JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Chenxi Liu,Jihan Huang,Sinuo Cheng,Huiyuan Tang,Xizhi Shi,Bo Liu,Wang Li,Bo Zhou,Jiali Ren
Detection of 3-MCPD in lipid-rich food matrices remains challenging due to trace analyte levels, high viscosity, and severe matrix interference. Herein, a photothermal-driven magnetic “three-in-one” sensing platform based on L -cysteine-functionalized ZnFe 2 O 4 ( L -ZFO) nanoparticles was developed for rapid and sensitive screening of 3-MCPD in edible oils. The multifunctional platform integrates magnetic enrichment, photothermal-assisted signal amplification, and colorimetric sensing, enabling efficient extraction and detection with minimal sample pretreatment. Benefiting from the synergistic interfacial enrichment and photothermal enhancement, the proposed method exhibited a linear range of 0.01–0.20 mg/L with a low detection limit of 1.4 μg/L. The platform showed acceptable recoveries of 75.0–92.0% and relative standard deviations below 5.8% in edible oil samples. Moreover, comparable responses obtained in buffer and oil matrices demonstrated excellent anti-interference capability toward complex lipid systems. The recyclable L -ZFO probe also exhibited good stability and reproducibility over 3 cycles. This work provides an elimination of pretreatment strategy for rapid on-site screening of 3-MCPD in edible oils.
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DIAMOND AND RELATED MATERIALS
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2026
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IF 5.2
Lu Sun,Jie Wei,Qing Hu,Xiuquan Xu,Jing Wu,Yaoyao Cao,Yao Zhang,Haoyan Wu
A Fe/V-CN nanozyme with synergistically enhanced POD-like activity was rationally designed. • A sensitive cascade colorimetric platform for detecting gastric cancer-related D-amino acids was successfully constructed. • The assay supports reliable quantification of D-amino acids in complex human saliva matrices. The development of a practical and reliable cascade colorimetric method for detecting D-amino acids (DAAs) provides a promising tool for the prevention and preliminary diagnosis of gastric cancer. Herein, a bimetallic Fe V supported on graphitic carbon nitride (Fe/V-CN) nanozyme was rationally designed and synthesized via a simple annealing strategy. The atomic dispersion of Fe and V species, along with their coexisting multivalent states (Fe 2+ / 3+ , V 4+ / 5+ ) synergistically boosted the intrinsic peroxidase-like (POD) activity to Fe/V-CN, which were characterized by a high substrate affinity ( K m of 0.078 and 0.083 mM for TMB and H 2 O 2 ) and an accelerated catalytic turnover ( V max of 4.94 × 10 −7 and 7.76 × 10 −7 M·s −1 , respectively). Leveraging this improved activity, a cascade colorimetric sensing system was developed by integrating Fe/V-CN with D-amino acid oxidase (DAAO) for the specific detection of DAAs. The platform exhibited favorable analytical performance for D-proline (D-Pro) and D-alanine (D-Ala), offering wide linear ranges of 0–200 μM and 0–160 μM and low detection limits of 0.93 μM and 1.45 μM, respectively, combined with acceptable selectivity against common interferents. For real-sample analysis, the platform was successfully implemented in determine DAAs in human saliva, delivering satisfactory recoveries (95.8–105.7%) and good precision (RSD < 3.5%). This work presented a synergistic Fe/V bimetallic nanozyme and a practical sensing strategy, demonstrating the feasibility of DAAs detection in complex biological matrices. Download: Download high-res image (70KB) Download: Download full-size image
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·506
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IF 5.4
Jiang Xue,Zheng Yuxin,Arkin Kamile,Xu Yuting,Li Kexin,Shang Qingkun
Blue fluorescent carbon dots (Soy-His CDs) with high fluorescence quantum yield were synthesized via a hydrothermal method using biowaste soybean straw and L-histidine as precursors. A ratiometric fluorescence probe (Soy-His CDs@PEp-PEI) was subsequently constructed for the visual detection of epinephrine (Ep). Upon the continuous formation of the PEp-PEI copolymer, the green fluorescence of the Soy-His CDs@PEp-PEI probe at 515 nm gradually intensified, while the blue fluorescence at 420 nm was progressively quenched. This allows for the rapid and sensitive detection of Ep by monitoring the variation in the I 515 /I 420 ratio. The limit of detection (LOD) was determined to be 0.025 µmol/L in the linear range 0.1–20 µmol/L. The response mechanism is attributed to the inner filter effect (IFE) between the Soy-His CDs and the polyepinephrine-polyethyleneimine (PEp-PEI) copolymer. The proposed method was successfully applied to detect Ep in human urine samples, yielding recoveries ranging from 95.82% to 102.01%. Furthermore, portable visual and intelligent detection of Ep was achieved using a smartphone equipped with RGB analysis software based on the Soy-His CDs@PEp-PEI probe system, with recoveries ranging from 94.65% to 108.33%. A good linear relationship was obtained in the range 0–10 µmol/L, with a limit of detection (LOD) of 0.14 µmol/L. Graphical
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Advanced Optical Materials
·
2026
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IF 7.2
Xuetao Yan,Lifei Chen,Yuze Ren,Kaixuan Cui,Tianliang Li,Lixing Lin,Zeyu Li,Haicheng Song,Yingying Chen,Zhenzhen Li,Lingyan Feng
Circularly polarized luminescence (CPL) materials are highly sought after for their unique chiroptical properties, but their rational design remains challenging. To address this, a machine learning (ML) platform that intelligently screens deep eutectic solvents (DES) for crafting G-quartet-based gels with tailored CPL is established. An ML classifier trained on experimental data predicts CPL activity with 0.916 accuracy. SHapley Additive exPlanations (SHAP) analysis identifies key DES molecular descriptors, guiding the discovery of novel materials that exhibit a record-high luminescence dissymmetry factor (g lum ) of 0.222 for nucleotide-based systems and achieve chirality inversion via DES modulation. The Sure Independence Screening and Sparsifying Operator (SISSO) algorithm further yields interpretable design formulas. Leveraging the distinct polarization of these gels, we demonstrate a fourth-level anti-counterfeiting strategy, decrypting concealed information via Morse and ASCII codes to enhance information security. This ML-driven methodology accelerates the discovery of high-performance CPL materials and opens new design avenues.
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MICROCHEMICAL JOURNAL
·
2026
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IF 5.2
Yuying Han,Xingyu Hou,Rui Hu,Xiaopeng Yang,Lin Hou,Xue Sun,Yuguang Lv,Lixin Ding,Ze Li
A straightforward yet sensitive dual-signal strategy was developed for the detection of melatonin (MT), based on the fluorescence properties and peroxidase-like activity of zinc‑nitrogen co-doped carbon dots (Zn,N-CDs). Through hydrogen bonding, electron transfer, as well as coordination interactions with MT, Zn,N-CDs undergo fluorescence quenching driven by the synergistic effects of static and dynamic mechanisms. Meanwhile, the generated singlet oxygen oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into the blue-colored oxidized product (oxTMB), thus producing a ‘triggered’ dual-signal response. This platform enables simultaneous fluorescence and colorimetric analyses, offering wide linear ranges (fluorescence: 2–600 μM; colorimetry: 5–500 μM) and remarkably low detection limits (0.020 μM and 0.999 μM, respectively). Furthermore, the method exhibits excellent selectivity and has been successfully applied to pharmaceutical sample analysis, highlighting its promise for visual quality monitoring of relevant products.
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TALANTA
·
2026
·
IF 6.7
Haoran Li,Shijun Xiong,Beibei Chen,Zhekuan Wu,Zhen Zhou,Yong Liang,Man He,Bin Hu
Amadori and Heyns compounds are key Maillard reaction intermediates, resulting from the reaction of a certain amino acid with glucose (Glu) or fructose (Fru), respectively. They play a dual role in augmenting cigarette aroma and attenuating sensory irritation, and their quantification in tobacco is vital for tobacco quality evaluation and Maillard reaction mechanism study. Currently, simultaneous quantification of Amadori and Heyns compounds derived from the same amino acid, which present as isomers, is encumbered by poor chromatographic separation, and reliance on unavailable specific fragment ions. Herein, a method based on high performance liquid chromatography combined with tandem mass spectrometry (HPLC-MS/MS) detection was developed for the simultaneous quantification of Amadori and Heyns compounds derived from the same three amino acid (alanine, proline, phenylalanine, abbreviated as Ala, Pro, and Phe, respectively) with an ion ratio strategy. Under the optimized conditions, the limits of detection ranging from 2.22 to 6.94 ng/mL were obtained for six target analytes (Fru-Phe/Glu-Phe, Fru-Pro/Glu-Pro, Fru-Ala/Glu-Ala). When the method was applied to tobacco leaf powder extracts, target Amadori and Heyns compounds were found in the range of 0.65-56.4 μg/g, with Heyns compounds consistently less abundant than Amadori counterparts. In the recovery test, target analytes showed recoveries of 76.8-111%. Compared with reported methods, the proposed method features universal applicability without requiring characteristic fragment ions or chromatographic separation. It provides a reliable tool for analyzing hard-to-separate sugar-derived Maillard intermediates in tobacco.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
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Food Chemistry-X
·
2026
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IF 9.2
Wenxin Chen,Jiahao Fu,Yaqi Liu,Yao Fan,Haiyan Fu,Yuanbin She
Ochratoxin B (OTB) that widely presented in food has significant cytotoxicity even at low concentration levels. Herein, a novel fluorescent sensor based on the 2-mercapto-5-benzimidazole carboxylic acid (MBI)-capped CdSe quantum dots (QDs) was established for the selective detection of OTB in food. Specifically, the synergistic effect of electrostatic interaction and intermolecular hydrogen bonds between MBI and OTB provided a direct and sensitive fluorescence signal that enables efficient recognition of OTB from other mycotoxins, even OTA with similar structures. Excellent linear detection performance for OTB is exhibited with a range from 0.037 ng/mL to 48.01 ng/mL and a detection limit of 0.016 ng/mL. Furthermore, our developed method exhibits good anti-interference stability against contaminants such as mycotoxins, inorganic salts and amino acids. Most importantly, the sensor demonstrates high sensitivity in real food samples with the recovery ranges from 96.51% to 104.7%, with an RSD of less than 2.67%.
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ULTRASONICS SONOCHEMISTRY
·
2026
·
IF 10.2
Yangyang Zhang,Weifeng Jin,Tianxue Chen,Jin Han,Li Yu,Haitong Wan,Haofang Wan
This study developed an efficient and sustainable extraction process for extracting ferulic acid (FA) and ligustilide (LIG) from Angelica sinensis Radix (ASR) using green natural deep eutectic solvents (NADESs) by machine learning optimization. After screening, L-proline/methylurea was identified as the optimal NADES system, and the genetic algorithm optimized back-propagation neural network (GA-BPNN) model demonstrated superior predictive performance over traditional methods (MSE = 1.7 × 10 5 , R 2 = 0.9860). Under the optimized conditions (NADES volume fraction 67.44%, ultrasonic time 25.556 min, ultrasonic temperature 59.791 ℃, and solid–liquid ratio 7.610 mg/mL), the predicted extraction yield was 0.149, which closely matched the experimental yield of 0.151. Shapley Additive Explanations (SHAP) analysis further indicated that the NADES volume fraction was the most critical factor affecting extraction efficiency. Animals’ experiments demonstrated that both FA and LIG significantly ameliorated neurological deficits and reduced cerebral infarct volume in middle cerebral artery occlusion/reperfusion (MCAO/R) mice. To further elucidate the underlying antioxidant mechanism, Mendelian randomization (MR) analysis was employed, revealing a significant causal relationship between FA and superoxide dismutase (SOD) expression (P = 0.0265), but no significant association with heme oxygenase-1 (HO-1); LIG showed no significant association with either. These findings were corroborated in MCAO/R mice, FA administration significantly increased SOD levels in plasma. Collectively, the GA-BPNN-guided NADES combined with ultrasound assisted extraction (UAE) strategy significantly enhanced the extraction of bioactive compounds from ASR. Moreover, the integrated approach combining MR analysis with in vivo experiments provided converging evidence supporting the underlying specific antioxidant mechanism mediated by FA. Download: Download high-res image (169KB) Download: Download full-size image This study developed an efficient extraction process for ASR using NADES combined with ultrasonic assistance, optimized via machine learning models, and evaluated the antioxidant activities of FA and LIG in relation to SOD and HO-1 expression.
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Advanced Sensor and Energy Materials
·
2026
·
IF 12.3
Tingting Wang,Hao Song,Xiaohui Gong,Zongyu Han,Taoming Xu,Yanan Xu,Dan Zhao,Chuanxia Chen,Yizhong Lu,Jian Sun
Biothiol analysis is crucial for evaluating redox homeostasis and monitoring various physiological processes. However, conventional fluorescence-based strategies often rely on structurally complex probes and lack straightforward multi-target discriminative capabilities. Herein, we present a versatile, pre-synthesis-free fluorogenic platform driven by the target-responsive inhibition of in-situ polymer dot (PD) formation. We discovered that dopamine (DA) spontaneously condenses with N -[3-(trimethoxysilyl)propyl]diethylenetriamine (AEEA) to yield robustly fluorescent PDs. Biothiols, acting as highly reactive competitors, intercept the DA precursor via a preferential Michael addition, fundamentally suppressing PD formation and inducing a marked fluorescence decrease. This mechanism enables the rapid, highly sensitive quantification of glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), with low limits of detection (LODs) of 0.095, 0.115, and 0.083 μM, respectively. Significantly, the introduction of a structurally distinct DA-DAMO analog facilitated the discrimination of these biothiols. This reaction-interference strategy was then successfully coupled with enzymatic cascades to evaluate glutathione reductase (GR) activity within 20 min, achieving a LOD of 0.046 mU/mL. The programmable analyte-dependent fluorescence outputs were successfully employed as signal readouts to construct molecular logic gates. This work establishes target-regulated in-situ nanomaterial formation as a powerful paradigm for biothiol sensing, enzyme assays, and advanced logic operations.
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BMEMat
·
2026
·
IF 17.2
Siyu Pan,Xuanyi Lu,Wenjie Yang,Yunhui Peng,Lingfu Deng,Shuai Zha,Xueyang Fang,Guochen Bao,Lijun Jiang
Intratumoral L-lactate represents a valuable biomarker for both prognosis and therapy response, underscoring the importance of its accurate detection. However, traditional L-lactate assays rely on indirect hydrogen peroxide readouts and often compromise the accuracy and specificity. Herein, we report an aptamer-based nanoprobe (UCNP-Apt-Act) that allows direct L-lactate detection. Aptamers offer high specificity for L-lactate but suffer from poor cellular uptake and premature activation by extracellular L-lactate. To overcome this, we introduced lanthanide-doped upconversion nanoparticles (UCNPs) to enable spatially controlled unlocking by near-infrared (NIR) light for intratumoral L-lactate detection. The nanoprobe remains “locked” during delivery via an engineered actuator, preventing premature response to extracellular L-lactate. Upon 980 nm excitation, UCNPs convert NIR to UV emission, photocleaving the actuator linker and unlocking the aptamer. Notably, the UCNP carriers enhanced cellular uptake by 41 times through overcoming membrane barriers. The unlocked nanoprobe shows a 46-fold fluorescence enhancement in the presence of L-lactate, with an exceptional selectivity against a broad range of molecules and biological ions and metabolites. It enables specific detection of L-lactate in cancer cells, colorectal tumors, and diverse real samples, with excellent recoveries of 95.0%–110.0%. This strategy establishes a robust approach for endogenous small-molecule sensing and provides a versatile platform for biomedical applications.
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JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Min Zhou,Rusen Miao,Xiaoxu Chen,Anqi Wang,Xueman Wei,Initizar Anwar,Yuefei Wang,Hongyan Wang,Fei Tian
A hydrophilic SUPRADES was constructed for selective extraction of isoflavone from P. lobata root. • The SUPRADES achieved 70.4 mg/g puerarin, 1.33–5.33-fold higher than conventional methods. • Selective extraction mechanism was elucidated by FT-IR, ¹H NMR, and DFT theoretical calculations. • The SUPRADES retains >97% extraction capacity over 7 cycles with superior batch reproducibility. • GAPI evaluation verifies the eco-sustainability of the entire isoflavone extraction process. Supramolecular deep eutectic solvents (SUPRADES) have are promising green extraction media for the sustainable extraction of bioactive compounds from plant materials. In this study, a combined strategy of SUPRADES with ultrasound-assisted extraction (UAE) was developed for the efficient extraction and enrichment of isoflavones from the root of Pueraria lobata. The extraction process was systematically optimized using single-factor experiments followed by Box–Behnken design (BBD) response surface methodology. The optimal SUPRADES system consisted of L‑proline and urea at a 1:2 molar ratio, supplemented with 5 wt% β‑cyclodextrin and 30 wt% water. Under these conditions, the yield of puerarin reached 70.4 ± 0.6 mg/g, which is 1.33‑ to 5.33‑fold higher than those obtained with conventional methods. The self‑assembled structure of the SUPRADES and its molecular‑level interaction mechanism with isoflavones were further elucidated by FT‑IR spectroscopy, ¹H NMR spectroscopy, and density functional theory (DFT) calculations. These results confirmed that the extraction process is synergistically driven by hydrogen bonding and β‑cyclodextrin‑mediated host–guest complexation. GAPI evaluation demonstrated the excellent environmental friendliness and sustainability of the established method. Collectively, this study provides a novel and efficient extraction strategy for isoflavones from Pueraria lobata root and offers a theoretical basis for green the extraction of natural products in traditional Chinese medicine. Download: Download high-res image (408KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）