CELLULAR & MOLECULAR BIOLOGY LETTERS
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2023
·Vol. 28
·期 1
·1-21
·
IF 8.3
Wu Jiangpeng,Sun Xiuping,Wu Chunyi,Hong Xiaoping,Xie Lulin,Shi Zixu,Zhao Liang,Du Qingfeng,Xiao Wei,Sun Jichao,Wang Jigang
Background Glyphosate (GLY), as the active ingredient of the most widely used herbicide worldwide, is commonly detected in the environment and living organisms, including humans. Its toxicity and carcinogenicity in mammals remain controversial. Several studies have demonstrated the hepatotoxicity of GLY; however, the underlying cellular and molecular mechanisms are still largely unknown. Methods Using single-cell RNA sequencing (scRNA-seq), immunofluorescent staining, and in vivo animal studies, we analyzed the liver tissues from untreated and GLY-treated mice. Results We generated the first scRNA-seq atlas of GLY-exposed mouse liver. GLY induced varied cell composition, shared or cell-type-specific transcriptional alterations, and dysregulated cell–cell communication and thus exerted hepatotoxicity effects. The oxidative stress and inflammatory response were commonly upregulated in several cell types. We also observed activation and upregulated phagocytosis in macrophages, as well as proliferation and extracellular matrix overproduction in hepatic stellate cells. Conclusions Our study provides a comprehensive single-cell transcriptional picture of the toxic effect of GLY in the liver, which offers novel insights into the molecular mechanisms of the GLY-associated hepatotoxicity.
研究领域:
毒理学
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分子生物学
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基因组学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Lan Feng,Nuohan Xu,Qian Qu,Zhenyan Zhang,Mingjing Ke,Tao Lu,Haifeng Qian
Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in industrial and agricultural production. Glyphosate is a broad-spectrum systemic herbicide, which mainly acts in the phloem of weeds that compete with crop growth and is widely used in agriculture. This study investigated the interactive effects of AgNPs and glyphosate on the physiological morphology, gene transcription, and rhizosphere microorganisms of wheat. Our results demonstrated that wheat growth, and the structure and diversity of rhizosphere microorganisms were slightly influenced by AgNPs and glyphosate single treatment at the test concentration. However, AgNPs and glyphosate (Gly) combined treatment (AgNPs + Gly) strongly inhibited wheat growth and influenced gene transcription. In total, 955, 601, and 1336 genes were determined to be differentially expressed in AgNPs, glyphosate, and combined treatment, respectively. According to KEGG analysis, the combined groups induced an antioxidant response by upregulating the transcription of phenylpropanoid biosynthesis-related genes. In addition, more energy was needed, and disrupted cell membrane was shown in the combined treatment, which displayed in the upregulation of sucrose, starch, and lipid synthesis. Moreover, the relative abundance of Bradyrhizobium , Devosia , Kribbella , Sphingopyxis (nitrogen-fixing bacteria), and Streptomyces (plant growth-promoting bacteria) in soil microbiota were decreased, implicated that nitrogen fixation and some beneficial substance secretions were inhibited by the combined treatment. This study emphasized that the synergetic effects of AgNPs and glyphosate exerted a negative impact on wheat growth.
研究领域:
植物学
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环境科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2021
·
IF 19.503
Yingping Huang,Zhangli Li,Kun Yao,Chuncheng Chen,Chaoyuan Deng,Yanfen Fang,Ruiping Li,Hailin Tian
The increasing presence of glyphosate has raised concerns about its potential impact on the environment and human health. The oxidative degradation of glyphosate during biotic and abiotic pathways usually leads to the formation of the toxic and persistent intermediates aminomethylphosphonic acid (AMPA). In this work, we report that the formation of AMPA during the photocatalytic degradation of glyphosate can be determined by its adsorption modes on the photocatalyst. The maximum accumulation of AMPA reached 31% on TiO 2 photocatalyst. However, the formation of AMPA was markedly suppressed in the presence of F – ions, while the production of inorganic species such as PO 4 3– and NO 3 – was largely enhanced. In situ attenuated total reflectance Fourier transforms infrared (ATR-FTIR) spectroscopy revealed that the glyphosate was adsorbed on surface of TiO 2 by its phosphate and carboxylate groups, while the adsorption site was changed to its amine group in the presence of F – ions. Such a change in the adsorption mode of glyphosate would facilitate the C–N bonds cleavage in glyphosate molecules, and inhibit the formation of AMPA, which makes the degradation of glyphosate go along a less toxic degradation pathway. The present study reveals a new relationship between the degradation pathway and the adsorption mode of glyphosate, which may also be helpful in other systems of abiotic degradation of glyphosate.
研究领域:
环境科学
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生物学
✓ 已验证
产品关联
DOI
高影响因子
Vietnam Journal of Chemistry
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2021
·Vol. 59
·期 6
·813-822
·
IF 0
Nguyen Trung Dung,Vu Dinh Thao,Nguyen Nhat Huy
In this study, the CuCoFe-layered double hydroxide was employed for the degradation of glyphosate in water via peroxymonosulfate (PMS) activation. Scanning electron microscopy, X-ray powder diffraction, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and Brunauer-Emmett-Teller analyses were used to characterize the material. The effects of the catalytic system, initial solution pH, and catalyst and PMS dose on the degradation efficiency of glyphosate were investigated. The results indicated that after 6 min the CuCoFe-LDH/PMS system can degrade 99.54 % of glyphosate (100 mg/L) using 100 mg/L of CuCoFe-LDH, 200 mg/L of PMS, and pH 11. In addition, radical quenching experiments prove that the reactive oxygen species (e.g., SO 4 ●- , HO ● , O 2 ●– , and 1 O 2 ) are formed and SO 4 ●- plays a decisive role in glyphosate degradation. Moreover, the CuCoFe-LDH catalyst is reusable and stable with a glyphosate removal efficiency of 90.34 % after five continuous degradation cycles.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
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2022
·Vol. 101
·期 6
·3262-3272
·
IF 2.5
Dongmei Jia,Jia Guo,Jing Li,Yongzheng Duan
The composite of CuO coated MIL-101(Fe) (CuO@MIL-101(Fe)) was successfully prepared using a one-pot hydrothermal technology. X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Brunauer–Emmett–Teller (BET), and TG technologies were used to characterize its structural, morphological, and physicochemical properties. CuO@MIL-101(Fe) exhibited a better pH adaptivity, a faster removal ratio of 95.8% for glyphosate within 10 min at pH 7, and a higher selectivity of adsorption capacity, even reaching 231.6 and 233.5 mg g −1 with coexisting Cl − (10 mmol L −1 ) and SO 4 2− (10 mmol L −1 ). The enhanced adsorption performances of CuO@MIL-101(Fe) may be caused by Cu forming a coordination interaction with glyphosate. A possible mechanism of glyphosate removal was discussed on the basis of the FTIR and X-ray photoelectron spectroscopy (XPS) analysis. CuO@MIL-101(Fe) composite would be more favourable to capture glyphosate from wastewater.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
VACUUM
·
2023
·
IF 4
Changjiang Qiu,Liqin Zhang,Fengjuan Miao,Bairui Tao,Hui Li,Zengcheng Qiu,Yu Zang
In addition to its low cost and stability, the sensor has numerous environmental sensing application possibilities. In this research, we use a ZnO/CuO structure that is structurally analogous to the PN junction, which speeds up electron flow and increases electrode sensitivity. We next modified the composite's surface with Gold Nanoparticles , so increasing the electrode's active area. We next examined the surface topography and microstructure of our manufactured electrodes using a variety of characterisation analytical methods. Finally, a series of electrochemical and photoelectrochemical studies were conducted to evaluate the Glyphosate sensing characteristics of the electrodes we had created.Testing revealed that the resulting electrodes had high electrical conductivity and, thus, enhanced sensitivity to Glyphosate. When compared to other herbicide sensors, the resulting electrode has a low detection limit and a decent detection range (6.9 μmol L −1 to 230 μmol L −1 ). Our manufactured sensor electrode has the potential to be a very sensitive composite electrode for use in modern herbicide detection sensors.
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Ivan Sazykin,Ekaterina Naumova,Tatiana Azhogina,Maria Klimova,Shorena Karchava,Ludmila Khmelevtsova,Elena Chernyshenko,Alla Litsevich,Margarita Khammami,Marina Sazykina
Glyphosate is the most widely used herbicide in the world. There is still no complete clarity about the degree of its genotoxicity and mutagenicity . In addition, its effect on bacterial biofilms, the main life form of soil microbial communities , has not been adequately studied. Toxicity and mutagenicity, as well as changes in the bacterial biofilm biomass, physiological activity, and the number of living cells in its composition in the presence of glyphosate were assessed using the Escherichia coli model. To assess damage to cellular components under the action of this pesticide, luminescent whole-cell bacterial lux-biosensors were used. Changes in the level of mutagenesis were studied by the method of rifampicin mutants. High integral toxicity of glyphosate, the average level of increased oxidative stress and protein damage were shown with the help of bacterial biosensors . All the studied concentrations of the pesticide completely or partially suppress the matrix and structure of the E. coli CDC F-50 biofilm formation, as well as the bacterial cells metabolic activity in the biofilm. At the concentrations of 6.7 and 0.67 g/L, glyphosate suppresses mutagenesis, probably due to general suppression of metabolism, and at the concentration of 0.0067 g/L, it enhances mutagenesis by six times compared with the spontaneous level. Suppression of bacterial biofilms formation, toxic effects on microorganisms , and mutagenesis enhancement by glyphosate can lead to negative consequences for natural microbiomes.
研究领域:
微生物学
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毒理学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2022
·Vol. 12
·期 10
·832
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IF 5.743
Yuliang Cheng,Guowen Li,Xiufang Huang,Zhijuan Qian,Chifang Peng
In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu2+-ion-modulated strategy, was developed. In the presence of Cu2+, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu2+concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0–50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection.Keywords:label free;fluorescence;turn on;DNA;Cu2+;silver nanoclusters
研究领域:
分析化学
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环境科学
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纳米技术
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
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IF 2.88
Meng-Lei Xu,Yu Gao,Yali Li,Xueliang Li,Huanjie Zhang,Xiao Xia Han,Bing Zhao,Liang Su
Glyphosate is one of the most commonly-used and non-selective herbicides in agriculture, which may directly pollute the environment and threaten human health. A simple and effective approach to assessment of its damage to the natural environment is thus quite necessary. However, traditional chromatography-based detection methods usually suffer from complex pretreatment procedures. Herein, we propose a simple and sensitive method for the determination of glyphosate by combining ninhydrin reaction and surface-enhanced Raman scattering (SERS) spectroscopy. The product (purple color dye, PD) of the ninhydrin reaction is found to SERS-active and directly correlate with the glyphosate concentration. The limit of detection of the proposed method for glyphosate is as low as 1.43 × 10 − 8 mol·L − 1 with a relatively wider linear concentration range (1.0 × 10 − 7 -1.0 × 10 − 4 mol·L − 1 ), which demonstrates its great potential in rapid, highly sensitive concentration determination of glyphosate in practical applications for safety assessment of food and environment.
研究领域:
农业科学
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分析化学
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环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Zilu Li,Xingyu Zhang,Yue Wang,Zirui Zheng,Chenhui Zhang,Tianyue Wu,Yanling Wu,Yuxia Gao,Fengpei Du
Glyphosate, one of the most widely used herbicides, plays an important role in controlling weeds and ensuring crop production. While using glyphosate, adjuvants are commonly added to improve its deposition on weeds and control efficacy. However, changes in weed leaf surface characteristics may reduce glyphosate penetration and contribute to evolved glyphosate resistance. Therefore, it is significant to introduce an improved method for regularizing leaf surface characterization and guide adjuvant selection to improve glyphosate efficacy. In this work, surface characteristics of typical weed leaves have been systematically investigated by 3D surface analysis and scanning electron microscopy, finally quantified by apparent surface free energy (ASFE) due to its comprehensive and quantitative evaluation of leaf surfaces. Moreover, the relationship between the weed leaf surface characteristics and the retention of glyphosate on weeds was established, further related to the control efficacy against weeds. To maximize the utilization rate of glyphosate, the types and concentrations of adjuvants should be regulated according to the ASFE of weeds. Our findings not only regularize the surface properties of weed leaves but also reveal their influencing mechanism on the deposition and biological activity of glyphosate, which provide effective guidance for the use of glyphosate.
研究领域:
植物学
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农学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 41
·期 3
·732-739
·
IF 2.557
Dan Feng,Yan Xia
A Cr-based metal–organic framework, namely, MIL-101(Cr), was modified with amino (NH 2 –) and urea (UR 2 –) groups, and the materials were evaluated as adsorbents for glyphosate, and a comparison with commercial activated carbon was also discussed. The effects of the adsorption factors, such as adsorbent concentration, adsorption time, pH and ionic strength were mainly investigated. The results showed that a pseudo-second-order rate equation described the adsorption kinetics mechanisms well, while the Langmuir model and the Freundlich model fitted different adsorption isotherms, respectively. Among the adsorbents we studied, NH 2 -MIL-101(Cr) showed the maximum adsorbing capacity, which is 64.25 mg/g when pH = 3.0, while UR 2 -MIL-101(Cr) did not reach the best adsorption performance due to the steric hindrance. The work opens up a new way for the modification of metal–organic frameworks for adsorption process.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Rui Jiang,Yue-Hong Pang,Qiu-Yu Yang,Chao-Qun Wan,Xiao-Fang Shen
Glyphosate (GLY), an exceedingly effective herbicide, has raised worries about the risks posing to the ecosystem and human health due to excessive exposure. An electrochemical sensor based on copper porphyrin metal organic framework and gold nanoparticles modified carbon paper (Cu-TCPP/AuNPs/CP) was developed for sensitive determination of GLY by differential pulse voltammetry (DPV). The Cu-TCPP has large surface area and excellent catalytic activity , increasing the copper sites to combine with GLY for selective determination and improving the sensitivity of the electrochemical sensor. Under optimized conditions, the constructed sensor has a linear range from 0.2 to 120 μ mol L −1 with a detection limit of 0.03 μ mol L −1 (S/N = 3). The sensor was applied to water, soybean, wheat and carrot with the recovery ranging from 97.5% to 110.7%. After two weeks storage at 4 °C, the peak current of the sensor remains 86.6% of the original value with negligible interference. This sensor has a great potential application in water, soil and other environmental samples.
研究领域:
环境科学
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分析化学
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材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）