Frontiers in Bioengineering and Biotechnology
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2022
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IF 6.064
Zhou Mengyang,Guo Min,Shi Xincui,Ma Jie,Wang Shutao,Wu Shuo,Yan Weiqun,Wu Feng,Zhang Peibiao
Multifunctionality has becoming essential for bone tissue engineering materials, such as drug release. In this study, icariin (ICA)-incorporated poly(glycolide-co-caprolactone) (PGCL) porous microcarriers were fabricated and then coated with decellularized extracellular matrix (dECM) which was derived from bone marrow mesenchymal stem cells (BMSC). The porous structure was generated due to the soluble gelatin within the microcarriers. The initial released ICA in microcarriers regulated osteogenic ECM production by BMSCs during ECM formation. The dECM could further synergistically enhance the migration and osteogenic differentiation of BMSCs together with ICA as indicated by the transwell migration assay, ALP and ARS staining, as well as gene and protein expression. Furthermore, in vivo results also showed that dECM and ICA exhibited excellent synergistic effects in repairing rat calvarial defects. These findings suggest that the porous microcarriers loaded with ICA and dECM coatings have great potential in the field of bone tissue engineering.
研究领域:
组织工程
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生物材料
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干细胞生物学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Neuropsychiatric Disease and Treatment
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2022
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IF 2.989
Ting Zhang,Keli Dong,Lan Xiao,Guangcheng Li,Zhanwei Zhang
Objective We investigated the effect of icariin (ICA) combined with Panax notoginseng saponins (PNS) on intestinal microbiota and hippocampal protein expression in amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice, a model of Alzheimer’s disease (AD).Methods Transgenic mice were treated with icariin and PNS. The Morris water maze (MWM) was used to assess spatial memory, and the gut microbiota and differential protein expression in the hippocampus were investigated using high-throughput screening techniques. Differential protein expression was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting.Results The MWM results showed that the mice treated with the medium dose of ICA+PNS spent significantly more time in the target quadrant compared with the AD group. Bacterial diversity was the lowest in the AD group, with significantly greater diversity in the ICA + PNS treatment group. Three proteins were selected for proteomic analysis, and qRT-PCR and Western blot were used to detect the expression of 2ʹ-5ʹ-oligoadenylate synthetase ubiquitin like 1 (Oasl1), trichoplein keratin filament-binding protein (TCHP), and tumor necrosis factor receptor associated 3-interacting protein 1 (MIPT3). Compared with control mice, MIPT3 expression was increased and Oasl1 and TCHP were reduced in the AD group. These abnormal protein expressions tended to normalization after treatment with medium dose of ICA and PNS.Conclusion Treatment with ICA and PNS ameliorated memory impairment in an AD mouse model. The mechanisms may be related to modulation of the intestinal microbiota and expression of Oasl1, TCHP, and MIPT3.
研究领域:
神经科学
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药理学
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微生物学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
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2021
·Vol. 5
·期 1
·91-103
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IF 5.693
Zhu Tengyang,Zhao Xiaoxu,Yi Ming,Xu Sheng,Wang Yan
To eliminate the interfacial defects between polymeric matrix and inorganic fillers in hybrid membranes, various strategies have been proposed by modifying membrane materials or optimizing membrane formation process. In this work, a ternary cross-linked PVA-APTES-ZIF-90 (PVA, poly(vinyl alcohol); APTES, 3-aminopropyltriethoxysilane; ZIF-90, zeolitic imidazolate framework-90) membrane was developed to eliminate interfacial defects between PVA and ZIF-90 for enhanced ethanol dehydration performance, where APTES served as a covalently linked bridge to cross-link PVA and ZIF-90 simultaneously. The morphologies and physicochemical properties of the resultant membranes were characterized by various characterization techniques. Moreover, we also explored the effects of [ZIF-90]/[APTES] mole ratio and [ZIF-90]/[APTES] loading on the separation performance of the trinity membranes. The results indicated that ZIF-90 particles showed better dispersion and compatibility in PVA-APTES-ZIF-90 trinity membranes than in PVA/ZIF-90 hybrid membrane, attributed to the ternary cross-linking among PVA, APTES, and ZIF-90. Compared with pristine PVA membrane, PVA-APTES-ZIF-90 trinity membranes showed stronger anti-swelling and mechanical properties, as well as more excellent separation performance, which resulted from the incorporation of cross-linker APTES and ZIF-90 particles. The separation factor of the optimal PVA-APTES-ZIF-90 trinity membrane was improved by 215.4%, up to 552, accompanied by a total flux of 82 g/m 2 h. Ternary cross-linking among cross-linker, inorganic filler, and polymeric chain is therefore believed to be a feasible strategy in preparing polymeric-based hybrid membranes with high separation performance. Graphical abstract Summary: A novel ternary cross-linked hybrid membrane with APTES as co-cross-linkers of ZIF-90 and PVA for efficient ethanol dehydration
研究领域:
材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
He Qi,Zheng Li,Haijiao Zheng,Qiong Jia
Protein phosphorylation regulates the conformations and function of proteins, which plays an important part in organisms. However, systematic and in-depth analysis of phosphorylation often hinders on account of the low abundance and suppressed ionization of phosphopeptides. Various materials based on single enrichment mechanism show potential in phosphopeptides enrichment, but the enrichment performance is typically not satisfactory. Herein, we developed a carnosine (Car) functionalized magnetic metal organic framework designed as Fe 3 O 4 @NH 2 @ZIF-90@Car. Benefiting from the multiple recognition groups of Car and massive metal ions site of ZIF-90, the as-fabricated Fe 3 O 4 @NH 2 @ZIF-90@Car was utilized as a multifunctional material with synergistic effect for phosphopeptides enrichment. On the basis of combined immobilized metal ion affinity chromatography (IMAC) and amine-based affinity enrichment mechanism, Fe 3 O 4 @NH 2 @ZIF-90@Car exhibited higher enrichment performance of phosphopeptides compared with Fe 3 O 4 @NH 2 @ZIF-90 (single IMAC mechanism). Besides, the feasibility of Fe 3 O 4 @NH 2 @ZIF-90@Car nanocomposites in complicated samples was further verified by enriching phosphopeptides from nonfat milk, human fluids such as serum and saliva, demonstrating its bright application prospects in phosphoproteomics analysis.
研究领域:
生物化学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2021
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IF 9.078
Kun Li,Yingnan Zhang,Junwei Xu,Jingxi Wang,Xuenan Gu,Ping Li,Yubo Fan
The electrospun fibrous scaffold has shown a great potential due to an extracellular matrix-mimicking structure of nanofibers , however, a three-dimensional (3D) fibrous scaffold, much similar to in vivo environment, still remains challenging in fabrication. The magnetic nanoparticles (MNPs) have been explored to promote bone-related cells activity under static magnetic field (SMF). Herein, via electrospinning, Fe 3 O 4 MNPs and icariin (ICA) from traditional Chinese medicine were introduced into polycaprolactone (PCL) fibers to manufacture two-dimensional (2D) membranes (PCL/Fe 3 O 4 /ICA), which were then expanded to 3D scaffold by depressurization of subcritical CO 2 fluid. The expanding behavior was more remarkable for the membrane collected from rotary collector than that on plate collector, especially after the addition of Fe 3 O 4 MNPs. Co-cultured with pre-osteoblasts, PCL/Fe 3 O 4 /ICA 3D scaffold induced a higher cell proliferation viability than that in 2D membrane in later period, and the combined utilization with SMF group showed the highest cell viability. When implanted subcutaneously, 3D scaffold exhibited better cell infiltration , internal collagen deposition and angiogenesis due to the enhanced porosity and the action of ICA. This highly porous magnetic PCL/Fe 3 O 4 /ICA 3D scaffold provided a new idea for the design and application of magnetic scaffold in the bone tissue engineering .
研究领域:
材料科学
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组织工程
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生物材料
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生物医学工程
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
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2023
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IF 6.6
Xuan Chen,Qiang Ren,Jingmei Xu,Mengchi Ju,Lingxin Meng,Zhe Wang
In this paper, two kinds of metal-organic frameworks (MOFs) with proton conduction capability were successfully prepared and added into sulfonated poly (ether ketone sulfone) (C-SPAEKS) by organic-inorganic blending. Then two different series of proton exchange membranes (PEMs) were fabricated. The template used in MOFs was UiO-66-NH 2 (UN), by introducing sulfonic acid groups and imidazole groups on the template, additional proton sources and proton transport sites were constructed. Such multifunctional MOFs contributed greatly to the proton transport efficiency and structure-activity relationship of C-SPAEKS. The sulfonic acid functionalized MOFs (USN), sulfonic-acid-imidazole bifunctional MOFs (IUSN) and composite PEMs were characterized by XRD, XPS, FT-IR, 1 H NMR and SEM. The prepared PEMs all have excellent proton conductivity, in which the proton conductivity of C-SPAEKS/IUSN-3% could reach 0.234 S cm −1 (80 °C and 100% relative humidity). Nafion117 had a conductivity of 0.105 S cm −1 (80 °C and 100% relative humidity), so C-SPAEKS/IUSN-3% exhibited a great enhancement on proton conductivity. Moreover, the oxidation stability of C-SPAEKS/IUSN-3% membrane was 96.97%, which was 39.32% higher than that of C-SPAEKS. These results all indicated that the as-prepared MOFs have made great contribution to improving the comprehensive properties of the sulfonated poly (aryl ether ketone) proton exchange membrane.
研究领域:
材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Wenfu Lin,Yan Huang,Zhenru Li,Jiongshen Pan,Tiansheng Huang,Lili Liang,Yanzhen Tan,Jie Li,Li-Peng Sun,Bai-Ou Guan
Humidity plays an important role in many fields, and the realization of high sensitivity and fast response simultaneously for humidity detection is a great challenge in practical application. In this work, we demonstrated a high-performance relative humidity (RH) sensor made by supporting zeolitic imidazolate framework-90 (ZIF-90)-derived porous zinc oxide (ZnO) onto an optical microfiber Sagnac interferometer (OMSI). The ZIF-90-modified OMSI (ZIF-90-OMSI) sensor was in situ heated at different temperatures to obtain porous ZnO, and their humidity-sensing properties were investigated ranging from 25 to 80% RH. The experimental results showed that the porous ZnO fiber sensor prepared at 500 °C (Z500-OMSI) exhibited best humidity-sensing performance with a high sensitivity of 96.2 pm/% RH (25–45% RH) and 521 pm/% RH (50–80% RH) and ultrafast response/recovery time (62.37/206.67 ms) at 22.3% RH. These performances were attributed to the complete transformation of ZIF-90 to ZnO at 500 °C. The obtained Z500 not only retained the high porosity and specific surface area of ZIF-90 but also exhibited the exceptional hydrophilicity of ZnO. In addition, the signals of the proposed Z500-OMSI sensor changed with different breathing patterns, indicating the possibility for human respiration monitoring. This work provided a reliable candidate for an effective RH monitoring system with potential application in medical diagnoses, industrial production, environmental detection, and human health monitoring.
研究领域:
光学传感
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材料科学
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生物医学工程
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环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）