ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Xiang-long Tang,Feng Jing,Ben-lan Lin,Sheng Cui,Ru-tong Yu,Xiao-dong Shen,Ting-wei Wang
By overcoming drug resistance and subsequently enhancing the treatment, the combination therapy of photodynamic therapy (PDT) and chemotherapy has promising potential for cancer treatment. However, the major challenge is how to establish an advanced nanoplatform that can be efficiently guided to tumor sites and can then stably release both chemotherapy drugs and a photosensitizer simultaneously and precisely. In this study, which considered the possibility and targeting efficiency of a magnetic targeting strategy, a novel Fe3O4@mSiO2(DOX)@HSA(Ce6) nanoplatform was successfully built; this platform could be employed as an efficient synergistic antitumor nanoplatform with magnetic guidance for highly specific targeting and retention. Doxorubicin (DOX) molecules were loaded into mesoporous silica with high loading capability, and the mesoporous channels were blocked by a polydopamine coating. Human serum albumin (HSA) was conjugated to the outer surface to increase the biocompatibility and blood circulation time, as well as to provide a vehicle for loading photosensitizer chlorin e6 (Ce6). The sustained release of DOX under acidic conditions and the PDT induced by red light exerted a synergistic inhibitory effect on glioma cells. Our experiments demonstrated that the pH-responsive Fe3O4@mSiO2(DOX)@HSA(Ce6) nanoplatform was guided to the tumor region by magnetic targeting and that the nanoplatform suppressed glioma tumor growth efficiently, implying that the system is a highly promising photodynamic therapy/chemotherapy combination nanoplatform with synergistic effects for cancer treatment.
研究领域:
纳米技术
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癌症研究
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生物医学工程
·
药学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Hang Gao,Hang Li,Shuaiqiang Shao,Lintongqing Tan,Yudie Wang,Dawei Li,Wen Zhang,Tao Zhu,Guangchun Liu,Xin Meng
Breast cancer is a malignant tumor that poses a significant threat to women's health and single therapy fails to play a good oncological therapeutic effect. Synergistic treatment with multiple strategies may make up for the deficiencies and has gained widespread attention. In this study, sulfhydryl-modified hyaluronic acid (HA-SH) was covalently crosslinked with polydopamine (PDA) via a Michael addition reaction to develop an injectable hydrogel, in which PDA can be used not only as a matrix but also as a photothermal agent. After HSA and paclitaxel were spontaneously organized into nanoparticles via hydrophobic interaction, hyaluronic acid with low molecular weight was covalently linked to HSA, thus conferring effectively delivery. This photothermal injectable hydrogel incorporates PTX@HSA-HA nanoparticles, thereby initiating a thermochemotherapeutic response to target malignancy. Our results demonstrated that this injectable hydrogel possesses consistent drug delivery capability in a murine breast cancer model, collaborating with photothermal therapy to effectively suppress tumor growth, represented by low expression of Ki-67 and increasing apoptosis. Photothermal therapy (PTT) can effectively stimulate immune response by increasing IL-6 and TNF-α. Notably, the treatment did not elicit any indications of toxicity. This injectable hydrogel holds significant promise as a multifaceted therapeutic agent that integrates photothermal and chemotherapeutic modalities.
研究领域:
肿瘤学
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dazhuang Liu,Shuna Mi,Shaofeng Yuan,Hang Yu,Yahui Guo,Yuliang Cheng,Weirong Yao
This study developed a human serum albumin-functionalized gold core-shell nanocomposite (Au@HSA) to address limitations of commercial AuNPs in detecting sodium pentachlorophenol (NaPCP) and ibuprofen (IBU) via surface-enhanced Raman scattering (SERS). Molecular docking and multispectral analyses revealed HSA-induced structural transitions (α-helix to β-sheet) enhancing substrate stability and analyte adsorption. Optimized detection parameters (HSA: 1 μg/mL; pH 7 for NaPCP, pH 9 for IBU; 30–55 min incubation; Au@HSA-to-analyte ratios 2:1–5:2) enabled linear detection of both analytes (0.5–100 mg/L) with limits of 0.32 mg/L (NaPCP) and 0.27 mg/L (IBU). Validations in tap water and pears demonstrated 97.10 %–102.84 % recovery rates and ≤ 4.64 % RSD, confirming practical applicability. The Au@HSA platform overcomes commercial AuNP limitations by leveraging HSA's molecular recognition and stabilization properties, offering enhanced sensitivity for dual-pollutant monitoring in environmental and food samples. This work provides a robust solution for detecting toxic pesticides and pharmaceutical pollutants while advancing functional nanocomposite design for SERS-based sensing.
研究领域:
分析化学
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纳米技术
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环境科学
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药学
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生物传感
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jianjun Du,Tao Zhu,Quanyong Gu,Wenbing Cao,Jiangli Fan,Xiaojun Peng
Green fluorescent protein (GFP) becomes one of the most important imaging tools in biology and biomedicine. To simplify usage, expand the application and avoid complicated operations, a kind of artificial fluorescent protein (AFP) is inspired and fabricated by lighting up human serum albumin (HSA) with loading microenvironment-sensitive fluorescent dye DMAR , which could be applied in HSA assay in human urine and serum samples as a physiological indicator of diagnosis in clinical. Besides, the AFP, DMAR /HSA, could act as a nanosensor for Ag + sensing with good selectivity by a new relay recognition strategy.
研究领域:
生物化学
·
医学诊断
·
分子生物学
✓ 已验证
DOI
高影响因子
Toxins
·
2025
·Vol. 18
·期 1
·7
·
IF 4
Cheng Chen,Lu Chen,Hongyuan Zhou,Xiao Li Shen,Liang Ma
α-Zearalenol (α-ZOL), the primary metabolite of zearalenone (ZEN), is a prevalent mycotoxin in agricultural products (e.g., corn, wheat) and poses health risks due to its toxicity. However, strategies to mitigate its toxicity are needed. Therefore, this study aims to determine whether selected polyphenols (quercetin, baicalin, rosmarinic acid, naringenin) can competitively displace α-ZOL from human serum albumin (HSA) and to clarify the interaction mechanisms. The results showed that competitive interactions between α-ZOL, HSA, and the polyphenols were observed. The polyphenols bound HSA more tightly than α-ZOL (higherKa) and significantly reduced α-ZOL’sKa, indicating direct competition. Moreover, as evidenced by synchronous fluorescence, the polyphenols altered the microenvironments of tyrosine and tryptophan residues, directly impacting α-ZOL binding. The HPLC-ultrafiltration results revealed that the polyphenols tested competitively displaced α-ZOL from HSA, with the relative potency of quercetin ≈ baicalin > rosmarinic acid > naringenin. Collectively, our competitive binding assays demonstrate that quercetin, baicalin, rosmarinic acid, and naringenin competitively displace α-ZOL from its binding site(s) on HSA. Thus, our study not only suggests a novel mechanism to alleviate the toxicity of ZEN and α-ZOL but also provides a scientific basis for developing dietary interventions against these mycotoxins.
研究领域:
毒理学
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食品安全
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分子营养学
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生物化学
·
真菌毒素研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-19
·
IF 12.6
Huang Yuelin,Lu Zhenghai,Liu Huanhuan,Yang Ningxin,Chen Yanhong,Wang Chunting,Huang Weixi,Hu Jingjing,Wang Dengbin,Yao Defan
Background Breast cancer remains the most common malignancy among women worldwide. While traditional computed tomography (CT) scans and image-guided radiotherapy are widely used for breast cancer diagnosis and treatment, their efficacy is often limited. Methods and results In this study, we successfully synthesized human serum albumin (HSA)-based KBiF 4 nanoclusters through a simple one-pot biomimetic mineralization strategy. Compared to the clinical contrast agent iohexol, KBiF 4 @HSA significantly enhances dual-energy CT (DECT) imaging contrast at high keV levels, offering improved diagnostic accuracy for breast cancer. Furthermore, KBiF 4 @HSA exhibits a remarkable ability to scavenge elevated glutathione (GSH) levels and promote reactive oxygen species (ROS) generation. When combined with radiotherapy, KBiF 4 @HSA substantially increases X-ray dose deposition at tumor sites, leading to enhanced DNA damage and suppression of breast cancer progression. Importantly, KBiF 4 @HSA demonstrates excellent biocompatibility in vivo, with no significant tissue damage or inflammation observed. Conclusions This study presents a novel approach for the development of biocompatible DECT contrast agents and radiosensitizers, offering a promising strategy to enhance breast cancer diagnosis and treatment. However, the efficacy of this approach needs to be further validated across diverse breast cancer subtypes to ensure its broad applicability, which emphasizes the necessity for continued research to fully translate this innovative technology into clinical practice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Fei Xu,YuQing Shen,ZhiQiang Pan,Xuan Zhou,Wei Gu,Jie Dong,Shaoping Yin,ShengJin Liu,Ming Xu,Baoduan Chen
The interactions between human serum albumin (HSA) and the hemostatic components of the Chinese medicine Sanguisorbae Radix (SR), specifically phenolic acid compounds such as caffeic acid (CA), ferulic acid (FA) and their 1:1 mixture (1:1) were studied to investigate the molecular mechanism underlying the hemostatic effect of SR. Network pharmacology combined with the experimental and computational data revealed that HSA is one of the hemostatic targets to SR phenolic acids. SDS-PAGE and multi-spectroscopy demonstrated that the phenolic acids bind to the Sudlow site I on HSA, altering its structure and influencing its migration velocity. There is an observed synergistic effect upon the mixture of CA and FA. Quantum chemistry, molecular docking, and molecular dynamics simulations indicate that the binding of phenolic acids to HSA is stable, and variations in binding efficiency are associated with the hydrophobicity of the substituent at the C3 position of the side chain, and also, the key amino acids and functional groups for hemostasis of SR were identified, along with the active sites that contribute to the synergistic enhancement by phenolic acids.
研究领域:
药理学
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生物化学
·
计算生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR RECOGNITION
·
2024
·Vol. 37
·期 2
·e3075
·
IF 2.3
Xinluan Lv,Wenjin Li,Miao Zhang,Ruiyong Wang,Junbiao Chang
The binding of four alkaloids with human serum albumin (HSA) was investigated by isothermal titration calorimetry (ITC), spectroscopy and molecular docking techniques. The findings demonstrated that theophylline or caffeine can bind to HAS, respectively. The number of binding sites and binding constants are obtained. The binding mode is a static quenching process. The effects of steric hindrance, temperature, salt concentration and buffer solution on the binding indicated that theophylline and HSA have higher binding affinity than caffeine. The fluorescence and ITC results showed that the interaction between HSA and theophylline or caffeine is an entropy-driven spontaneous exothermic process. The hydrophobic force was the primary driving factor. The experimental results were consistent with the molecular docking data. Based on the molecular structures of the four alkaloids, steric hindrance might be a major factor in the binding between HSA and these four alkaloids. This study elucidates the mechanism of interactions between four alkaloids and HSA.
研究领域:
生物化学
·
药物化学
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产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2025
·Vol. 17
·期 3
·316
·
IF 5.5
Yang Chen,Shuang Liang,Binglin Chen,Fei Jiao,Xuliang Deng,Xinyu Liu
Background/Objectives : Paclitaxel (PTX) is a potent anticancer drug that is poorly soluble in water. To enhance its delivery efficiency in aqueous environments, amphiphilic polymer micelles are often used as nanocarriers for PTX in clinical settings. However, the hydrophilic polymer segments on the surface of these micelles may possess potential immunogenicity, posing risks in clinical applications. To address this issue, nanomicelles based on human serum albumin (HSA)–hydrophobic polymer conjugates constructed via site-specific in situ polymerization-induced self-assembly (SI-PISA) are considered a promising alternative. The HSA shell not only ensures good biocompatibility but also enhances cellular uptake because of endogenous albumin trafficking pathways. Moreover, compared to traditional methods of creating protein–hydrophobic polymer conjugates, SI-PISA demonstrates higher reaction efficiency and better preservation of protein functionality. Methods : We synthesized HSA-PMEMA nanomicelles via SI-PISA using HSA and methoxyethyl methacrylate (MEMA)—a novel hydrophobic monomer with a well-defined and stable chemical structure. The protein activity and the PTX intracellular delivery efficiency of HSA-PMEMA nanomicelles were evaluated. Results : The CD spectra of HSA and HSA-PMEMA exhibited similar shapes, and the relative esterase-like activity of HSA-PMEMA was 94% that of unmodified HSA. Flow cytometry results showed that Cy7 fluorescence intensity in cells treated with HSA-PMEMA-Cy7 was approximately 1.35 times that in cells treated with HSA-Cy7; meanwhile, HPLC results indicated that, under the same conditions, the PTX loading per unit protein mass on HSA-PMEMA was approximately 1.43 times that of HSA. These collectively contributed to a 1.78-fold overall PTX intracellular delivery efficiency of HSA-PMEMA compared to that of HSA. Conclusions : In comparison with HSA, HSA-PMEMA nanomicelles exhibit improved cellular uptake and higher loading efficiency for PTX, effectively promoting the intracellular delivery of PTX. Tremendous potential lies in these micelles for developing safer and more efficient next-generation PTX formulations for tumor treatment.
研究领域:
药剂学
·
生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aging-US
·
2021
·
IF 5.682
Anlong Jiang,Nanxiang Wang,Xinxing Yan,Yunheng Jiang,Chengchao Song,Hui Chi,Guanghua Chen,Feng Wu,Ye Ji,Jinglong Yan
Ossification of the posterior longitudinal ligament (OPLL) is a disorder with multiple pathogenic mechanisms and leads to different degrees of neurological symptoms. Recent studies have revealed that non-coding RNA (ncRNA), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), could influence the development of OPLL. Nevertheless, the molecular mechanisms linking circular RNAs (circRNAs) and the progression of OPLL is still unknown. The current research explored the expression profiles of OPLL-related circRNAs by microarray analysis, and applied qRT-PCR to validate the results. Subsequently, we confirmed the upregulation of hsa_circ_0007292 in OPLL cells by qRT-PCR and validated the circular characteristic of hsa_circ_0007292 by Sanger sequencing. Fluorescence in situ hybridization (FISH) unveiled that hsa_circ_0007292 was predominantly located in the cytoplasm. Functionally, gain-of-function and loss-of-function experiments showed that hsa_circ_0007292 promoted the osteogenic differentiation of OPLL cells. Mechanistically, the interaction of hsa_circ_0007292 and miR-508-3p was predicted and validated by bioinformatics analysis, dual-luciferase reporter assays, and Ago2 RNA immunoprecipitation (RIP). Similarly, we validated the correlation between miR-508-3p and SATB2. Furthermore, rescue experiments were performed to prove that hsa_circ_0007292 acted as a sponge for miR-508-3p, and SATB2 was revealed to be the target gene of miR-508-3p. In conclusion, our research shows that hsa_circ_0007292 regulates OPLL progression by the miR-508-3p/SATB2 pathway. Our results indicate that hsa_circ_0007292 can be used as a promising therapeutic target for patients with OPLL.
研究领域:
分子生物学
·
遗传学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Peng Zhang,Xinjie Guo,Yuzhe Xiao,Qian Zhang,Caifeng Ding
Accurate detection of human serum albumin (HSA) in biological samples is quite meaningful for early disease diagnosis and treatment. Herein, a novel fluorescent probe 1-ethyl-4-[2-[4-(diethylamino)-2-hydroxyphenyl]ethenyl]]-pyridinium salt (DEHP) was developed for HSA determination. The inherent fluorescence of DEHP is essentially negligible at physiological conditions assigned to the well-developed twisted intramolecular charge transfer (TICT) protocol. An intriguing fluorescence light up is triggered as the addition of HSA, on account of the inhibited TICT procedure when DEHP enters the hydrophobic cavity of protein HSA. This combination leads to a turn on fluorescent response for HSA with a detection limit of 4.8 nM. After an overall investigation, it has been proved that the strong binding between DEHP and HSA is specific-site-related. In additional, the probe implies a great potential to assist clinical diagnosis due to the usage in actual serum detection. Cell imaging also shows that the probe is expected to monitor HSA production process at cell level.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
临床诊断
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Shiman Lu,Zhaoqing Shi,Chendi Ding,Wenyan Wang,Weilang Zhang,Li Huang,Jiachan Lin,Xinpei Wang,Ran Luo,Xiaowei Zeng,Wenhu Zhou,Hongzhong Chen,Qiangsong Wang,Lin Mei
The targeted protein degradation (TPD) strategy modulates tumor growth pathways by degrading proteins of interest (POIs) and has reshaped anti-tumor drug research and development. Recently, the emergence of photodegradation-targeting chimeras (PDTACs) and laser irradiation at specific sites enables precise spatiotemporal controllability of TPD. Capitalizing on the advances of PDTACs, herein, we report a nanoplatform for efficiently delivering PDTAC molecule for photodegradation of bromodomain-containing protein 4 (BRD4) proteins, the key activators of oncogenic transcription. The PDTAC molecule, named as PPa-JQ1, is synthesized through the covalent attachment of the BRD4-targeting ligand JQ1-acid, to the photosensitizer pyropheophorbide-a (PPa), utilizing a 1,6-hexanediamine linker. The PPa-JQ1 is further encapsulated by human serum albumin (HSA) to obtain the HSA@PPa-JQ1 nanoplatform, which facilitates targeted and efficacious delivery to melanoma lesions. Both in vitro and in vivo therapeutic outcomes demonstrate that HSA@PPa-JQ1 can efficiently generate reactive oxygen species (ROS) to degrade BRD4 upon light irradiation, which eventually induces tumor death. Our study represents the first case to validate the anti-tumor therapeutic efficacy of PDTACs by systemic administration, providing the foundation for further application of PDTACs.
研究领域:
肿瘤学
·
药学
·
纳米技术
·
光化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）