JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhencong Yang,Xue Feng,Philippe Madjirebaye,Yuting Fan,Li Yan,Zhoujin Xu,Xuli Wu
Although sodium nitrite (SN) is broadly used as a food preservative, its immunomodulatory effects on food allergy pathogenesis remain poorly understood. This study examined how subchronic SN exposure influences dendritic cell (DC)-mediated sensitization and T-cell polarization in an Ovalbumin (OVA)-induced mouse food allergy (FA) model. Our findings reveal that SN exacerbated allergic responses manifested by increased clinical symptoms, reduced weight gain, and elevated serum histamine, MCP-1, and IgE levels compared to those of OVA-sensitization alone. Mechanistically, SN promoted Th2 polarization through T-bet/GATA3 imbalance, Treg suppression, and enhanced DC activation. In vitro studies have demonstrated augmented antigen uptake by SN, MHC II expression, and selectively activated MAPK (p-JNK/ERK1/2) and STAT6 pathways, amplifying Th2 cytokine production and IgE class switching. These findings indicate that SN might disrupt immune homeostasis through coordinated modulation of DC function, Th2 polarization, and regulatory mechanisms, which highlights the potential of SN to exacerbate FA via multiple immunomodulatory pathways.
研究领域:
免疫学
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过敏与免疫学
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分子免疫学
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营养免疫学
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黏膜免疫学
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信号转导
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食品毒理学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Lidong Pang,Chen Chen,Shihang Li,Jia Shi,Yue Su,Xinyan Yang,Yujun Jiang
Whey protein isolate (WPI) is an important food ingredient, but its high allergenicity limit its application. Recently, metal–phenolic networks (MPNs) have been shown to be effective in modifying proteins. The aim of this study was to evaluate the effects of MPNs formed from (−)-epigallocatechin-3-gallate (EGCG) and Fe 3+ on the structure, antibody-binding capacity, and functional properties of WPI. The results showed that MPN modification significantly reduced the antibody-binding capacity of WPI with immunoglobulin E and IgG binding reduced by 76.40 % and 84.74 %, respectively. Multispectroscopy and liquid chromatography–tandem mass spectrometry analyses suggest that this reduction is due to the destruction of conformational epitopes and the masking of linear epitopes by MPNs. Additionally, the introduction of phenolic hydroxyl groups through MPN modification led to conformational unfolding of WPI and reduced surface hydrophobicity, thereby enhancing its antioxidant activity, hypoglycemic ability, and foaming properties. These improvements were more pronounced at higher doses. Specifically, at a dosage of 96 mM, the total antioxidant capacity is 44.01 % and the foaming properties are 104 %. This study provides new insights into the modification of WPI and offers a promising strategy for developing hypoallergenic foods products.
研究领域:
食品科学
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过敏研究
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蛋白质化学
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生物功能特性
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
·
2022
·
IF 3.887
Ding TANG,Chen WANG,Qianying GAN,Zhixin WANG,Renwang JIANG
Guominkang (GMK), a Chinese medicine formula, has been used to treat allergic diseases in clinical settings for many years. To evaluate the antiallergic effect and molecular mechanism of action of GMK extract, RBL-2H3 cell models and passive cutaneous anaphylaxis (PCA) mouse models were established. High performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analyses were performed to characterize the chemical composition of GMK. A total of 94 compounds were identified or tentatively identified from GMK. Three of them, emodin, ursolic acid, and hamaudol, were identified for the first time as potential active compounds in GMK, since they inhibited the degranulation of mast cells. The anti-allergic effect of hamaudol was the first to be discovered. GMK could markedly mitigate the shade of Evans Blue extravasation and ear incrassation in PCA mouse models. Additionally, GMK significantly inhibited the degranulation of mast cells, suppressed mast cell degranulation by reducing Ca 2+ influx and the levels of TNF- α , IL-4, and histamine, and markedly inhibited the phosphorylation of Lyn, Syk, PLC γ 1, I κ B α , and NF- κ B p65. Molecular docking results indicated that hamaudol and emodin had strong interaction with FcɛRI and NF- κ B related proteins, while ursolic acid only interacted with NF- κ B associated proteins. These results suggest GMK suppresses the activation of MCs both in vivo and in vitro . The underlying mechanism of its anti-allergic activity is associated with the inhibition of FcɛRI and NF- κ B activation.
研究领域:
药理学
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免疫学
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中医
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yongniu Zhang,Er-Fang Ren,Tao Wen,Shijun Lyu,Lili Gai,Siyu Chen,Kai Li,Zhong Han,Fuge Niu,Debao Niu
The study aimed to investigate the allergenicity change in casein treated with dielectric barrier discharge (DBD) plasma during in vitro simulated digestion, focusing on the immunoglobulin E (IgE) linear epitopes and utilizing a sensitized-cell model. Results indicated that prior treatment with DBD plasma treatment (4 min) before simulated digestion led to a 10.5% reduction in the IgE-binding capacity of casein digestion products. Moreover, the release of biologically active substances induced from KU812 cells, including β-HEX release rate, human histamine, IL-4, IL-6, and TNF-α, decreased by 2.1, 28.1, 20.6, 11.6, and 17.3%, respectively. Through a combined analysis of LC-MS/MS and immunoinformatics tools, it was revealed that DBD plasma treatment promoted the degradation of the IgE linear epitopes of casein during digestion, particularly those located in the α-helix region of α s1 -CN and α s2 -CN. These findings suggest that DBD plasma treatment prior to digestion may alleviate casein allergic reactions.
研究领域:
食品科学
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免疫学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 23
·5871-5878
·
IF 3.307
Wang Yu,Cui Min,Jiao Mingxia,Luo Xiliang
Accurate detection of protein biomarkers in complex media remains a challenge due to severe nonspecific adsorption and biofouling, and sensing interfaces that combine the high sensitivity and antifouling ability are highly desirable. Herein, an antifouling sensing interface capable of sensitively assaying immunoglobulin E (IgE) in biological samples was constructed. The sensing interface was fabricated through the self-assembly of a zwitterionic peptide and the IgE aptamer onto a macroporous Au substrate, which was electrochemically fabricated with the aid of multilayer polystyrene nanospheres self-assembled on glassy carbon electrode. Due to the huge surface area arising from porous morphology and high specificity of aptamer, the developed electrochemical biosensor exhibits ultrahigh sensitivity and selectivity towards IgE, with the linear range of 0.1–10 pg mL −1 , and a very low limit of detection down to 42 fg mL −1 . Interestingly, owing to the presence of the zwitterionic peptide, the biosensing interface can satisfyingly reduce the nonspecific adsorption and fouling effect. Consequently, the biosensor was successfully applied to detect IgE in complex biological samples, indicating great promise of this peptide-based sensing interface for antifouling assays.
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研究领域:
分析化学
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生物传感器
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电化学
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生物分析科学
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DOI
PubMed
高影响因子
American Journal of Rhinology & Allergy
·
2021
·
IF 2.467
Zhiqi Ma,Haijuan Lian,Xiaoyan Lin,Yong Li
BackgroundAllergic rhinitis (AR) is one of the most common noninfectious respiratory diseases caused by immunoglobulin E (IgE) response.ObjectiveThe study sought to explore the relationship between lncRNA MIAT and miR-10b-5p and their interaction in the regulation of allergic phenotypes in allergic rhinitis (AR) mice.MethodsA mice model of AR was constructed using ovalbumin (OVA) sensitization. AR mice were treated with miR-10b-5p agomiR and LNA mediated lncRNA MIAT. The targeting relationship between MIAT and miR-10b-5p was analyzed by the ENCORI website and dual-luciferase reporter assay. The numbers of rubbing and sneezing of mice were counted. Hematoxylin-eosin (HE) staining visualized the eosinophils infiltration in nasal mucosa tissues of mice. The percentage of Th17 cells was quantitated by flow cytometry analysis. ELISA was used to detect the levels of serum OVA-specific IgE, the Th12 cytokine IL-4, and inflammatory cytokines (IL-6, IL-17).ResultsMIAT was up-regulated in the nasal mucosa of AR mice, while miR-10b-5p was down-regulated. MIAT directly suppressed miR-10b-5p expression in AR mice. The numbers of rubbing and sneezing, the percentage of Th17 cells, and the levels of OVA-specific IgE, IL-4, IL-6, and IL-17 in AR mice were decreased by miR-10b-5p overexpression, which was reversed by MIAT overexpression. The eosinophils infiltration in AR mice was inhibited by miR-10b-5p overexpression, which was also reversed by MIAT overexpression.ConclusionThe present study demonstrates that MIAT overexpression Promotes allergic inflammation and symptoms by activating Th17 immune response via miR-10b-5p inhibition.
研究领域:
免疫学
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分子生物学
·
过敏
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xu-mei Wang,Zong-cai Tu,Yun-hua Ye,Guang-xian Liu,Yue-ming Hu,Hui Wang
This study aimed to isolate and evaluate the allergenicity of glycated α-lactalbumin (ALA) digestive products and identify its allergenic peptides. The digestive products of native-, alone glycated- and ultrasound-assisted glycated ALA (ALA-D, ALA-gal-D, 100ALA-gal-D) were isolated into three fractions (F1, F2 and F3). High-resolution mass spectrometry showed that the digestion-resistant peptides of F2 and F3 mainly distributed in amino acid sequence (AA) 25–31, AA32-53, AA40-53, AA54-60, AA80-90, AA94-104. The allergenicity of the three fractions of glycated ALA was lower than that in ALA-D, indicating glycation of ALA could indeed reduce its allergenicity after digestion. Furthermore, most fractions isolated from high glycation-degree ALA had the lowest allergenicity. The IgG/IgE binding abilities of synthesized peptides indicated that AA94-104 firstly identified by us embodied the strongest allergenicity and might be the potential allergenic peptide. This will provide a theory for preparing hypoallergenic products based on the identified allergenic peptides.
研究领域:
食品科学
·
过敏学
·
蛋白质组学
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产品关联
DOI
PubMed
高影响因子
Applied Biological Chemistry
·
2026
·Vol. 69
·期 1
·5
·
IF 2.7
Lee Serim,Kim Moonsu,Shin Seong-Ah,Moon Sun Young,Choi Seyeon,Kim Minji,Lee Jun Hyuck,Youn Ui Joung,Lee Chang Sup
An allergy is an exaggerated immune response to an otherwise harmless substance. These reactions are triggered largely by mast cell activation and the subsequent release of inflammatory mediators. Although current allergy therapies effectively relieve symptoms, they are generally accompanied by side effects, highlighting the need for safer alternatives. In this study, we investigated the anti-allergic effect of ethyl-2-oxo-2H-chromene-3-carboxylate (EtCC), a coumarin derivative. Rat basophilic leukemia (RBL-2H3) cells and a passive cutaneous anaphylaxis (PCA) model were used for in vitro and in vivo evaluations, respectively. In RBL-2H3 cells sensitized with dinitrophenyl (DNP)-specific-immunoglobulin (Ig) E and stimulated with DNP-human serum albumin (HSA), EtCC inhibited histamine and beta-hexosaminidase release, and downregulated mRNA expression of interleukin (IL)-4, IL-13, and TNF-α. EtCC also suppressed phosphorylation of MAPK, AKT, and NF-kB. In vivo, EtCC attenuated ear edema and Evans blue extravasation in a PCA model. These findings demonstrate that EtCC effectively suppresses mast-cell-mediated allergic responses, suggesting its potential as a novel allergy treatment.
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产品关联
DOI
高影响因子
ALLERGY
·
2021
·Vol. 77
·期 2
·483-498
·
IF 13.146
Renlan Wu,Xiefang Yuan,Xingjie Li,Ning Ma,Hongyu Jiang,Hongmei Tang,Guofeng Xu,Zhigang Liu,Zongde Zhang
Background Alteration of commensal microbiota is highly correlated with the prevalence of allergic reactions to food in the gastrointestinal tract. The mechanisms by which microbiota modulate food allergen sensitization in the mucosal site are not fully understood. Methods We generate DCs specific knockout of retinoic acid receptor α (Rara) gene mice (DC KO Rara) to evaluate food sensitization. The bile acid-activated retinoic acid response was evaluated by flow cytometry, real-time RT-PCR and Illumina transcriptome sequencing. The global effect of Abx treatment on BA profiles in the mucosal lymph tissue mLN in mice was examined by UPLC-MS analysis. Results In this study, we demonstrate that depletion of commensal gut bacteria leads to enhanced retinoic acid (RA) signaling in mucosal dendritic cells (DCs). RA signaling in DCs is required for the production of food allergen-specific IgE and IgG1. Antibiotics induced an enlarged bile acid (BA) pool, and dysregulated BA profiles contributed to enhanced RA signaling in mucosal DCs. BA-activated RA signaling promoted DC upregulation of interferon I signature, RA signature, OX40L, and PDL2, which may lead to T helper 2 differentiation of CD4+ T cells. BA-activated RA signaling involved the farnesoid X receptor and RA receptor α (RARa) interaction. Depletion of bile acid reduces food allergen specific IgE and IgG1 levels in mice. Conclusion Our research unveils a mechanism of food sensitization modulated by BA-RA signaling in DCs, which suggests a potential new approach for the intervention of food allergic reactions.
研究领域:
免疫学
·
微生物学
·
过敏
·
分子生物学
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DOI
PubMed
高影响因子
MEDICAL SCIENCE MONITOR
·
2020
·
IF 1.918
Wenlan Wang,Xiaoming Luo,Qin Zhang,Xujun He,Zhifang Zhang,Xinxin Wang
Background Bifidobacteria are among the probiotics used in treating intestinal diseases and are rarely used for allergic asthma treatment. The present study investigated the mechanism of B. infantis in treating allergic asthma in mice. Material/Methods A total of 40 male Balb/c mice were randomized into control, ovalbumin (OVA), montelukast (Mon), and B. infantis (B10) groups, and allergic asthma was induced in the OVA, Mon, and B10 groups. Airway reactivity was measured on day 29 by methacholine at various doses. The numbers of total cells and inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted by blood cell counter and Diff-Quik staining. Hematoxylin-eosin (HE) staining was performed to observe inflammatory cell infiltration in lung tissues. Total IgE and OVA-specific IgE in serum were measured by ELISA. Mucin 5AC expression was detected by Western blot to evaluate airway obstruction. The levels of Th1 (IFN-γ, IL-2) and Th2 (IL-4, IL-5, IL-13) cytokines in BALF and tissues were detected by ELISA and qRT-PCR, respectively. Results The mice in the OVA group had airway hyperreactivity, while the symptoms in the B10 group and Mon group were effectively relieved. B10 reduced the number of inflammatory cells in BALF as well as inflammatory cell infiltration in tissues. Moreover, the levels of total serum IgE, OVA-specific IgE, and Mucin 5AC were increased in the OVA group, but were reduced in the Mon group and B10 group. B. infantis increased the levels of Th1 cytokines and decreased those of Th2 cytokines. Conclusions B. infantis can reduce the infiltration of inflammatory cells induced by OVA-specific antibodies in mice. B. infantis has therapeutic effects on allergic asthma by promoting Th1 and inhibiting Th2 immune responses.
研究领域:
免疫学
·
微生物学
·
肺科学
·
过敏
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Lidong Pang,Qiuying Zhang,Yue Gao,Jia Shi,Ling Guo,Wei Zhang,Xinyan Yang,Yujun Jiang
s The development of the food industry has led to an increasing demand for whey protein raw materials with hypoallergenic and excellent functional properties. In this study, five polyphenols, chlorogenic acid (CHA), epigallocatechin gallate (EGCG), caffeic acid (CA), gallic acid (GA) and quercetin (QE), were selected to investigate the effect of metal phenolic networks (MPNs) on whey protein isolate (WPI) on the structure, immunoglobulin E (IgE) binding capacity and functional properties. The results show that polyphenols containing more phenolic hydroxyl groups to construct catechol MPNs (EGCG-Fe 3+ ) and multimodal MPNs (QE-Fe 3+ ) lead to a more de-folded and flexible structure of WPIs. This resulted in their modified WPIs exhibiting lower IgE binding capacity (80.08% and 86.90%) and excellent solubility, antioxidant activity, emulsification properties and foaming properties. This study provides a novel and effective potential strategy for WPI modification. Furthermore, this approach shows potential in reducing the allergenicity of WPI while improving the functional properties.
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yiling Zhou,Yaru Liao,Jing Bai,Xinlei Xia,Zhihua Wu,Xin Li,Ping Tong,Hongbing Chen,Anshu Yang
This study aimed to investigate the effects of active soybean isoflavones on the structure and potential allergenicity of glycinin, a major allergen, in soybean. The interaction mechanism between two active soybean isoflavones, namely, genistein (Gen) and daidzein (Dai), and glycinin were investigated using a series of spectral techniques. The structure of the isoflavone-glycinin complexes was characterized by SDS-PAGE and spectral techniques. In addition, the potential allergenicity of the isoflavone-glycinin complexes and their digestion products in vitro was measured via Western blot and enzyme-linked immunosorbent assay. Results showed that Gen and Dai effectively quenched the intrinsic fluorescence of glycinin largely through static quenching. Dai interacted with glycinin through hydrogen bonding and van der Waals forces, while Gen relied on hydrophobic interactions with glycinin. Furthermore, the neutral complex of daidzein-glycinin (Dai-G) exhibited weaker IgE binding ability compared with the glycinin samples. These findings provided new insights into the molecular mechanisms that underlay the immunomodulatory effects of soybean isoflavones and their potential applications in food allergy prevention and management.
CAS / MDL:
研究领域:
食品科学
·
过敏与免疫学
·
分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）