance platform to pharmaceuticals and biomedical applications. Graphic abstract
研究领域:
材料科学
·
微生物学
·
药学
✓ 已验证
DOI
高影响因子
Applied Biological Chemistry
·
2022
·Vol. 65
·期 1
·1-10
·
IF 3.206
Yu Ying,Huan Siqi,Wang Xiaodan,Yang Cong,Liu Dengyong
A sensitive electrochemical sensor for the selective detection of 5ʹ-guanylic acid (5ʹ-GMP) was prepared by combining sulfonated-multiwalled carbon nanotubes (SMWCNTs) and [Ru(bpy)2dpp]Cl2, which were dripped on the surface of a glass carbon electrode (GCE) immobilized with gold nanoparticles. The 5ʹ-GMP electrochemical biosensor was fabricated using [Ru(bpy)2dpp]2+/SMWCNTs/Au/GCE as working, Ag/AgCl as reference and Pt as auxiliary electrode connected by an electrochemical workstation. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed the sensor’s response current had the best peak shape and maximum peak when the pH of electrolyte was 3, scan speed of CV was in the range of 100 to 180 mV/s, and the enrichment time was in the range of 200 to 300 s. Under the optimum conditions, a linear analytical curve was obtained for 5ʹ-GMP concentrations in the range of 0.01 to 0.5 mmol L−1, with a detection limit of 0.0014 mmol L−1. The analytical results of the 5ʹ-GMP sensor were exhibited good consistent with the data from liquid chromatography. The sensor has good reproducibility, long-term stability and strong immunity to interference, and may be a powerful device for 5ʹ-GMP detection, with great advantages such as simple preparation and operation, low equipment cost.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
iScience
·
2022
·
IF 6.107
Shixun Liu,Anming Xu,Bin Xie,Fengxue Xin,Weiliang Dong,Jie Zhou,Min Jiang
Summary The synthesis of biofilm exopolysaccharides and rhamnolipids (RLs) are two interrelated processes in Pseudomonas aeruginosa , but how bacteria coordinate these two processes remains unclear. We collected a P. aeruginosa KT1115 with rugose small colony variant (RSCV) phenotype from soil, and used it to study the dynamic regulation mechanism of biofilm polysaccharide and RLs synthesis. The results showed that the overproduction of biofilm exopolysaccharides at biofilm stage ultimately contributed the surge of RLs production at RLs stage. This phenomenon was further verified by comparing PAO1 with its engineered RSCV mutant, PAO1Δ wspF . Further genomic, transcriptomic analyses and gene deletion revealed that downregulation of c-di-GMP level was the key to switch biofilm exopolysaccharides accumulation to RLs surge, by transcriptionally upregulating a c-di-GMP phosphodiesterase NbdA. Overall, this study demonstrates the importance of c-di-GMP in coordinating biofilm exopolysaccharides and RLs synthesis, and provides an inspiration for enhancing RLs production through regulating c-di-GMP level.
研究领域:
微生物学
·
分子生物学
·
细菌生理学
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2022
·
IF 7.613
D. Meng,Y. Li,P. Qi,J. Sun,X. Gu,H. Li,Z. Wang,S. Zhang
The fire hazard posed by the inherent flammability and smoke toxicity of flexible polyurethane foam (FPUF) often necessitates the incorporation of flame retardants to meet stringent regulations. The development of environmentally friendly strategies to enhance the fire resistance of FPUF is of increasing importance and a significant amount of research has been devoted to this end. In this work, a bio-based coating was constructed by co-depositing polydopamine and guanosine 5′-monophosphate disodium salt (GMP) to increase the fire safety of FPUF. The coating significantly improved the flame retardancy of FPUF. The FPUF@PDA-GMP samples showed an LOI value of 24.5% with a self-extinguishing effect and exhibited reduced release of heat, smoke, CO, CO 2 , and HCN due to their compact and strong char. The compressive strength at 70% strain of FPUF@PDA-GMP was 8 times higher than that of the control FPUF sample. The coating had a little negative effect on the resilience and improved its elongation at break. This investigation has provided a useful method for designing and producing high-performance foams.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Microbiology Spectrum
·
2026
·
IF 4.1
Xueqing Zhao,Yucheng Shi,Wenzhuo Tian,Yutong Tian,Chuxiao Hu,Ziye Guo,Ting Ma,Guoqiang Li,Ge Gao
Cyclic di-GMP (c-di-GMP) acts as a bacterial second messenger that regulates various physiological processes, including biofilm formation, motility, and virulence. It is synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs), with precise regulatory effects typically requiring the coordinated action of multiple DGCs and PDEs. Kosakonia oryzendophytica FY-07 (FY-07) exhibits high bacterial cellulose (BC) yield, rapid growth, and robustness and can produce BC under varying oxygen conditions; however, the underlying mechanism driving the phenotypic transition from cellulose-producing to motile states during fermentation has yet to be elucidated. In this study, we aimed to identify two DGCs, KoBdcA and KoBdcB, and one PDE, KoBpdA, whose encoding genes are located adjacent to the cellulose synthesis operon in FY-07. KoBdcA and KoBpdA form an antagonistic DGC-PDE pair that maintains intracellular c-di-GMP homeostasis. Deletion of either gene alters BC production and motility. In contrast, KoBdcB acts as an auxiliary component, and its deletion alone causes no phenotypic changes. KoBdcA regulates BC synthesis via an indirect interaction with BcsA mediated by KoBdcB. The Per-Arnt-Sim (PAS) domains of KoBdcA and KoBpdA bind hemin, enabling them to sense oxygen and modulate their enzymatic activities. Elevated DGC activity during early fermentation promotes BC production, while increased PDE activity later suppresses BC synthesis, shifting cells to a motile state.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 24
·7849-7857
·
IF 3.978
Chang Liu,Chunyu Huang,Ruixue Ma,Wanying Zhai,Jingjing Deng,Tianshu Zhou
This work demonstrates a new strategy for sensing cerebrospinal acetylcholinesterase (AChE) as a cholinergic biomarker for brain dysfunction based on graphene quantum dot (GQD)-functionalized lanthanide infinite coordination polymer (Ln-ICP) nanoparticles. The ICPs used in this work were comprised of two components, i.e. a supramolecular Ln-ICP host formed by the coordination between the GMP ligand and central metal ion Tb3+, and guest GQDs with abundant functional groups, which were utilized as antenna ligands to further sensitize the fluorescence of Tb/GMP. Upon excitation at 300 nm, the obtained GQD@Tb/GMP ICP nanoparticles exhibited enhanced green fluorescence from Tb/GMP. With the addition of Cu2+, the competitive coordination between Cu2+ and GQDs weakened the antenna effect, leading to a decrease in the fluorescence of GQD@Tb/GMP ICPs. However, in the presence of thiocholine (TCh), a thiol-containing compound hydrolyzed from acetylthiocholine (ATCh) by AChE, a stronger coordination interaction between Cu2+ and TCh occurred, resulting in the restoration of the fluorescence of GQD@Tb/GMP ICPs. Using the method established herein, the cerebrospinal AChE fluctuation of rats with acute organophosphorus pesticide (OP) poisoning or chronic Alzheimer's disease (AD) could be monitored. This study essentially provides a novel approach to realize the direct monitoring of a biomarker for brain dysfunction by regulating the competitive coordination interaction reversibly, which is critical in the early diagnosis and therapy of brain diseases.
研究领域:
纳米技术
·
生物化学
·
神经科学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Suyue Liu,Jingjing Tian,Longjiao Zhu,Hongtao Tian,Min Yang,Kunlun Huang,Wentao Xu
To develop a fast, isothermal, enzyme-free and visible method for the on-site detection of silver ions (Ag + ), a ratiometric Ag + sensor was constructed based on a dual-emission ternary nucleotide terbium probe, fabricated by synthesizing the luminescent nanomaterial Luminol-GMP-Tb (LGT). We repositioned the fluorescent properties of the nanomaterial from the perspective of the inherent optical properties of two signal reporters (luminol and Tb 3+ ) through highlighting the absorption, fluorescence steady-state measurement and time-resolved properties. Furthermore, based on the ingenious coordination interaction and energy transfer among Ag + , luminol and GMP-Tb, we hypothesized and confirmed that Ag + induced morphology and luminescence changes of LGT through scanning electron microscopy and lifetime changes as well as fluorescence changes. Meanwhile, by means of a portable ultraviolet detector with dual wavelengths, based on the fluorescence excitation characteristics of each component, different photoluminescence color phenomena presented at different excitation wavelengths. Therefore, visual qualitative detection could be realized under a portable UV lamp with 254 nm for mainly visible green color change while 365 nm as built-in reference for blue-emitting luminol. The ratio calculation method and the enhanced luminescence ratio mechanism made LGT exhibited extremely high selectivity to Ag + . Quantitative detection Ag + was realized in two ranges of 0.1 μM ~ 6 μM and 6 μM ~ 100 μM with a minimum detection limit of 65 nM. The method has been successfully exploited in real water samples with recovery rate between 99.7% and 106.38%, showing broad application prospects in food safety and environmental monitoring for the on-site detection of Ag + .
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Li Yang,Yonghai Song,Mulan Zeng,Yue Du,Bingxian Peng,Zhenzhong Huang,Li Wang
It is of great significance for superoxide anion (O 2 •− ) detection due to its important role in regulation of biological function. The nanoscale coordination polymers (NCPs) core-shell nanostructures have attracted increasing interests for their nanosized-dependent properties. In this study, SiO 2 @Tb/guanosine 5′-monophosphate (GMP) NCPs with core-shell nanostructures was designed for O 2 •− detection. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were produced by using SiO 2 nanoparticles (NPs) as template to control their nano-size. First, Tb 3+ was adsorbed on the surface of SiO 2 NPs by coordination with -COOH of SiO 2 NPs. Then the Tb/GMP NCPs shells were grown along the surface of SiO 2 NPs. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and Zeta potential. The resulted SiO 2 @Tb/GMP NCPs core-shell nanostructures displayed strong Tb/GMP emission which decreased linearly with the increased O 2 •− concentration in the range from 5.3 nM to 6.0 μM with a limit detection of 2.18 nM. And the SiO 2 @Tb/GMP NCPs core-shell nanostructures also exhibited good selectivity and high sensitivity in HEPES buffer at pH 7.4.
研究领域:
材料科学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Hao Liang,Feifei Lin,Zijie Zhang,Biwu Liu,Shuhui Jiang,Qipeng Yuan,Juewen Liu
Using nanomaterials to achieve functional enzyme mimics (nanozymes) is attractive for both applied and fundamental research. Laccases are multicopper oxidases highly important for biotechnology and environmental remediation. In this work, we report an exceptionally simple yet functional laccase mimic based on guanosine monophosphate (GMP) coordinated copper. It forms an amorphous metal–organic framework (MOF) material. The ratio of copper and GMP is 3:4 as determined by isothermal titration calorimetry. It has excellent laccase-like activity and converts a diverse range of phenol containing substrates such as hydroquinone, naphthol, catechol and epinephrine. Comparative work shows that the activity is originated from guanosine coordination instead of phosphate binding in GMP. Cu2+ is required and cannot be substituted by other metal ions. At the same mass concentration, the Cu/GMP nanozyme has a higher Vmax and similar Km compared to the protein laccase. To achieve the same catalytic efficiency, the cost of the Gu/GMP is ∼2400-fold lower than that of laccase. The Cu/GMP is much more stable at extreme pH, high salt, high temperature and for long-term storage. This is one of the first laccase-mimicking nanozymes, which will find important applications in analytical chemistry, environmental protection, and biotechnology.
研究领域:
生物技术
·
环境修复
·
分析化学
·
纳米材料
·
酶模拟
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）