Food Bioscience
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2024
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IF 4.8
Qing Liu,Yan Song,Ruonan Wang,Jinwei Sun,Jian He,Qiming Li,Guofang Zhang,Xinkai Ma,Chun Li,Libo Liu
Helicobacter pylori ( H. pylori ) resistance due to antibiotics is becoming increasingly prevalent, and the search for healthy alternatives to drugs has become a popular research topic. Curcuma longa and Lactobacillus rhamnosus are not only inhibitory to H. pylori but also have good compatibility. It was found that acid-tolerant L. rhamnosus had a significant coaggregation effect on H. pylori , but the inhibitory effect was weak. The polyphenol or flavonoid content of Curcuma extract was significantly correlated with the antimicrobial effect, but the inhibition time would be shortened due to its decomposition or destabilization in the digestive system. Both were able to synergistically inhibit H. pylori adhesion and expression of certain genes ( SabA , UreE , UreI , and UreG ), with no significant synergistic effect in inhibiting urease. The combination of Curcuma extract with L. rhamnosus enhanced the inhibitory effect, solved the problem of drug resistance, and improved the short duration of inhibition of Curcuma extract. This study aimed to combine the antimicrobial effect of Curcuma extract with the function of L. rhamnosus in regulating the gastrointestinal flora and to investigate the synergistic inhibitory effect of the two on H. pylori . The findings provide a theoretical basis for subsequent cellular, animal, or clinical experiments.
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Acta Pharmaceutica Sinica B
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2024
·
IF 14.7
Yongkang Lai,Tinglin Zhang,Xiaojing Yin,Chunping Zhu,Yiqi Du,Zhaoshen Li,Jie Gao
Helicobacter pylori ( H. pylori) infection remains the leading cause of gastric adenocarcinoma, and its eradication primarily relies on the prolonged and intensive use of two antibiotics. However, antibiotic resistance has become a compelling health issue, leading to H. pylori eradication treatment failure worldwide. Additionally, the powerlessness of antibiotics against biofilms, as well as intracellular H. pylori and the long-term damage of antibiotics to the intestinal microbiota, have also created an urgent demand for antibiotic-free approaches. Herein, we describe an antibiotic-free, multifunctional copper-organic framework (HKUST-1) platform encased in a lipid layer comprising phosphatidic acid (PA), rhamnolipid (RHL), and cholesterol (CHOL), enveloped in chitosan (CS), and loaded in an ascorbyl palmitate (AP) hydrogel: AP@CS@Lip@HKUST-1. This platform targets inflammatory sites where H. pylori aggregates through electrostatic attraction. Then, hydrolysis by matrix metalloproteinases (MMPs) releases CS-encased nanoparticles, disrupting bacterial urease activity and membrane integrity. Additionally, RHL disperses biofilms, while PA promotes lysosomal acidification and activates host autophagy, enabling clearance of intracellular H. pylori . Furthermore, AP@CS@Lip@HKUST-1 alleviates inflammation and enhances mucosal repair through delayed Cu 2+ release while preserving the intestinal microbiota. Collectively, this platform presents an advanced therapeutic strategy for eradicating persistent H. pylori infection without inducing drug resistance.
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Small
·
2025
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IF 12.1
Ke Wang,Shiru Sun,Xiaobing Tang,Honghong Jiao,Jingyu Weng,Hongbo Xu,Shuming Li,Ming Lv
Helicobacter pylori is a pathogen that can withstand the pH levels in the stomach and cause gastric infections. It is widely recognized as a major risk factor for gastric diseases, and its eradication could effectively treat peptic ulcers and gastritis, and reduce the mortality rate of gastric cancer. However, the antibiotic combination therapies typically used to eradicate H. pylori can disrupt the gut microbiota. Therefore, this study presents a gastrointestinal environment intelligent-responsive nanozyme (CeOSL) for treatment of multidrug-resistant H. pylori infections and regulation of inflammation. The oxidase-like activity of CeOSL is activated in the gastric juice and mucus, which generates superoxide radicals that eradicate H. pylori . Furthermore, CeOSL can scavenge reactive oxygen species to alleviate inflammation in the gastric epithelium with no significant effect on symbiotic microbiota, which addresses the pathological conditions associated with H. pylori infections. This pH-modulated nanozyme approach provides a safer and more effective strategy for treatment of H. pylori infections.
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RSC Advances
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2018
·Vol. 8
·期 38
·21075-21083
·
IF 2.936
Li-Li Chen,Hui-Fang Cui,Shuang-Fei Fan,Zong-Yi Li,Shuang-Yin Han,Xin Ma,Shu-Wen Luo,Xiaojie Song,Qi-Yan Lv
Noninvasive diagnosis of Helicobacter pylori (H. pylori) infection is very attractive. This study investigated the single strand DNA (ssDNA) acquisition method from H. pylori in dental plaque, and the integration of our previously developed 43-mer H. pylori DNA biosensor with the obtained target ssDNA (tDNA). Dental plaque samples were collected from 34 patients/volunteers, whose gastric H. pylori infection statuses were tested with the 13C urea breath test (UBT). The samples were treated with colony polymerase chain reaction (PCR) to obtain double strand DNA (dsDNA) of 104 basepairs (bp) long. A blocker ssDNA was designed and used in thermal treatment of the dsDNA to release the 104-mer tDNA, which contains the 43-mer DNA sequence in the middle. PCR primers were designed, and the tDNA releasing and detection conditions with the biosensor were optimized. The limit of detection with the biosensor was 12 fM dsDNA. The dental plaque detection results correlated quite well with the UBT results, with a sensitivity of 100%, and specificity of 97%. These results indicate that the residence of H. pylori in dental plaque is highly associated with gastric H. pylori infection, and detection of dental plaque samples with our DNA biosensor is promisingly applicable in noninvasive diagnosis of H. pylori infection.
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JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
Yukun Lin,Kunjing Liu,Jiahui Chen,Tingjie Li,Zijin Sun,Yifan Fan,Peng Zou,Yanting Liu,Chunyu Wang,Chongyang Ma,Xueqian Wang,Fang Lu,Fafeng Cheng,Changming Zhai
A B S T R A C T Ethnopharmacological relevance Pinellia ternata (Ban-xia) and Coptidis Rhizoma (Huang-lian) form a classic herb pair (BXHL) in traditional Chinese medicine that has demonstrated clinical efficacy against Helicobacter pylori ( H. pylori ) infection. However, its pharmacological mechanisms remain insufficiently defined. Aim of the study This study aimed to (1) evaluate the anti- H. pylori colonization effects of BXHL in vitro and in vivo; (2) elucidate its mechanism through the NLRP3-mediated pyroptosis pathway; and (3) assess its influence on gastrointestinal microbiota and identify potential active components. Materials and methods BXHL composition was characterized by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Anti- H. pylori activity was examined using GES-1 cell co-culture and infected mouse models. Gene expression and signaling pathways were analyzed by RNA-seq, ELISA, RT-qPCR, Western blotting and IHC. Histopathology, bacterial load assays, and ultrastructural observation assessed anti-colonization efficacy. Microbiota alterations were analyzed via 16S rRNA sequencing and molecular docking evaluated interactions between BXHL compounds and NLRP3 targets. Results BXHL significantly reduced H. pylori adhesion, colonization and restored NLRP3-mediated pyroptosis suppressed by infection. Transcriptomic and molecular analyses confirmed reactivation of the NLRP3 signaling cascade. BXHL preserved gut microbial diversity, unlike triple therapy, and exhibited moderate direct antibacterial effects. Conclusion BXHL inhibits H. pylori colonization through suppressing bacterial adhesion factors and activating NLRP3-mediated pyroptosis, while causing no disruption to the gut microbiota. These findings position BXHL as a safe and effective adjunctive therapy, which provides a novel strategy to improve H. pylori eradication that complements conventional antibiotics.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Muhammad Arif,Min He,N.A. Samreen,Hasi Hays,Ling Wang,Xiyuan Zhang,Bingqiang Zhang,Guotai Li,Tengbo Yu,Qihui Zhou
The increasing prevalence of antibiotic-resistant Helicobacter pylori (R- H. pylori ) poses a significant clinical challenge, contributing to persistent gastritis, peptic ulcers, and gastric cancer. Conventional antibiotic therapies are increasingly limited by resistance, incomplete eradication, and disruption of the gut microbiota. To address these challenges, we developed pH-responsive iron-doped ammonium citrate carbon dots (Fe-CDs), a nanozyme with intrinsic specificity for targeting and eradicating R- H. pylori within the acidic gastric microenvironment. Fe-CDs exhibit dual oxidase (OXD)- and peroxidase (POD)-like catalytic activities that are selectively activated under acidic conditions, driving the localized generation of reactive oxygen species (ROS) via Fenton and Fenton-like reactions involving Fe(II) and endogenous hydrogen peroxide (H₂O₂). The nanozyme shows strong binding affinity for H. pylori through ammonium (NH 4 + )-mediated interactions and specifically targets Urel, thereby inhibiting urease activity and disrupting bacterial acid resistance. This dual mechanism—acid-enhanced ROS production combined with urease suppression—confers potent bactericidal effects against R- H. pylori while preserving commensal microbiota and minimizing off-target damage. Transcriptomic analysis of infected gastric tissue revealed that Fe-CDs induce ROS-mediated stress pathways and metabolic disruption in H. pylori . In vivo studies confirmed that Fe-CDs significantly reduce bacterial load in R- H. pylori -infected mice with negligible toxicity. These findings highlight Fe-CDs as a targeted, antibiotic-free therapeutic strategy for resistant gastric infections and offer a promising alternative to conventional treatments.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Dantong Zhou,Zhanlin Zhang,Bo Qiu,Dandan Zhang,Shuang Xie,Kun Huang,Xiaohong Li
Helicobacter pylori (H. pylori) is recognized as a pathogenic factor related to gastrointestinal diseases and gastric cancer. The theranostics of H. pylori infection is confronted with challenges from colonization on the epithelial cell layer and formation of bacterial biofilms, which prevent interactions with imaging probes and antimicrobial agents. Herein, theranostic nanoparticles (NPs) are developed with ultrasonication (US)-propelled motion and US-excited persistent luminescence for precise imaging and efficient eradication of H. pylori. Mechanoluminescent SrAl2O4:Eu2+ (SAOE) and persistently luminescent ZnGa2O4:Cr3+ (ZGC) nanodots (NDs) were in situ deposited on mesoporous silica NPs to prepare mSZ NPs. mSZ-Clr@ePDA NPs were fabricated through surface adsorption of clarithromycin (CLR) and then coating with enzyme-responsive polydopamine layers (ePDA). US-activated SAOE NDs generate emissions to excite ZGC NDs for persistent emission of near-infrared (NIR) afterglows, but they are quenched by the ePDA coatings. The disintegration of ePDA layers by bacterial phospholipase rapidly restores NIR emissions for imaging of H. pylori biofilms and simultaneously triggers the CLR release. US-driven motion promotes NP penetration and CLR delivery across mucus into biofilms, leading to full destruction of the biofilm matrix and embedded bacteria. In an H. pylori-infection model, mSZ-Clr@ePDA/US treatment enhances the retention of NPs in the stomach and penetration across mucus and accumulation on the epithelial layer, and the US-activated persistent emission provides a real-time diagnosis of H. pylori infection without autofluorescence. The US-propelled accumulation of mSZ-Clr@ePDA NPs and local release of CLR in biofilms significantly eliminate bacterial burdens in gastric tissues at a lower dose of antibiotics. Thus, this study offers a feasible strategy to activate persistent emission in deep tissues and provides a potential theranostic solution for non-invasive imaging and efficient removal of H. pylori infection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinyuan Zhang,Yongkang Lai,Liang Zhang,Zheng Chen,Jiulong Zhao,Shige Wang,Zhaoshen Li
Helicobacter pylori ( H. pylori ) is one of the major causes of gastrointestinal diseases, including gastric cancer. However, the acidic environment of the stomach and H. pylori resistance severely impair the antimicrobial efficacy of oral drugs. Here, a biocompatible chitosan-modified molybdenum selenide (MoSe 2 @CS) was designed for the simultaneous photothermal treatment of H. pylori infection and gastric cancer. MoSe 2 @CS showed a photothermal conversion efficiency was as high as 45.7 %. In the H. pylori -infected mice model, MoSe 2 @CS displayed a high bacteriostasis ratio of 99.9 % upon near-infrared irradiation. The antimicrobial functionality was also proved by transcriptomic sequencing study, which showed that MoSe 2 @CS combined with NIR laser irradiation modulated the gene expression of a variety of H. pylori bioprocesses, including cell proliferation and inflammation-related pathways. Further gut flora analysis results indicated that MoSe 2 @CS mediated PTT of H. pylori did not affect the homeostasis of gut flora, which highlights its advantages over traditional antibiotic therapy. In addition, MoSe 2 @CS exhibited a good photothermal ablation effect and significantly inhibited gastric tumor growth in vitro and in vivo . The comprehensive application of MoSe 2 @CS in the PTT of H. pylori infection and gastric cancer provides a new avenue for the clinical treatment of H. pylori infection and related diseases.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-19
·
IF 14.7
Liu Tao,Chai Shuang,Li Mingyang,Chen Xu,Xie Yutao,Zhao Zehui,Xie Jingjing,Yu Yunpeng,Gao Feng,Zhu Feng,Yang Lihua
Infection by Helicobacter pylori , a prevalent global pathogen, currently requires antibiotic-based treatments, which often lead to antimicrobial resistance and gut microbiota dysbiosis. Here, we develop a non-antibiotic approach using sonodynamic therapy mediated by a lecithin bilayer-coated poly(lactic-co-glycolic) nanoparticle preloaded with verteporfin, Ver-PLGA@Lecithin, in conjunction with localized ultrasound exposure of a dosage permissible for ultrasound medical devices. This study reveals dual functionality of Ver-PLGA@Lecithin. It effectively neutralizes vacuolating cytotoxin A, a key virulence factor secreted by H. pylori , even in the absence of ultrasound. When coupled with ultrasound exposure, it inactivates H. pylori by generating reactive oxygen species, offering a potential solution to overcome antimicrobial resistance. In female mouse models bearing H. pylori infection, this sonodynamic therapy performs comparably to the standard triple therapy in reducing gastric infection. Significantly, unlike the antibiotic treatments, the sonodynamic therapy does not negatively disrupt gut microbiota, with the only major impact being upregulation of Lactobacillus , which is a bacterium widely used in yogurt products and probiotics. This study presents a promising alternative to the current antibiotic-based therapies for H. pylori infection, offering a reduced risk of antimicrobial resistance and minimal disturbance to the gut microbiota.
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BIOORGANIC & MEDICINAL CHEMISTRY
·
2009
·
IF 3.075
Wen-Jun Mao,Peng-Cheng Lv,Lei Shi,Huan-Qiu Li,Hai-Liang Zhu
Fourteen metronidazole derivatives (compounds 3a – f and 4b – h ) have been synthesized by coupling of metronidazole and salicylic acid derivatives. All of them are reported for the first time. Their chemical structures are characterized by 1 H NMR, MS, and elemental analysis. The inhibitory activities against Helicobacter pylori urease have been investigated in vitro and many compounds have showed promising potential inhibitory activities of H. pylori urease. The effect of compounds 4b (IC 50 = 26 μM) and 4g (IC 50 = 12 μM) was comparable with that of acetohydroxamic acid, a well known H. pylori urease inhibitor used as a positive control. The experimental values of IC 50 showed that inhibitor was potent urease inhibitor. A docking analysis using the autodock 4.0 program could explain the inhibitory activities of compound 4g against H. pylori urease.
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Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Xiaojuan You,Mingyi Shao,Huadong Wang,Rui Zhu,Xinwei Liu,Lei Dong,Yuesheng Gong,Yongwei Li
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of H. pylori in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by in-situ reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and H. pylori was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect H. pylori in a wide concentration range (101 CFU mL−1–108 CFU mL−1) with a low detection limit of 3 CFU mL−1. What’s more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Puji Peng,Feng Xu,Yuying Xu,Shuang Sun
This study presented the determination of Helicobacter pylori using a gold electrode (AuE)-based electrochemical biosensor in which the electroactive label was β-cyclodextrin (β-CD). The covalent immobilization of a thiol-decorated single-stranded DNA probe on the AuE surface occurred through the formation of a Au–S bond. The DNA hybridization was monitored via the differential pulse voltammetry (DPV) method, where the electrochemical signals for reduction of the β-CD bound to the double-stranded DNA (dsDNA) were measured. The electrochemical signal was linearly related to the target DNA concentration (0.3 nM–0.24 μM) when measured under optimal conditions, and the limit of detection (LOD) was determined to be as low as 0.15 nM, suggesting that measurements with our developed biosensor were highly repeatable and reproducible. In addition, our proposed biosensor was successfully applied to the determination of Helicobacter pylori in excrement.
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