VETERINARY MICROBIOLOGY
·
2023
·
IF 3.3
Lujie Zhang,Xing Liu,Jian Mao,Yangyang Sun,Yanni Gao,Juan Bai,Ping Jiang
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important causative agents in the pig industry worldwide, causing reproductive failure in sows and respiratory problems in growing pigs. Glucose metabolism is a major pathway for energy production and interacts with many cellular processes, such as innate immunity response. It is unclear whether PRRSV infection can use the glucose metabolic pathway to generate immune escape in favor of viral replication. Here, we found that high glucose promotes PRRSV replication and glycolysis, and inhibits poly(I:C)-induced RLR signaling. Conversely, inhibition of the glycolysis pathway significantly promoted poly(I:C)-induced RLR signaling and inhibited PRRSV replication, suggesting that glycolysis promotes PRRSV replication by inhibiting interferon signaling. Furthermore, PRRSV promotes glycolysis to produce lactate, which acts as a key metabolite to promote viral replication by inhibiting RLR signaling by targeting MAVS. And the glycolytic inhibitors targeting HK2 and LDHA in glycolysis could inhibit PRRSV replication. Taken together, these findings suggested that PRRSV infection promotes glycolysis to produce lactate, which targets MAVS to inhibit RLR signaling and thus promote viral replication. Our findings provide an insight into the pathogenesis of PRRSV and offer a theoretical basis for further development of antiviral therapeutic targets.
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 37
·e54417
·
IF 3
Mingjie Lin,Junmei Ma,Xuping Xue,Shiyang Li,Qingyi Wang,Zixuan Lv,Congjie Gao,Linxin Xue
Polyamide (PA) based thin film composite forward osmotic (TFC-FO) membranes were prepared through inter-facial polymerization (IP) processes on porous cellulose acetate (CA) substrates. A dual solvent modification process of adding DMF in the aqueous phase followed by activation with methanol was found to be able to simultaneously improve the permselectivity and anti-fouling capability of the modified membranes. On top of the acidic CA substrates, DMF could not only absorb the HCl formed in the IP processes, but also chemically bind with the PA matrix by reaction with MPD monomers, leading to formation of homogeneous thin and flat PA layers, while methanol activation further swelled the PA matrix, repaired defects and optimized water permeating channels. Using 1 M NaCl solution as the draw solution, the water flux of the CA/PA-DMF4-ME1 membrane was twice that of the pristine membrane, and 24% increase in J w /J s value from 182 to 225 L·mol −1 . Its J w /J s value could be further increased 10 times to 2000 L·mol −1 using 1 M Na 2 SO 4 solution as draw solution. RO tests for the CA/PA-DMF4-ME1 membranes showed 6-fold increase in water permeance, from 1.16 to 7.02 L·m −2 ·h −1 ·bar −1 , as well as increase in NaCl rejection from 94.8% to 97.7% from pristine membrane.
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Journal of Energy Storage
·
2023
·
IF 9.4
Dechao Wang,Hang Zhao,Xun Zhu,Dingding Ye,Yang Yang,Hong Wang,Rong Chen,Qiang Liao
Photo-thermo catalysis represents one of the promising technologies for the use of solar energy to produce hydrogen while recovering chemical energy contained in wastewater. However, inefficient utilization of the whole solar spectrum , high water dissociation activation energy , and costly materials restrict its widespread applications. Herein, we propose to use non-plasmonic, metal-free, cost-effective and full-spectrum-responsive nitrogen-doped carbon materials prepared by using urea as a nitrogen source for photo-thermo catalytic hydrogen production in accompany with aqueous pollutant degradation. It is demonstrated that the used carbon materials can successfully produce hydrogen and decompose pollutants simultaneously via photo-thermo catalytic reactions upon illumination even with actual biogas slurry. Moreover, strong oxidants can be generated via photo-thermo catalysis in the presence of dissolved oxygen, which promotes hydrogen production. Particularly, due to the photothermal conversion, the redox reaction over carbon materials is enhanced and the activation energy is decreased, facilitating hydrogen production. The generated hydrogen radicals promote the regeneration of active sites for thermocatalytic hydrogen production. The synergy of photocatalysis and thermocatalysis leads to the promotion of actual biogas slurry degradation and hydrogen production. This work demonstrates a promising, efficient and cost-effective strategy for simultaneous solar hydrogen production and wastewater treatment .
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Foods
·
2022
·Vol. 11
·期 17
·2660
·
IF 5.561
Xiayu Liu,Xinxin Pang,Yansha Wu,Yajing Wu,Ying Shi,Xinglin Zhang,Qihe Chen
Mannosylerythritol lipids-A (MEL-A) is a novel biosurfactant with multiple biological effects. The synergistic antibacterial activity and mechanism of MEL-A and lactic acid (LA) againstListeria monocytogeneswere investigated. The synergistic effect resulted in a significant increase in the antibacterial rate compared to LA treatment alone. Genome-wide transcriptomic analysis was applied to deeply investigate the synergistic antibacterial mechanism. Gene Ontology (GO) enrichment analysis showed that the synergy between MEL-A and LA affected many potential cellular responses, including the sugar phosphotransferase system, carbohydrate transport, and ribosomes. KEGG enrichment analysis showed that the PTS system and ribosome-related pathways were significantly enriched. In addition, synergistic treatment affected locomotion and membrane-related cellular responses in GO enrichment analysis and carbohydrate metabolism and amino acid metabolism pathways in KEGG enrichment analysis compared to LA treatment alone. The accuracy of the transcriptome analysis results was verified by qPCR (R2= 0.9903). This study will provide new insights for the prevention and control ofL. monocytogenes.Keywords:Mannosylerythritol lipids-A (MEL-A);lactic acid;Listeria monocytogenes;RNA-Seq
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ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Lijuan Liu,Benlong Su,Qifeng Wei,Xiulian Ren
Nowadays, the development of appropriate technologies is of great significance for society. To avoid the negative impact of traditional separation methods on the environment, novel biodegradable amides were used for the separation of lactic acid (LA) by the in situ formation of deep eutectic solvents (DESs). The formation mechanism was explained by quantum chemical calculations and FT-IR analysis. In addition, the stripping of LA was performed by hydrophobic DESs interacting with water, and the amide in DESs was regenerated and reused. Meanwhile, the classical molecular dynamics simulations were adopted to describe the complex mixture of the amide–LA–water system structures and properties in depth. The essence of interaction between hydrophobic DESs and water is a dynamic reversible phase equilibrium process. This study provides a new direction for the development of clean technology such as carboxylic acid separation and also provides guidance for the design of hydrophobic DESs.
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Nanoscale
·
2022
·Vol. 14
·期 46
·17222-17229
·
IF 8.307
Heng Lu,Xiaohong Tan,Guobin Huang,Shaoru Wu,Yanmei Zhou,Junying Zhang,Qiaowen Zheng,Tianju Chen,Feiming Li,Zhixiong Cai,Jingbin Zeng,Maosheng Zhang
Perovskite nanocrystals (PNCs) have attracted widespread attention as promising materials for the optoelectronic field due to their remarkable photophysical properties and structural tunability. However, their poor stability and the use of toxic organic solvents in the preparation process have severely restricted their practical applications. Herein, a facile, rapid and toxic organic solvent-free synthesis strategy of CsPbBr3 PNCs was developed for the first time via the ligand-assisted reprecipitation (LARP) method using natural deep eutectic solvents (NADESs) as solvents and surface ligands. In this method, the NADESs not only functioned as solvents for green synthesis, but also served simultaneously as surface ligands of CsPbBr3 PNCs to significantly improve their optical properties and stability. The as-synthesized CsPbBr3 PNCs exhibited high photoluminescence quantum yield (PLQY, ∼96.8%), narrow full width at half-maximum (FWHM, ∼18.8 nm) and a high stability that retained 82.9% of PL intensity after 70 days. This work provides a new strategy for the green synthesis of PNCs, which promises feasibility for the industrial large-scale synthesis of high-quality PNCs.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Lijuan Liu,Hui Fang,Qifeng Wei,Xiulian Ren
Carboxylic acids are important precursors of chemical raw materials produced from the bioconversion of renewable feedstocks. In these processes, the separation of carboxylic acid products from fermentation broth is of great significance and challenge. In this research, the extraction performance of carboxylic acid using novel hydrophobic deep eutectic solvents (DESs) composed of amides and geraniol was studied. Molecular dynamics (MD) simulations have also been adopted to understand the extraction mechanism. Radial and combined distribution functions analysis shows that hydrogen bond-induced geraniol-carboxylic acid and amide-carboxylic acid multi-molecular assembly drives the separation of carboxylic acids from fermentation broth. Thermodynamics and mean square displacement calculations show that increasing temperature breaks the hydrogen bond networks, and the hydrophobic DESs can be recycled. Finally, a mini-pilot experiment for lactic acid recovery from fermentation broth verified the effectiveness of the amide-based DESs and their application to practice. These findings provide references for industrial-scale production of carboxylic acid separation.
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Chenkai Cao,Quan Yuan,Cong Wang,Lifeng Deng,Huijun Li,Ding Wang
The detection of acetone is of great significance for environmental safety and human health. Micromorphology control is a feasible pathway to obtaining excellent gas-sensitive materials. In this study, three types of hierarchical WO 3 ·H 2 O, including nanoplates, hollow spheres and nanospheres, were controlled synthesis by a simple hydrothermal method. Their morphologies and growth mechanisms were studied by various characterization methods, and their acetone sensing performances were tested via a static distribution method. The results indicate that the sensor based on the hierarchical WO 3 ·H 2 O hollow spheres exhibits higher sensitivity, better selectivity and faster response speed towards acetone than two other morphologies at the optimum operating temperature (300 ℃). Specifically, the response value reaches 21.4–50 ppm acetone, and the response/recovery times are 3.6 s/6.0 s. The superior performance and fast response characteristics of the hollow WO 3 ·H 2 O are mainly attributed to its high specific surface area, unique pore structure, abundant surface active sites, and suitable energy level structure. This work enlightens some thoughts on the functionalization of tungsten-based gas sensors and efficient detection of acetone.
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ACS Sustainable Chemistry & Engineering
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2022
·
IF 9.224
Zixuan Fan,Hao Sun,Luoyuan Zhang,Xin Zhao,Yingcheng Hu
Wood is a green, environmentally sustainable, high-performance structural material that is an attractive candidate to replace nonenvironmental structural materials. However, the mechanical performance of natural wood is generally insufficient in many advanced engineering structures and applications. Herein, we report a simple and effective strategy in a new type of green-enhanced wood development to synthesize a high mechanical biomass structure material from renewable wood resources. In this process, the lignin of the natural wood is partially removed, and the hard cell walls become pliable and easy to handle. After treatment, the wood fiber structure is preserved, and the wood fibers are closely arranged after densification, which shows the reinforced wood with high mechanical properties. The resulting reinforced wood is 8 times stronger than the starting wood and comparable to widely used structure materials such as concrete, stainless steel, aluminum alloy, and magnesium alloy. This method of manufacturing reinforced wood with green, rich, and renewable natural wood is extremely attractive for the sustainable development of engineering structural materials, which broadens the potential of wood as structural materials.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Jiashu Liu,Shengnan Zhao,Song Song,Haifeng Cong,Wei Luo,Tong Ding,Ye Tian,Xingang Li
One-step lactide (LD) synthesis from lactic acid (LA) is a promising method for an economically viable polylactic acid (PLA) industry. However, this synthesis is based on a highly water-sensitive esterification reaction, and few studies focus on developing an efficient method to improve its activity by water removal from the reaction environment. Herein, we report a new strategy coupling salt-promoted toluene–water phase separation with heteroazeotropic distillation to enhance water removal to realize highly efficient LD synthesis. Different kinds of chloride salts (LiCl, NaCl, CsCl, MgCl2, and CaCl2) were selected to study their influence on the reaction. The highest yield of LD (84.7%) was obtained when CaCl2 was added, much higher than that of the salt-free reaction (72.3%). Hydrated Ca2+ cations with relatively large charge number and small radius can efficiently reduce the solubility of water in toluene due to their strong electrostatic force to attract more water molecules. Mechanism studies demonstrate that the increased yield of LD after adding CaCl2 was attributed to its significant inhibition of LD hydrolysis resulting from the deep water removal from the toluene solvent.
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Foods
·
2022
·Vol. 11
·期 13
·1920
·
IF 5.561
Qi Quan,Wei Liu,Jiajing Guo,Meiling Ye,Juhua Zhang
Six lactic acid bacteria strains were used to study the effects on physicochemical characteristics, antioxidant activities and sensory properties of fermented orange juices. All strains exhibited good growth in orange juice. Of these fermentations, some bioactive compositions (e.g., vitamin C, shikimic acid) and aroma-active compounds (e.g., linalool, 3-carene, ethyl 3-hydroxyhexanoate, etc.) significantly increased inLactiplantibacillus plantarumandLactobacillus acidophilussamples. DPPH free radical scavenging rates inL. plantarumandLacticaseibacillus paracaseisamples increased to 80.25% and 77.83%, respectively. Forty-three volatile profiles were identified, including 28 aroma-active compounds. 7 key factors significantly influencing sensory flavors of the juices were revealed, including D-limonene, linalool, ethyl butyrate, ethanol,β-caryophyllene, organic acids and SSC/TA ratio. The orange juice fermented byL. paracasei, with more optimization aroma-active compounds such as D-limonene,β-caryophyllene, terpinolene andβ-myrcene, exhibited more desirable aroma flavors such as orange-like, green, woody and lilac incense, and gained the highest sensory score. Generally,L. paracaseifermentation presented better aroma flavors and overall acceptability, meanwhile enhancing antioxidant activities.Keywords:orange juice;lactic acid fermentation;physicochemical characteristics;antioxidant activities;volatile profiles;sensory properties
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Fermentation-Basel
·
2022
·Vol. 8
·期 6
·262
·
IF 5.123
Jun Hao,Wen-Tao Sun,Chang-Rong Wu,Ming-Zhu Zhang,Guang-Hao Xia,Yu-Long Zheng,Chao Chen
Broussonetia papyriferaL. (paper mulberry) is an alternative woody plant, which can used to replace part of the protein feed for ruminants. Ensiling is an effective way to preserve fresh pasture and to solve the problem of stable storage and feed conversion of paper mulberry in the rapid growth period. However, low dry matter (DM), water-soluble carbohydrate, and lactic acid bacteria (LAB) reduce the quality of paper mulberry silage. This study assesses the influence of wilting time (0 h and 3.5 h; lighting: 3.43 × 104Lux) and three additives (Enterococcus durans, CL; cellulase, CE; and formic acid, FA) on the fermentation quality, aerobic stability, and bacterial community of whole plantB. papyriferasilage. The whole plantB. papyriferasample was mowed and wilted for 0 h and 3.5 h, and then had CL, CE, or FA added, followed by 60 days of ensiling. The results show all silage samples had high fermentation quality with pH below 4.2, ammonia-nitrogen below 100 g/kg DM, and no detectable butyric acid. The additives protected the DM and the crude protein from protease activity (p< 0.05), and CL was the most effective among them. Furthermore, wilting time influenced the silage’s bacterial communities, but overall, CL treatment had the greatest impact on bacterial communities. Wilting time and formic acid treatment significantly improved aerobic stability (p< 0.05).Enterococcuswas positively correlated with lactic acid (LA), while negatively correlated with LA andWeissella(p< 0.001). Enterococcuswas identified as the main driver of the whole plant paper mulberry ensiling process in the present study. In conclusion, compared to other additives, LAB is the most effective and economical to improve the fermentation quality and reduce the protein degradation of whole plant paper mulberry silage. Our findings provide a theoretical basis to improve the quality and production of paper mulberry silage.Keywords:paper mulberry;silage;wilting;additives;microbial community;aerobic stability
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