CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Chunxu Yu,Baiyang Lu,Yidan Tang,Weilan Zhuang,Xin Zhao,Lulu Guo,Fanzheng Meng,Bingling Li
Timely and accurate detection of a pathogen and its drug-resistance mutations is crucial for guiding clinical treatment and reducing therapy duration. Traditional methods such as PCR, NGS, and microbial culture, though commonly used, have limitations like slow processing, high costs, and limited sensitivity, making them less suitable for urgent clinical needs. Here we propose a dual-position nucleic acid coding strategy, termed Iso-D-nCir, which can achieve one-pot detection of both conserved region and drug-resistant mutations of a pathogen rapidly and precisely. This approach integrates loop-mediated isothermal amplification (LAMP) and catalytic hairpin assembly (CHA) as the isothermal amplification (Iso) and circuit (Cir) to form the LAMP-D-nCir platform as a model, which is low cost, flexibility of use and short detection time. Using Mycoplasma pneumoniae (MP) and its drug-resistant mutations (A2063G and A2064G) as model targets, LAMP-D-nCir demonstrates ultrahigh sensitivity (200 copies/mL) and specificity. Clinical validation across 126 samples demonstrated 100 % concordance with NGS, PCR, and microbial culture. Additionally, the platform exhibits high-resolution discrimination of low-frequency mutations, 1 % mutation abundance from the mixtures can be detected. Furthermore, CHA enables transduction of analyte into diverse signal outputs, such as electrochemical and colorimetric readouts, thereby rendering Iso-D-nCir particularly well-suited for on-site detection in resource-limited environments.
研究领域:
分子诊断
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医学检验
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生物技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
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IF 9.5
Zhongjie Fei,Ping Liu,Chu Cheng,Rongbin Wei,Pengfeng Xiao,Youfa Zhang
Although numerous approaches were proposed for the nucleic acid (NA)-based SARS-CoV-2 detection, the nonideal NA desorption efficiency of conventional magnetic beads (MBs) limits their widespread application. In this study, we developed solvent-responsive MBs (called responsive MBs), which, in the presence of buffers, modulated the absorption and desorption capacities of NA by flipping the surface −COO–. Relative to other commercial MBs, responsive MBs exhibited similar absorption profiles and markedly enhanced desorption profiles. When applied for NA detection of complex samples, responsive MBs exhibited better performance of RNA detection than DNA, with obvious advantages in sensitivity. Specifically, the RNA and DNA desorption rates of commercial MBs were ∼85 and 82.5%, while those of responsive MBs were nearly 94 and 93.5%, respectively. Furthermore, responsive MBs exhibited remarkable extraction ability in a wide range of tissues and better performance of RNA extraction than DNA. When applied for SARS-CoV-2 detection, the responsive MBs along with the simulated digital RT-LAMP (a previously established apparatus) further improved detection efficiency, yielding a precise quantitative detection as low as 25 copies and an ultimate sensibility detection of 5 copies/mL. It was also successfully employed in numerous NA-based technologies such as polymerase chain reaction (PCR), sequencing, and so on.
研究领域:
生物技术
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分子生物学
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病毒学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Long Ma,Jiajing Wang,Yaru Li,Dan Liao,Wenlu Zhang,Xiao Han,Shuli Man
Rapid, sensitive and specific detection of bacteria is of great importance. Herein, we developed a versatile biosensing platform for ultrasensitive detection of pathogenic bacteria, termed as SCENT-Cas (Silver nanoCluster Empowered Nucleic acids Test using CRISPR/Cas12a). Simply, the species-specific invA gene of Salmonella typhimurium (S. typhi) was isothermally amplified using LAMP, which subsequently triggered the trans -cleavage of CRISPR/Cas12a. The trans -cleavage degraded any single-stranded DNA (ssDNA) non-specifically. A DNA-templated AgNCs probe was then employed, in which green fluorescence emissive AgNCs effectively converted to red fluorescence emissive AgNCs when placed in close vicinity to a pre-designed converter ssDNA. As such, the trans -cleavage was utilized for shredding converter ssDNA, enabling the green-to-red fluorescent change to form a ratiometric biosensing platform. With this strategy, target nucleic acid was dexterously converted into ratiometric fluorescence that was recorded to detect as low as 1 CFU/mL S. typhi with a dynamic range from 1 to 10 8 CFU/mL. To our knowledge, this is the first report regarding the use of ratiometric fluorescence in CRISPR/Cas-based detection, which minimizes interference and improves reliability. Lastly, this proposed strategy was challenged by detecting S. typhi contamination in real food samples. Our work enriches CRISPR/Cas toolbox in biosensing by providing a desirable method for bacterial detection.
研究领域:
生物传感器
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分子生物学
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纳米技术
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食品安全
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2018
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IF 6.735
Qinglong Zeng,Xiaofeng Xie,Xiao Wang,Yan Wang,Guanhong Lu,David Y.H. Pui,Jing Sun
The ever increasing problem of air pollution has provoked the research and development of highly-efficient photocatalysts. Herein, Ag nanowires@TiO 2 composite photocatalyst with improved photocatalytic performance was fabricated by a facile one-step solvothermal procedure. The formation of one-dimensional Ag nanowires@TiO 2 core-shell nanostructures could not only broaden the light-absorbing range of TiO 2 catalysts through the surface plasmon resonance effect of Ag nanowires , but also enable the effective separation of photoinduced electron-hole pairs. Under the irradiation of a 260 W fluorescent lamp, the composite with 0.5 wt% Ag nanowires exhibited the highest photocatalytic activity in short contact time (4.8 min), and the corresponding gaseous acetaldehyde removal ratio was 72%, which was much higher than that of bare TiO 2 (37%). The photocatalyst also showed ultrastable activity in the 15 weeks usage, which ensured their practical applications in the air purification field. An in-depth mechanism of the photodecomposition of acetaldehyde was proposed on the basis of the electron spin resonance (ESR) tests and the scavenger experiments. O 2 − reactive radicals was found to play a predominant role in the oxidation and decomposition of acetaldehyde. The outstanding performance of the composite materials and the thoroughly understanding of the reaction mechanism would cast light on the purposively design and optimization of TiO 2 -based catalysts.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
TALANTA
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2022
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IF 6.556
Yaqin Zhang,Xiangyu Quan,Yingchun Li,Hangyu Guo,Fange Kong,Jiahui Lu,Lirong Teng,Jiasi Wang,Di Wang
The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) proteins-based platforms have been used for the detection of pathogens. However, in further applications and research, due to multiple steps needed, many methods showed an increased risk of cross-reactivity. The thermostable Cas12b enables the combination of isothermal amplification and CRISPR-mediated detection, which could decrease the risk of cross-contamination. In this study, we developed a portable and specific diagnostic method that combined the gold nanoparticle (AuNP) with thermal stable CRISPR/Cas12b-enhanced reverse transcription loop-mediated isothermal amplification (RT-LAMP), which is called SCAN, to distinguish the N gene of SARS-CoV-2 from flu gene. We validated our method using RNA from cells transfected by plasmids. We could easily distinguish the positive results by the naked eye based on the strong molar absorption coefficient of AuNP. Moreover, SCAN has the potential for high-throughput tests owing to its convenient operation. In sum, SCAN has broken the site and equipment restrictions of traditional detection methods and could be applied outside of hospitals and clinical laboratories, greatly expanding the test of COVID-19.
研究领域:
分子生物学
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生物技术
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病毒学
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诊断医学
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
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IF 5.195
Shiqi Zhu,Haimei Wen,Wenling Wang,Yong Chen,Fengmei Han,Wentao Cai
Ethnopharmacological relevance Salvia miltiorrhiza (the roots of S. miltiorrhiza Bunge, Danshen in Chinese), a traditional Chinese medicine , has been clinically used to prevent and treat various diseases, such as cardiovascular and cerebrovascular diseases , diabetes, and hepatitis B, in China and some other Asian countries. Lithospermic acid (LA), a polyphenol derived from S . miltiorrhiza , has been reported to exhibit multiple pharmacological properties, such as anti-inflammatory, anti-HIV, and anti-carbon tetrachloride-induced liver injury activities. However, little is known about the anti-hepatitis B virus (HBV) activity of LA. Aim of the study The study was projected to investigate the anti-HBV activity of LA in vitro (HepG2.2.15 and pHBV1.3-transfected HepG2 cells) and in vivo (pAAV-HBV1.2 hydrodynamic injection [HBV-HDI] mice) and explore the potential mechanism as well. Materials and methods Hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) contents were detected by ELISA kits. HBV DNA and hepatitis B core antigen (HBcAg) levels were evaluated by quantitative real-time polymerase chain reaction and immunohistochemistry assay, respectively. The proteins in autophagy process, lysosomal acidic function, and autophagy-related signaling pathways were examined by Western blot . Transmission electron microscopy was used to observe the number of autophagosomes and autolysosomes. Confocal microscopy was applied to analyze the autophagic flux and lysosomal acidification, using mCherry-enhanced green fluorescent protein (EGFP)-microtubule-associated protein light chain (LC)3 and lysosomal probes, respectively. Results LA exhibited anti-HBV activity by inhibiting HBV DNA replication in HepG2.2.15 and pHBV-transfected HepG2 cells in dose- and time-dependent manners and hampering HBsAg and HBeAg levels in HepG2.2.15 cells to a certain extent. LA reduced HBV DNA, HBsAg/HBeAg, and HBcAg levels in the serum/liver tissues of HBV-HDI C57BL/6 mice during the 3-week treatment and suppressed the withdrawal rebound of HBV DNA and HBsAg in the mice serum. LA increased LC3-II protein expression and the number of autolysosomes/autophagosomes and promoted the degradation of sequestosome 1(p62) protein in vitro and in vivo . LA enhanced the co-localization of LC3 protein with autolysosomes, further confirming the ability of LA to induce a complete autophagy. Knockdown of autophagy-related gene (Atg) 7 or 5 in vitro and administration of 3-methyladenine (an autophagic inhibitor) in vivo disabled the inhibitory efficacy of LA on HBV DNA replication, suggesting that the anti-HBV efficacy of LA depended on its ability of inducing autophagy. LA could enhance lysosomal acidification and improve the function of lysosomes by promoting the protein expression of lysosomal-associated membrane protein (LAMP)-1, LAMP-2, and mature cathepsin D , which may contribute to the autophagic induction of LA. LA inhibited the activation of AKT and mammalian target of rapamycin (mTOR) induced by HBV, which was reversed by IGF-1 (an agonist of the PI3K/AKT/mTOR signaling pathway), indicating that LA elicited autophagy through hampering the PI3K/AKT/mTOR signaling pathway. Conclusion We revealed the anti-HBV activity and mechanism of LA in vitro and in vivo . This study facilitates a new understanding of the anti-HBV potent components of S. miltiorrhiza and sheds light on LA for further development as an active constituent or candidate used in the therapy against HBV infection.
研究领域:
药理学
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病毒学
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分子生物学
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中医药
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产品关联
DOI
PubMed
高影响因子
CATALYSIS TODAY
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2024
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IF 5.2
Wangjun Shi,Min Liu,Haodong Sun,Jiang Yu
In this study, a microwave discharge electrodeless lamp (MDEL) photo-degradation system coupled with anatase TiO 2 (P25) was constructed to improve the selective degradation of NH 3 to N 2 . The MDEL system can generate abundant reactive oxygen species (ROS) with ultraviolet radiation at 185 nm and 254 nm during gas phase reaction . The species and content of ROS generated in the reaction system were determined by oxidation extraction spectrophotometry , and the effects of residence time, temperature, and relative humidity on the production of reactive oxygen species were investigated. In addition, the removal efficiency under different conditions such as initial ammonia concentration, relative humidity and residence time was explored. It was found that the removal rate could reach 83% when the initial ammonia concentration was 100 ppm, relative humidity was 55% and gas residence time was 5.16 s. The high-energy ultraviolet at 185 nm from MDEL could break NH 3 molecules in advance and produced a large amount of ·NH 2 . Besides, NH 3 was converted to N 2 by generating large amounts of ROS, reducing NO x and nitrate generation. Our work presents a novel approach to construct a photocatalytic system for high efficiency degradation of NH 3 .
研究领域:
环境科学
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化学工程
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
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IF 2.931
Hongwei Hu,Xinmeng Tian,Yixuan Gong,Guolan Ren,Jiangong Liang
Despite extensive research on carbon dots (CDs), rare studies have been performed on the photostability of CDs. Here, the photostability of CDs synthesized with 3-aminobenzoic acid were systematically investigated under different pH conditions (5.0, 7.4 and 9.0). The results showed that under Xenon lamp irradiation, the fluorescence (FL) intensity of the CDs exhibited an increase first and then a decrease, with a gradual shift of the maximum emission wavelength to longer wavelength. Further investigation indicated that the irradiation induced the change of the CD surface functional groups and gave rise to aggregation, resulting in the formation of larger particles. This study provided important reference value towards research on CDs properties.
研究领域:
材料科学
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光学
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化学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Hong-Yu Ren,Ying-Qi Dai,Fanying Kong,Defeng Xing,Lei Zhao,Nan-Qi Ren,Jun Ma,Bing-Feng Liu
In this study, the growth and lipid accumulation of Scenedesmus sp. using different nanoparticles and light sources were investigated. Xenon lamp can produce a broad illumination spectrum, and exhibited better performance than light-emitting diode. SiC and g-C 3 N 4 nanoparticles improved the biomass and lipid accumulation, whereas TiO 2 and TiC nanoparticles had inhibitory influence on microalgae. Lipid production can be improved by oxidative stress produced by combination of nanoparticles and xenon lamp irradiation. At the optimal SiC nanoparticles concentration of 150 mg L −1 and photoperiod of 6:18 h, the maximum biomass concentration and total lipid content reached 3.18 g L −1 and 40.26%, respectively. The addition of SiC nanoparticles could promote the substrate utilization rate and induce stress condition, thereby enhancing the activity of acetyl-CoA carboxylase and lipid biosynthesis. This research shows that SiC nanoparticles addition combined with xenon lamp illumination is a promising strategy to promote microalgal growth and lipid accumulation.
研究领域:
生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Public Health
·
2026
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IF 4.1
Qizhi Diao,Sha Tang,Xin Liu,Jiajia Li,Xiangmin Zhou,Yuanyu Chen,Qiongyuan Zhang,Fangyu Yang
Background This study aimed to establish a rapid detection method for SARS-CoV-2 by integrating loop-mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) technology. Method Specific primers targeting the nucleocapsid (N) gene and open reading frame 1ab (ORF1ab) gene of SARS-CoV-2 were designed and labeled with 6-FAM and biotin, respectively. After systematically optimizing key reaction parameters, including primer selection, primer concentration, and LAMP reaction time, the sensitivity, specificity, and clinical applicability of the method were evaluated. Results The proposed LAMP-LFD assay enables rapid detection within 30 min with high specificity, accurately identifying the N and ORF1ab genes of SARS-CoV-2 without cross-reactivity with influenza A hemagglutinin gene, influenza B neuraminidase gene, and respiratory syncytial virus M gene. The limit of detection reached 1.892 × 10 1 copies/μL, showing comparable sensitivity to agarose gel electrophoresis and real-time quantitative reverse transcription PCR (RT-qPCR). The results were consistent across different batches of primers and probes, demonstrating good reproducibility. When compared with RT-qPCR using 114 inactivated throat swab samples, the diagnostic agreement rate reached 95.61%. Discussion This study provides technical support for the surveillance and control of SARS-CoV-2 infection.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2017
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IF 6.065
Lingjun Meng,Shaogui Yang,Cheng Sun,Huan He,Qiming Xian,Shiyin Li,Guoxiang Wang,Limin Zhang,Dong Jiang
In this study, an electromagnetic induction electrodeless lamp (EIEL) was first introduced into UV advanced oxidation processes (AOPs) for photodegradation of Diatrizoate (DTZ), which was the most persistent iodinated X-ray contrast medium (ICM), and traditional Hg lamps were taken as references. Direct photolysis rate of DTZ under EIEL irradiation was 1.34 times as that under Hg irradiation, but the electric energy consumption was 0.87 times. In this sense, the combination of EIEL and oxidants (O 2 , H 2 O 2 and S 2 O 8 2− (PS)) was further investigated. The remarkably increased photodegradation rates were observed in UV/PS system due to primary contribution rate of SO 4 − (62.5%) based on the results of radical concentrations and second-order rate constants of DTZ with SO 4 − and OH. Inorganic ions influencing the photodegradation process were investigated. The effect of natural organic materials (NOMs) in UV/PS system was studied based on contribution ratios of light screening effect and quenching. Transformation mechanisms of DTZ in UV/PS system included deiodination, intramolecular cyclization, decarboxylation, deacetylation and deamination, which were further confirmed by frontier electron density calculations. The study indicated that UV/PS with EIEL irradiation has the potential to remove pharmaceuticals in contaminated aquatic environments.
研究领域:
环境科学
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化学工程
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水处理技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2019
·Vol. 103
·期 3
·1798-1808
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IF 3.094
Nan Zhou,Longhai Liu,Zhipeng Zhou,Ye Zhang,Minghui Li,Cheng Zhou,Mao Xia,Zhi Zhou
In the recent years, Mn 4+ -doped phosphors for indoor plant cultivation have received extensive concern owing to the far-red emission that can match well with the absorption spectra of plant pigments. Whereas, many Mn 4+ -doped phosphors still face some challenges such as poor light efficiency and low thermal stability. It is an effective way to resolve these problems via cation vacancies engineering. Herein, the Ca 14− x Al 10 Zn 6− y O 35 : Mn 4+ phosphors are successfully synthesized by combustion method. The luminescence intensity of Ca 14− x Al 10 Zn 6− y O 35 : Mn 4+ phosphor is enhanced through engineering Ca 2+ and Zn 2+ vacancies according to the charge compensation mechanism. The optimal content of each Ca 2+ and Zn 2+ vacancy is equal to be 0.3. Furthermore, the defect formation is accompanied with lattice distortion, which plays a vital role in driving the excited phonon traps to reduce the energy loss by non-radiation transitions. Therefore, the thermal stability of Ca 14− x Al 10 Zn 6− y O 35 : Mn 4+ phosphor is also improved via engineering cation vacancies. In addition, the Ca 14− x Al 10 Zn 6− y O 35 : Mn 4+ phosphors can be effectively excited by blue light and it exhibits far-red emission due to the Mn 4+ spin-forbidden 2 E → 4 A 2 transition. The results suggest that the Ca 14− x Al 10 Zn 6− y O 35 : Mn 4+ phosphors can have a tremendous potential in indoor plant cultivation.
研究领域:
材料科学
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光学材料
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园艺照明
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固态化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）