TALANTA
·
2025
·
IF 6.1
Ruru Chai,Jing Zeng,Alai Hailaxi,Yueling Song,Zhengchong Shen,Guixin Li
This study presents a highly sensitive cysteine (Cys) sensing platform based on a solvent-responsive aggregation-induced electrochemiluminescence (AIECL) probe. By modulating the DMF/PBS solvent ratio, the aggregation state of tetraphenylethylene derivative (TPE-1-CHO) was optimized, resulting in a significant enhancement of its cathodic electrochemiluminescence (ECL) performance. Experimental results revealed that at an aqueous fraction ( fw ) of 50%, the aggregation degree of TPE-1-CHO and its ECL efficiency reached an optimal balance, yielding an ECL intensity substantially higher than that in pure DMF ( fw = 0%). Based on this phenomenon, an AIECL sensing platform was constructed using K 2 S 2 O 8 as a co-reactant, and the proposed platform can effectively discriminate structurally similar biomolecules based on their reducing functional group. Given its strong ECL suppression, Cys was chosen as the target analyte to assess the analytical characteristics of the presented AIECL sensing strategy. The developed sensor exhibits exceptional sensitivity, outstanding stability, and favorable selectivity. The method was effectively utilized for the quantitative detection of Cys in human serum samples. This study offers an innovative approach for the detection of reducing bioactive small molecules.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yunfei Wang,Wenjie Sun,Yixiao Li,Xuming Zhuang,Chunyuan Tian,Feng Luan,Xiuli Fu
Human immunodeficiency virus (HIV) is still a significant global health concern. Hence, developing a rapid and sensitive method for the detection of HIV is important in early detection. In this paper, we focus on the preparation of a sensitive electrochemical luminescence (ECL) biosensor that uses Ti 3 C 2 T x modified ZIF-8 as an ECL emitter to detect HIV-1 protein. This ECL biosensor used 0.05 M K 2 S 2 O 8 as the co-reactant and conductive carbon black combined with magnetic nanoparticles as the quenching agent. Under the optimized experimental conditions, the relationship was linear in the range from 1 fM to 1 nM (R 2 = 0.993) and the detection limit was 0.3 fM (S/N = 3). Finally, we used the constructed Ti 3 C 2 T x /ZIF-8 nanocomposites to detect HIV-1 in real serum samples and obtained great recoveries. The results demonstrated that this biosensor, which not only exhibited improved electrical conductivity but also demonstrated an excellent ECL response, could be a promising candidate for HIV-1 detection.
研究领域:
生物传感器
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分析化学
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材料科学
·
病毒学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Mengsi Li,Caixia Wang,Liming Chen,Defang Liu
Electrochemiluminescence resonance energy transfer (ECL-RET) between quantum dots (QDs) was firstly proposed. In this work, boron nitride quantum dots (BNQDs) as the donor and nitrogen doped graphene quantum dots (NGQDs) as the acceptor were confirmed by the absorption spectrum , the emission spectrum and fluorescence spectrum . Based on the reaction between FA and the SO 4 •− in the ECL system of NGQDs/BNQDs/K 2 S 2 O 8 , the ECL sensing platform for FA was successfully constructed. Surprisingly, a stable and strong ECL signal was obtained based on the RET, which was used for signal-off detection of FA in the presence of coreactant K 2 S 2 O 8 . Notably, about 10-fold enhancement was observed compared with the absence of BNQDs. The proposed sensor showed wide linear ranges of 1.0 × 10 −11 M to 1.0 × 10 −4 M and a low detection limit of 5.13 × 10 −12 M. Simultaneously, the sensor was successfully applied to detection of FA in human serum samples with excellent recoveries. Therefore, the NGQDs/BNQDs system provided a new perspective for development of novel ECL-RET sensors.
研究领域:
分析化学
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材料科学
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生物化学
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纳米技术
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高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2019
·
IF 1.479
Yun Ying Zhai,Yan Bin Yun,Chun Li Li
Through precipitation polymerization approach, bovine serum albumin (BSA) molecularly imprinted polymer (MIP) was synthesized with various reagents to ascertain the optimal synthesis condition. The optimized synthesis condition is methacrylic acid (MAA) as functional monomer, N,N′-methylenebisacrylamide (MBA) as crosslinker, template molecular/functional monomer/initiator ratio of 1:6:3, and K2S2O8/NaHSO3 initiator ratio of 1:2. BSA adsorption by synthesized MIP conformed to second-order kinetic adsorption equation and Langmuir adsorption isotherm. The maximum adsorption amount of MIP was 106.2 mg/g. Moreover, when the lysozyme (LYZ) was introduced as interfering adsorbate, the separation factor was up to 3.09, indicating that the MIP has a high affinity towards BSA.
研究领域:
高分子科学
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分析化学
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材料科学
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高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 17
·1739-1746
·
IF 3.532
Zixuan Chen,Yinan Li,Haixin Qin,Xiaoyan Yang,Wei Cao
β-Amyloid (Aβ) peptides can bind both Cu2+ and heme cofactors simultaneously to form heme–Cu2+–Aβ complexes, which are proposed to generate toxic partially reduced oxygen species (PROS, e.g., H2O2) and play a vital role in Alzheimer's disease (AD). In this paper, a competitive dual-mechanism-driven electrochemiluminescence (ECL) aptasensor integrating the synergistic enhancement and steric hindrance effect was described for Aβ detection. Specifically, graphite carbon nitride (g-C3N4) as an effective ECL luminescent substrate and Au nanoparticles were sequentially assembled on the Au electrode surface, and then a thiol-modified aptamer for capturing Aβ peptide was attached to the surface of the electrode through the Au–S bond. Aβ peptides were simultaneously incubated with heme and Cu2+, and the forming heme–Cu2+–Aβ complexes were subsequently anchored on the electrode through the specific recognition between the target Aβ and the aptamer. When the concentration of the target Aβ is low, the synergistic enhancement effect arising from K2S2O8 with in situ generated H2O2 is predominant, resulting in an increase in the ECL signal of g-C3N4. In contrast, when the concentration of Aβ is high, the steric hindrance effect generated from heme–Cu2+–Aβ complexes is dominant, leading to a decrease in the ECL signal. The present sensor exhibits a favorable linear response for the detection of Aβ with a relatively low detection limit of 0.24 pM, and provides a more sensitive and selective platform for bioanalysis.
研究领域:
生物化学
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分析化学
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神经科学
·
诊断学
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DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 1
·51-58
·
IF 1.9
Xiao-Li Du,Tian-Fang Kang,Li-Ping Lu,Shui-Yuan Cheng
A novel electrochemiluminescence (ECL) biosensor based on CdSe@CdS quantum dots (QDs) functionalized MoS2 modified electrode was developed for sensitive detection of Pb(II) using HRP-mimicking DNAzyme biocatalytic precipitation (BCP) for ECL signal quenching. Polycationic poly(diallyldimethylammonium chloride) (PDDA) and negatively charged QDs were adsorbed on the surface of MoS2, in sequence, to form the MoS2–QDs composites, which were employed as a matrix for the immobilization of the G-rich DNA strand, i.e., T30695. The MoS2–QDs were characterized by UV-vis absorption spectroscopy, fluorescence spectroscopy, energy dispersive spectrometry, transmission electron microscopy, and field emission scanning electron microscopy. CdSe@CdS QDs can produce an ECL signal using 0.1 M K2S2O8 as a co-reactant. T30695 can combine with Pb(II) ions to form a stable parallel G-quadruplex. The G-quadruplex can further combine with hemin to form DNAzyme, which can catalyze H2O2 and oxidize 4-chloro-1-naphthol (4-CN) to produce an insoluble precipitation of benzo-4-chlorohexadienone on the surface of the sensor. When the concentration of Pb(II) in the solution was increased, more of DNAzyme was generated and subsequently more 4-CN molecules were oxidized catalytically, leading to a decrease of the ECL signal. Using this quenching strategy, the ECL signal of the biosensor was linearly dependent on the logarithm of the Pb(II) concentration ranging from 1.0 × 10−15 to 1.0 × 10−11 M with a detection limit of 0.98 f M. The stability and selectivity of the sensor were also investigated. The sensor was applied in the determination of Pb(II) in the real water samples and satisfactory results were obtained.
研究领域:
分析化学
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环境科学
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纳米技术
·
生物传感器
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高影响因子
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