MACROMOLECULAR BIOSCIENCE
·
2021
·Vol. 21
·期 7
·2100078
·
IF 4.979
Chengde Liu,Liang Pan,Cheng Liu,Wentao Liu,Yizheng Li,Xitong Cheng,Xigao Jian
Tissue adhesion to bone implant and osteoblastic differentiation are the key factors to achieve poly(aryl ether ketone) (PAEK) implant osseointegration. However, physical interaction of implant with tissue and hydroxyapatite coating suffers from slow implant tissue integration and lack of long-term stability. In this study, a novel poly(phthalazinone ether sulfone ketone) containing allyl groups (APPBAESK) is coated onto PPBESK sheet for reacting with the allyl groups of the hydrogel coating to enhance its stability. N-Succinimidyl (NHS)-ester activated group and nano-hydroxyapatite (nano-HA) are introduced into the hydrogel synthesized from gelatin methacrylate (GelMA) and acrylic acid to construct a nanocomposite hydrogel coating on PPBESK which is a promising PAEK implant material. The hydrophilicity of the PPBESK sheet is improved by the hydrogel coating. The chemical components of the nanocomposite hydrogel coating are confirmed by X-ray photoelectron spectroscope, Attenuated total reflection infrared, and X-ray powder diffraction. The tissue shear adhesion strength of the hydrogel coating toward pig skin is enhanced due to the synergism of NHS-ester activated group and nano-HA. The osteogenic differentiation of MC3T3-E1 preosteoblasts is promoted by nano-HA in nanocomposite hydrogel coating. Therefore, the bifunctional nanocomposite hydrogel coating provides a great application prospect in the surface modification of PAEK implants in bone tissue engineering.
研究领域:
生物材料
·
组织工程
·
骨科
·
材料科学
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DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Wenqiang Li,Changpeng Xu,Lin Huang,Zhiguan Huang,Bin Zhou,Yuhe Li,Jianghui Dong,Liping Wang
Precise detection of Fe 3+ ions in vitro is important for diagnosis of disease and bone health. Covalent organic polymers (COPs) are chosen as the precision probes, due to their light-weight, stable-bonds, and fast-response. In this work, a new fluorescent COP, pyrene-2,7-diboronic-COP(PD-COP), was designed and its feasibility in detection of iron concentration in cells was tested. PD-COP showed good performance in selective detection of Fe 3+ , among other metal ions. Its high anti-interference property and recyclability provide the theoretical basis for practical applications. PD-COP could selectively detect Fe 3+ ions with low limit of detection (LOD) at a concentration of about 0.68 µM. The quenching mechanism could be due to the transfer of electron. The in vitro experiments using cells also showed that the PD-COP had good biocompatibility, and the ability to detect Fe 3+ ions in cells. In conclusion, we successfully synthesized a novel COP, with high sensitivity that could selectively detect Fe 3+ ions. The COP had the ability to enter the cell membrane and combine with iron ions, which provided a new method of monitoring bone health.
研究领域:
材料科学
·
分析化学
·
生物材料
·
细胞生物学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
Bioengineered
·
2021
·
IF 3.269
Xiaochen Wang,Yaochuan Mi,Wei He,Xiaona Hu,Shuo Yang,Lu Zhao,Yanyang Zhang,Binhong Wen
Diabetic osteoporosis (DOP) is a chronic complication of diabetes in the skeletal system. High level of miR-340-5p may be harmful to the bone formation. In this study, the DOP model of rats was successfully established via streptozotocin (STZ) and ovariectomy (OVX) treatment. It was manifested by reduced body weight, insulin level, alkaline phosphatase (ALP) activity, and osteocalcin (OCN) and collagen-I expressions, as well as increased concentration of fasting blood glucose. Moreover, we found that miR-340-5p expression was increased while runt-related transcription factor-2 (RUNX2) was decreased in femurs. Furthermore, the effects of miR-340-5p on osteogenic differentiation (OD) in high glucose (HG)-treated MC3T3-E1 cells were explored. Exposure to OD and HG contributed to elevated miR-340-5p level. Inhibition of miR-340-5p enhanced ALP level, calcium deposition, and OCN, collagen-I and RUNX2 levels. On the contrary, miR-340-5p overexpression reversed these promotional effects. Luciferase assay indicated that RUNX2 may be a target gene of miR-340-5p. Moreover, RUNX2 deficiency decreased miR-340-5p inhibition-induced ALP activity, calcium accumulation and OCN, collagen-I, RUNX2 levels. In short, the above findings revealed that inhibition of miR-340-5p facilitated osteogenic differentiation through regulating RUNX2 in MC3TC-E1 cells, which provided targeted therapeutic strategies for the treatment of DOP.
研究领域:
分子生物学
·
内分泌学
·
骨生物学
·
糖尿病研究
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产品关联
DOI
PubMed
高影响因子
BMC Complementary Medicine and Therapies
·
2024
·Vol. 24
·期 1
·1-15
·
IF 3.3
He Yu,Liu Fei,He Mingjuan,Long Fayu,Hu Ding,Chen Jingwen,Fang Miao,Wang Zhenlong
The purpose of this study was to investigate the mechanism by which resveratrol (Res) inhibits apoptosis and promotes proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells, laying the groundwork for the treatment of osteoporosis (OP). The TCMSP database was used to find the gene targets for Res. The GeneCards database acquire the gene targets for OP. After discovering the potential target genes, GO, KEGG, and Reactome enrichment analysis were conducted. Verifying the major proteins involved in apoptosis can bind to Res using molecular docking. CCK8 measured the proliferative activity of mouse pre-osteoblasts in every group following Res intervention. Alkaline phosphatase staining (ALP) and alizarin red staining to measure the ability of osteogenic differentiation. RT-qPCR to determine the expression levels of Runx2 and OPG genes for osteogenic differentiation ability of cells. Western blot to measure the degree of apoptosis-related protein activity in each group following Res intervention. The biological processes investigated for GO of Res therapeutic OP involved in cytokine-mediated signaling pathway, negative regulation of apoptotic process, Aging, extrinsic apoptotic signaling pathway in absence of ligand, according to potential therapeutic target enrichment study. Apoptosis, FoxO signaling pathway, and TNF signaling pathway are the primary KEGG signaling pathways. Recactome pathways are primarily engaged in Programmed Cell Death, Apoptosis, Intrinsic Apoptotic Pathway, and Caspase activation via extrinsic apoptotic signaling pathways. This research established a new approach for Res treatment of OP by demonstrating how Res controls the apoptosis-related proteins TNF, IL6, and CASP3 to suppress osteoblast death and increase osteoclastogenesis.
研究领域:
药理学
·
细胞生物学
·
分子生物学
·
生物信息学
·
骨生物学
·
网络医学
✓ 已验证
DOI
高影响因子
Biomedical Reports
·
2024
·Vol. 21
·期 2
·1-8
·
IF 2.3
Naoki Nanashima,Kayo Horie,Indrawati Oey
Osteoporosis risk increases in menopausal individuals owing to the decrease in estrogen secretion. Blackcurrant extract (BCE) ameliorates osteoporosis; however, the underlying mechanisms are unclear. Furthermore, although BCE has phytoestrogenic activity, its effects on osteoblasts are unknown. In the present study, we investigated BCE‑mediated attenuation of osteoporosis using mouse MC3T3‑E1 pre‑osteoblasts, with a focus on osteogenesis. After treating MC3T3‑E1 cells with BCE for 48 h, cell proliferation was assessed using Cell Counting Kit‑8. Levels of osteoblast differentiation markers, namely alkaline phosphatase activity and total collagen content in the cells, were evaluated after 3 and 14 days of BCE treatment, respectively. The expression of genes encoding osteoblast differentiation markers, including collagen type I (Col‑I), alkaline phosphatase (Alp), bone γ‑carboxyglutamate protein (Bglap), and runt‑related transcription factor 2 (Runx2), was evaluated using reverse transcription‑quantitative polymerase chain reaction. Mineralization of the cells was evaluated using Alizarin Red staining. Femoral tissues of ovariectomized (OVX) rats with or without 3% BCE were stained using ALP to evaluate osteogenic differentiation in femoral tissue. After treating MC3T3‑E1 cells with BCE, cell proliferation had increased. BCE treatment increased Alp activity and total collagen content. Moreover, the expression of Col‑I, Alp, Bglap, and Runx2 increased in BCE‑treated cells. Furthermore, when MC3T3‑E1 cells were treated with BCE for 21 days, the levels of calcified nodules increased. Alp staining intensity was stronger in the epiphyses on femoral tissue of OVX rats treated with 3% BCE than in those of untreated OVX rats. The results suggest that BCE may promote osteogenesis by inducing osteoblast differentiation.
✓ 已验证
DOI
PubMed
高影响因子
IET Nanobiotechnology
·
2021
·Vol. 15
·期 9
·687-697
·
IF 1.847
Mingfu Ye,Linhu Wang,Zhang Wu,Wenjun Liu
The authors' previous study showed that zirconium oxide nanoparticles (ZrO 2 NPs) induce toxic effects in MC3T3-E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time-of-flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3-E1 cells in response to ZrO 2 NPs. The results demonstrated that MC3T3-E1 cells treated with ZrO 2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO 2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO 2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO 2 NPs in MC3T3-E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO 2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO 2 NP dental materials.
研究领域:
纳米技术
·
毒理学
·
细胞生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 8
·3732-3745
·
IF 6.1
Xinyan Liu,Ru Song,Rongbian Wei,Baoding Zhao,Yurou Chu
Vitamin D (VD) plays an important role in preventing osteoporosis. However, knowledge of the osteogenic effect of VD3 from shrimp processing by-products is limited. In this study, a VD3-rich extract from Penaeus sinensis processing by-products was prepared by saponification and liquid–liquid extraction combined with solid phase extraction for purification. The activity of purified VD3-rich extract (PPs-VD3) on MC3T3-E1, a preosteoblastic cell line, was determined. Furthermore, the improvement effect of PPs-VD3 on bone health of VD-deficient mice was investigated. PPs-VD3 stimulated the proliferation and differentiation of MC3T3-E1 cells. Compared to the same concentration of the VD3 standard, mineralization of MC3T3-E1 cells increased after 14 d or 21 d of PPs-VD3 treatment. Western blotting showed that PPs-VD3 significantly upregulated the protein levels of bone morphogenetic protein 2 and runt-related transcription factor 2 compared to the VD3 standard. Furthermore, PPs-VD3 treatment activated the receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) signaling pathway in MC3T3-E1 cells, especially increased OPG expression was detected at day 3 to day 14 compared to the VD3 standard treatment. More than ten medium and long chain fatty acids were detected in PPs-VD3, of which n-3 polyunsaturated fatty acids (PUFA) constituted 38.83 ± 8.61%, and the n-3/n-6 PUFA ratio in PPs-VD3 was 2.84 ± 0.23. The femoral trabeculae number and thickness of VD-deficient mice increased after 3 weeks of PPs-VD3 treatment. The changes of parameters associated with bone resorption including parathyroid hormone, bone mineral density and tartrate resistant acid phosphatase revealed the contribution of PPs-VD3 treatment in improving bone remodeling in VD-deficient mice. Our results suggest that PPs-VD3 could have potential prospects in alleviating osteoporosis or promotion of bone health.
研究领域:
营养学
·
药理学
·
细胞生物学
·
动物科学
✓ 已验证
DOI
PubMed
高影响因子
Experimental and Therapeutic Medicine
·
2018
·Vol. 16
·期 2
·643-648
·
IF 1.41
Sibo Li,Haitao Jiang,Xiaohua Gu
Glucocorticoids (GCs) are widely used to treat chronic diseases. Prolonged and/or overdose administration of GCs has many side‑effects to human health including GC‑induced osteoporosis (GIOP). In this investigation, the objective was to assess the influence that echinacoside (ECH) exerts upon dexamethasone‑treated murine osteoblastic MC3T3‑E1 cells. We found that ECH (5, 10, 20 and 40 mg/l) inhibited dexamethasone (1,000 nM)‑suppressed cell viability as demonstrated by Cell Counting Kit‑8 (CCK‑8) assay. The dose of 10 mg/l was selected for the following experiments because this dose had a better effect than the dose of 5 mg/l, and the doses >10 mg/l had a similar effect as this dose. ECH (10 mg/l) or pifithrin‑α (PFT‑α) (a p53 inhibitor, 20 µM) suppressed dexamethasone‑induced MC3T3‑E1 apoptosis as illustrated by Annexin V/propidium iodide (PI) double‑labeling flow cytometry analysis. ECH or PFT‑α treatment also alleviated dexamethasone's action of inhibiting Bcl‑2 expression as well as dexamethasone's action of stimulating on the expression of p53 and Bax. Moreover, lentivirus mediated‑p53 overexpression reversed the effects of ECH in dexamethasone‑treated MC3T3‑E1 cells, suggesting that ECH induced anti‑apoptotic effects in dexamethasone‑treated osteoblasts via p53‑dependent pathway. In summary, ECH has a protective effect against osteoblastic cell apoptosis induced by dexamethasone, suggesting that ECH may have potentials for clinical application in the treatment of GIOP.
研究领域:
药理学
·
细胞生物学
·
骨骼健康
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2017
·Vol. 7
·期 2
·162-169
·
IF 1.383
Guo Zhenzhao,Ma Min,Huang Xun,Li Hong,Zhou Changren
In the design of proper scaffolds for tissue engineering, the bulk material properties and scaffold architecture are fundamental because the scaffolds have been shown to have an essential role in the induction of cell adhesion and mediation of cellular behavior. It is popularly accepted
that the surface topography (shape, size, and surface texture) is one of the most vital cues that influences cellular reactions in vivo and cell-material interactions in vitro. The objective of this study was to better understand the impact of topography on MC3T3-E1 pre-osteoblasts'
response to the co-culture of electrospun fibrous scaffolds. Three kinds of biomimetic scaffolds with typical average fiber diameters of 0.347 μm, 0.947 μm, and 6.48 μ were fabricated by adjusting proper electrospinning conditions. Cell morphology, proliferation,
ALP activity, and gene expression of MC3T3-E1 cells cultured on the scaffolds were studied. Cells cultured on 0.35 m scaffolds showed a higher projected area and upregulation of osteogenic phenotype gene, such as Runx2, Col I, ALP, and OCN. For cells cultured on 6.5 μm scaffolds,
a higher proliferation rate and aspect ratio were noted. Our results showed that topographical cues designed into scaffolds can regulate proliferation and differentiation of osteoblastic cells.
研究领域:
组织工程
·
生物材料科学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2019
·Vol. 15
·期 12
·2351-2362
·
IF 0
Xu Yingjie,Wu Xin,Wang Shuyi,Yang Changzhou,Li Ying,Cao Yi
Hydrogels have been widely used to mimic the biochemical and mechanical environments of native extracellular matrices for cell culture and tissue engineering. Among them, self-assembling peptide hydrogels are of special interest thanks to their great biocompatibility, designability
and convenient preparation procedures. In pioneering studies, self-assembling peptide hydrogels have been used for the culture of bone marrow cells. However, the low mechanical stability of peptide hydrogels seems to be a drawback for these applications, as bone marrow cells prefer hard substrates
for osteogenic differentiation. In this work, we explored the use of hydroxyapatite (HAP)-peptide hybrid hydrogels for three-dimensional (3D) culture and differentiation of osteogenic MC3T3-E1 cells. We used HAP nanoparticles as crosslinkers to increase the mechanical stability of peptide
hydrogels. Meanwhile, HAP provided unique chemical cues to promote the differentiation of osteoblasts. A phosphate group was introduced to the self-assembling peptide so that the peptide fibers could bind to HAP nanoparticles specifically and strongly. Rheological characterization indicated
that the hybrid hydrogels were mechanically more stable than the hydrogels containing only peptides and can be used for long term cell culture. Moreover, the hydrogels were biocompatible and showed very low cytotoxicity. The favorable mechanical properties of the hybrid hydrogels and the chemical
properties of HAP synergistically supported the differentiation of MC3T3-E1 cells. Based on these characterizations, we believe that these hybrid hydrogels can potentially be used as scaffolds for cartilage and bone regeneration in the future.
研究领域:
生物材料
·
组织工程
·
细胞生物学
·
材料科学
✓ 已验证
DOI
PubMed
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Cuixia Yu,Junjun Zhuang,Lingqing Dong,Kui Cheng,Wenjian Weng
Hierarchical porous bioglass films on the tantalum were designed to enhance osteointegration of metallic implants. The films were prepared by a sol-gel method using P123 as the mesopore template and polystyrene microsphere as the nanopore template. The films with 5.4 nm mesopores and 100 nm nanopores (MBG-100) elicited an obviously elongated morphology of the cultured MC3T3-E1 cells, as a result, a higher alkaline phosphatase level was expressed. It is suggested that the nanopores play an important role in regulating cellular behavior by initial protein adsorption through nanopore curvatures. The mesopores were proven very effective for loading rhBMP-2, and the rhBMP-2 loaded on MBG-100 films showed a better function of enhancing osteogenic differentiation, which is attributed to that the nanopore structure could expedite rhBMP-2 release and provide a microenvironment for intensifying the interaction of rhBMP-2 with the cells. Hence, the cell osteogenic differentiation can be enhanced by hierarchical porous bioglass films through both the porous structure and rhBMP-2 induction.
研究领域:
生物材料
·
细胞生物学
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Kena Ma,Dan Huang,Jing Cai,Xinjie Cai,Lingling Gong,Pin Huang,Yining Wang,Tao Jiang
Metal orthopedic implants still face challenges in some compromised conditions, partly due to bio-inertness. The present study aimed to functionalize metallic implants with organic-inorganic nanocomposite (strontium-containing chitosan/gelatin) coatings through a simple single-step electrophoretic deposition under mild conditions. The surface characterization and in vitro cellular response were studied and compared with chitosan/gelatin (CS/G) coatings. SEM images suggested the inorganic nanoparticles may be encapsulated within or mixed with organic polymers. The XRD patterns showed that strontium carbonate was generated in the coatings. The TEM images revealed strontium-containing nanoparticles were released from the coatings in PBS. The continuous release after the initial burst release ensured the enduring effects of the functionalized surface. The tensile bond strength of the coatings to the substrates increased after the addition of strontium. In vitro cellular study confirmed that strontium-containing coatings supported the proliferation of MC3T3-E1 cells and exhibited excellent ability to enhance the differentiation of such pre-osteoblasts. Therefore, such organic-inorganic nanocomposite coatings are a promising candidate to functionalize orthopedic implant surfaces.
研究领域:
生物材料学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）