TALANTA
·
2018
·
IF 4.244
Zi-Han Chen,Xin-Yue Han,Ling-Xue Deng,Zi-Yang Lin,Fang-Ya Mu,Shengqiang Zhang,Guoyue Shi,Min Zhang
Tb 3+ -doped carbon dots (Tb 3+ @CDs) were prepared in a facile hydrothermal method by using ammonium citrate as carbon source and Tb 3+ as dopant. A 15-bp GT-rich single-strand DNA (ssDNA) was introduced to sensitize Tb 3+ via the antenna effect for generating two fluorescence signals (CDs and Tb 3+ ), forming a conjugate of Tb 3+ @CDs/ssDNA. The ratiometric fluorescence of Tb 3+ @CDs/ssDNA could be reversibly regulated by Ag + and Cys, in which the fluorescence peak at 546 nm of Tb 3+ could be switched to “On” or “Off” as the signal indicator while the fluorescence peak at 444 nm of CDs remained constant as the build-in reference. The proposed Ag + /Cys-mediated reversible fluorescence changes in Tb 3+ @CDs/ssDNA was also proven for the design of a self-calibrating ratiometric fluorescence logic system. By integrated with the specific reaction between H 2 O 2 and Cys, Tb 3+ @CDs/ssDNA was applied for ratiometric fluorescence detection of H 2 O 2 . More importantly, the sensing strategy could be further successfully extended to the monitoring of H 2 O 2 -produced oxidase-related reactions, such as GOx-biocatalyzed oxidation of glucose (the limit of detection: 0.06 μM) and was well applied in rat serum compared to commercial kits. This work unveiled a novel ratiometric fluorescent design, which is cost-effective, simple to prepare and easy-to-use without chemical modification or fluorescence labeling .
研究领域:
生物化学
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 14
·6700-6708
·
IF 3.269
Huimin Zhao,Yaxi Li,Bing Tan,Yaobin Zhang,Xiaochi Chen,Xie Quan
To improve the peroxidase-like activity of molybdenum dichalcogenides (MoS2), surface modification, such as combing with monometallic or bimetallic nanoparticles, is of great and widespread interest. However, only a few studies have been reported on the modification of MoS2 with non-metallic materials. Herein, we report a study on the peroxidase-like activity of novel PEGylated MoS2 (PEG-MoS2) nanosheets prepared using a simple liquid-phase exfoliation and pegylation process. Compared to the undecorated MoS2 nanosheets, PEG-MoS2 nanosheets have improved water dispersiblity and stability, which are beneficial for improving the peroxidase-like activity, as well as the catalysis velocity and affinity for TMB or H2O2. Based on PEGylated MoS2, we successfully established a new platform for the colorimetric detection of H2O2 with a limit of detection (LOD) of 1.18 μM, which is comparable to that of the majority of the reported enzyme mimics of inorganic graphene analogues. More importantly, the as-prepared PEG-MoS2 nanosheets are environmentally friendly, water dispersible, low cytotoxic, and easily prepared, which indicate that PEG-MoS2 is a type of promising enzyme mimics and extremely likely to be applied in cell-based assay systems without any adverse reactions.
研究领域:
生物化学
·
纳米医学
·
分析化学
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Tan Jingying,Geng Weifu,Li Junde,Wang Zhen,Zhu Shaohao,Wang Xiuzhong
The mimic enzyme has become a research hotspot in recent years because of its advantages of high stability, convenient preparation, and low price. In this article, Co3O4 nanosheets synthesized by a simple hydrothermal method possess the characteristics of a peroxidase-like activity. The results demonstrated that 3,3′,5,5′-Tetramethylbenzidine (TMB) could be oxidized by H2O2 to produce a typical blue product (oxTMB) which has a strong absorption at 650 nm wavelength with the help of the Co3O4 nanosheets. Thus, a simple and sensitive colorimetric detection method for H2O2 was established with a good linear relationship (2–200 μM) and a low limit of detection (0.4 μM). Meanwhile, the colorimetric product can effectively quench the fluorescence emitted by Ru(bpy)32+. Therefore, a colorimetric and fluorescence dual detection mode photochemical sensor for H2O2 detection is constructed based on the principle of the inner filter effect (IFE) between the colorimetric product (oxTMB) and Ru(bpy)32+. It can effectively avoid the false positive problem of a single detection mode. In the presence of glucose oxidase, glucose can be catalyzed to produce gluconic acid and H2O2; therefore, the sensor can also be used for the determination of glucose with a good linear relationship (0.02–2 μM) and a low limit of detection (5 nM). Experimental results showed that the sensor has a high sensitivity and strong anti-interference ability which can be used for the detection of actual samples.
研究领域:
生物传感器
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Chunqiu Xia,Xiaomin Zhang,Xiaotao Liu,Dehuai Wu,Liangqia Guo
2, 2′-azinobis-3-ethylbenzthiazoline-6-sulfonate (ABTS), 3, 3′, 5, 5′-tetramethylbenzidine (TMB), o-phenylenediamine (OPD) are typical chromogenic substrates to evaluate the activity of peroxidase-like nanozymes. However, these substrates are toxic, unstable, and the coloration reactions are slow. Herein, the classical starch-sodium iodide reagent was utilized as the chromogenic substrate to evaluate peroxidase-like activity of nanozymes. Starch-sodium iodine is less toxic, more stable and faster color reaction than ABTS, TMB and OPD. Under the catalysis of peroxidase-like nanozymes such as Mo 5 N 6 nanosheets , graphene oxide nanosheets, Fe 3 O 4 nanoparticles , CeO 2 nanoparticles, iodide ion (I – ) is oxidized by H 2 O 2 to produce blue color complex (starch-I 2 ). Taking Mo 5 N 6 nanosheets as the peroxidase-like nanozyme, the color reaction completed within 5 min at room temperature (25 ºC), and the chromogenic substrate remained stable within 30 days. The maximum reaction rate (V max ) using starch-sodium iodine as the chromogenic substrate was more than 100-fold higher than that of TMB. Based on this, a sensitive colorimetric sensor and facile portable agarose gel test kits were proposed and applied for the detection of H 2 O 2 residue in liquid milk samples with fast response.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Bin Li,Heng Liu,Xianrong Xing,Caixia Zeng,Min Zhang,Hailong Li,Na Lu
The development of sensitive and accurate glucose biosensors is significant in diabetic patient monitoring. Here, we synthesize carbon-decorated Fe3O4 nanotubes (Fe3O4@C NTs) using a rational designed template method. Through catalytic oxidation of a typical colorimetric reaction, the prepared Fe3O4@C NTs showed significantly better peroxidase-like and oxidase-like performances than other similar Fe3O4-based nanomaterials. Moreover, the optimal pH, temperature, and concentration for Fe3O4@C NTs were 4.0, 60 °C, and 40 μg/mL, respectively. We additionally investigated the steady-state kinetics of Fe3O4@C NTs, and their catalyzed reactions were found to follow the typical Michaelis–Menten model. Based on the peroxidase-like activity of Fe3O4@C NTs, they were employed for label-free, rapid, selective, and highly sensitive colorimetric detection of H2O2 and glucose, demonstrating a potential application for glucose determination.
研究领域:
生物传感技术
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材料科学
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医学诊断
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 23
·12518
·
IF 4.9
Mingyu Jia,Jueyu Wang,Yuxuan Liu,Daizong Cui,Min Zhao
In this study, Fe3(PO4)2·8H2O magnetic nanoparticles (MNPs) were successfully extracted from the strainBurkholderia cepaciaCG-1. We subsequently characterized their composition, structure, and morphology, revealing that these nanoparticles consisted of Fe3(PO4)2·8H2O with an average diameter of 66.87 ± 0.56 nm. Our measurements indicated magnetic parameters of 151 Oe for coercivity, 2 emu/g for saturation remanence, and 16 emu/g for saturation magnetization. Our findings confirmed that these magnetic nanoparticles exhibited intrinsic peroxidase-like activity, catalyzing the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2. Compared to horseradish peroxidase (HRP), the peroxidase mimic demonstrated greater stability under various physicochemical conditions. To investigate the mechanisms underlying the peroxidase-like catalysis of Fe3(PO4)2·8H2O, we employed fluorescence spectroscopy and electron spin resonance (ESR) analysis. The results revealed that the peroxidase-like activity of Fe3(PO4)2·8H2O stemmed from the generation of hydroxyl radicals (·OH). Furthermore, we established a platform for the colorimetric detection of H2O2and glucose. Our method was capable of detecting H2O2concentrations as low as 1.0 × 10−3mmol/L. Impressively, this sensitive method was successfully applied to determine glucose levels in human serum.
研究领域:
生物化学
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纳米医学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2022
·
IF 4.177
Chengyu Zhang,Zhaodong Nan
Nanoenzymes have been regarded as strong substitutes to natural enzymes in the last decades. However, high concentrations of substrates are always needed because of the low activity of nanozymes, which causes an increase of the cost and an increase in environmental pollution in practical applications. Thus, improved nanozymic activity is still urgent and vital to reduce the need for high concentrations of substrates. A series of binary metal sulfides (FexNi1–xS2), which exhibited intrinsic nanozymic activity, was first synthesized by a hydrothermal method. Among these samples, the nanosheet-shaped Fe0.8Ni0.2S2 showed the highest activity. Compared with those of FeS2, the catalytic constant (Kcat) and catalytic efficiency (Kcat/Km) of Fe0.8Ni0.2S2 increased about 1.9 and 3.6 times, respectively. The Michaelis–Menten constant (Km) of Fe0.8Ni0.2S2 is about 4.7-fold smaller than that of FeS2, and 193-fold smaller than that of natural enzyme horseradish peroxidase (HRP), indicating a stronger affinity to H2O2. The investigation on the mechanism indicated that Fe2+ and Fe3+ in Fe0.8Ni0.2S2 are the active sites. The synergistic effect between Ni2+ and Fe3+ accelerated the conversion from Fe3+ to Fe2+, which improved the nanozymic activity. In addition, Fe0.8Ni0.2S2 can be used as a nanozyme to detect H2O2 and ascorbic acid (AA) within 1 min at room temperature (25 °C). This work extends the development of binary metal sulfides as artificial enzymes.
研究领域:
生物化学
·
材料科学
·
催化反应
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DOI
高影响因子
Journal of Analysis and Testing
·
2019
·Vol. 3
·期 3
·238-245
·
IF 0
Sun Shihao,Fan Yaofang,Du Jiaoyang,Song Zhilian,Zhao Huimin
Over the past few decades, the enzyme-mimicking activity of metal–organic frameworks (MOFs) accompanied with structural characteristics has aroused much attention. However, pure MOFs have low affinity with DNA. Here, iron-based MOFs with acidized carbon nanotubes (CNTs) via a simple hydrothermal process have been synthesized, named as MIL-88(NH 2 )-Fe@CNTs. CNTs can enhance the affinity between MOF and DNA, achieving flexible regulation of their catalytic activity benefiting from the strong π − π stacking between CNTs and DNA. Meanwhile, in comparison with conventional iron-based MOFs, the addition of CNTs, which contributes to the acceleration of electron transfer, endowing as-prepared nanocomposites remarkably enhanced peroxidase-like activity to achieve an ultrasensitive detection of H 2 O 2 with the LOD of 17.64 μg/L. Notably, the as-prepared nanocomposites with adsorbed DNA displayed excellent affinity towards both TMB (3, 3′, 5, 5′-tetramethylbenzidine) substrates and H 2 O 2 as well as high catalytic velocity. On the basis of their switchable peroxidase-like activity regulated by different length or sequence of ssDNA, it is believed that our-prepared MOF-based nanomaterials would be promising for fabricating versatile and sensitive label-free colorimetric assays for diverse targets.
研究领域:
生物化学
·
纳米材料科学
·
分析化学
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产品关联
DOI
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2016
·Vol. 34
·期 3
·1600233
·
IF 4.367
Yanzhou Jiang,Yan Gu,Guangdi Nie,Maoqiang Chi,Zezhou Yang,Ce Wang,Yen Wei,Xiaofeng Lu
In this paper, a novel strategy for the fabrication of reduced graphene oxide (rGO)/Cu 8 S 5 /polypyrrole (PPy) composite nanosheets with Cu 8 S 5 nanoparticles and PPy layer anchored on the surface of rGO as peroxidase-like nanocatalyst is reported. During the synthesis, graphene oxide (GO)/CuO composite nanosheets are prepared first and used as templates, then the sulfuration of CuO and polymerization of pyrrole are accompanied with the reduction of GO, resulting in ternary rGO/Cu 8 S 5 /PPy composite nanosheets. The synthesized Cu 8 S 5 nanoparticles with a diameter in the range from tens to hundreds of nanometers are dispersed within PPy decorated rGO nanosheets. The resultant ternary rGO/Cu 8 S 5 /PPy composite nanosheets exhibit a higher peroxidase-like catalytic activity toward the oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H 2 O 2 than GO/CuO and rGO/CuS composite nanosheets, revealing a synergistic effect on their activity. The as-prepared rGO/Cu 8 S 5 /PPy platform provides a simple colorimetric approach for the detection of H 2 O 2 and phenol with a high sensitivity. This work offers a new way for the fabrication of rGO-based nanocomposite with superior enzyme-like activity, which displays great potential applications in biocatalysis and environmental monitoring.
研究领域:
材料科学
·
生物催化
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 9
·2097-2105
·
IF 1.915
Junrong Li,Liang Lv,Guannan Zhang,Xiaodong Zhou,Aiguo Shen,Jiming Hu
We herein present a simple and surfactant-free synthetic strategy for the preparation of core–shell Fructus Broussonetia-like Au@Ag@Pt nanoparticles (FBNPs) via a combination of galvanic replacement and reagent reduction, in which a chitosan (CS)-mediated epitaxial growth of Pt multi-branches occurs on cresyl violet labeled Au core–Ag shell nanoparticles (NPs) at room temperature. The as-prepared novel nanostructures exhibit an enzyme mimetic activity due to the formation of a Fructus Broussonetia-shaped Pt shell, the absence of surfactants and the positively charged CS molecules intertwined within the outermost Pt NPs. Relying on the high sensitivity of resonance-enhanced Raman scattering and the highly efficient intrinsic peroxidase-like activity of FBNPs, H2O2 was detected with a wide detection window from 10 pM to 100 μM when utilizing 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrate. Based on the H2O2–TMB catalytic oxidation system a simple, sensitive, selective and universal platform has been extended for the detection of all peroxidase-related analytes. For example, glucose at a concentration range of 1 nM to 200 μM was detected in the presence of glucose oxidase with a limit of detection of 1 nM.
研究领域:
纳米材料
·
生物分析化学
·
医学诊断
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产品关联
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 8
·1111-1124
·
IF 2.378
Xiaoheng Qi,Huiming Tian,Xueming Dang,Yaofang Fan,Yaobin Zhang,Huimin Zhao
Metal–organic frameworks (MOFs) with a metal coordination structure mimicking that of enzymes have been widely applied in the sensing field for bioassays, and most of the reports focus on single-metal MOFs. However, bimetallic MOFs have fascinating catalytic capabilities due to their bimetallic coordination. Herein, a two-dimensional sheet-like bimetallic Co/Mn-based MOF (Co/Mn-MOF) with peroxidase-like activity was synthesized through a one-step hydrothermal method to accelerate the decomposition of H2O2 with the generation of ˙OH, which further oxidized the classic chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) into oxTMB. The Michaelis–Menten constants (Km) and maximum initial velocities (Vmax) of the catalyst were found to be 0.27 mM and 1.64 × 10−7 M s−1 for TMB and 0.24 mM and 5.80 × 10−7 M s−1 for H2O2, respectively. Accordingly, we successfully utilized Co/Mn-MOF-1-1-150 to establish a colorimetric detection platform for the detection of H2O2. A linear absorbance response ranging from 1 to 100 μM with the limit of detection of 0.85 μM (S/N = 3) was obtained under the optimized conditions. The LOD of our catalyst is comparable to that of other MOF-based enzyme mimics for colorimetric detection; this can be ascribed to the synergistic catalytic effect between its two metals, its two-dimensional structure with a small diffusion barrier for the substrate molecules, and large exposed surface area. More importantly, using the proposed sensing method, the determination of H2O2 released by living cells and in spiked water samples was achieved. This revealed that the as-prepared bimetallic Co/Mn-MOF could be a promising candidate as a catalytic material due to its peroxidase activity.
研究领域:
材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·E210
·
IF 4.034
Qingtong Zhang,Mingfu Li,Chenyan Guo,Zhuan Jia,Guangcong Wan,Shuangfei Wang,Douyong Min
Lignin is the second largest naturally renewable resource and is primarily a by-product of the pulp and paper industry; however, its inefficient use presents a challenge. In this work, Fe₃O₄ nanoparticles loaded on lignin nanoparticles (Fe₃O₄@LNPs) were prepared by the self-assembly method and it possessed an enhanced peroxidase-like activity. Fe₃O₄@LNPs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate a blue color, was observable by the naked eye. Under the optimal conditions, Fe₃O₄@LNPs showed the ability of sensitive colorimetric detection of H₂O₂within a range of 5⁻100 μM and the limit of detection was 2 μM. The high catalytic activity of Fe₃O₄@LNPs allows its prospective use in a wide variety of applications, including clinical diagnosis, food safety, and environmental monitoring.
研究领域:
生物化学
·
纳米材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）