Food & Function
·
2020
·Vol. 11
·期 5
·3823-3837
·
IF 4.171
Zhijing Liu,Fei Liu,Wan Wang,Changbao Sun,Da Gao,Jiage Ma,Muhammad Altaf Hussain,Cong Xu,Zhanmei Jiang,Juncai Hou
Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD) that has serious harmful effects on human health. Lactobacillus rhamnosus, a probiotic, has a strong colonization and adhesion effect and improves the intestinal health of the host. Inulin has good anti-inflammatory effects and can promote the proliferation of beneficial intestinal bacteria. The purpose of this study was to investigate the alleviating effects of L. rhamnosus 1.0320 in combination with inulin on UC, examining the resulting changes in intestinal flora. A UC model was established by having mice freely drink a 3% (w/v) dextran sodium sulphate (DSS) solution for seven days. After successful modeling, the mice were given antibiotics, L. rhamnosus 1.0320 by itself, inulin by itself, and L. rhamnosus 1.0320 combined with inulin as an intragastric intervention for 28 days. The abundance and structural changes of bacteria in the intestinal content of mice were analyzed by 16S rDNA high-throughput sequencing. The study found that male BALB/c mice can successfully establish a typical model of small intestinal inflammation by freely drinking a 3% DSS solution for one week. L. rhamnosus 1.0320 combined with inulin can alleviate DSS-induced colitis, reduce the Disease Activity Index (DAI) score of the pathological damage of colon tissue, decrease myeloperoxidase (MPO) activity, increase hemoglobin content, and regulate the expression levels of inflammatory cytokines IL-1β, IL-6, TNF-α and IL-10. The intestinal flora of mice is reduced after enteritis, and its structure gets disordered. The combination of L. rhamnosus 1.0320 and inulin can increase the abundance and diversity of intestinal flora, and increase the content of beneficial bacteria. Prebiotics promote the colonization ability of probiotics. L. rhamnosus 1.0320 combined with inulin can change the intestinal flora to relieve ulcerative colitis, providing a new theoretical basis for the study of UC mechanism.
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BIOMEDICINE & PHARMACOTHERAPY
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2016
·
IF 2.326
Yaser Gamallat,Abdo Meyiah,Eugene D. Kuugbee,Ahmed Musa Hago,Gift Chiwala,Annoor Awadasseid,Djibril Bamba,Xin Zhang,Xueqi Shang,Fuwen Luo,Yi Xin
Background/Aim Probiotics have been suggested as prophylactic measure in colon carcinogenesis . This study aimed at determining the potential prophylactic activity of Lactobacillus rhamnosus GG CGMCC 1.2134 (LGG) strain on colorectal carcinogenesis via measuring its effect on Nuclear factor kappa B (NFκB) inflammatory pathway and apoptosis . Materials and methods 64 Sprague Dawley rats were grouped into four as follows; Group 1 (Healthy control), Group 2 (LGG), Group 3 (cancer control Dimethyl hydrazine (DMH)) and Group 4 (LGG + DMH). LGG was administered orally to LGG and LGG + DMH groups. Colon carcinogenesis was chemically induced in LGG + DMH and DMH groups by weekly injection of 40 mg/kg DMH. Animals were sacrificed after 25 weeks of experiment and tumor characteristics assessed. The change in expression of NFκB-p65, COX-2, TNFα, Bcl-2, Bax, iNOS, VEGFα, β-catenin, Casp3 and p53 were evaluated by western blotting and qRT-PCR. Results LGG treatment significantly reduced tumor incidence, multiplicity and volume in LGG + DMH treatment group compared to DMH cancer control group. Also, LGG treatment reduced the expression of β-catenin and the inflammatory proteins NFκB-p65, COX-2 and TNFα; the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic proteins Bax , casp3 and p53 compared with DMH group. Conclusion LGG have a potential protection effect against colon carcinogenesis; inducing apoptosis and ameliorating inflammation, and may hold a promise as bio-therapeutic dietary agent.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Fei-Long Li,Qiang Zhou,Wei Wei,Jian Gao,Ye-Wang Zhang
Enzymatic NADP + regeneration is a promising approach to produce valuable chemicals under economic conditions. Among all the enzymatic routes, using water-forming NADH oxidase is an ideal one because there is no by-product. However, most NADH oxidases have a low specific activity to NADPH. In this work, a thermostable NADH oxidase from Lactobacillus rhamnosus (LrNox) was rationally engineered to switch its specificity from NADH to NADPH. The results show that mutants D177A, G178R, D177A/G178R, D177A/G178R/L179S improved the NADPH activity by a factor of 4–6. The highest NADPH catalytic efficiency ( K cat /K m 223.71 S −1 μm −1 , 47.6-fold higher than wild-type LrNox) and 51% of NADH activity retention were achieved by replacing the single amino acid Leu179 for serine (L179S) in LrNox. Modeling of L179S-NADPH complex reveals that the phosphate group of NADPH interacts with the hydroxyl of Ser179 with a strong hydrogen bond and several shorter hydrogen bonds with the amino group of Lys185 could stabilize the binding of NADPH in the L179S mutant. This work provides an efficient method for converting NAD(P)H specificity and shows that L179S mutant is a potential and efficient auxiliary enzyme for NADP + regeneration.
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Journal of Functional Foods
·
2025
·
IF 4
Zhaoxi Deng,Yanfei Ma,Kangwei Hou,Jinyan Dai,Yifan Zhong,Yingying Mao,Jinzhi Zhang,Haifeng Wang
Quorum sensing (QS) is an essential pathway for bacterial communication through autoinducers (AIs). The luxS gene involved in QS has been proven to regulate different behavior of Lactobacillus rhamnosus GG (LGG), whereas it is unclear whether the AI-2/LuxS system have beneficial effects the host by regulating LGG. Our aim was to investigate whether luxS mutation affects the ability of LGG to maintain intestinal microbiota homeostasis, intestinal barrier integrity and inflammatory immune responses in mice infected with Escherichia coli ( E. coli ). This study showed that both wild-type (WT) and luxS mutant (ΔluxS) of LGG can alleviate changes in the microbiota caused by E. coli by down-regulating the relative abundance of Parasturtella . Supplementation with WT and ΔluxS increased the CD4 + /CD8 + ratio in spleen, improved goblet cell numbers and enhanced Muc 2 secretion. Moreover, WT and ΔluxS decreases proinflammatory mediator levels by inhibiting MAPK and NF-κB signal transduction.
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CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Huizhen Zheng,Meng Gao,Ying Ren,Ruyun Lou,Hongguo Xie,Weiting Yu,Xiudong Liu,Xiaojun Ma
A pH-responsive carrier based on an ethylenediaminetetraacetic-calcium-alginate (EDTA-Ca-Alg) system was developed by controlling the release of Ca 2+ . The system remained in the solution state at neutral pH since EDTA completely chelated the Ca 2+ . In contrast, a hydrogel immediately formed when the pH was below 4.0, which triggered the in situ release of Ca 2+ from the EDTA-Ca compound and led to alginate-Ca binding. Taking advantage of the pH sensitivity, we prepared hydrogel microspheres with uniform size to entrap Lactobacillus rhamnosus ATCC 53103 through emulsification. In an acidic environment, the hydrogel structure remained compact with negligible pores to protect L. rhamnosus ATCC 53103. However, in a neutral intestinal environment, the hydrogel structure gradually disassembled because of the Ca 2+ release from the hydrogel, which caused cell release. Therefore, a pH-responsive carrier was developed for the protection and the controlled release of cells in gastrointestinal tract, thus providing potential for oral delivery of probiotics.
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Food Analytical Methods
·
2022
·Vol. 15
·期 12
·3379-3389
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IF 3.498
Wang Jiamin,Zhou Wenyuan,Gao Lu,Yang Zhenquan,Yang Zhanjun,Chen Dawei,Gao Yajun
In this paper, we have developed a fast and low-cost label-free electrochemical immunosensor for detection of Lactobacillus rhamnosus GG (LGG). The proposed sensor is based on a glassy carbon electrode modified with Cu@Cu 2 O nanoparticles anchored B, N co-doped porous carbon (BNPC) composite. The copper-derived nanoparticles with multiple valence states undergo dramatic changes in surface areas, conductivity, and actives sites due to their combination with BNPC, resulting in enhanced electrochemical performance. In the amperometric immunoassay, the immune complex of antigen and antibody inhibits the transfer of electrons on the electrode interface, causing a change in the current. Thus the quantitative detection of LGG is achieved. Differential pulse voltammetry (DPV) was performed at a LGG concentration of 10 2 to 10 8 colony-forming units CFU mL −1 . The immunosensor showed a good linear relationship between the decrease in peak current and the concentration of LGG ( R 2 = 0.9934), with a short detection time and a detection limit of 12 CFU mL −1 . In short, we have developed a highly sensitive electrochemical immunosensor for the specific detection of LGG using the current-mode, label-free immunoassay method evaluated in this study, providing a reliable mean for quantifying probiotics.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2024
·Vol. 59
·期 4
·2472-2486
·
IF 2.6
Jun Wang,Yue Tong Zhao,Bo Cheng Wei
Summary Recently, there has been growing interest in intentionally incorporating fermentation starters into the traditional brewing process. Our study examined the impact of Saccharomyces cerevisiae and Lactobacillus rhamnosus on the flavour profiles of sweet fermented glutinous rice (SFGR). Saccharomyces cerevisiae obviously increased the ethanol content, whereas Lactobacillus rhamnosus notably raised the total acidity. Lactic acid became the dominant organic acid after the addition of Lactobacillus rhamnosus . The volatiles were analysed using electronic nose (E-nose) and gas chromatography-ion mobility spectrometry (GC-IMS). S4 sensor of E-nose exhibited the most extensive response to all samples. Saccharomyces cerevisiae increased ethanol and 3-methylbutan-1-ol levels, while Lactobacillus rhamnosus led to a significant increase in acetone. Principal component analysis effectively discriminated the aroma of SFGR with different starters. Additionally, a strong correlation was observed between the E-nose and GC-IMS results. Our study shows that Saccharomyces cerevisiae and Lactobacillus rhamnosus can enhance the flavour of fermented rice products.
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ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2023
·
IF 3.2
Lijie Zhang,Chuankai Zhang,Mingming Song,Danqi Dang,Jianan Zhao,Liyang Zhang,Tong Fu
The aim of the present study was to characterize the fermentation properties of Lactobacillus rhamnosus GG (LGG; ATCC 53103) in vitro, and investigate the effects of inoculation with LGG on fermentation quality of whole plant corn silage (WPCS) and fermented total mixed ration (FTMR). The antifungal activity of LGG against 4 prevalent fungi isolated from corn stalk was determined using the Oxford cup method. The binding capacity of LGG to aflatoxin B 1 , zearalenone and deoxynivalenol was examined by in vitro adsorption experiments using a batch mode in phosphate buffered saline. The antioxidant activity of LGG in vitro was evaluated by determining the resistance to hydrogen peroxide, free radical scavenging capacity and reducing activity. Three batches of whole plant corn and total mixed ration were collected separately. Each batch of raw material was divided into equal portions and applied to 2 treatments: (1) control with distiller water (CON), and (2) treatment inoculated with LGG (LGG). After 48 d of ensiling, the chemical composition, ruminal degradability, microbial community and mycotoxin concentrations of WPCS and FTMR were determined. The results showed that lactic acid was the predominant component among organic acids in LGG culture process, with a rapid generated occurring at 4 h after inoculation and reaching a stable state at approximately 48 h. Lactobacillus rhamnosus GG could effectively inhibit the growth of 4 prevalent fungi isolated from corn stalk, the diameter of inhibition zone was higher for Mucor circinelloides than that of the other moulds ( P = 0.016). With increasing of LGG cell amount, the binding capacity to 3 mycotoxins increased ( P < 0.01); the heat and acid treated LGG also enhanced the binding capacity to mycotoxins ( P < 0.01). Lactobacillus rhamnosus GG displayed tolerance to hydrogen peroxide (0.8 mmol/L) for 8 h at 37 °C (96%, viability), but the viability decreased sharply (52.39%, viability) under the concentration of H 2 O 2 increased to 1.2 mmol/L ( P < 0.001). The free radical scavenging activity and reducing activity of viable LGG were greatest, followed by cell-free extracts and inactivated cells ( P < 0.001). Ensiling of WPCS and FTMR inoculated with LGG decreased dry matter loss ( P < 0.05), increased lactic acid and acetic acid production ( P < 0.05), enhanced some nutrients ruminal degradability ( P < 0.05). Meanwhile, the inoculation of LGG reduced the counts of yeast, mold and coliform bacteria ( P < 0.05) and, more importantly, alleviated mycotoxin contamination ( P < 0.05). These results indicated that LGG could be a novel inoculant to improve the quality and safety of WPCS and FTMR.
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Foods
·
2019
·Vol. 8
·期 8
·308
·
IF 3.011
Huali Chen,Gengsheng Xiao,Yujuan Xu,Yuanshan Yu,Jijun Wu,Bo Zou
The aim of this study was to evaluate the effect of high hydrostatic pressure (HHP) and co-fermentation byLactobacillus rhamnosusandGluconacetobacter xylinuson the quality of yacon-litchi-longan (YLL) juice. The carbohydrates, organic acids, free amino acids (FAAs), and volatile compounds in YLL juice were analyzed. Thermal processing (TP) increased the content of total carbohydrates, organic acids and FAAs, and destroyed the aroma components, whereas HHP treatment had a negligible effect. Carbohydrate content was lower, and content of lactic acid, acetic acid, and exopolysaccharide (EPS) were higher in co-fermented juice than in unfermented juice. Furthermore, the content of bitter FAAs in fermented TP and HHP-treated YLL juices decreased by 88.7% and 86.9%, respectively. Co-fermentation also increased ketones and the sum of individual volatile constituents, and improved the overall flavor of juice. Taken together, HHP treatment prior to co-fermentation can be used to improve the quality of YLL juice, especially the flavor thereof.Keywords:high hydrostatic pressure;Lactobacillus rhamnosus;Gluconacetobacter xylinus;free amino acids;volatile flavor compounds
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yan Hu,Shengpeng Zhu,Xuexin Ye,Zhijie Wen,Hudie Fu,Jiasi Zhao,Mohan Zhao,Xinxi Li,Yuqing Wang,Xiaojun Li,Li Kang,Ainiwaer Aikemu,Xinzhou Yang
Probiotics regulate intestinal flora balance and enhance the intestinal barrier, which is useful in preventing and treating colitis. However, they have strict storage requirements. In addition, they degrade in a strongly acidic environment, resulting in a significant decrease in their activity when used as microbial agents. Lactobacillus rhamnosus GG (LGG) was loaded into acid-resistant and colon-targeting double-layer microgels. The inner layer consists of guar gum (GG) and low methoxyl pectin (LMP), which can achieve retention and degradation in the colon. To achieve colon localization, the outer layer was composed of chitosan (CS) and sodium alginate (SA). The formulation demonstrated favorable bio-responses across various pH conditions in vitro and sustained release of LGG in the colon lesions. Bare LGG survival decreased by 52.2 % in simulated gastric juice (pH 1.2) for 2 h, whereas that of encapsulated LGG decreased by 18.5 %. In the DSS-induced inflammatory model, LGG-loaded microgel significantly alleviated UC symptoms in mice and reduced inflammatory factor levels in the colon. Encapsulation of LGG improved its stability in acidic conditions, thus increasing its content at the colon lesions and reducing pathogenic bacteria. These findings provide an experimental basis and a technical reference for developing and applying probiotic microgel preparations.
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Nanomaterials
·
2021
·Vol. 11
·期 3
·803
·
IF 5.076
Penghui Nie,Mengqi Wang,Yu Zhao,Shanji Liu,Ling Chen,Hengyi Xu
The potential toxicity of titanium dioxide nanoparticles (TiO2NPs) to mammals has become a widespread concern. Young individuals exposed to TiO2NPs have a higher risk than adults. In this study, the protective effects ofLactobacillus rhamnosusGG (LGG) on liver toxicity in young rats induced by TiO2NPs were explored. Results show that the four-week-old rats that underwent LGG after the oral intake of TiO2NPs could prevent weight loss, reduce hematological indicators (WBC and NEUT) and serum biochemical indicators (AST, ALT, AST/ALT, and ALP). Moreover, it alleviated the pathological damage of the liver (as indicated by the disordered hepatocytes, more eosinophilic, ballooning degeneration, and accompany with blood cells), but it did not reduce the Ti contents in the liver. In addition, RT-qPCR results indicated that LGG restored the expression of anti-oxidative stress-related genes, such asSOD1,SOD2,CAT,HO-1,GSH,GCLC, andGCLMin the liver. In summary, the hepatotoxicity of TiO2NPs in young rats is closely related to oxidative stress, and the antioxidant effect of LGG might protect the harmful effects caused by TiO2NPs.Keywords:titanium dioxide nanoparticles;Lactobacillus rhamnosusGG;liver;toxicity;oxidative stress
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JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2016
·
IF 1.964
Zijian Zhao,Xiaona Xie,Zhi Wang,Yanchun Tao,Xuedun Niu,Xuri Huang,Li Liu,Zhengqiang Li
Lactic acid bacteria immobilization methods have been widely used for lactic acid production. Until now, the most common immobilization matrix used is calcium alginate . However, Ca-alginate gel disintegrated during lactic acid fermentation . To overcome this deficiency, we developed an immobilization method in which Lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by using elemental analysis. We also optimized the cultivation conditions of the immobilized L. rhamnosus and obtained a high glucose conversion yield of 92.4%. Furthermore, L. rhamnosus encapsulated in mesoporous silica-based material exhibited operational stability during repeated fermentation processes and no decrease in lactic acid production up to 8 repeated batches.
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危险品化学品经营许可证（带存储）