FOOD CHEMISTRY
·
2023
·
IF 8.8
Zijian Liang,Pangzhen Zhang,Wen Ma,Xin-An Zeng,Zhongxiang Fang
A new type of flavored wine was produced by macerating either green tea or black tea into Chardonnay wine and model wine respectively, where the physicochemical properties (pH, titratable acidity, color) were modulated. Significant ( p < 0.05) increases of total phenolic content and antioxidant activity (assessed by DPPH, FRAP and ABTS assays) were also observed in the tea macerated wines. A total of 160 phenolic and non-phenolic compounds were identified by HPLC-DAD-ESI-QTOF-MS/MS, where 55 phenolics were newly found in the tea macerated Chardonnay wine. The interaction between wine and tea phenolics led to additional 29 phenolic compounds and 4 non-phenolic compounds that were not found in either Chardonnay wine or tea. Catechin and epigallocatechin gallate were the most abundant phenolic compounds and contributed to the improved antioxidant activities. This study provided a promising prospect of tea as a novel additive in the production of flavored wine with enhanced functionalities.
研究领域:
食品科学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 6
·830-836
·
IF 2.7
Ke Ma,Ze-Yu Su,An-Ran Pei,Xue-Peng Yang
Pyrroloquinoline quinone (PQQ) is a bioactive compound that has attracted significant attention due to its potential health benefits. In this study, we developed a new magnetic molecularly imprinted nanoparticle (MMIN) for the selective extraction and determination of PQQ from food samples. The MMIN was synthesized using a surface molecular imprinting technique with PQQ as the template molecule, Fe3O4 nanoparticles as the magnetic core, and methacrylic acid as the functional monomer. The MMIN exhibited high selectivity and affinity towards PQQ, allowing for efficient extraction and preconcentration of PQQ from complex food matrices. The extracted PQQ was then quantified using HPLC-DAD. The developed method showed good linearity (R2 = 0.9985) and low limits of detection (0.03 μg L−1). The accuracy and precision of the method were evaluated by analyzing spiked food samples, with average recoveries close to 89.8%. The MMIN also demonstrated good reusability, with negligible decrease in extraction efficiency after five cycles of use. Overall, the developed MMIN-based method provides a reliable and efficient approach for the analysis of PQQ in food samples.
研究领域:
食品科学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Yilin Zhang,Mengqi Wu,Jingrui Xi,Chen Pan,Zhizhen Xu,Wei Xia,Wenqing Zhang
In this study, the multiple fingerprints, which were integrated with HPGFC-ELSD (high performance gel filtration chromatography - evaporative light scattering detector) fingerprint, PMP-HPLC-DAD (1-phenyl-3-methyl-5-pyrazolone-high performance liquid chromatography - diode array detector) fingerprint of complete acid hydrolysates and HILIC-HPLC-ELSD (hydrophilic interaction - high performance liquid chromatography - evaporative light scattering detector) fingerprint of enzyme hydrolysates, were established to evaluate the quality of polysaccharides from Poria cocos (PCPs). The similarity evaluation showed that 16 batches of PCPs from different origins had high similarity in structural characteristics based on the multiple fingerprints. The chromatographic data of multiple fingerprints of PCPs were fused, processed and analyzed by chemometric methods including HCA (hierarchical cluster analysis), PCA (principal component analysis) and PLS-DA (partial least squares discriminant analysis). The 16 batches of PCPs were divided into 3 categories in PCA, indicating a certain relationship between the structural characteristics and the origins. PLS-DA analysis indicated that Man, Glc, Gal, Fuc, the components with m/z of 2.22 × 10 4 ∼1.53 × 10 4 Da and 3.46 × 10 3 ∼2.69 × 10 3 Da, oligosaccharides with DPs of 6 and 7, respectively, could be regarded as potential chemical markers for the classification of PCPs from different origins. According to the multiple fingerprints and chemometric analysis, the two commercial samples were proved to be adulterants.
研究领域:
中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2021
·
IF 1.312
Xinyu Wang,Xiaohan Wei,Qingfa Tang,Wei Zhong Huang,Yunxia Teng,Zhizhong Liao,XiaoMei Tan,Xin Mao,Xinyu Wang and Xiaohan Wei are common first authors
Fici Microcarpae Folium, the leaves of Ficus microcarpa, is commonly used for the treatment of influenza, chronic bronchitis, and tonsillitis in Traditional Chinese Medicine (TCM). Ficus microcarpa is mainly distributed in Guangdong and Guangxi provinces. The composition of herbal medicines can be easily affected by geographical regions, the quality control of FMF is essential. In this study, the high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) was developed to obtain a chromatographic fingerprint of Fici Microcarpae Folium from different geographical regions. Meanwhile, the contents of eight compounds of Fici Microcarpae Folium were determined. Principal component analysis (PCA), hierarchical cluster analysis (HCA), and orthogonal partial least squares discrimination analysis (OPLS-DA) were employed to evaluate the HPLC fingerprint. The results showed that the main compositions of Fici Microcarpae Folium from different geographical origins had no difference. The content of the main compositions was different, from which the peak 8, 15, 12, 11, 16, 3, and 21 were the main components that contribute to the difference. Isovitexin, vitexin, and isoorientin were the main components reflecting the quality of Fici Microcarpae Folium. This research could contribute to the quality control of Fici Microcarpae Folium.
研究领域:
中药学
·
分析化学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 23
·7724
·
IF 4.6
Ruixi Gao,Bingchen Han,Yanfeng Zeng,Linchuang Shen,Xinqiao Liu,Qiang Wang,Maochuan Liao,Jun Li
Mosla chinensisMaxim is an annual herb with many potential purposes in agricultural, industrial, and pharmaceutical fields. At present, the extract of the whole plant fromM. chinensishas been proven to demonstrate antifungal, antioxidant, and anti-inflammatory activities. Previous studies focused on the enzyme pretreatment in hydrodistillation fromM. chinensis. However, organic solvent or supercritical fluid carbon dioxide extraction (SFE-CO2) methods, which are commonly utilized in industry, have seldom been studied and cannot provide multiple evaluations of yield. In this work, we analysed compounds fromM. chinensisby HPLC–DAD, discussedn-hexane extraction, and conducted further investigations on SFE-CO2through the design of response surface methodology (RSM). The sample obtained from pilot-scale SFE-CO2was also tested against nine kinds of microorganisms. Single-factor results revealed that the extraction rates fromM. chinensisby steam distillation,n-hexane extraction, and SFE-CO2were 1%, 2.09%, and 3.26%, respectively. RSM results showed a significant improvement in extraction rate through optimising pressure and time, and the interaction of both factors was more important than that of temperature–pressure and temperature–time. A pilot-scale test with an extraction rate of 3.34% indicated that the predicted RSM condition was operable. In addition, samples from the pilot-scale SFE-CO2showed antibacterial effects against three previously unreported bacteria (Gardnerella vaginalis, methicillin-resistantStaphylococcus aureus, andPropionibacterium acnes). These results fill the gap in previous research and provide more information for the application and development ofM. chinensisin the future.Keywords:Mosla chinensisMaxim;HPLC–DAD analysis;SFE-CO2;response surface methodology;antibacterial activity
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Shao Hua Lu,Sha Sha Li,Bo Yin,Jia Ying Mi,Hong Lin Zhai
Diverse interferences often affect the determination of pesticide residues in various kinds of food, and complex pretreatments are necessary. An effective approach for the simultaneous quantitative analysis of multi-target components in different substrates was proposed, and applied to the determination of ternary pesticides in cherry tomatoes and red grape samples. By means of HPLC-DAD, the spectra were obtained under isocratic elution. Utilizing the Tchebichef image moments, unified quantitative models were established for the three target components in the samples with two different substrates, respectively. The correlation coefficients of leave-one-out cross-validation ( R loo - c v 2 ) of the obtained models were more than 0.9975. The predictive correlation coefficients ( R p 2 ) were more than 0.9831. Compared with the N-PLS and MCR-ALS methods, the proposed approach is not only more accurate and reliable, but also can extract essential information and expected to be a potential tool to rapidly quantify multi-component in different substrates without complex pretreatments.
研究领域:
分析化学
·
食品科学
·
化学计量学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Analytical Chemistry
·
2022
·
IF 1.698
Ye Shang,Suyi Liu,Chunxiao Liang,Kunze Du,Shujing Chen,Jin Li,Hua Jin,Yanxu Chang
Pheretima, one of the animal-derived traditional Chinese medicines, has been wildly used in various cardiovascular and cerebrovascular diseases, including stroke, coronary heart disease, hyperlipidemia, and hyperglycemia. However, it was still a big challenge to select the quality markers for Pheretima quality control. The fingerprint and network pharmacology-based strategy was proposed to screen the efficiency related quality markers (Q-Markers) of Pheretima. The ratio of sample to liquid, ultrasonic-extraction time, temperature, and power were optimized by orthogonal design, respectively. The chemical fingerprint of forty batches of Pheretima was established, and six common peaks were screened. The network pharmacology was used to construct the Pheretima-Components-Targets-Pathways-Stroke network. It was found that six potential efficacy Q-markers in Pheretima could exert the relaxing meridians effect to treat stroke through acting on multiple targets and regulating various pathways. A simple HPLC-DAD method was developed and validated to determine the efficacy Q-markers. Grey relational analysis was used to further verify the relation of potential efficiency related quality markers with the anticoagulation activity of Pheretima, which indicated that the contents of these markers exhibited high relationship with the anticoagulation activity. It was concluded that hypoxanthine, uridine, phenylalanine, inosine, guanosine, and tryptophan were selected as quality markers related to relaxing meridians to evaluate the quality of Pheretima. The fingerprint and network pharmacology-based strategy was proved to be a powerful strategy for the discovery of efficiency related Q-markers of Pheretima.
研究领域:
中药学
·
药理学
·
化学计量学
·
质量控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 4
·2689-2696
·
IF 2.6
Jin-Yu Li,Yun-Jiao Ma,Feng Xiao,Xian-Bing Xu
Summary On-site sample preparation has the advantage of combining sampling and sample preparation into a single step, thereby minimising sources of error, and eliminating the risk of analyte instability. Bubble-driven solid-phase microextraction (BD-SPME) was first proposed as an on-site pretreatment method. Oyster sauce underwent on-site pretreatment to measure potassium sorbate levels via high-performance liquid chromatography with a diode array detector (HPLC-DAD), obviating the need for stirrers. The high sensitivity of the HPLC determination of potassium sorbate was obtained using BD-SPME pretreatment with a limit of detection (LOD) of 0.03 mg L −1 and a limit of quantification (LOQ) of 0.10 mg L −1 . The refined technique displayed remarkable linearity from 0.10 to 30.00 mg L −1 , boasting a coefficient of determination R 2 ≥ 0.998. In addition, the recoveries of potassium sorbate in oyster sauce using the method were in the range of 90.8–101.4%. Evidently, BD-SPME in combination with HPLC-DAD is a sensitive and promising analysis for on-site pretreatment.
研究领域:
分析化学
·
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Qilong Xie,Ming Xia,Huaiqian Lu,Hui Shi,Dekui Sun,Bo Hou,Litao Jia,Debao Li
A sample preparation method for the extraction of bisphenol A (BPA) from edible oils was established via simple, green, and sensitive deep eutectic solvent-based liquid-liquid microextraction (DES-LLME) before high-performance liquid chromatography (HPLC) with diode array detector (DAD). The deep eutectic solvent (DES) formed by tetrabutylammonium bromide (TBAB) and methanol was acted as the extractant. All parameters in the DES-LLME step, including the type and volume of extractants, the extraction time, and centrifugation time, were investigated and optimized. The application of hydrophilic DES in oils expands the selection of LLME extractants. Under the optimized conditions, the calibration curves in the range of 10–500 ng g −1 with a correlation coefficient of r > 0.999, and the detection limit was 0.8 ng g −1 . The relative standard deviations of intra-day and inter-day repeatability were 4.3% and 6.2%, respectively, and the recoveries varied between 88.7% and 97.3%. This developed method was successfully employed for measuring BPA in 6 edible oil samples.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Guoyong Xie,Qiuhong Xu,Ran Li,Lu Shi,Yu Han,Yan Zhu,Gang Wu,Minjian Qin
The dried flowers and inflorescences of Buddleja officinalis Maxim are used as traditional medicines in China, and aqueous extracts of the flowers have also been used since ancient times as a yellow rice colorant at local festivals. In this study, HPLC-Q-TOF-MS/MS was used to determine the overall chemical composition of this medicine-food plant. A total of 54 compounds, including 23 flavonoids, 19 phenylethanoid glycosides, 7 alkaloids and 5 other compounds, were detected in the methanol extracts of the herb using this method. Among them, 35 compounds were found firstly in this herb. HPLC fingerprints were also developed, together with a method for the simultaneous quantification of 11 constituents that could be used for quality evaluation of B. officinalis . Fingerprint analysis, using 28 characteristic fingerprint peaks, was used to assess the similarities among 12 samples collected from different geographic areas and showed that the similarity was >0.900. Simultaneous quantification of 11 markers in B. officinalis was then performed to determine consistency of quality. Additionally, the total phenolic content and antioxidant capacity of extracts of the 12 samples of B. officinalis flowers were measured using spectroscopic methods. B. officinalis was found to have good antioxidant capacity and to be a potential natural antioxidant. The highest antioxidant capacity was found in the samples from Guizhou, Sichuan and Guangxi Province. Our results provide valuable information for further understanding and exploiting the herb.
研究领域:
生药学
·
分析化学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2022
·Vol. 38
·期 1
·105-112
·
IF 1.967
Wu Xiaoxiao,Huang Jingjing,Zhang Chi,Kang Xuejun
A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC–DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine with the diazotization products of sulfanilic acid and sodium nitrite in a slightly alkaline medium, followed by NFSPE. Nanofibers comprising polystyrene and zinc acetate were used as a novel solid-phase sorbent to extract derived histidine via the chelation of Zn 2+ with the imidazole group in the analyte. The eluted target compound was analyzed by HPLC–DAD. Good linearity was achieved with the concentrations of histidine ranging from 0.645 to 64.5 µM; the detection limit was 0.193 µM. The intra-day and inter-day coefficients of variation were less than 8.0%. The method was successfully applied to the determination of salivary histidine in the subjects exposed to a public speaking stress. Graphical abstract
研究领域:
分析化学
·
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2861-2870
·
IF 4.58
Qian Liangliang,Li Xiaoqing,Qi Feifei,Li Jian,Lu Lingeng,Xu Qian
An amino-functionalized grooved polyacrylonitrile nanofiber mat was modified by both functional groups and in terms of surface morphology. The mat is shown to represent a viable adsorbent for solid-phase extraction of six priority phenolic pollutants and three phenolic xenoestrogens (phenol, 3-methoxyphenol, 4-nitrophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol, 4-tert-octylphenol, 4-nonylphenol and bisphenol A) from environmental water samples. Under optimized conditions, the analytes were completely extracted from 90 mL of water sample by using only 9 mg of the mat material. Following elution with an ammoniacal methanol-acetone mixture, the eluent was directly submitted to UPLC-MS/MS or HPLC with DAD detection. Satisfied linearity in range of 50 pg·mL −1 to 100 ng·mL −1 was achieved. The detection limits ranged from 4 to 60 pg·mL −1 which revealed the excellent sensitivity of the method in comparison to standardized and other techniques. The recoveries ranged from 82.4% to 111.4%, with the relative standard deviations of 4.0% to 11.4% (for n = 6). The method was applied to the determination of nine phenolic pollutants in water samples collected from sixteen inflow rivers and six Taihu Lake regions.
Amino-functionalized grooved nanofibers mat-based solid-phase extraction for simultaneous determination of nine phenolic pollutants in environmental water
研究领域:
环境科学
·
分析化学
·
纳米技术
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）