CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Keguang Mu,Dalun Zhang,Ziqiang Shao,Dujian Qin,Yalong Wang,Shuo Wang
l -Dopa functionalized halloysite nanotubes (HNTs) were prepared by the self-polymerization of l -dopa in the weak alkaline condition. Then different contents of l -dopa coated HNTs (LPDHNTs) were blended into cellulose acetate to prepare enhanced performance ultrafiltration membranes via the phase inversion method . The HNTs and LPDHNTs were characterized by FTIR, XPS, and TEM anysis. And the membranes morphologies, separation performance, antifouling performance, mechanical properties and hydrophilicity were also investigated. It was found that the composite membranes exhibited excellent antifouling performance. The pure water flux of 3.0 wt% LPDHNTs/CA membrane increased from 11.4 L m −2 h −1 to 92.9 L m −2 h −1 , while the EA rejection ratio of the membrane was about 91.2%. In addition, the mechanical properties of the resultant membranes were strengthened compared with the CA ultrafiltration membrane.
研究领域:
材料科学
·
高分子科学
·
膜技术
·
生物医学工程
·
环境功能材料
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DOI
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 2
·e202203646
·
IF 2.1
Siying Ren,Wen-Chao Geng,Xuexian Cui,Bian Wu,Zhe Zheng
Graphical We present a robust supramolecular tandem assay to monitor L-DOPA decarboxylase activity and screen enzyme inhibitors using cucurbit[7]uril and acridine orange reporter pair. L–DOPA decarboxylase (DDC) deficiency causes serious progressive neurodegenerative disorders. Developing a sensitive enzyme activity monitoring and inhibitors or activators screening approach is highly on demand for DDC deficiency diagnosis. Here, we report a simple and sensitive fluorescence supramolecular tandem assay based on reporter pair of cucurbit[7]uril and acridine orange for DDC activity monitoring continuously. Furthermore, this strategy demonstrates the potential of STA to screen inhibitors of DDC.
研究领域:
生物化学
·
医学诊断
·
药物开发
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yile Hu,Hui Yang,Wei Li,Keke Du,Simin Song,Wenhui Zhang,Zhiqiang Wei,Sanqiang Li
In current work, the highly sensitive screen-printed electrochemical sensor for epinephrine (EP) detection was constructed based on reduced graphene doped poly (L-dopa)/PEDOT:PSS composites by the simple solution coating method and electropolymerization. The constructed sensor exhibited excellent sensor performances and recorded the linear response of EP in the concentration range of 0.10–16.50 μM with detection limit of 0.02 μM. The practical analytical utility provides great promise for sensitive measurements of EP in human serum, and has broad prospects in clinical and pharmaceutical applications.
研究领域:
电化学
·
生物传感器
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材料科学
·
分析化学
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 10
·1963-1970
·
IF 5.687
Dedai Lu,Yongyong Zhang,Ting'e Li,Yunfei Li,Hongsen Wang,Zhiqiang Shen,Qiangbing Wei,Ziqiang Lei
The incorporation of L-3,4-dihydroxyphenylalanine (L-DOPA) residues in mussel-like biomimetic copolymers has drawn great attention due to their adhesive properties. In this work, a series of temperature-sensitive and hyperbranched poly(amino acid)s containing different functional side groups (catechol, guanidyl, mercapto and double bond) were designed and synthesized by the ring-opening polymerization of N-carboxy-α-amino acid anhydride (NCA), using a temperature-sensitive and multi-hydroxyl end pluronic L-31 as the initiator in this process. The tissue adhesive properties of these copolymers were evaluated by tensile strength tests on wet pork skin. We found that the topological structure, side groups of copolymers, adhesive temperature and cure time had influence on the wet adhesive strength, especially side groups. While only L-DOPA was incorporated into copolymeric chains, the wet adhesive strength was about 106 kPa. With guanidyl, mercapto and double bonds incorporated into copolymeric chains, an obvious increase in the wet adhesive strength was observed. Additionally, the wet adhesive strength improved by about 20 kPa at body temperature (37 °C) as compared to that at room temperature (25 °C). Meanwhile, we found that it has good biocompatibility and degradability through its cytotoxicity and degradation tests.
研究领域:
生物材料学
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高分子科学
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组织工程
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再生医学
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生物医学工程
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药物递送
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表面科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xin Zhou,Bowen Li,Mingming Guo,Wenchang Peng,Deping Wang,Qinglu Guo,Shuchao Wang,Dong Ming,Bin Zheng
Controlling medication according to the condition has become a universal treatment regimen for Parkinson's disease (PD) worldwide. However, the general oral administration method is prone to a variety of digestive system discomfort, easy to be broken down by digestive juices and intestinal microorganisms, resulting in low absorption efficiency, and a serious delay in the efficacy of the drug. Here, we developed a microneedle patch (MNP) that could release drugs with controlled temporal and spatial precision triggered by near-infrared (NIR) light for the treatment of PD. The microneedles (MNs) took Gelatin-methacryloy (GelMA) as the matrix, and therapeutic drugs (L-DOPA) were stored in the mesopore of the upconversion micron-rods (UCMRs), dispersing in the GelMA. The molecular motor, as a poreblocker and gate switch, isomerizes under the excitation of ultraviolet and visible light from UCMRs for controlling drug release. This kind of MNP penetrates the epidermis in a painless, non-invasive, and non-infectious way. Under the exposure of NIR light, the L-DOPA in the MNs will be released as needed, and then it seeps into the blood and brain for relieving the symptoms of PD. In the treatment of Parkinson's mice model, this regimen significantly restored motor function, and further mechanistic studies revealed that it reduced neuroinflammation and dopaminergic neuronal death in the substantia nigra. Besides, the released L-DOPA directly entered the blood, which reduces the side effects on the gastrointestinal tract and improves the utilization rate of the drug. These attractive superiorities indicated that the MNP with controlled drug delivery system may be candidate strategies for the treatment of PD and can be used in various related biomedical fields.
研究领域:
药学
·
神经科学
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hengrui Liu,Xiaorong Yang
L-DOPA (3,4-dihydroxyphenyl-L-alanine) is a key drug for the Parkinson's disease. The global demand of L-DOPA is continuously increasing. Tyrosine phenol-lyase (TPL) is the environmentally friendly biocatalyst for L-DOPA biosynthesis. In this study, a novel TPL from Mycobacterium tuberculosis (Mt-TPL) was identified and expressed in Escherichia coli . The characterization of Mt-TPL indicated that the optimal temperature and pH values were 40 °C and 9.0, respectively, and the half-lives (t 1/2 ) at 30 °C were 6.52 h. To further improve its catalytic performance, protein engineering of Mt-TPL was undertaken using a consensus sequence-based strategy. It was found that the I384V mutant exhibited the increase of 40.31 % in the enzymatic activity as compared with the wild-type enzyme. Molecular docking and molecular dynamics (MD) simulations revealed that the I384V mutation changed the local flexibility of enzyme and the orientation of substrate, and shortened the proton transfer distance between the generic acid Y71 and substrate pyrocatechol. Finally, the whole-cell catalysts with wild type and I384V Mt-TPL were used to product the L-DOPA, the L-DOPA production of 95.46 g/L for whole-cells with I384V Mt-TPL was achieved in a 5 L fermenter by the fed-batch, 38.6 % higher than that of the wild type enzyme.
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产品关联
DOI
PubMed
高影响因子
Advanced Materials Technologies
·
2026
·
IF 6
Zhiyang Chen,Yu Cai,Shanshan Zhang,Yue Zhou,Yiming Wei,Junhao Li,Rong Xu,Yunbo Gai,Xuesong Ye,Congcong Zhou,Bo Liang
Levodopa (L-Dopa) pharmacokinetic monitoring is crucial for optimizing therapy in Parkinson's disease (PD); however, existing sweat sensors are still limited by their reliance on active perspiration and poor specificity in complex biofluids. Here, we present a passive osmotic differential electrochemical (PODE) patch for continuous, non-invasive L-Dopa monitoring under resting conditions. The patch employs an ethylene glycol-loaded polyacrylamide/polyvinyl alcohol (PAAm/PVA) hydrogel as a “chemical osmotic pump” to drive sweat transport without external power or physical exertion. For sensitive detection, a three-dimensional paper-based electrode is constructed via in situ growth of acrylamide-doped poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and gold nanoparticles (AuNPs). To eliminate interferences from uric acid (UA) and ascorbic acid (AA), a tyrosinase-enabled dual-channel differential sensing strategy is employed. The sensor exhibits a wide linear range and maintains high selectivity in complex artificial sweat. By seamlessly integrating passive sampling with interference-rejecting electrochemical detection, this flexible, low-cost patch offers a practical and user-friendly platform for personalized, at-home management of PD.
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DOI
高影响因子
Journal of Water Process Engineering
·
2026
·
IF 6.7
Bang Li,Juncai Ren,Jiayi Xie,Junfeng Wu,Yingchun Wang,Xiaogang Gu,Xiaoxian Hu,Xiang Guo,Zuwen Liu,Yanbin Yun,Chunli Li,Xiying Hao,Shasha Chen,Shiqiang Yin
The discharge of oily wastewater and industrial wastewater poses serious threats to aquatic ecosystems and human health, highlighting the urgent need for advanced antifouling membranes capable of efficient oil-in-water emulsion separation. Unlike conventional surface coating or single-component modification strategies, herein, we report a facile strategy to transform hydrophobic PVDF membranes into superhydrophilic ones with hierarchically gradient pores. This approach distinctively exploits mussel-inspired L-3,4-dihydroxyphenylalanine (L-DOPA), rather than dopamine, in combination with polyethyleneimine (PEI) co-deposition and carbon nanotubes (CNTs) nanostructuring, forming a highly stable, multifunctional hydrophilic network that anchors CNTs while synergistically regulating pore size, pore distribution, and surface wettability. As a result, the average pore size decreased from 0.4075 μm to 0.2883 μm, enhancing selective permeability. The optimized L-DOPA/PEI/CNTs/PVDF membrane exhibited an ultrahigh pure water flux of 5367.9 L m −2 h −1 bar −1 and achieved over 99 % rejection of diverse surfactant-stabilized emulsions. Moreover, the membrane demonstrated excellent antifouling, consistently maintaining ≥98 % oil rejection over seven cycles, attributed to the formation of a compact hydration layer. By integrating bio-inspired adhesion chemistry with nanostructured pore-gradient engineering，this study provides a scalable and environmentally relevant route for fabricating high-performance separation membranes, offering strong potential for sustainable oily wastewater treatment and resource recovery applications.
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产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Mengzhen Dai,Ting Huang,Long Chao,Qingji Xie,Yueming Tan,Chao Chen,Wenhua Meng
Horseradish peroxidase (HRP)-catalyzed polymerization of l -DOPA (vs. dopamine) in the presence of H 2 O 2 (and uricase (UOx)) was exploited to immobilize mono-/bi-enzymes for hydroquinone-mediated amperometric biosensing of H 2 O 2 and uric acid (UA). The relevant polymeric biocomposites (PBCs) were prepared in phosphate buffer solution containing HRP and l -DOPA (or plus UOx) after adding H 2 O 2 . The mono-/bi-enzyme amperometric biosensors were prepared simply by casting some of the PBCs on Au-plated Au (Au plate /Au) electrodes, followed by coating with an outer-layer chitosan (CS) film for each. UV–vis spectrophotometry , scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used for film characterization and/or process monitoring. The HRP immobilized by enzyme catalysis well preserved its bioactivity, as confirmed by UV–vis spectrophotometry . Under optimized conditions, the monoenzyme CS/HRP-poly( l -DOPA) (PD)/Au plate /Au electrode potentiostated at −0.1 V responded linearly to H 2 O 2 concentration from 0.001 to 1.25 mM with a sensitivity of 700 μA mM −1 cm −2 and a limit of detection (LOD) of 0.1 μM, and the bienzyme CS/UOx–HRP–PD/Au plate /Au electrode at −0.1 V responded linearly to UA concentration from 0.001 to 0.4 mM with a sensitivity of 349 μA mM −1 cm −2 and a LOD of 0.1 μM. The mono-/bi-enzyme biosensors based on biosynthesized PD performed better than many reported analogues and those based on similarly biosynthesized polydopamine.
研究领域:
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 21
·17016-17022
·
IF 3.289
Mengzhen Dai,Ting Huang,Long Chao,Yueming Tan,Chao Chen,Wenhua Meng,Qingji Xie
Learning from nature emerges as one of the most promising ways to develop advanced functional materials and biodevices. Here, inspired by melanin formation, we report the tyrosinase (Tyr)-catalyzed polymerization of L-DOPA (versus L-tyrosine and dopamine) to immobilize enzymes for amperometric biosensing. The enzymatic polymerization is examined by UV-vis spectrophotometry, scanning electron microscopy and electrochemical methods. A poly(L-DOPA) (PD)-Tyr/glassy carbon electrode (GCE) prepared by casting an aqueous mixture of L-DOPA and Tyr on a GCE exhibits a linear cathodic amperometric response to catechol concentration from 0.4 to 57 μM (R2 = 0.997) with a sensitivity of 4.29 mA mM−1 cm−2 and a limit of detection (LOD) of 70 nM (S/N = 3). A PD-glucose oxidase (GOx)-Tyr/Pt electrode prepared by casting an aqueous mixture of L-DOPA, GOx and Tyr on a Pt electrode exhibits a linear anodic amperometric response to glucose concentration from 2 to 5700 μM (R2 = 0.998) with a sensitivity of 78.6 μA mM−1 cm−2 and a LOD of 0.1 μM (S/N = 3). The PD-based enzyme electrode shows better biosensing performance and higher bioactivity of the immobilized Tyr than those based on similarly biosynthesized poly(L-tyrosine) and polydopamine as well as well-established chitosan and Nafion systems, implying that the biosynthesized PD as a melanin-biomimetic material has promising application potential for biomacromolecular immobilization and biosensing.
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DOI
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Kunyin Jiang,Hong Yu,Lingfeng Kong,Shikai Liu,Shaojun Du,Qi Li
The visually appealing shell colours and intricate patterns of shellfish not only attract consumers but also enhance their economic value. Understanding the inheritance pattern of shell colour and the molecular mechanism of pigmentation is highly important for enhancing breeding efficiency. This study involved the identification of a tyrosine hydroxylase gene ( CgTH ) and the investigation of its function in the metabolism of tyrosine and pigmentation in Pacific oysters. CgTH contains a conserved Biopterin_H domain that consists of a binding site for iron atoms and a binding site for tetrahydrobiopterin (BH4). Quantitative PCR (qPCR) and immunofluorescence analysis revealed that the expression of CgTH was higher in the mantle of black shell oysters in comparison to the white strain. The in vitro and in vivo experiments confirmed that Cg TH is involved in tyrosine metabolism through the catalysis of the conversion of L-tyrosine to L-DOPA. In order to examine its functions in melanin synthesis in oysters, 3-Iodo-L-tyrosine (3-IT) treatment, RNA interference (RNAi), and L-DOPA treatment assays were conducted. The results of our study showed that 3-IT effectively inhibited Cg TH activity, leading to a suppression of tyrosine metabolism. Knockdown of Cg TH hindered the cAMP-CREM-MITF axis and decreased pigmentation. On the other hand, L-DOPA stimulated melanin production through cAMP signaling and counteracted the inhibitory effects of 3-IT on the metabolism of tyrosine. These data indicated that there may be a feedback loop involving Cg TH and the cAMP-CREM-MITF axis that regulates tyrosine metabolism and melanogenesis in Pacific oysters. This study enhances our understanding of the molecular mechanism that governs oyster pigmentation and offers a possible molecular indicator for the targeted breeding of particular strains of Pacific oysters ( Crassostrea gigas ).
研究领域:
海洋生物学
·
分子生物学
·
遗传学
·
水产养殖
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DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2014
·
IF 4.787
Yanping Li,Yangyang Zhou,Bowen Qi,Tao Gong,Xun Sun,Yao Fu,Zhirong Zhang
Parkinson’s disease (PD) has become one of the most deadly diseases due to a lack of effective treatment. Herein, N-3,4-bis(pivaloyloxy)dopamine-3-(dimethylamino)propanamide (PDDP), a brain-specific derivative of dopamine, was designed and synthesized, which consists of a brain targeted ligand, N,N-dimethyl amino group, and two dipivaloyloxy groups for lipophilic modification. PDDP was investigated both in vitro and in vivo by comparing with L-DOPA and another derivative (BPD) without N,N-dimethyl amino group. PDDP showed a more pronounced accumulation in mouse brain microvascular endothelial cells (bEnd.3) than BPD via an active transport process. The increased cellular uptake of PDDP was proven to be mediated by putative pyrilamine cationic transporters. Following intravenous administration, the concentration of PDDP in the brain was 269.28-fold and 6.41-fold higher than that of L-DOPA and BPD at 5 min, respectively. Additionally, PDDP effectively attenuated the striatum lesion caused by 6-hydroxydopamine (6-OHDA) in rats. More importantly, PDDP presented antioxidant and antiapoptotic effects on 6-OHDA-induced toxicity in human neuroblastoma cells (SH-SY5Y). Thus, N,N-dimethyl amino group-based PDDP represents an effective and safe treatment for PD.
研究领域:
神经科学
·
药理学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）