JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Hao Li,Yuwei Zhao,Yuting Kui,Yiting Wang,Ruiying Zhang,Xiaosi Sang,Jingli Shen,Qiue Cao
Herein, a novel molecularly imprinted photoelectrochemical (MIP-PEC) sensor was fabricated through integrating the ultrathin nanosheets of Fe 2 O 3 (Fe 2 O 3 NSs) with molecular imprinting technology for the ultrasensitive detection of imidacloprid (IMD). Impressively, the two-dimensional Fe 2 O 3 NSs was synthesized on an indium‑tin oxide (ITO) substrate by electrodeposition. Benefiting from its narrow bandgap and large specific surface area, the photogenerated charge separation efficiency of Fe 2 O 3 NSs (∼ 600 μA) was remarkably enhanced compared to that of the commercial Fe 2 O 3 nanoparticles (∼150 μA), which significantly improved the sensitivity of the developed MIP-PEC sensor. Furthermore, the electropolymerization technique was used to form a molecularly imprinted polymer film of IMD on the surface of Fe 2 O 3 NSs/ITO, substantially increased the detection selectivity of the proposed sensor. As a result, the designed MIP-PEC sensor successfully achieved ultrasensitive and specific as well as reliable detection of IMD with a broad linear response (5 nM ∼ 120 μM), low detection limit (1.02 nM) and long storage time (the signal only decreased by 5.03 % after 4 weeks of storage). The recoveries and relative standard deviation (RSD) for the determination of IMD in fruits and environmental water samples by the obtained MIP-PEC were in the range of 92.0 % ∼ 108.0 % and 3.2 % ∼ 4.9 % respectively, validating its field-applicability. Obviously, this work established a novel paradigm for synergistic enhancement of the analysis performance of PEC through integration of the advanced photosensitive materials with molecular imprinting technology.
研究领域:
分析化学
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环境监测
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食品安全
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生物传感器
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材料科学
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农药残留检测
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分子识别
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2026
·
IF 4.8
Chen Chen,Peng Shi,Haihua Wu,Tingting Zhang,Min Zhang,Xueyao Zhang
As key members of xenobiotic transcription factors (XFTs), CncC and Maf mediate insecticide sensitivity by regulating detoxification enzyme genes. In this study, with Locusta migratoria as a model, investigations revealed that LmCncC showed particularly high expression in the foregut, midgut, hindgut, and fat bodies. In contrast, LmMaf expression was predominantly localized to the midgut and fat bodies. Functional analysis demonstrated that RNAi-mediated knockdown of LmCncC / LmMaf significantly increased sensitivity to deltamethrin and imidacloprid. To identify the LmCncC - and LmMaf -regulated genes, a comparative transcriptomics analysis was constructed and further identified three key uridine diphosphate-glucuronosyltransferase (UGT) genes downstream of the CncC/Maf pathway. To investigate the role of UGTs in insecticide detoxification, treatment of L. migratoria with sulfinpyrazone, a UGT inhibitor, reduced UGT activity by 42.2% and enhanced sensitivity to imidacloprid and deltamethrin. Among these UGT genes, RNAi-mediated knockdown of LmUGT392C1 significantly enhanced susceptibility to imidacloprid in insecticide bioassays. CRISPR-Cas9-generated LmCncC and LmUGT392C1 mutants validated the essential role of this pathway in imidacloprid detoxification. To clarify the regulatory mechanism of the CncC/Maf signaling pathway on LmUGT392C1, a 2623 bp promoter sequence of LmUGT392C1 was obtained. Dual-luciferase reporter gene assays confirmed that co-overexpression of LmCncC and LmMaf directly activates the promoter of LmUGT392C1 . Our study elucidates the CncC/Maf- LmUGT392C1 regulatory axis in insecticide sensitivity, which is applicable to designing new formulations to overcome detoxification or identify potential new resistance mechanisms.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 26
·15762-15768
·
IF 3.108
Zideng Gao,Long Pang,Haojie Feng,Shunyi Wang,Qiuyun Wang,Mengyao Wang,Yining Xia,Shuwen Hu
Encapsulation of pesticides with polymeric materials is a promising approach to improve the efficacy of pesticide application and reduce the potential risks of pesticides to the environment and humans. In this study, a novel imidacloprid microcapsule was prepared by depositing a polydopamine (PDA) layer on imidacloprid via oxidative self-polymerization followed by a polyurea (PU) layer on PDA via copolymerization. No organic solvent or surfactant was used in the preparation process. The entrapment rate of imidacloprid was determined to be 47.28 ± 0.87%, and the pesticide loading of microcapsule was 68.36 ± 1.13%. Several characterization assays, such as Fourier infrared transform analysis, thermogravimetric analysis and contact angle analysis, were conducted to confirm the encapsulation of imidacloprid. Dynamic light scattering showed that the microcapsule size ranged from hundreds of nm to several μm, which was similar to the results obtained by scanning electron microscopy. The microcapsule had a stable dispersion up to one month in the aqueous solution. Moreover, the microcapsule was able to release imidacloprid in a sustainable manner, as the release was much slower than the non-encapsulated imidacloprid.
研究领域:
农业化学
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材料科学
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环境科学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Weilong Cheng,Yanbo Wang,Yixuan Li,Guoping Zhao
Imidacloprid, a neonicotinoid pesticide that was reported to induce intestinal damage at low doses, necessitating effective intervention strategies. As a common component in food, glycine (GLY) has the capacity to alleviate intestinal damage. However, whether it can relieve the damage caused by imidacloprid leave to be clarified. This study investigated the protective effects of GLY (2% and 5%) against imidacloprid induced (0.06 mg/kg bw/day) intestinal injury in male Wistar rats. GLY supplementation attenuated intestinal permeability and enhanced the expression of tight junction proteins. Further, the pregnane X receptor-myosin light chain 2 signaling pathway that mediating imidacloprid-induced intestinal toxicity was also found repaired. GLY intervention increased the enzyme activities of cytochrome P450 3A4 and glutathione-S-transferase, which were responding for phase I and phase Ⅱ imidacloprid metabolism. Meanwhile, reduced levels of imidacloprid and its phase I metabolites, as well as increased phase Ⅱ detoxifying glutathione conjugates, both suggesting the protective role of GLY intervention through enhancing imidacloprid detoxification metabolism. Importantly, the amino acid signaling pathway related fecal metabolic profiles and microbiota composition in imidacloprid treated rats were also modulated and improved by GLY. Totally, our findings demonstrated the protective effect of GLY on intestinal damage caused by imidacloprid exposure, which provided ideas for preventing and controlling the toxicity of food contaminants.
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PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2022
·
IF 10.508
Jun Zhang,Fei Xue,Zhonggang Wang
Aggregation-induced emission (AIE)-active aromatic tetraaldehydes are utilized as starting monomers to create two fluorescent polymers (PAN-TPEs) with hierarchical pores ranging from micropores (1.4–1.9 nm) to mesopores (3.8–26.2 nm). Their chemsensing properties for five pesticides, including imidacloprid, acetamiprid, triflumizole, lambda-cyhalothrin, and indoxacarb, are studied. It is found that the Stern–Volmer quenching coefficient and the limit of detection for imidacloprid in the water medium reach 53 745 M–1 and 28 ppb, respectively, which are much superior to those of the reported porous materials in the literature and exceed 3–10 times of the other four pesticides. Moreover, the polymers exhibit fast fluorescence response for imidacloprid (less than 4 s), and the detection sensitivity is nearly not affected by the common cations and anions. Additionally, the studies reveal that, for the polymer sample with narrower pore channels, the recognition ability for imidacloprid strongly depends on the molecular volumes of pesticides, whereas in the case of the polymer with larger pore sizes, the inner filter effect and the photoinduced electron transfer between the pesticides and the chromophore groups in the polymer skeleton become the major factors. The results are of significance for understanding the effects of porosity parameters and AIE groups in fluorescent porous polymers on the recognizable detection of imidacloprid from other pesticides.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xiaoyan Tang,Manjia Chen,Muzi Li,Huanping Liu,Hao Tang,Yang Yang
Micro(nano)plastics are ubiquitous in the environment. Among the microplastics, imidacloprid (IMI) concentration has been increasing in some intensive agricultural regions, thus receiving increased attention. However, only a few studies have investigated the interaction of nanoplastics (polystyrene (PS)) and IMI in vegetable crops. We studied the effects of positively (PS-NH 2 ) and negatively (PS-COOH) charged nanoplastics on the uptake, translocation, and degradation of IMI in Chinese flowering cabbage grown in Hoagland solution for 28 days. PS-NH 2 co-exposure with IMI inhibited plant growth, resulting in decreased plant weight, height, and root length. Translocation of IMI from the roots to the shoots was significantly lower in the presence of PS-NH 2 , whereas PS-COOH accelerated the accumulation and translocation of IMI in plants, thus potentially affecting IMI metabolism in plants. Notably, IMI-NTG and 5-OH-IMI were the two dominant metabolites. PS-NH 2 co-exposure with IMI induced significant oxidation stress and considerably affected the activities of superoxide dismutase (SOD) and peroxidase (POD), indicating that the antioxidant defense system was the main mechanism for reducing oxidative damage. Notably, both positively and negatively charged nanoplastics can accumulate in Chinese flowering cabbage. Plants in the PS-COOH alone treatment group had the highest concentration of nanoplastics in both roots and shoots. The accumulation of nanoplastics, IMI, and its metabolites in plants raises concerns about their combined potential toxicity because it compromises food safety.
研究领域:
环境科学
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农业
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植物科学
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毒理学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chunhao Cao,Wenchuan Guo
Consumption of leafy vegetables with excessive imidacloprid (IMI) can cause serious harm to the human body. To achieve rapid IMI detection, a carbon dots (CDs)-based fluorescent (FL) probe was hydrothermally prepared using O-phenylenediamine as the precursor. The morphology, particle size distribution, crystal structure, optics and chemical bond state of the as-prepared CDs were characterized. The mechanism of the CDs in detecting IMI was investigated by Fourier transform infrared spectroscopy, and the CDs’ selectivity, stability, sensitivity, and actual sample recovery were tested. The CDs showed good selectivity, stability, and anti-interference ability. Under optimum conditions, there was a strong linear relationship between the FL intensity of the CDs and the IMI concentration in the range of 0.037–0.2 mg/L. The detection limit was 0.00187 mg/kg. The CDs were successfully applied to detect IMI in lettuce, cole, spinach, and pakchoi with spiked recoveries between 81.026% and 106.803% and a relative standard deviation between 0.001 and 0.027%.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Liwen Hu,Yiou Zhang,Qi Luo,Ting Li,Weihua Lai
Lateral flow immunoassay (LFIA) offers advantages for rapid, on-site detection of pesticides. Herein, aggregation-induced emission fluorescent microspheres were modified using maleimide groups to enable site-specific coupling of recombinant protein A (rPA). Subsequently, the Fc region of an anti-imidacloprid monoclonal antibody was bound to rPA to create an oriented immunoprobe. LFIA with the oriented immunoprobe was developed for detecting imidacloprid, a widely misused neonicotinoid pesticide. The Fab orientation ratio of the oriented immunoprobe reached 91.1%, when compared with 58.1% for the non-oriented immunoprobe. The oriented immunoprobe with a higher orientation improved immunobinding kinetics, enhanced the LFIA signal, and increased assay sensitivity. The limit of detection was 0.12 ng/mL, which was lower than 0.31 ng/mL that was achieved with the non-oriented immunoprobe. In addition, LFIA with the oriented immunoprobe required 40% less antibody and 25% less coating antigen. The recovery results were consistent with HPLC–MS/MS, demonstrating accurate detection of imidacloprid in cabbage, chili, and orange. The LFIA with oriented immunoprobe proved to be a rapid and sensitive method for food-safety monitoring.
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高影响因子
Foods
·
2025
·Vol. 14
·期 19
·3423
·
IF 5.1
Chi Zhang,Xin Liu,Tailong Cai,Jianfeng Ping,Yibin Ying
Accurate and rapid extraction of pesticide residues in fruits is crucial for timely food safety monitoring. However, conventional extraction methods remain labor-intensive and time-consuming, often requiring hours for sample preparation. Here, we present a porous hydrogel microneedle (HMN) patch integrated with an automated insertion applicator as a highly efficient platform for the rapid extraction of peach juice for imidacloprid residue detection. The HMN patch, composed of polymethyl vinyl ether/maleic anhydride (PMVE/MA) polymer, was fabricated with high porosity by adjusting the porogen content. Under optimized porogen content of 3% NaHCO 3 , the developed HMN patch exhibited ultrahigh extraction efficiency, achieving a 40-fold water absorption capacity and extracting 0.6% ( w / w ) peach solids of its weight within 5 min. The extracted juice could be readily recovered through a simple elution process and was directly compatible with both high-performance liquid chromatography (HPLC) analysis and lateral flow assays. Compared with conventional destructive methods, the HMN platform offers a scalable, high-efficiency, and user-friendly solution for high-throughput pesticide extraction. The integration of the automated applicator further enhances consistency, minimizes user variability, and facilitates on-site monitoring of pesticide residues, providing a practical pathway for field-deployable food safety monitoring.
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高影响因子
ANALYST
·
2018
·Vol. 143
·期 9
·2150-2156
·
IF 3.864
Xueyan Li,Xianwen Kan
In this study, a ratiometric strategy-based electrochemical sensor was developed by electropolymerization of thionine (THI) and β-cyclodextrin (β-CD) composite films on a glassy carbon electrode surface for imidacloprid (IMI) detection. THI played the role of an inner reference element to provide a built-in correction. In addition, the modified β-CD showed good selective enrichment for IMI to improve the sensitivity and anti-interference ability of the sensor. The current ratio between IMI and THI was calculated as the detected signal for IMI sensing. Compared with common single-signal sensing, the proposed ratiometric strategy showed a higher linear range and a lower limit of detection of 4.0 × 10−8–1.0 × 10−5 mol L−1 and 1.7 × 10−8 mol L−1, respectively, for IMI detection. On the other hand, the ratiometric strategy endowed the sensor with good accuracy, reproducibility, and stability. The sensor was also used for IMI determination in real samples with satisfactory results. The simple, effective, and reliable way reported in this study can be further used to prepare ratiometric strategy-based electrochemical sensors for the selective and sensitive detection of other compounds with good accuracy and stability.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122785
·
IF 7.5
Chen Li,Yunfeng Yang,Zhenzhen Wang,Jianfei Wang,Xinyu Song,Xiangfen Cui,Haoran Song,Qun Zhao,Shugen Liu,Senlin Tian
The widespread and persistent presence of imidacloprid (IMD), a highly toxic neonicotinoid pesticide, in aquatic environments poses significant and growing threats to ecosystems and human health. Conventional electrochemical advanced oxidation processes often rely on soluble supporting electrolytes, which introduce secondary pollution and increase operational costs. To overcome this limitation, we introduce a novel electrolyte-free solid polymer electrolyte (EP-SPE) system built upon a Nafion® N324 membrane for the efficient and sustainable electrochemical degradation of IMD. Crucially, this system eliminates the need for external supporting electrolytes, marking a significant advancement towards simplified and greener treatment technologies. The SPE system achieved 74.12% IMD removal within 240 min, which corresponded to a pseudo-first-order reaction rate constant of k = 5.72 × 10 −3 min −1 . The specific energy consumption was quantified at approximately 30.39 kW∙h/mmol IMD, which was only about one-twentieth of a conventional Na 2 SO 4 -assisted system. This performance enhancement is attributed to a membrane-induced thin-layer effect that improves mass transfer, reduces ohmic losses, and increases current density, thereby facilitating efficient ∙OH generation and pollutant interaction. Degradation pathway analysis revealed multiple transformation routes, with most intermediates being less toxic than IMD. The system showed broad pH adaptability and maintained high efficiency in real water matrices. Stable performance over multiple cycles underscores the potential of the SPE system as a sustainable and efficient alternative for electrochemical treatment of refractory organic wastewater.
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高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 9
·2048-2052
·
IF 3.223
Lei Liu,Jiawei Guo,Longhua Ding
An electrochemical sensor based on raffia derived porous carbon (RPC) and polyaniline (PANI) composite functional glass carbon electrode (GCE) was constructed for imidacloprid (IMI) determination. PANI nanowire arrays were deposited on RPC surface uniformly without aggregations. The electrochemical response of IMI at RPC@PANI/GCE is about four times than that at bare GCE, indicating high electrocatalytic activity of RPC@PANI towards IMI reduction. The prepared sensor also offers a wide linear range of 0.1–70 μg mL −1 for IMI determination with a limit of detection (LOD) of 0.03 μg mL −1 . In addition, it offers high recoveries with testing real samples.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）