SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Yanxia Xiao,Wenjuan Dong,Huiping Wang,Yumin Hao,Zihan Wang,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
A novel visual nanoprobe was developed for the sequential detection of morin and zinc ion (Zn 2+ ) based on Cl and N co-doped carbon quantum dots (ClNCQDs) via a fluorometric and colorimetric dual-readout mode. The yellow fluorescence ClNCQDs was synthesized by the one-step hydrothermal treatment of o -chlorobenzoic acid and p -phenylenediamine. The most distinctive property of the ClNCQDs is the large stokes shift (177 nm), which is significantly higher than other reported CQDs. The fluorescence of the ClNCQDs can be effectively quenched by morin based on the synergistic effect of IFE, electrostatic interaction, and dynamic quenching process, and recovered upon the addition of Zn 2+ due to strong interaction between morin and Zn 2+ . The nanoprobe exhibited favorable selectivity and sensitivity toward morin and Zn 2+ with detection limits of 0.09 µM and 0.17 µM, respectively. Simultaneously, the color of the ClNCQDs solution was changed (light-pink → faint-yellow → dark-yellow) along with the variation of the fluorescence signal of the ClNCQDs. This proposed nanoprobe was successfully applied for morin and Zn 2+ analyses in actual samples and live cells with high accuracy. The results of this study demonstrate the great application prospects of the ClNCQDs for morin and Zn 2+ detection in complex actual samples and biosystems.
研究领域:
纳米技术
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分析化学
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材料科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2021
·Vol. 188
·期 10
·1-10
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IF 5.833
Chen He,Sun Wanlin,Zhang Zhongrong,Tao Zhexuan,Qin Yuling,Ding Yuan,Wang Limin,Wang Minghua,Hua Xiude
Two high-sensitivity competitive immune-nanoplatforms based on the inner filter effect (IFE-IN) and magnetic separation (MS-IN) with a positive readout were developed to rapidly detect imidacloprid (IMI) using gold nanoparticles (AuNPs). For IFE-IN, IMI competes with AuNPs-labeled IMI antigens (IMI-BSA-AuNPs) to bind with anti-IMI monoclonal antibody (mAb)-conjugated NaYF 4 :Yb,Er upconversion nanoparticles, which changes the fluorescence signal at excitation/emission wavelength of 980/544 nm. For MS-IN, the immunocomplex of IMI-BSA-AuNPs and magnetic-nanoparticles-labeled mAb (mAb-MNPs) dissociates in the presence of IMI, and the optical density of IMI-BSA-AuNPs at 525 nm increases with the IMI concentration after magnetic separation. Under the optimal conditions, the IMI concentration producing a 50% saturation of the signal (SC 50 ) and linear range (SC 10 − SC 90 ) were found to be 4.30 ng mL −1 and 0.47 − 21.37 ng mL −1 for IFE-IN, while 1.21 ng mL −1 and 0.07 − 10.21 ng mL −1 for MS-IN, respectively. Both IFE-IN and MS-IN achieved excellent accuracy for the detection of IMI in different matrices. The quantities of IMI in apple samples detected by IFE-IN and MS-IN were consistent with the high-performance liquid chromatography results. Graphical abstract For IFE-IN, analyte competes with AuNPs-labeled-antigen to bind with the mAb-conjugated-UCNPs, which changes the fluorescence signal at 544 nm. For MS-IN, the immunocomplex of AuNPs-labeled-antigen and mAb-conjugated-MNPs dissociates in the presence of analyte, and the optical density of AuNPs-labeled-antigen at 525 nm increases with increasing analyte concentration after separation.
研究领域:
分析化学
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纳米技术
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
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IF 3.571
Chunhua Li,Li Zhu,Weixia Yang,Xie He,Sheliang Zhao,Xiaoshuo Zhang,Wenzhi Tang,Jianlong Wang,Tianli Yue,Zhonghong Li
A fluorescent method for detection of tetracyclines (TCs) in milk was developed by using the NH2-MIL-53(Al) nanosensor synthesized via a one-pot hydrothermal method. The nanosensor had a crystalline nanoplates structure with rich groups of −NH2 and −COOH. The −NH2/–COOH of NH2-MIL-53(Al) reacted with the −CO–/–OH of TCs to form a complex. The electron of −NH2/–COOH from the NH2-BDC ligand transferred to the −CO–/–OH of TCs. −NH2 of the NH2-MIL-53(Al) interacted with the −CO–/–OH of TCs by hydrogen bonding. The quenching efficiency of the inner filter effect (IFE) was calculated to contribute 57–89%. The synergistic effect of photoinduced electron transfer (PET) and IFE account for fluorescence quenching. TCs were quantitatively detected in milk samples with recoveries of 85.15–112.13%; the results were in great accordance with high-performance liquid chromatography (HPLC) (P > 0.05), confirming the NH2-MIL-53(Al) nanosensor has potential applicability for the detection of TCs in food matrix.
研究领域:
分析化学
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材料科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Lijuan Yang,Lumin Zeng,Yongqing Tao,Dandan Wang,Kailian Zhang,Meng Tian,Zhining Xia,Die Gao
The reuse of waste from agriculture or food is of great significance. Herein, an efficient and green approach for the synthesis of N-CDs with GGEC, a waste from chicken, as precursor was reported for the first time. The obtained N-CDs with outstanding blue fluorescence demonstrated high quantum yield (QY) of 19.45%. Further, the optical and physicochemical properties of N-CDs were characterized through UV–vis, FL spectroscopy, TEM, FT-IR and XPS. Interestingly, the N-CDs can be acted as a sensitive and selective sensing platform for nitroimidazoles including MNZ, TNZ, ONZ and SNZ with the fluorescent quenching mechanisms of IFE and static quenching. The LODs of them were separately determined as 0.267 μM, 0.067 μM, 0.133 μM, 0.067 μM, which were satisfying in comparison with the reported fluorescence detection method. Furthermore, the N-CDs were also successfully applied to the detection of nitroimidazoles in the real samples (e.g. tablets, chicken and plasma) with satisfactory results. Eventually, the cytotoxicity and bio-imaging applications of N-CDs were conducted and the results confirmed the potential application of it in biological field.
研究领域:
材料科学
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分析化学
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生物医学工程
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环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）