FOOD CHEMISTRY
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2024
·
IF 8.5
Zhihong Xuan,Wenjie Shen,Hongmei Liu,Baoxia Ni,Ziye Lian,Li Li,Jinnan Chen,Baoyuan Guo,Songxue Wang,Jin Ye
This study presents a novel approach toward the one-pot green synthesis of ZIF-8/IgG composite, focusing on its precise orientation and protection of the anti-aflatoxins antibody. The antibody orientation is achieved through the specific binding of IgG to the Fc region of the antibody, while the antibody protection is accomplished by the structural change restriction of ZIF-8 framework to the antibody. Consequently, the antibody exhibits enhanced target capability and significantly improved tolerance to organic solvents. The ZIF-8/IgG/anti-AFT was employed for the purification and detection of AFTs by coupling with UPLC. Under optimized conditions, the recoveries of spiked AFTs in peanut oils are between 86.1% and 106.4%, with relative standard deviations (RSDs) ranging from 0.8% to 8.8%. The linearity range is 0.5–20.0 ng for AFB 1 and AFG 1 , 0.125–5.0 ng for AFB 2 and AFG 2 , the limit of detection is 0.1 ng for AFB 1 and AFG 1 , 0.03 ng for AFB 2 and AFG 2 .
研究领域:
生物化学
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食品科学
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环境分析
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
·
IF 4.006
Xu-mei Wang,Yun-hua Ye,Zong-cai Tu,Yue-ming Hu,Hui Wang,Cheng-yan He
This study aimed to investigate the mechanism underlying the influence of mild glycation on the digestibility and anaphylactic effect of β-lactoglobulin (βLG) and its digests through LC Orbitrap MS/MS. Three glycated sites were discovered after glycation with fructose, whereas only lysine47 (K47) was glycated with maltose . After digestion, the intrinsic fluorescence intensity decreased and red shifted, and the degree of hydrolysis (DH) values showed no significant difference among digests. βLG glycated with Fru showed the lowest IgG and IgE-binding abilities. Meanwhile, the IgG and IgE-binding abilities further decreased and showed no apparent difference among digests. These results were attributed to the proximity to the IgG-binding epitope, the high glycation degree of K47, and mild glycation. Therefore, mild glycation could reduce the anaphylaxis of proteins before digestion, but did not influence the anaphylactic effect of the digests.
研究领域:
食品科学
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免疫学
·
蛋白质组学
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DOI
高影响因子
Biomolecules
·
2024
·Vol. 14
·期 12
·1568
·
IF 4.8
Rui Wang,Junping Xue,Guo Wei,Yimeng Zhang,Chuanliang Wang,Jinhua Li,Xuhui Geng,Abbas Ostovan,Lingxin Chen,Zhihua Song
The development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance forCOVID-19diagnosis and effect of vaccination evaluation. In this investigation, an SiO2@Au@CDs nanoparticle (NP)-based lateral flow immunoassay (LFIA) strip was fabricated and coupled with a miniaturized fluorimeter. The morphology features and particle sizes of the SiO2@Au@CDs NPs were characterized carefully, and the results indicated that the materials possess monodisperse, uniform, and spherical structures. Finally, this system was employed for SARS-CoV-2 IgG antibody test. In this work, the strategy for the SARS-CoV-2 IgG antibody test possesses several merits, such as speed (less than 15 min), high sensitivity (1.2 × 10−7mg/mL), broad linearity range (7.4 × 10−7~7.4 × 10−4mg/mL), accurate results, high selectivity, good stability, and low cost. Additionally, future trends in LFAs using quantum dot-based diagnostics are envisioned.
研究领域:
医学诊断
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纳米技术
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病毒学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Yong He,Yingran Wang,Yanli Shi,Zhifeng Fu
Timely and accurate anti-infective therapy significantly benefits treatment of various life-threatening bacterial infectious diseases. Unfortunately, owing to the lack of selectivity for recognizing bacterial species, most of the reported phenotypic antimicrobial susceptibility testing protocols suffer from time-consuming bacterial isolation and identification. In this work, a highly-selective molecular recognition-based sandwich fluorimetric assay was developed to rapidly detect Staphylococcus aureus ( S. aureus ), and furtherly to assess its susceptibility to antibiotics. Immobilized porcine IgG was utilized to capture S. aureus through the selective interaction between Fc fragment of porcine IgG and protein A in the cell wall of S. aureus . Fluorescein isothiocyanate-labeled teicoplanin was adopted as the signal tracer utilizing the binding behavior between teicoplanin and d -Ala- d -Ala moieties in the peptidoglycans of Gram-positive bacterial cell wall. S. aureus could be detected within a wide linear rang of 1.0 × 10 3 to 1.0 × 10 7 CFU mL −1 . Subsequently, this protocol utilizing porcine IgG and teicoplanin as the indicator molecules was utilized to assess the antimicrobial susceptibility of S. aureus after the bacteria were treated with antibiotics. The minimum inhibitory concentrations of penicillin, cefoxitin, clindamycin, trimethoprim/sulfamethoxazole and erythromycin were estimated to be >0.25,<4, <0.25, <2/38 and <0.5 μg mL −1 , respectively. The whole process for antimicrobial susceptibility testing can be completed within 4 h since it avoided extra time-consuming isolation and identification of bacterial species.
研究领域:
微生物学
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医学诊断
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药学
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DOI
高影响因子
BIOELECTROCHEMISTRY
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2022
·
IF 5.76
Zixuan Liu,Zheng-Zhi Yin,Guojun Zheng,Hongyu Zhang,Min Zhou,Shan Li,Yong Kong
Detection of immunoglobulins (Igs) is of clinical significance for early diagnosis and timely treatment of diseases. Herein, a dual-template molecularly imprinted (DTMI) electrochemical biosensor was developed for IgG-IgM combined assay. In this DTMI electrochemical biosensor, Prussian blue (PB) and thionine (TH) decorated on graphene oxide (GO) and multi-walled carbon nanotubes (MWCNTs), respectively, were utilized as the dual-signal probes, and Au nanoparticles (AuNPs) were used for Igs anchoring and signal amplification. Polypyrrole (PPy) was electrodeposited on the biosensor surface and acted as the molecularly imprinted polymers (MIPs). After the removal of the IgG and IgM templates, the resultant DTMI electrochemical biosensor was used for IgG-IgM combined assay, and the concentrations of IgG and IgM could be indicated by the changes in the peak currents of PB (Δ I PB ) and TH (Δ I TH ), respectively. The DTMI electrochemical biosensor displayed a wide linear range and a low limit of detection (LOD) for both IgG (28.80 pg mL −1 ) and IgM (0.58 pg mL −1 ). Finally, the developed DTMI biosensor was used for IgG-IgM combined assay in clinical serum samples, and the results were comparable to those obtained by conventional immunoturbidimetry, implying its great potential in clinical diagnosis.
研究领域:
分析化学
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生物传感器
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电化学
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纳米技术
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2021
·Vol. 79
·期 8
·6409-6429
·
IF 2.87
Ding Guanjun,Wang Ao,Shi Xinming,Li Jumei,You Lijun,Wang Shaoyun
Polyphosphazene microspheres (PZS) multispectral encoded by fluorescence and surface enhanced Raman spectra (SERS) were prepared and used as solid phase carrier of capture antibody for antibody detection. A variety of fluorescent encoded polyphosphazene microspheres (FPZS) incorporating single and multiple fluorescent molecules were prepared. Multiple SERS encoded polyphosphazene microspheres (SPZS) were obtained by modification of different Raman reporters on the surface of PZS@Ag, which were fabricated with rough morphology through in situ deposition of Ag nanoparticles. Fluorescence and SERS spectral combined encoded polyphosphazene microspheres (FSPZS) were prepared by encoding of fluorescent molecules and SERS reporters. The optical signals in the two independent detection channels were separable and ensured the accuracy for the encoding method. Immunoassays of goat IgG, rabbit IgG and mouse IgG were performed by the as-prepared FPZS, SPZS and FSPZS as solid phase carrier of capture antibody and fluorescent labeled second antibody, using sandwich immunoassay method. The immunoassay results showed that the multiple-spectra encoded microspheres have stable and distinguishable encoding signals, large encoding volume and multiplexed analytical capability in sandwich immunoassays.
研究领域:
材料科学
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分析化学
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生物传感器
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免疫学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
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2018
·
IF 4.439
Wenfei Zhou,Mo Yang,Zhi Zhao,Shaopeng Li,Zhiqiang Cheng,Jinsong Zhu
Surface-grafted polymer brushes have been long reported as a desired matrix for biosensing applications, owing to enhanced immobilization capacity and anti-fouling properties. However, simultaneous optimizing binding signal and minimizing non-specific adsorption using polymer matrix is still a challenge, due to the lack of systematic structural and composition optimization at molecular level. In this research, a series of block copolymer brushes with controlled hierarchical architectures, poly [oligo (ethylene glycol) methacrylate- b -glycidyl methacrylate] (POEMGA- b -PGMA) were prepared by two-stage surface-initiated polymerization. Surface plasmon resonance imaging (SPRi), as a real-time and label-free biosensing technology, was used as means to evaluate their detecting performances of bio-interactions. In contrast to one-layer poly (glycidyl methacrylate) (PGMA), 4.7-fold enhancement of signal-to-background ( S / B ) was achieved in bi-layer POEGMA- b -PGMA surface, by using rabbit IgG (R-IgG, in 10% serum) and a microarray of goat-anti-rabbit IgG (G-R-IgG) as the system of study. Interestingly, the binding signals and anti-fouling performances of the polymer brushes could be enhanced simultaneously by increasing the length of the monomer in the primary layer. Theoretically, it mainly owed to the formation of a densely packing protective primary layer against non-specific physical adsorption and a spatially optimized secondary layer of minimized steric hindrance that allowed optimum binding of analytes.
研究领域:
材料科学
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生物传感器
·
高分子化学
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2011
·
IF 5.361
Cuifang Li,Zhifeng Fu,Zongyun Li,Zhenxing Wang,Wei Wei
A disposable electrochemiluminescent (ECL) immunosensor array was fabricated on a screen-printed carbon electrode (SPCE) substrate to perform multiplexed immunoassay (MIA) for the first time. The SPCE substrate was composed of an array of four carbon working electrodes, one common Ag/AgCl reference electrode, and one common carbon counter electrode. The immunosensor array was constructed by site-selectively immobilizing multiple antigens on different working electrodes of the SPCE substrate. With a competitive immunoassay format, the immobilized antigens competed with antigens in the sample to capture their corresponding tri(2,2′-bipyridyl)ruthenium(II)-labeled antibodies. The ECL signals from the immunosensors in this array were sequentially detected by a photomultiplier with the aid of a homemade single-pore-four-throw switch. Due to the ECL readout mechanism and the sequential detection mode, it could avoid the cross-talk between the adjacent immunosensors, which was common in other reported immunosensor array. Human, rabbit and mouse immunoglobulin Gs were near-simultaneously assayed as the model analytes. The linear ranges for them were 10–400, 20–400, and 20–400 ng/mL, with detection limits of 2.9, 6.1 and 6.5 ng/mL (S/N = 3), respectively. This novel ECL strategy based on immunosensor array coupled with non-array detector provided a simple, sensitive, low-cost and time-saving approach for MIA. It showed great application potential in point-of-care test and field analysis of bio-agents, with mass production potential and high throughput.
研究领域:
分析化学
·
生物化学
·
生物医学工程
·
即时检测
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Lijun You,Baohua Zhong,Ci Huang,Jumei Li,Zhijuan Zheng,Yang Wang
Au coated magnetic polyphosphazene (MPCTP) composite particles ( [email protected] ) were fabricated with sensitive SERS activity. The MPCTP particles were generated by coating polyphosphazene on Fe 3 O 4 nanoparticles through precipitation polycondensation of hexachlorocyclotriphosphazene and phloroglucinol . [email protected] composite particles were obtained by deposition of Au nanoparticles on MPCTP by the reduction of HAuCl 4 . The size and the thickness of the Au shell can be controlled by varying the amount of HAuCl 4 . The magnetic core endowed the composite particles with good magnetic responsiveness, which allowed the analyte to be enriched and separated from the complex matrix, and significantly simplifying the sample pretreatment procedure. The SERS activity of [email protected] composite particles were evaluated by DTNB as model Raman reporter, and the limits of detection (LOD) of DTNB was 10 -8 mol/L. A high efficient SERS immunoassay system based on the [email protected] substrates for the detection of immunoproteins was developed. Human IgG and rabbit IgG were quantitatively determinated simultaneously by this immunoassay system. The quantitative determination of the immunoglobulin G (IgG) was achieved and the LOD of human IgG, rabbit IgG and the mixture of human IgG and rabbit IgG were as low as 10 fg/mL, 100 pg/mL and 1 ng/mL, respectively. The results showed that the [email protected] composite particles have broad application prospects as high performance SERS active substrates for immunoprotein analysis.
研究领域:
材料科学
·
分析化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Cuixia Guo,Ziling Ye,Yonghong He,Zeyu Huang,Hongjian Zhang,Feng Huang
In response to the urgent requirement for high-throughput multiplexed detection, a novel dual-encoded suspension array (SA) based on thickness and digital fluorescent peak is proposed, along with the corresponding optical system. The SAs are prepared by thickness encoded micro-glass chips (MGCs) with electrostatically adsorbed quantum dots (QDs). Using the thickness characteristics of microcarrier itself and digital fluorescence peak (DFP) as the encoded signals ensure the high encoding stability with simple fabrication process at a low cost. A spectral-domain phase and fluorescence spectroscopy microscopy (SDPFSM) system is designed for the first time to decode the thickness information and DFP with ultrahigh accuracy. The high selectivity and sensitivity of the dual-encoded SAs are verified by the multiplexed immunoassays . The quantitative detection of anti-human IgG, anti-mouse IgG, and anti-rabbit IgG was realized with the limits of detection (LOD) of 0.19, 0.35 and 0.31 pM/mL and the sensitivity of 713, 470 and 456 a.u./(pM/mL), respectively. The coding number increases by a factor of 458 compared with the traditional fluorescence coding method due to the additional use of the thickness information of the MGCs itself as a coding channel. The aforementioned properties demonstrate that the dual-encoded SA and the corresponding decoding platform are promising and robust in multiplexed detection.
研究领域:
生物传感器
·
分析化学
·
生物医学工程
·
光学工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Pan Cheng,Chuang Zhang,Xiupu Lu,Daiqing Peng,Pengju Zhao,Tao Mei,Shunxing Zhang,Qihao Guo,Ke Liu,Dong Wang
Immunoglobulin G (IgG) purification remains costly, largely due to limitations of conventional bead-based affinity media. This study presents a novel Protein A-functionalized nanofibrous sponge (NFS-Protein A) with hierarchically ordered lamellar pore channel structure designed for efficient IgG separation. This medium is fabricated through an integrated approach combining in-situ grafting of EVOH nanofibers with a polyethyleneimine (PEI) molecular amplifier and directional freezing technology. A key innovation is the use of PEI to exponentially amplify surface aldehyde groups, overcoming the intrinsically low grafting efficiency of the nanofiber substrate and enabling high-density, stable Protein A immobilization. The resulting sponge features long-range ordered lamellar pores (~20 μm), a high specific surface area (25.318 m²/g), and robust mechanical integrity. NFS-Protein A demonstrated a high static IgG adsorption capacity of 212.6 ± 6.3 mg/g, which is superior to reported state-of-the-art. Critically, it overcomes the throughput limitations of conventional columns by achieving a hydraulic flux of up to 30.45 ± 0.52 L/(h·bar) and a high dynamic binding capacity of 127.6 ± 4.5 mg/g at 1 mm/min, successfully capturing IgG from a protein mixture with high purity. This scalable, tunable platform integrates convective flow with specific affinity interactions, offering a transformative solution for high-throughput antibody purification.
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产品关联
DOI
高影响因子
COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Tiange Chen,Pan Cheng,Mingyue Li,Yuxi Wang,Peiling Tang,Yinuo Zhang,Qihao Guo,Qin Cheng,Tao Mei,Ke Liu,Dong Wang
Anion-exchange chromatography (AEC) is recognized as a highly effective approach for the purification of immunoglobulin G (IgG). This study introduces an innovative strategy that employs waste cellulose filter paper in the production of AEC media. A quaternized cellulose fiber membrane (CFM-QCS) was successfully fabricated that cellulose fibers were as a structural framework, glutaraldehyde (GA) as a crosslinking agent, and quaternary chitosan (QCS) as a modifying agent. Morphological and chemical characterization revealed that GA and QCS were uniformly crosslinked on the surface of the cellulose fibers, resulting in excellent mechanical properties in both dry and wet states. Benefiting from its 3D network scaffold structure, CFM-QCS demonstrated a high adsorption capacity for bovine serum albumin (BSA), with static and dynamic adsorption capacities of 605.15 mg/g and 88.63 mg/ml, respectively. After treated with extreme conditions and 10 cyclic adsorption and elution, the adsorption capacity of CFM-QCS remains almost unchanged, highlighting its excellent stability. Additionally, a CFM-QCS packed chromatography column exhibited high flux of 10.38 L/h at 0.1 MPa, which can efficiently separate IgG from a mixed solution in the presence of BSA and IgG by gravity-driven. This work presents a straightforward approach for preparing high-performance ion-exchange chromatography membranes for IgG separation.
研究领域:
生物工程
·
分离科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）