BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Wang Ren,Ying Zhang,Wei Tao Huang,Nian Bing Li,Hong Qun Luo
This work reported a label-free colorimetric assay for sensitive detection of Hg 2+ based on Hg 2+ -triggered hairpin DNA probe (H-DNA) termini-binding and exonuclease Ш (Exo Ш)-assisted target recycling, as well as hemin/G-quadruplex (DNAzyme) signal amplification . The specific binding of free Hg 2+ with the thymine–thymine (T–T) mismatches termini of H-DNA could immediately trigger the Exo Ш digestion, and then set free G-quadruplex segments and Hg 2+ . The Exo Ш impellent recycling of ultratrace Hg 2+ produced numerous G-quadruplexes. The corresponding DNAzymes catalyzed efficiently the H 2 O 2 -mediated oxidation of the ABTS 2− to the colored product in the presence of hemin . Using the color change as the output signal, and the Exo Ш-aided Hg 2+ recycling and DNAzyme as the signal amplifier, the ultrasensitive assay system successfully achieved visual detection of Hg 2+ as low as 1.0 nM by the naked eye, and was suitable for field monitoring. The calibration curve was linear in the range of 50.0 pM to 20.0 nM for Hg 2+ ( R =0.9962) with a detection limit of 10.0 pM. Moreover, this proposed strategy showed excellent selectivity , portability and low-cost, and was successfully applied to colorimetric detection of Hg 2+ in laboratory tap water and Jialing river water samples.
研究领域:
分析化学
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环境科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
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2016
·
IF 2.693
Zhenhu Song,Fang Wang,Jian Qiang,Zhijie Zhang,Yahui Chen,Yong Wang,Wei Zhang,Xiaoqiang Chen
We developed a simple and sensitive hydrogel sensor in the form of microspheres by using fluorescence probe encapsulated within a hydrogel matrix for the detection of Hg 2+ . The traditional fluorescence probes suspended in solution are not transportable and recoverable. To overcome these disadvantages, we devised poly(ethylene glycol) diacrylate-based hydrogel microspheres in which fluorescence probe ( R19S ) was embedded at high density. The functionalized hydrogel microspheres were prepared by combining a microfluidic device with UV light . The hydrogel microspheres-based sensor exhibited good selectivity to Hg 2+ among various metal ions and high sensitivity with a detection limit of 90 nM. Furthermore, after binding with Hg 2+ , the R19S encapsulated hydrogel microspheres can be separated from testing samples easily and treated with the solution containing KI to remove Hg 2+ and realize reusable detection. The current work may offer a new method for Hg 2+ recognition with a more efficient manner.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2012
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IF 0.633
Yan Gao,Jian Song,Wei Wang
Four novel azobenzene derivatives, 4,4'-bis(CH2SAr)azobenzene (Ar = different heterocyclic units), have been prepared and characterised by IR, 1H NMR, 13C NMR, MS and elemental analysis. Their complexation properties to different heavy and transition metal ions have been studied by UV and fluorescence spectra. The compound 4,4'-bis(4,6-dimethyl-pyrimidine-2-ylmethylthio)azobenzene shows selective recognition of Hg2+.
研究领域:
化学
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分析化学
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材料科学
✓ 已验证
DOI
高影响因子
Environmental Science-Nano
·
2021
·Vol. 8
·期 12
·3833-3845
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IF 8.131
Tingting Li,Yangsai Lyu,Jia Li,Chengyan Wang,Ningning Xing,Jie Yang,Min Zuo
Micromotors have held great promise for environmental treatment due to their unique self-propelled movement that can significantly accelerate the chemical process in solution. Herein, we have developed a new AO–Mn2O3/γ-AlO(OH) fluorescent micromotor using renewable and uniquely hollow kapok fibers as biological templates. The AO–Mn2O3/γ-AlO(OH) micromotor was powered by the asymmetric decomposition of hydrogen peroxide fuel catalyzed by Mn2O3, reaching a maximum speed of 139.6 μm s−1 in 3% H2O2 solution. The micromotor revealed a markedly enhanced fluorescence effect compared to both pure AO solution and non-micromotor counterparts and exhibited excellent selectivity and sensitivity for Hg2+ detection. The detection range and limit for Hg2+ were 5 × 10−8–5 × 10−5 M and 2.25 × 10−8 M, respectively. The enhanced Hg2+ sensing benefited from the synergy of enhanced fluorescence via the inhibition of the dimerization of AO, fluorescence quenching induced by charge transfer and the enhanced contact between the micromotor and Hg2+ ions resulted from the self-propelled motion. This micromotor-assisted fluorescence detection idea provided a new strategy for the development of sensors with high sensitivity.
研究领域:
环境科学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-11
·
IF 5.3
Zhu Liqin,Lou Congcong,Zhang Xiaomei,Yang Fei
Porphyrin-based porous organic polymer (POP) with uniformly immobilized platinum nanoparticles (Pt NPs) were designed and synthesized, and it was demonstrated that such nanocomposites (Pt/POP) have oxidase-like activity. Surprisingly, Hg 2+ significantly enhanced the oxidase-like activity of Pt/POP. The enhancement was attributed to the capture of Hg 2+ by the thioether group in Pt/POP and the subsequent redox reaction of Hg 2+ with Pt NPs, accelerating the electron transfer. In the presence of Hg 2+ , Pt/POP catalyzed the colorless 3,3',5,5'-tetramethylbenzidine (TMB) to turn blue rapidly and changed its absorbance at 652 nm. Based on this, a fast-response colorimetric sensor was constructed for the sensitive detection of Hg 2+ with a linear range of 0.2–50 μM and a detection limit of 36.5 nM. Importantly, Pt/POP can be used as an adsorbent for the efficient removal of Hg 2+ with a removal efficiency as high as 99.4%. This work provides a valuable strategy for colorimetric detection and efficient removal of Hg 2+ . Graphical abstract
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 11
·900
·
IF 3.504
Longshi Rao,Yong Tang,Hanguang Lu,Shudong Yu,Xinrui Ding,Ke Xu,Zongtao Li,Jin Z. Zhang
We developed a microreactor with porous copper fibers for synthesizing nitrogen-doped carbon dots (N-CDs) with a high stability and photoluminescence (PL) quantum yield (QY). By optimizing synthesis conditions, including the reaction temperature, flow rate, ethylenediamine dosage, and porosity of copper fibers, the N-CDs with a high PL QY of 73% were achieved. The PL QY of N-CDs was two times higher with copper fibers than without. The interrelations between the copper fibers with different porosities and the N-CDs were investigated using X-ray photoelectron spectroscopy (XPS) and Fourier Transform infrared spectroscopy (FTIR). The results demonstrate that the elemental contents and surface functional groups of N-CDs are significantly influenced by the porosity of copper fibers. The N-CDs can be used to effectively and selectively detect Hg2+ions with a good linear response in the 0~50 μM Hg2+ions concentration range, and the lowest limit of detection (LOD) is 2.54 nM, suggesting that the N-CDs have great potential for applications in the fields of environmental and hazard detection. Further studies reveal that the differentdorbital energy levels of Hg2+compared to those of other metal ions can affect the efficiency of electron transfer and thereby result in their different response in fluorescence quenching towards N-CDs.Keywords:microreactor;carbon dots;porous copper fibers;Hg2+detection
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Shao-Bin He,Quan-Quan Zhuang,Liu Yang,Mei-Ying Lin,Ye Kuang,Hua-Ping Peng,Hao-Hua Deng,Xing-Hua Xia,Wei Chen
A visual assay for the detection of heparinase was developed on the basis of a ternary system of Hg2+–heparin–osmium nanoparticles (OsNPs). First, heparin-capped OsNPs (heparin–OsNPs) were synthesized by a facile reduction method using heparin as the protecting/stabilizing agent. The oxidase-like activity of heparin–OsNPs, however, turned out to be low, which somewhat limits their application. We discovered that Hg2+ can significantly/specifically boost the oxidase-like activity of heparin–OsNPs via electrostatic interaction. The oxidase-like activity of heparin–OsNPs toward the oxidation of the substrate, 3,3′,5,5′-tetramethylbenzidine, by dissolved O2 was found to increase by 76-fold in the presence of Hg2+. More significantly, heparin in heparin–OsNPs could be specifically hydrolyzed into small fragments in the presence of heparinase, which resulted in the weakening of the oxidase-like activity of Hg2+/heparin–OsNPs. On the basis of these findings, a linear response of the sensor for heparinase was obtained in the range 20–1000 μg/L with a low detection limit (15 μg/L), which is comparable to those of other reported sensors. Further, the colorimetric sensor was employed for the detection of heparinase in human serum samples with satisfactory results. We speculate that combining such surface modification of the osmium nanozyme with a sensing element could be an interesting direction for promoting nanozyme research in medical diagnosis.
研究领域:
生物化学
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医学诊断
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纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Daifang Wang,Longhua Guo,Rong Huang,Bin Qiu,Zhenyu Lin,Guonan Chen
Electrochemiluminescence (ECL) of Ru(bpy) 3 2+ has been widely used in analytical chemistry . Herein, we present a new approach to enhance the ECL of Ru(bpy) 3 2+ by the localized surface plasmon resonance (LSPR) of gold nanorods (AuNR). Our investigations reveal that the ECL intensity could be greatly enhanced by controlling the distance between Ru(bpy) 3 2+ and the surface of AuNRs. We call this kind of surface plasmon induced ECL enhancement as surface-enhanced electrochemiluminescence (SEECL). This kind of SEECL phenomenon is utilized to fabricate a biosensor for ultrasensitive Hg 2+ detection. The SEECL biosensor is fabricated by self-assembling AuNRs and T-rich ssDNA probes on gold electrode surface. With the presence of Hg 2+ , the conformation of ssDNA probes changed to be hairpin-like structure via formation of T-Hg 2+ -T structure. And Ru(bpy) 3 2+ could insert into the grooves of the hairpin structured DNA probes to generate ECL emission, which could be enhanced by the LSPR of AuNR. The ECL intensity of the sensor increased with the concentration of Hg 2+ , and a detection limit of 10 fM Hg 2+ in aqueous solution was achieved. The effect of different LSPR peak location of AuNR on the sensitivity of biosensor has been investigated. The results show that a good overlap between the LSPR absorption spectrum and the ECL emission spectrum of Ru(bpy) 3 2+ could achieve the best ECL signal enhancement.
研究领域:
分析化学
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生物传感
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 43
·16388-16396
·
IF 8.067
Wenrui Ma,Lulu Liu,Xu Zhang,Xingfei Liu,Yi Xu,Shunbo Li,Muling Zeng
Artificial membranes with asymmetric nanopores are similar to biological membranes with ion channels. The selective and rectified ion transport property of artificial membranes is of great significance for developing electrochemical sensors with highly sensitive detection performance. Herein, we propose a fast and facile approach for the sensitive detection of metal ions or charged molecules by constructing an asymmetric nanochannel structure using a cation-selective Nafion membrane and a DNA-functionalized anodic aluminum oxide (AAO) membrane. Arising from the varying charge distributions induced by the recognition of targets by DNA probes, the Nafion/AAO heterostructured nanochannels exhibit high ionic current rectification and excellent Hg2+ regulated current responses. The present sensor with heterostructured nanochannels can realize the detection of Hg2+ with a low detection limit of 1 pM, due to the unique ionic transport property of asymmetric nanochannels and the sensitive electrochemical detection method. The sensing mechanism is also confirmed by numerical simulations, demonstrating that the differences in the rectification ratios in the heterostructured nanochannels are caused by the varying surface charge densities in AAO nanochannels. This simple and facile heterostructured sensor has the advantages of simple preparation, easy modification, simple instrumentation and high sensitivity, which possesses great potential in the detection of metal ions or charged molecules.
研究领域:
生物传感器
·
电化学检测
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纳米技术
·
分析化学
✓ 已验证
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2017
·Vol. 28
·期 11
·1418-1427
·
IF 1.907
Like Zhang,Yanan Wang,Jiajia Huang,Siguo Yuan
Herein, we report the synthesis and adsorption property of a novel chelating fiber containing azido group. Firstly, the brominated fiber (PP-St-DVB-Br) was obtained via the reaction of polypropylene-(g)-styrene-divinylbenzene fiber (PP-St-DVB) with bromine in CH 2 Cl 2 solution. Then, azido chelating fiber (PP-St-DVB-N 3 ) was prepared by azidation of PP-St-DVB-Br fiber. Its structure and properties were characterized by Fourier transform infrared, elemental analysis, thermogravimetric analysis, and chemical titration, respectively. The micromophology and functional group distribution in fibrous matrix were investigated by scanning electron microscopy-energy dispersive spectroscopy. The results show that the chelating fiber has high functional group contents (2.11 mmol/g for PP-St-DVB-N 3 ) and uniform distribution. Different from granulate chelating resin, the novel fibrous adsorbent possesses excellent adsorption ability for Hg(II) and Pb(II) ions (408.9 mg/g for Hg 2+ and 334.4 mg/g for Pb 2+ ), and the adsorption capacity of the fiber has no loss until five cycles. The novel absorbent material shows the potential application prospect in the treatment of heavy metal wastewater. Copyright © 2017 John Wiley & Sons, Ltd.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Chunqing Li,Li Xiao,Qinyu Zhang,Xinjian Cheng
It is urgent and important to detect heavy metals in environments. In this work, novel reaction-based fluorescent probes were obtained by Schiff base reaction. The probes with Schiff base moiety (-C=N-) undergo irreversible hydrolysis in the presence of Hg 2+ and Fe 3+ . They exhibit perfect high selectivity and sensitivity to Hg 2+ and Fe 3+ ions. Upon the addition of Hg 2+ and Fe 3+ , fluorescence intensity of the probes increased notably. And the color of the probe changes from brown to bright green under UV light, which can realize “naked eye” detection. In addition, Schiff base group was introduced into polyurethane chain through condensation polymerization reaction . As expected, the fluorescent polyurethane probe (P2) maintained the detection performance of its original small molecules (BSD). Even more P2 showed a more sensitive detection effect than BSD, and the detection limits of P2 for Hg 2+ and Fe 3+ reach 0.19 μM and 0.21 μM, respectively. It indicates that Reaction-based probes could be a useful tool for the detection of Hg 2+ and Fe 3+ .
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2019
·Vol. 21
·期 6
·2919-2928
·
IF 3.567
Kang-Tao Liu,Shi-Yong Ran
The metal ion–DNA interaction is key to biochemical processes and has applications in areas such as metal ion sensors and DNA nanomachines. For example, the formation of the T–Hg2+–T structure has been used in technologies such as DNA-based mercuric ion sensors. Though the interaction is widely used for practical purposes, the underlying mechanism has not been fully understood. In the present study, we used magnetic tweezers to explore the interactions between λ-DNA and two metal ions, Hg2+ and Cd2+, at the single-molecule level. Both metal ions caused considerable DNA conformational changes. The resulting DNA compaction dynamics were related to the ion concentration and the exerted force. The increase in the ion concentration promoted DNA compaction, whereas exerting greater forces inhibited this process. Application of a high force generated two-stage dynamics of the Hg2+–DNA interaction. However, at a sufficiently high Hg2+ concentration, a lower force led to a three-stage process. In contrast, the curves of the binding of Cd2+ ions to DNA had a stepwise pattern. Both the AFM scanning results and the single-molecule measurements confirmed that Hg2+ influences the DNA conformation in a more pronounced manner than Cd2+. The multistage Hg2+–DNA interaction was considered to be a result of the different binding mechanisms, including the mismatched base-pair formation. A model was then proposed to explain the peculiar dynamics.
研究领域:
生物物理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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