Cell Discovery
·
2025
·Vol. 11
·期 1
·1-14
·
IF 12.5
Wang Jinrui,Niu Shenghui,Hu Xiao,Li Tianxing,Liu Shengduo,Tu Yingfeng,Shang Zehua,Zhao Lin,Xu Pinglong,Lin Jingwen,Chen Lu,Billadeau Daniel D.,Jia Da
The cGAS-STING pathway mediates the innate immune response to cytosolic DNA, contributing to surveillance against microbial invasion or cellular damage. Once activated, STING recruits TBK1 at the trans-Golgi network (TGN), which in turn phosphorylates IRF3 to induce type I interferon (IFN-I) expression. In contrast to STING, little is known about how TBK1 is transported to the TGN for activation. Here, we show that multiple TGN tethering factors, a group of proteins involved in vesicle capturing, are indispensable for STING-IFN-I signaling. Deletion of TBC1D23, a recently reported tethering factor, in mice impairs the STING-IFN-I signaling, but with insignificant effect on STING-NF-κB signaling. Mechanistically, TBC1D23 interacts with TBK1 via the WASH complex subunit FAM21 and promotes its endosome-to-TGN translocation. Furthermore, multiple TGN tethering factors were reduced in aged mice and senescent fibroblasts. In summary, our study uncovers that TGN tethering factors are key regulators of the STING-IFN-I signaling and suggests that their reduction in senescence may produce aberrant STING signaling.
研究领域:
免疫学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
He Xiaoyang,Lin Li,Li Xiangying,Zhu Minzhi,Zhang Qinghong,Xie Shunji,Mei Bingbao,Sun Fanfei,Jiang Zheng,Cheng Jun,Wang Ye
The membrane electrode assembly (MEA) is promising for practical applications of the electrocatalytic CO 2 reduction reaction (CO 2 RR) to multi-carbon (C 2+ ) compounds. Water management is crucial in the MEA electrolyser without catholyte, but few studies have clarified whether the co-feeding water in cathode can enhance C 2+ formation. Here, we report our discovery of pivotal roles of a suitable nanocomposite electrocatalyst with abundant Cu 2 O−Cu 0 interfaces in accomplishing water-promoting effect on C 2+ formation, achieving a current density of 1.0 A cm −2 and a 19% single-pass C 2+ yield at 80% C 2+ Faradaic efficiency in MEA. The operando characterizations confirm the co-existence of Cu + with Cu 0 during CO 2 RR at ampere-level current densities. Our studies reveal that Cu + works for water activation and aids C‒C coupling by enhancing formations of adsorbed CO and CHO species. This work offers a strategy to boost CO 2 RR to C 2+ compounds in industrial-relevant MEA by combining water management and electrocatalyst design.
研究领域:
材料科学
·
化学工程
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DOI
PubMed
高影响因子
MUTAGENESIS
·
2025
·
IF 4.3
Hu Yushi,Lei Yuyang,Gao Zhenna,Cao Yiyi,Xi Jing,Ma Yuning,Gao Qinwen,Fu Jian,Zhang Xinyu,Luan Yang
Aristolochic acid I (AAI) is a carcinogen associated with various human cancers. However, its causal relationship with hepatocellular carcinoma remains controversial, and inconsistent results from rodent studies have suggested species-specific differences. Here we evaluated AAI genotoxicity using functional human-induced hepatocyte-like cells (hiHep cells), a model that closely mimics primary human hepatocytes in gene expression and function, thereby shedding light on its potential hepatocarcinogenic risk in humans. First, we assessed AAI genotoxicity by evaluating AAI-DNA adducts and micronucleus frequency. In hiHep cells, AAI (0.7–2.5 µM) induced up to 105 adducts per 108 nucleotides, indicating high metabolic activation of AAI. A concentration-dependent increase in micronucleus frequency indicated a significant increase in chromosomal aberrations in hiHep cells. Considering the evidence of AAI inducing oxidative stress, we assessed 8-hydroxy-2′-deoxyguanosine and reactive oxygen species levels to evaluate DNA oxidative damage. For both indicators, significantly elevated levels were observed. A mechanism involving oxidative damage was further supported by observations of mitochondrial dysfunction, including changes in mitochondrial membrane potential and mitochondrial complex activity. Ascorbate treatment decreased AAI-induced oxidative DNA damage and DNA adduct formation, providing direct cellular evidence for free radical intermediates in AAI metabolic activation—a mechanism previously hypothesized but not experimentally validated in a human-relevant hepatocyte model. Our study findings revealed the genotoxic effects of AAI on hiHep cells and implicated oxidative stress as the key mechanism. These findings strengthen the association between AAI exposure and liver disease and highlight the potential role of antioxidant therapies in mitigating AAI-associated carcinogenesis.
研究领域:
毒理学
·
肝脏生物学
·
癌症研究
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Huihui Yang,Jun Chen,Yuling Liang,Yanfei Zhang,Wen Yin,Yuzhi Xu,Si-Yang Liu,Zong Dai,Xiaoyong Zou
Dynamic imaging of cell surface pH is extremely challenging due to the slight changes in pH and the fast diffusion of secreted acid to the extracellular environment. In this work, we construct a novel metal–organic framework (MOF)-shell-confined i-motif-based pH probe (MOFC-i) strategy that enables sensitive and dynamic imaging of cell surface pH. The CY3- and CY5-labeled i-motif, which is hybridized via its short complementary chain with two-base mismatches, is optimized for sensing at physiological pH. After efficiently anchoring the optimized pH probes onto the cell membrane with the aid of cholesterol groups, a biocompatible microporous MOF shell is then formed around the cell by cross-linking ZIF-8 nanoparticles via tannic acid. The microporous MOF shell can confine secreted acid without inhibiting the normal physiological activities of cells; thus, the MOFC-i strategy can be used to monitor dynamic changes in the cell surface pH of living cells. Furthermore, this method can not only clearly distinguish the different metabolic behaviors of cancer cells and normal cells but also reveal drug effects on the cell surface pH or metabolism, providing promising prospects in pH-related diagnostics and drug screening.
研究领域:
生物化学
·
细胞生物学
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分子生物学
·
生物医学工程
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DOI
PubMed
高影响因子
LANGMUIR
·
2021
·
IF 3.882
Hui Yuan,Hsiu-Wei Cheng,Laura LE Mears,Renliang Huang,Rongxin Su,Wei Qi,Zhimin He,Markus Valtiner
Osteoarthritis is a prevalent degenerative joint disease characterized by progressive articular cartilage loss and destruction. The resultant increase in friction causes severe pain. The collagen I matrix (COL I) has been used clinically for cartilage repair; however, how COL I acts at cartilage surfaces is unclear. Here, we studied adsorption and lubrication of synovial fluid components, albumin, γ-globulin, and the phospholipid DPPC, on COL I under physiological conditions using surface plasmon resonance and an in situ sensing surface force apparatus. Our results revealed COL I had poor lubrication ability, a fairly high coefficient of friction (COF, μ = 0.651 ± 0.013), and surface damage under a 7 mN load. DPPC formed an improved lubricating layer on COL I (μ = 0.072 ± 0.016). In sharp contrast, albumin and γ-globulin exhibited poor lubrication with an order of magnitude higher COF but still provided benefits by protecting COL I from wear. Hence, DPPC on COL I may help optimize COL I implantation design.
研究领域:
生物医学工程
·
骨科研究
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 11
·6605-6614
·
IF 3.108
Ce Gao,Qingda An,Zuoyi Xiao,Shangru Zhai
Nowadays, processing of ultra-low sulfur fuel oil has been a hot topic of research all over the world; hence, an efficient adsorption process to tackle this issue is eliciting increasing attention. However, the preparation of high-performance adsorbents is still a challenge. Herein, zirconium-substituted mesoporous SBA-15 with highly dispersed active Cu(I) species has been successfully synthesized by our group at 493 K via a mild vapor-reduction method. The as-prepared material was characterized by powder X-ray diffraction (XRD), nitrogen adsorption and desorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and UV-vis diffuse reflectance (DRUV) spectroscopy. All characterization methods demonstrated that the Cu(I) species were highly dispersed in an ordered mesoporous framework with short channels rather than piled on the surface. The as-prepared materials also exhibited an excellent adsorption capacity for thiophene (up to 29.86 mg g−1 at 323 K). Pseudo-second-order kinetics model and Freundlich isotherm can well describe the adsorption kinetics and isotherm data, respectively. More remarkably, an ultrasound-assisted treatment can be effectively employed to regenerate adsorbent, with only a slight reduction in the adsorption capacity after five cycles.
研究领域:
材料科学
·
能源化学
·
环境工程
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DOI
高影响因子
Small
·
2023
·Vol. 19
·期 49
·2304644
·
IF 13.3
Ping Lu,Zhaoyang Qi,Jie Chen,Changshen Ye,Ting Qiu
High-efficiency adsorption of aromatic sulfur-containing compounds from liquid hydrocarbon fuels over metal-organic frameworks (MOFs) is challenging because of inert metal sites. A new method, the Ce-enhanced modulation of MOFs’ microenvironment, is proposed to modulate the –COO···Cu(II)– coordination microenvironment of Hong Kong University of Science and Technology (HKUST-1) using Ce(III) as a molecular scalpel for fabricating abundant high-efficiency Cu(I) and Cu-coordination-unsaturated sites and improving the pore structures around adsorptive sites. The optimal CH-250 thus exhibits adsorptive capacities for 20.2, 28.0, and 58.3 mg S g –1 of thiophene, benzo-thiophene, and dibenzothiophene, respectively, which are superior to most reported MOFs, zeolites, and nanoporous carbons. The constructed Cu(I) sites show stronger affinity for dibenzothiophene (−0.86 eV) than the initial Cu(II) (−0.74 eV) for out-of-plane adsorption. Further, they are far stronger in-plane adsorption interactions in DBT/CH-250 (−0.90 eV) than those in DBT/HKUST-1 (−0.37 eV). Thus, molecular engineering for modulating the coordination microenvironment of MOFs shows great potential for adsorption desulfurization.
研究领域:
材料科学
·
能源化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Yu-Feng Zhu,Yong-Sheng Wang,Bin Zhou,Yan-Qin Huang,Xue-Jiao Li,Si-Han Chen,Xiao-Feng Wang,Xian Tang
We for the first time confirmed that the low concentrations of Ag(I) could induce a silver specific aptamer probe (SAP) from a random coil sequence form to G-quadruplex structure. Thereby, a novel highly sensitive fluorescence strategy for silver(I) assay was established. The designed multifunctional SAP could act as a recognition element for Ag(I) and a signal reporter. The use of such a SAP can ultrasensitively and selectively detect Ag(I), giving a detection limit down to 0.64 nM. This is much lower than those reported by related literatures. This strategy has been applied successfully for the detection of Ag(I) in real samples, further proving its reliability. Taken together, the designed SAP is not only a useful recognition and signal probe for silver, but also gives a platform to study the interaction of monovalent cations with DNA .
研究领域:
分析化学
·
生物化学
·
纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Sai-Li Zheng,Yonghe He,Xiao-Kang Qiu,Yuan-Hui Zhong,Lai-Hon Chung,Wei-Ming Liao,Jun He
A novel metal-organic framework [CuCN(TMBP)·H 2 O] n ( TCuCN ) has been synthesized by the reaction of 3,3′,5,5′-tetramethyl-4,4′-bipyrazole (TMBP) and CuCN. Its structure was determined by single-crystal X-ray diffraction (SCXRD) analysis and further characterized by power X-ray diffraction (PXRD) analysis, FT-IR spectroscopy and thermogravimetric (TG) analysis. Previously reported structurally analogous TCuCl , TCuBr , TCuI have also been synthesized for Br 2 adsorption comparison. All of these four MOFs exhibited reversible uptake of Br 2 vapor judging by the unchanged PXRD patterns . Among four MOFs, Br 2 -loaded TCuI showed the most uptake amount of Br 2 (15.7 wt%), providing the formula to be TCuI ·0.33Br 2 . The different uptake amount of Br 2 for TCuX (X = CN, Cl, Br, I) should be mainly ascribed to the intermolecular interaction between CuX and Br 2 .
研究领域:
无机化学
·
材料化学
·
配位化学
·
多孔材料
·
气体吸附
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DOI
高影响因子
POLYMER SCIENCE SERIES B
·
2021
·Vol. 63
·期 3
·272-279
·
IF 1.049
Liangliang Yang,Liu Fang,Zhao Qun
Abstract The antibacterial properties of chitosan and self-repairing properties of collagen-I play important roles in the medical field. New types of collagen-I/chitosan/methyl vinyl silicone rubber materials have been synthesized by blending and grafting copolymerization. The effects of chitosan contents on mechanical and antibacterial properties of collagen-I/chitosan/methyl vinyl silicone rubber were investigated, while the morphology and molecular structure were observed and analyzed. It was determined that the antibacterial and self-repairing properties of collagen-I/chitosan/methyl vinyl silicone rubber were better than those of methyl vinyl silicone rubber. Additionally, the optimal contents of chitosan and collagen-I were determined to be 5 wt % and 5 mg/mL, respectively.
研究领域:
生物医学材料
·
高分子化学
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DOI
高影响因子
INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Zhenzhong Wang,Yiping Du,Chao Wang,Lin Ma,Chen Li,Taifeng Lin,Jiawen Xiao,Zhengguang Yan
Hybrid copper(I) halides have garnered a significant amount of attention as potential substitutes in luminescence and scintillation applications. Herein, we report the discovery and crystal growth of new zero-dimensional compounds, (C2H8N)3Cu2I5 and (C2H8N)4Cu2Br6. The bromide and iodide have a triclinic structure with space group P1̅ and an orthorhombic structure with space group Pnma, respectively. (C2H8N)3Cu2I5 exhibits cyan emission peaking at 504 nm with a photoluminescence quantum yield (PLQY) of 34.79%, while (C2H8N)4Cu2Br6 shows yellowish-green emission peaking at 537 nm with a PLQY of 38.45%. The temperature-dependent photoluminescence data of both compounds were fitted to theoretical models, revealing that nonradiative intermediate states significantly affect thermal quenching and antiquenching. Electron–phonon interactions, the origin of emission line width broadening and peak shifting, were also investigated via fittings. The scintillation properties of (C2H8N)3Cu2I5 were evaluated, and an X-ray imaging device was successfully fabricated using (C2H8N)3Cu2I5. This work demonstrates the potentiality of copper halides in lighting and X-ray imaging applications.
研究领域:
材料科学
·
物理化学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Xionghua Sun,Xuelei Wan,Li Chen,Yadong Cui,Xihua Chen,Jian Zhang,Xiaogang Jiang
The senescence of alveolar epithelial cells is one of the characteristics of mitomycin pulmonary toxicity, but the direct target of mitomycin-induced alveolar epithelial cell senescence remains unclear. In this study, a method of small-molecule ligand–protein interaction by pulldown/mass spectrometry identified peroxiredoxin I (Prdx I) as the target of mitomycin-induced alveolar epithelial cell senescence, and mitomycin was shown to significantly inhibit the peroxidase activity of Prdx I. Consequently, the levels of cellular reactive oxygen species (ROS) increased, leading to the activation of mitogen-activated protein kinase kinase 1/2 (MEK1/2)-extracellular signal-regulated protein kinases 1/2 (ERK 1/2) and, accordingly, to the increased expression of p53. Both a Prdx I activator and Prdx I overexpression alleviated mitomycin-induced alveolar cell senescence. A ROS scavenger inhibited MEK1/2-ERK1/2-p53 activation and then attenuated the senescence of alveolar epithelial cells triggered by mitomycin. Thus, Prdx I can serve as the first target of mitomycin-induced alveolar epithelial cell senescence, which might represent a promising approach to reduce mitomycin-induced lung toxicity.
研究领域:
药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
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