ANALYTICAL LETTERS
·
2023
·
IF 2
Yi Wu,Guobao Zhou,Zeyu Ma,Dan Zhang,Jianrong Chen,Lei Li
Deep understanding of the effect of DNA sequence and structure on the fluorescent properties of DNA-templated silver nanoclusters (DNA–Ag NCs) affords alternative strategies for developing and exploiting DNA biosensors. DNA secondary structures (e.g., DNA loop) are essential for generating highly emissive DNA–Ag NCs. In this study, a cytosine-rich DNA loop was used as a facile template for the formation of silver nanoclusters. The fluorescence of silver nanocluster encapsulated within a cytosine-rich DNA loop was turned on by switching the size of the loop from large to small. Target nucleic acid served as the trigger for the hybridization chain reaction of hairpin probe 1 (H1) containing an inactivated cytosine-rich DNA loop with a large size at the ends and hairpin probe 2 (H2). Long-nicked double helices were obtained coupled with the opening of the large-size cytosine-rich DNA loop in H1 and switching to an activated cytosine-rich DNA loop with a small size. As a result, an increase in the fluorescent signal was observed. A sensitive detection limit of 10 pM and a linear detection range from 10 pM to 400 nM were obtained for nucleic acid determination. This facile assay provides a new approach capable of switching the fluorescence emission of DNA–Ag NCs for nucleic acid biosensing.
研究领域:
生物化学
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分子生物学
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分析化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Yue Hou,Zensheng Liu,Shuyue Ren,Shuang Li,Kang Qin,Yuan Peng,Yu Wang,Dianpeng Han,Shiping Yang,Huanying Zhou,Zhixian Gao
Aflatoxins are highly carcinogenic and mutagenic. Among them, aflatoxin M1 (AFM1) is a hydroxylated metabolite that seriously poses threats to human health and safety, so there is a need to construct an efficient and sensitive detection system for AFM1. In this study, an analytical method was established for ultra-sensitive determination of AFM1 in food based on dendritic hybridization chain reaction (HCR) dual-amplification. The prepared carboxylated silica was modified with AFM1 antibody, dendritic (S) chain and initiator (H0) chain as fluorescent probes, the whole AFM1 antigen was coated on the microplate, and the antibody on the fluorescent probes competed with the AFM1 standard for binding to the whole antigen. Then the fluorescence response value of the supernatant was measured, which displayed a good linear relationship with the standard concentration. The detection range was 10 pg/mL-10 μg/mL, and the limit of detection was 5.41 fg/mL, which was in line with the limit value of China's national standard. In addition, the spiked recovery rate was between 84.77% and 115.70%. The method developed in this study displays an increase of three orders of magnitude in comparison with indirect competitive enzyme-linked immunosorbent assay (ic-ELISA), in the detection of AFM1, showing ultra-high sensitivity, which satisfies the needs of AFM1 analysis and detection in food. This biosensing method also has reference value for the detection of other toxins in food.
研究领域:
食品科学
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分析化学
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毒理学
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生物技术
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DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Zhen Song,Qiang-Yan Zhang,Jia-Jing Li,Jing-Lian Su,Yuan-Hua Liu,Gong-Jun Yang,Huai-Song Wang
Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was initiated by let-7a via two hairpin primers (H1 and H2). After the HCR, the remaining hairpin H1 was further detected by lateral flow assay (LFA) and electrochemical impedance spectroscopy. For LFA, biotin-modified H1(bio-H1) and free H2 were used for HCR. With the decrease of let-7a concentration, the color of T line gradually increased. As for electrochemical methods, the H1′-AuNP-modified electrode was used for detection of bio-H1 based on the difference of impedance (ΔRct) detected without and with different concentrations of let-7a participating in the HCR. This method could detect let-7a in the range of 10.0 fM and 1.0 nM with detection limits of 4.2 fM.
研究领域:
癌症诊断
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Linyuan Zhai,Xinyi Feng,Huali Jin,Tian Bai,Min Wei,Yong Liu,Zhiguang Suo
Cadmium ions (Cd 2+ ) as a class of widespread pollutants, can cause Irreversible damage to population health and habitat quality with small amounts of Cd 2+ . an electrochemical sensor for Cd 2+ detection was developed based on AuPt NPs@ZnNi-MOF bimetallic nanocomposites combined with hyperbranched hybridization chain reaction (HCR) signal amplification strategies. The sensor employed AuPt NPs@ZnNi-MOF as the electrode modification material, which promoted the electron transport on the electrode surface and significantly increased the current signal by 49.16 %, as well as provided a large number of active sites for the sensor. 3D DNA walker was initiated in the presence of the target, generating many DNA single strands that triggered catalytic hairpin self-assembly (CHA) at the electrode. The production of intermediate strand output is induced, which leads to the construction of hyperbranched HCR. Hyperbranched HCR amplification demonstrated high efficiency, producing numerous long double-stranded chains and creating abundant embedding sites for electroactive molecules. These signal amplification strategies led to a remarkable 2.52-fold enhancement in the current signal. Under the optimal experimental conditions, the detection range of the sensor was 0.001–500 nmol/L, and the limit of detection (LOD) was 0.221 pmol/L. Furthermore, the sensor exhibits excellent specificity, reproducibility, and stability, and has been successfully applied for the detection of Cd 2+ in tap water and tea, as well as achieving satisfactory results in the analysis of quality control samples such as rice flour and pork liver powder.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Panxue Wang,Ying Liu,Xiang Li,Cen Li,Guoliang Li
Rapid and reliable determination of pathogenic bacteria is crucial for food safety and public health. Surface-enhanced Raman spectroscopy (SERS) based aptasensors have attracted increasing attention in the determination of pathogenic bacteria due to their high specificity and sensitivity. However, the sensitivity and reliability of the SERS aptasensors for the determination of pathogens were hindered by the uneven distribution of SERS tags. To tackle this limitation, a SERS aptasensor based on a hybridization chain reaction (HCR) mediated signal conversion strategy was fabricated for the determination of E. coli O157:H7. In the presence of E. coli O157:H7, the blocked aptamer specifically binds with it and releases the blocker DNA. Then the blocker DNA hybridizes with the capture DNA on the SERS probe and initiates the HCR reaction, which further leads to the formation of double stranded DNA and the reduction of the SERS signal intensity of 4-mercaptobenzonitrile (4-MBN). Under the optimal conditions, the SERS signal intensity at 1073.9 cm −1 and the logarithmic value of the concentration of E. coli O157:H7 in the range of 8 × 10 2 –8 × 10 7 CFU·mL −1 exhibited a good linear relationship, with a coefficient of determination of 0.92. The limit of detection for E. coli O157:H7 was 409 CFU·mL −1 . Besides, this aptasensor exhibited satisfactory specificity for E. coli O157:H7. In addition, it showed excellent stability in real sample analysis with relative standard deviations (RSDs) below 3.76 % (n = 3). The established SERS aptasensor employed a HCR mediated signal conversion strategy and overcame the drawback of unevenly distribution of SERS tags in classical SERS aptasensors for the determination of pathogenic bacteria. This study offered a new reference for the rapid and reliable detection of pathogens with a portable Raman spectrometer.
研究领域:
食品安全
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生物分析化学
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微生物学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yanli Zhu,Jikai Wang,Haitao Xie,Hailing Liu,Shuangquan Liu,Dongxiu He,Pengbing Mi,Suisui He,Jun Wang,Yiyang Sun
Sub-10 nm monodisperse alkaline-earth sulfide nanodots (ASNDs) with bright near-infrared (NIR)-excitation fluorescence and adjustable emission wavelength were prepared by a thermal decomposition method for the first time. The ASNDs exhibited high NIR-to-vis conversion efficiency and served as multicolor fluorescent labels in the proposed miR-224 assay. Targeted detection of the miR-224 level and single-nucleotide variation in miR-224 was carried out on a smartphone-based platform using a hybridization chain reaction (HCR) amplification strategy. In the presence of miR-224, the ASND-labeled HCR probes self-assembled on the surface of the diagnosis kits, generating strong fluorescent signals linearly proportional to miR-224 contents in the range of 10–2000 fM. Significantly, mutations in miR-224 led to the variation in the fluorescence intensity ratio in RGB channels. Simultaneously, evident changes of fluorescent brightness and color were easily visualized by the naked eye, which enabled on-site discrimination of miR-224 with different mutant loci. This work provides a novel preparation approach for ultrasmall NIR excitation sulfide nanodots and reveals the potential of the as-synthesized ASNDs in point-of-care (POC) nucleic acid testing. Further, it may provide a handheld platform for miRNA single-nucleotide polymorphism analysis.
研究领域:
生物化学
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纳米技术
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Yao Li,Li Yulin,Cheng Kewen,Pan Daodong,Xu Jianguo,Chen Wei
The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm −1 , increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM.
Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.
研究领域:
分析化学
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纳米技术
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生物传感器
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分子诊断
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
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IF 6.306
Miaolin Duan,Xiaoyue Xiao,Yanmei Huang,Guoqiang Li,Shan Shan,Xi Lv,Houde Zhou,Silu Peng,Chengwei Liu,Daofeng Liu,Weihua Lai
Escherichia coli O157:H7 makes a major threat to human health. Aiming to detect Escherichia coli O157:H7 sensitively, hybridization chain reaction signal amplified immunoassay (immuno-HCR) based on contact quenching (CQ) and fluorescence resonance energy transfer (FRET) was developed. The background of the new designed HCR hairpins (CQ-FRET hairpins) was reduced by contact-quenching fluorescein (FAM) and breaking FRET from donor (FAM) to acceptor (Cy5). The F/F 0 ratio of CQ-FRET hairpins (37.02) was obviously higher than that of two other common HCR fluorescent hairpins (CQ hairpins, 21.45; FRET hairpins, 4.61). The limit of detection of the assay was 3.5 × 10 1 CFU/mL and obviously lower than that of CQ hairpins based immuno-HCR (3.28 × 10 3 CFU/mL) and FRET hairpins based immuno-HCR (6.49 × 10 4 CFU/mL). The proposed low fluorescent background immuno-HCR with high sensitivity which was verified in contaminated milk samples could be potentially used in the detection of various pathogens.
研究领域:
微生物学
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生物技术
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Haoran Zheng,Jiayu Guo,Han Wang,Yurou Wang,Wenxiang Zhou,Ke Liu,Qiang Ma,Yanfei Qi
Bacteria are prevalent environmental pollutants. Live bacteria can proliferate and spread under appropriate conditions, presenting higher risks compared to non-viable counterparts. However, detecting live bacteria remains a challenge. Adenosine triphosphate (ATP), an energy currency in organisms, offers a reliable biomarker for the live bacteria sensing. Herein, we developed a zeolitic imidazolate framework-90 (ZIF-90) enhanced ATP sensing platform to detect live bacteria. The nanosystem which based on ZIF-90, encapsulating the DNA decorated magnetic beads (MB@S1) through self-assembly. When the S. aureus aptamers on ZIF-90 bonded to bacteria, the skeleton structure of ZIF-90 was disrupted by ATP leakage from live bacteria, leading to the release of MB@S1. Then, the MB@S1 initiated the hybridization chain reaction (HCR) and they were transduced by CRISPR-Cas12a to amplify the signal twice. The proposed ZIF-90 blocking sensing strategy (ZIF-90 strategy) exhibited remarkable sensitivity with the limit of detection (LOD) down to 0.223 pM for ATP detection, about 500 folds lower than the traditional ATP aptamer blocking sensing strategy (aptamer strategy). Furthermore, we used a smartphone for on-site analysis, realizing the quantification of live S. aureus with the LOD of 2.0 CFU/mL. Therefore, the approach possessed great application potential for public health, environment monitoring, bioanalysis and food safety.
研究领域:
生物化学
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分子生物学
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纳米医学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ye Tang,Dongyan Li,Ziyin Lu,Yaxi Wang,Wenjie Liu,Guoxing Jing,Wen Liu,Jianna Yu,Wenshan Li
The severe toxicity of Pb 2+ to human health, even at trace levels, the development of highly sensitive and dependable analytical methods remains of great importance. Here, we developed a label-free and enzyme-free colorimetric method for Pb 2+ assay by integrating a GR-5 DNAzyme recognition event with a catalytic hairpin assembly (CHA)-hybridization chain reaction (HCR) cascade amplification. Upon exposure to Pb 2+ , the GR-5 DNAzyme is specifically activated, catalyzing the site-specific cleavage of hairpin H1 to release the initiator strand T. The liberated T strand subsequently drives the CHA and HCR cascade reactions, generating long DNA superstructures enriched with multiple G-rich sequences. In the presence of hemin, these G-rich sequences fold into catalytically functional G-quadruplex/hemin DNAzymes, which enable a visible colorimetric signal generation. Under optimized conditions, the proposed colorimetric method delivered a linear response over the range of 50 pM to 50 nM and achieved a limit of detection of 16.71 pM. The practical applicability of the method was further demonstrated through Pb 2+ analysis in drinking water and fish samples, where satisfactory accuracy and reliability were obtained. In addition, inductively coupled plasma mass spectrometry was employed to validate the real-sample results, and the two methods showed good consistency. Owing to its simplicity, high sensitivity, and enzyme-free design, this colorimetric method shows strong potential for practical Pb 2+ monitoring in food safety and environmental analysis.
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-10
·
IF 5.3
Ren Dongxia,Wang Lin,Wei Hua,Mu Shijie,Yang Longfei
Bacterial infection is one of the leading causes of human death globally and is considered to be an urgent public health challenge. It is important to accurately and rapidly detect and kill pathogenic microorganisms. Therefore, a multifunctional platform for sensitive detection and efficient elimination of pathogenic bacteria should be developed to reduce the threat of bacteria. In this study, an integrated strategy based on Fe 3 O 4 -quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe 3 O 4 -QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) was proposed for the detection and inactivation of Staphylococcus aureus ( S. aureus ). The aptamer-complementary chain (aptamer-CP) conjugated to the surface of Fe 3 O 4 -QCS-PEI-Cu induces CP release in the presence of S. aureus , further initiating the ladder-branch HCR reaction and achieving signal amplification. Under optimal conditions, the developed sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and exhibited high selectivity against several other pathogenic bacteria. Moreover, the Fe 3 O 4 -QCS-PEI-Cu-aptasensor showed potent antibacterial properties under near infrared (NIR) irradiation, achieving an antimicrobial efficiency of 99.946%. This strategy synergistically combined the high-sensitivity molecular detection with the antibacterial activity of novel nanomaterials activated by near infrared light, providing a new insight into the prevention of bacterial infections. Graphical
研究领域:
生物医学工程
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微生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yong Wang,Dandan Wang,Guohua Qi,Ping Hu,Erkang Wang,Yongdong Jin
As one of the most widely distributed microRNAs, microRNA-21 (miRNA-21) significantly regulates target genes’ expression levels and participates in many cellular and intercellular activities, and its abnormal expression is always related to some diseases, especially cancer. Hence, detecting miRNA-21, as a biomarker, at the single-cell level helps us to reveal cell heterogeneity and expression level variation during the state change of cells. In this study, we constructed a gold nanoparticles nanomembrane (AuNPs-NM)-modified plasmonic glass nanopipette (P-nanopipette) surface-enhanced Raman scattering (SERS) sensing platform to sensitively detect content variation of the intracellular miRNA-21 during the electrostimulus (ES)-induced apoptosis process. The cytoplasm-located miRNA-21 was first extracted by using the extraction DNA (HP1)-modified P-nanopipette through a hybridization chain reaction (HCR). The nanopipette was then incubated with a labeling DNA (HP2) and reporter 4-MBA-modified Raman tag. The Raman signal (collected from the tip area near the orifice within 1 μm) showed a good response to the content variation of intracellular miRNA-21 under ES, and the proposed single-cell SERS detection platform provides a simple way to study intracellular substance change and evaluate cancer treatment outcomes.
研究领域:
生物技术
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纳米技术
·
癌症研究
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）