MOLECULES
·
2025
·Vol. 30
·期 5
·998
·
IF 4.6
Yuanqi Duan,Jinfeng Sun,Yongkang Xue,Weiwei Xu,Yuxin Jiang,Tieqiang Zong,Wei Zhou,Zhengyu Hu,Gao Li
Given that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques were used to compare structural characteristics and morphology. Neuroprotective effects were explored using OGD/R-induced PC12 cells. The results showed that BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a had similar characteristic groups and contained both β-glycosidic and α-glycosidic bonds. Their molecular weights, in descending order, were 198.398 kDa, 184.690 kDa, 184.556 kDa, and 184.217 kDa, respectively. In addition, the four polysaccharides contained different proportions of glycosidic bonds, namely, Manp-(1→, →5)-Araf-(1→, →3)-Galp(or GalAp)-(1→, →4)-Glcp-(1→ and →3,6)-Galp-(1→. BCP30-1a also contained a certain proportion of Galp-(1→, and each polysaccharide had different microscopic characteristics and good thermal stability. Finally, BCP50-1a, BCP70-1a, and BCP90-1a exhibited good cytoprotective effects on PC12 cells based on the OGD/R model. These findings provide a novel regulatory strategy for the functional activity of BCPs and offer scientific evidence supporting application in the research field of ischemic stroke.
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DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Zuoying Wang,Sida Zhang,Yue Li,Zhuoshuo Zhou,Xu Zhang,Ying Chen,Yanli Zhao,Cai Ye,Jinlian Li,Naitian Zhang,Dongmei Wu
The fibrous roots of Atractylodes chinensis are rich in polysaccharides, however, the structure and application of its polysaccharides are still unclear. In this study, a 3.7 kDa inulin-type fructan was purified, which was named ACFP-1. Structural characterization indicated that ACFP-1 was comprised of fructose, arabinose, glucose and galactose. The main chain was →1)- β -Fruf-(2 → 1)- β -Fruf-(2 → linkage type. The branch chain was α -Araf-(1 → 5)- α -Araf-(1 → linkage type, which was connected to the main chain through O-6 of →1,6)- β -Fruf-(2 → glycosidic bond. Activity analysis indicated that ACFP-1 inhibited the generation of ROS in HaCaT cells and AB strain zebrafish. ACFP-1 reduced the positive rate of β-galactosidase and the expression of p53 and p21 protein, restored oxidative stress-induced autophagy inhibition, promoted the expression of LC3II protein, and inhibited the expression of p62 protein in HaCaT cells. These experiments demonstrated antioxidant, anti-aging activity and activation of autophagy of ACFP-1, and ACFP-1 could be used as a potential antioxidant.(补充).
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongchun Huang,Jie Bai,Jinman Shui,Zhanhai Su,Chengzhu Cao,Haiyan Wang,Qiong Wu,Shoude Zhang
Analysing monosaccharide composition is an essential step in deciphering polysaccharide structures. However, it presents significant challenges due to the pronounced hydrophilicity and the vast array of monosaccharide isomers. Gas/liquid chromatography, coupled with an aldononitrile acetate derivatisation, has been widely used for monosaccharide composition analysis. However, it poses considerable difficulties in simultaneously determining the D/L configurations of these monosaccharides. In this study, a novel method that can simultaneously determine the composition of polysaccharides and their D/L configuration based on L-cysteine methyl ester derivatisation (CMED) and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the comprehensive analysis of monosaccharide types. This method enables the simultaneous characterization of aldoses, ketoses, alditols, and uronic acids. Furthermore, the reaction mechanisms and by-products were meticulously analysed, offering a robust theoretical basis for this novel methodology. Additionally, the developed method was employed to characterize the monosaccharide composition for six plant polysaccharides. Ultimately, the findings demonstrated that the proposed method is effective for confirming monosaccharide composition, encompassing both structural types and absolute configurations.
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高影响因子
危险品化学品经营许可证（带存储）