ANALYTICAL LETTERS
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2023
·
IF 2
Qing Zhang,Chunrong Zhang,Nan Li,Zhao Dai
Aptamer-based biosensors have been demonstrated to provide real-time physical and chemical signals for biomedicine, food safety, drug identification, and metal ion determination. In this study, cadmium ion (Cd2+) was determined in drinking water by color signaling using the specific binding of the aptamer and target by its regulation of the catalytic capacity of gold nanoparticles (AuNPs). When sodium borohydride (NaBH4) and a probe with a catalytic function were present, the solution containing 4-nitrophenol changed from yellow to colorless. Under optimal conditions, the naked eye was sufficient to determine Cd2+ with a limit of detection (LOD) of 5 μM. The discoloration time showed a positive correlation with Cd2+ concentration up to 30 μM. This method was label-free, efficient, rapid, and selective for Cd2+ in water without expensive instruments, suggesting practical applications.
研究领域:
分析化学
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环境科学
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纳米技术
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生物医学工程
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Jin Birui,Ma Chuan,Zhang Chuyao,Yin Huiling,Zhao Guoxu,Hu Jie,Li Zedong
There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach. Graphical Abstract
研究领域:
病毒学
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诊断方法
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纳米技术
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即时检测
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DOI
PubMed
高影响因子
Sensors and Actuators Reports
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2025
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IF 7.6
Junting Liang,Dihan Duan,Lubing Sun,Jinming Li,Mengke Wang,Ziyi Chang,Rick F. Thorne,Chuanliang Chen,Demin Duan
Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn 3 O 4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K m value of 0.1896 mM and a high V max of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn 3 O 4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.
研究领域:
生物化学
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分析化学
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纳米技术
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疾病诊断
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Tao Zhang,Chaoxin Zhang,Yingzhan Chen,Hongjie Liu,Shaopeng Wang,Yaling Liu,Yayi He,Man Zhang,Liwei Wang
Harmful algal blooms (HABs) have posed a significant threat to human society and the ecological environment. In particular, the outbreak of Phaeocystis globosa ( P. globosa ) bloom could affect coasts nuclear power safety. Unfortunately, current ecological monitoring tools fail to dynamically detect the densities of solitary cells from P. globosa in the pre-outbreak phases, thus affecting early interventions. In the study, an effective electrochemical DNA biosensor was developed to serve the rapid and effective detection of P. globosa DNA through a specific DNA probe strategy. Especially, its good specificity and lower limit of detection (LOD, 17 pg/μL or 1063 cells/L) met the monitoring requirement of solitary-cell population change of P. globosa before the cyst formation (threshold: 1.0 × 10 7 cells/L), which is the key step in the algal bloom outbreaks and influences the outbreak cycle and scale. Furthermore, the accuracy of this electrochemical biosensor for the quantitative detection of solitary-cell P. globosa was confirmed by using the classical microscopic examination techniques (r = 0.981, P < 0.001). Moreover, its applicability was also validated by actual sample testing (r = 0.996, P < 0.001). Therefore, the novel technology offers great potential to improve dynamic detection and early warning of P. globosa bloom.
研究领域:
环境科学
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生物技术
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海洋生物学
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分析化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Huifang Wu,Yuwei Ling,Shiying Ju,Yubing Chen,Mengqi Xu,Yanfeng Tang
Phosphate (Pi) plays an essential role in aquatic ecosystems as well as in physiological processes. Here, a dual-emission probe for the sensitive, specific and visual analysis of Pi is fabricated by coordinating Eu 3+ with luminol and 2,6-pyridinedicarboxylic acid (DPA). Pi can significantly enhance the characteristic fluorescence of Eu 3+ at 615 nm by promoting energy transfer from DPA to Eu 3+ and reducing the quenching effect of water molecule, luminol with inherent emission at 423 nm further enhances the Eu 3+ fluorescence. Accordingly, ratiometric detection of Pi can be achieved with the fluorescence ratio F 615 /F 423 as a function of Pi concentration. Linearity between F 615 /F 423 and Pi concentration in the range of 0.1–25 μM is shown, and the limit of detection (LOD, 3σ/K) for Pi is 0.027 µM. In addition, a continuous change in the fluorescence color of the probe from blue to red is observed with increasing Pi concentration under a UV lamp, and a smartphone-based visual method is used for the convenient and effective semi-quantitative determination of Pi. The dual-emission probe has been successfully applied to ratiometric and visual analysis of Pi in human urine and environmental water samples, and adequate results are obtained.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
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2022
·Vol. 53
·期 3
·559-569
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IF 2.925
Li Shun,Niu Airong,Lan Cuixia,Xu Xuanming,Sun Shukai,Xuan Chao,Zhao Peng,Tian Qingwu,Zhou Tingting
A novel electrochemical sensor of multi-walled carbon nanotube (MWCNT) and stearyl trimethyl ammonium chloride (STAC) modified acetylene black paste electrode (AB-PE) was successfully established. An AB/MWCNT/STAC-paste electrode was constructed using the AB electrode with MWCNT and STAC as the electrochemical sensor materials. The performance of the prepared sensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. Some factors, such as sensor composition, adsorption, and detection conditions, which affect the property of the sensor, were investigated in detail. The results demonstrated that the prepared sensor has satisfactory selectivity, repeatability, and stability for methotrexate (MTX) detection. The linear range of the method for MTX detection was 0.02–8.0 μM with a limit of detection (LOD) of 1.81 nM. Finally, the sensor was successfully used for detecting the MTX concentrations in the samples of serum and saliva. The prepared sensor could be a fresh platform for the swift and accurate MTX detection in clinical samples. Graphical abstract
研究领域:
电化学
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纳米技术
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分析化学
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临床化学
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DOI
高影响因子
FOOD CONTROL
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2022
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IF 6.652
Gaozhen Li,Fukai Guan,Shancang Zhao,Huihui Xu,Jiashuai Sun,Jingcheng Huang,Qingqing Yang,Yanyin Guo,Xia Sun,Yemin Guo
As an organochlorine pesticide, procymidone has become an increasing serious problem in vegetables in recent years. In order to solve a series of problems such as low sensitivity of the immunological methods, a time-resolved fluorescent immunoassay strip (TRFIAS) was developed with low limit of detection (LOD). Based on the principle of antigen-antibody immune competition, TRFIAS could detect procymidone in vegetables. After the targets being added, the samples were incubated at 37°C for 10 min. TRFIAS was observed under a UV lamp of 365 nm. The results were processed by ImageJ software to complete the qualitative and quantitative analysis of TRFIAS. Under the optimal working conditions, a linear range of TRFIAS was 5–250 ng/mL with a LOD of 0.33 ng/mL and a visual limit of detection (VDL) of 50 ng/mL. And the TRFIAS had good specificity, precision and accuracy. Comparing the results of TRFIAS with those of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), the two assays were in good agreement. Therefore, TRFIAS has the advantages of high sensitivity, high specificity, simple operation and relatively short assay time. Meanwhile, it shows absolutely great advantages in both quantitative and qualitative analysis.
研究领域:
农业化学
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分析化学
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食品科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Bin Qiao,Jiakun Xu,Wenhao Yin,Wanmeng Xin,Lixin Ma,Jie Qiao,Yi Liu
Herein, we report a method that combined “aptamer-locker” DNA with CRISPR/Cas12a-based biosensing for sensitive and rapid melamine analysis. Three strategies were harnessed for designing the DNA sensors that were well characterized by circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC) in the absence and presence of melamine. The detection parameters were optimized to achieve good analytic performance. As a result, a limit of detection (LOD) as low as 38 nM was achieved, which is below the threshold (1.0 mg/kg) of allowable melamine in infant milk products. In addition, the sensors show high selectivity for melamine against other analogues such as cyanuric acid , ammeline and ammelide . Moreover, our method was effective for rapid melamine analysis in whole milk samples, with or without sample pretreatment , in less than 20 min. Adopting a commercially available portable fluorimeter, on-site analysis of melamine in milk was accomplished. The strategies demonstrated here can expand to detect other non-nucleic-acid targets by simply replacing the aptamers.
研究领域:
分析化学
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分子生物学
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生物技术
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食品安全
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DOI
PubMed
高影响因子
TALANTA
·
2019
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IF 4.916
Yuehai Li,Jiabai Cai,Fengjiao Liu,Hui Yang,Ye Lin,Shunxing Li,Xuguang Huang,Luxiu Lin
Using chloroauric acid as precursor and β-cyclodextrin (β-CD) as reducing agent and stabilizer, β-CD@AuNPs with negative charge were synthesized by one-step colloidal synthesis method. The positive charged carbon quantum dots (CQDs) were synthesized by one-step of sonication of cetylpyridinium chloride . Under the role of static electricity, the fluorescence resonance energy transfer (FRET) occurred between CQDs and β-CD@AuNPs. CQDs and β-CD@AuNPs served as the fluorescence energy donors and receptors, respectively, i.e., the fluorescence of CQDs was turned off by β-CD@AuNPs. Based on the specific host-guest recognition between the inner cavity of β-CD and cholesterol, CQDs was replaced by cholesterol, the FRET could be interrupted, and then the fluorescence of CQDs was turned on. A good linear relationship between cholesterol concentration (10–210 μmol L −1 ) and fluorescence intensity was obtained and the LOD was 343.48 nmol L −1 . Because of excellent fluorescence quenching ability of FRET, the analytical performance (including LOD and linear scope) of such a turn off-on fluorescent nanosensor (e.g., CQDs/β-CD@AuNPs) was better than nanosensor only via competitive host-guest recognition (e.g., β-CD functionalized CQDs). The synergistic effect of competitive host-guest recognition and FRET was proved. Because of selective recognition, ultrasensitive, wide linear range, and strong anti-interference ability, CQDs/β-CD@AuNPs as a turn off-on fluorescent nanosensor was developed to determine cholesterol in porcine serum.
研究领域:
分析化学
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-12
·
IF 5.833
Hong Juncheng,Wang Wenhai,Wang Jiaqi,Wang Xiao,Xie Hongzhen,Li Tianhua,Gan Ning
A pair of composite probes based on aptamer modified polyhedral oligomeric silsesquioxane-perovskite quantum dots (POSS-PQDs-Apt) as signal probe and titanium carbide (Ti 3 C 2 ) MXenes as quencher were prepared for the first time. They were employed to fabricate one turn-on–type aptasensor relying on fluorescence resonance energy transfer (FRET) for Vibrio parahaemolyticus (VP) determination. The POSS-PQDs-Apt can be adsorbed on the MXenes nanosheets, and its fluorescence was quenched due to the FRET. After the composite probes were incubated with VP for 50 min, the POSS-PQDs-Apt binding with VP can be released from the surface of MXenes, and the signal recovered due to its higher affinity to the VP than MXenes. The fluorescence intensity from 519 nm emission of the system was measured at 480 nm excitation. Under In optimized conditions, the assay can determine VP in the concentration range 10 2 - 10 6 cfu/mL, and the detection limit (LOD) was 30 cfu/mL using fluorescence detection. The LOD is still 100 cfu/mL by naked eye detection which is proper for on-line monitoring VP in aquaculture water. This method was also used to detect VP in actual samples of seawater, the recovery of spiked samples was between 93% and 106%, and relative standard deviation (RSD) was between 2.7% and 6.7%. The result is consistent with the plate count. Therefore, this assay could provide a candidate platform for screening VP in aquaculture industry. Graphical abstract
研究领域:
生物传感器
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分析化学
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环境监测
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Meiling Tian,Wenyue Xie,Ting Zhang,Yang Liu,Zhisong Lu,Chang Ming Li,Yingshuai Liu
This work reports a low-cost, fast and highly sensitive lateral flow immunoassay (LFIA) platform with Prussian blue nanoparticle (PBNP) as a new reporter, which efficiently mediates an on-demand cascade signal amplification by catalyzing oxidation of chromogenic reagent 3,3′,5,5′-tetramethylbenzidine (TMB). In this case, PBNPs are accumulated on the test line through the specific immune interaction, resulting in a blue-colored line. The colorimetric signal can be further amplified via the peroxidase mimic PBNP-catalyzed TMB oxidation to produce an insoluble blue-violet precipitate. Under an optimal condition, a limit of detection (LOD) at 10 pg mL −1 is achieved for rabbit IgG (RIgG) detection, which is 2–3 orders of magnitude better than the conventional LFIA strip using colloidal gold nanoparticle as a label. A macromolecular RIgG and a small molecular mycotoxin ochratoxin A (OTA) are also successfully analyzed in mimicking samples, demonstrating its good practicability of the proposed platform in a variety of fields.
研究领域:
纳米技术
·
分析化学
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生物传感器
·
免疫学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Menglun Zhang,Jingze Huang,Weiwei Cui,Wei Pang,Hao Zhang,Daihua Zhang,Xuexin Duan
Micro/nano scale biosensors integrated with the local adsorption mask have been demonstrated to have a better limit of detection ( LOD ) and less sample consumptions. However, the molecular diffusions and binding kinetics in such confined droplet have been less studied which limited further development and application of the local adsorption method and imposed restrictions on discovery of new signal amplification strategies. In this work, we studied the kinetic issues via experimental investigations and theoretical analysis on microfabricated biosensors. Mass sensitive film bulk acoustic resonator (FBAR) sensors with hydrophobic Teflon film covering the non-sensing area as the mask were introduced. The fabricated masking sensors were characterized with physical adsorption of bovine serum albumin (BSA) and specific binding of antibody and antigen. Over an order of magnitude improvement on LOD was experimentally monitored. An analytical model was introduced to discuss the target molecule diffusion and binding kinetics in droplet environment, especially the crucial effects of incubation time, which has been less covered in previous local adsorption related literatures. An incubation time accumulated signal amplification effect was theoretically predicted, experimentally monitored and carefully explained. In addition, device optimization was explored based on the analytical model to fully utilize the merits of local adsorption. The discussions on the kinetic issues are believed to have wide implications for other types of micro/nano fabricated biosensors with potentially improved LOD .
研究领域:
生物传感器
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分析化学
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纳米技术
·
生物物理
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DOI
PubMed
高影响因子
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