TALANTA
·
2020
·
IF 5.339
Xingxing Yang,Jiadi Sun,Fangchao Cui,Jian Ji,Liping Wang,Yinzhi Zhang,Xiulan Sun
N-acylated homoserine lactones (AHLs), a class of auto-inducers produced by Gram-negative bacteria, are typical signaling molecules in quorum sensing (QS) systems. Importantly, AHLs play a key role in determining the virulence of foodborne pathogens and reflect the activity of spoilage bacteria. In this study, an eco-friendly fluorescence-sensing platform for the rapid and sensitive detection of AHLs was developed and characterized. Molecularly imprinted polymers embedded with yellow-emitting carbon quantum dots (CQDs) were obtained via the sol-gel process using furanone as an alternative template molecule, and long-wave-emitting CQDs with excellent optical properties were used as signal conversion materials. After template elution, the blotting cavities on the surface of the CQD@MIPs (molecularly imprinted polymers) were able to selectively recognize AHLs, demonstrating a stronger fluorescence response compared with the corresponding CQD@NIPs (non-imprinted polymers). Under optimal test conditions, a good linear relationship between the concentration of analyte and the relative fluorescence intensity of the CQD@MIPs was observed. The linear detection range was 0–2.0 μM, and the limit of detection (LOD) was 0.067 μM. Importantly, the proposed sensing platform functioned as an optical detection strategy that responded quickly (2 min) to AHLs. Additionally, this sensing platform was applied to the analysis of AHLs in bacterial supernatant samples with satisfactory results. More interestingly, the 3D-printing CQD@MIPs were tentative explored in this work, which was personalized and portable, has an advantage of point of care testing (POCT) detection in the future. Based on these results, this detection strategy has demonstrated substantial potential for application in and the field of food safety.
研究领域:
食品科学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 6
·1051-1057
·
IF 1.248
Dong Yanying,Yu Ping,Sun Qilong,Lu Yang,Tan Zhenjiang,Yu Xiaopeng
Effective molecularly imprinted membranes(MIMs) were developed as an efficient adsorbent for the selective removal of p -hydroxybenzoic acid( p -HB) from acetylsalicylic acid(ASA, aspirin). The MIMs were grafted successfully from poly(vinylidene fluoride) microfiltration membranes via reversible addition-fragmentation chain transfer(RAFT) polymerization. The graft copolymerization of acrylic acid(AA) in the presence of template p -hydroxybenzoic acid led to molecularly imprinted polymer(MIP) film coated membranes. The obtained MIMs were characterized by scanning electron microscopy(SEM), Fourier transform infrared spectrophotometer(FTIR) and Raman spectra, and batch mode adsorption studies were carried out to investigate the specific adsorption equilibrium, kinetics and selective recognition properties of different MIMs. The kinetic properties of the MIMs could be well described by the pseudo-second-order rate equation. Selective permeation experiments were performed to evaluate the permeation selectivity of the p -HB imprinted membranes. The observed performances of the MIMs are applicable to the further purification of aspirin.
研究领域:
材料科学
·
化学工程
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Xianhui Cheng,Yujuan Miao,Juntao Zhou,Feng Lu,Jingji Jin,Lianghai Hu
Plasma membranes not only maintain the intracellular microenvironment through their phospholipid bilayer but also eliminate exogenous compounds outside the cell membranes. Most drugs especially with high polarity are prevented from entering into cells to exert their effects. Therefore, it is of great significance to design effective drug carriers with a penetrating ability toward plasma membranes. In this study, a dual-templated MIP (dt-MIPs) carrier with controllable microstructure and high drug loading capacity was prepared using highly expressed sphingomyelin on the plasma membrane and tenofovir (TFV), a first-line drug for HIV and chronic hepatitis B, as template molecules. The drug release experiments performed in vitro under simulated physiological conditions demonstrated that sustained and stable adsorption of TFV on dt-MIPs was more than 80% over 50 h. By a combination of flow cytometry and confocal microscopy, dt-MIPs were found to have efficient cell permeability. Furthermore, mass-spectrometry-based intracellular pharmacokinetic studies demonstrated that TFV was delivered completely into cells within 30 min with the delivery of dt-MIPs. The study presented above suggested that dt-MIPs are expected to be alternative nanoscale drug carriers for enhanced drug permeability and controlled release.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Xiaohan Wang,Jingru Wang,Wenshi Zhao,Rui Guo,Sicheng Cui,Jie Huang,Jiaxin Lu,He Liu,Yang Liu
Tetracycline (TC) is widely distributed in environment and a significant environmental pollutant. It has been challenging to rapidly identify and effectively remove TC contaminants. This work produced a multifunctional Fe 3 O 4 -Cu 2 O-Au-MIPs nanocomposite (FCAu-MIPs NCs) based on molecular imprinting technology for selective SERS detection and photocatalytic degradation of TC. We synthesized FCAu NCs with various Au contents and analyzed the relationship between Au content and SERS performance using finite-difference time-domain (FDTD) technique. Exceptional SERS performance could be attributed to the dense distribution of multiple hotspots on the SERS substrate. The photocatalytic mechanism of FCAu-MIPs NCs towards TC was also discussed. The combination of the narrow-bandgap semiconductor Cu 2 O and noble metal Au with localized surface plasmon resonance (LSPR) effect facilitated efficient light absorption and enhanced charge carrier transfer efficiency. Both SERS detection limits as low as 10 −9 M and selective photocatalytic degradation of TC in soil were achieved. Furthermore, FCAu-MIPs NCs were successfully recovered and reused with assistance of external magnetic field. After six cycles, outstanding SERS and photocatalytic capacities from FCAu-MIPs NCs could still be maintained. The FCAu-MIPs NCs with selective recognition ability to TC should thus hold tremendous applicative possibilities for highly sensitive detection and efficient degradation of TC.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2026
·
IF 6.1
Zhi Sun,Donghui Wang,Nan Wang,Juan Jin,Yiming Zhang
The development of new technologies for at-home self-testing holds promising potential for chronic disease management and monitoring. Recently, microfluidic paper-based analytical devices (μPADs) have demonstrated versatile capabilities for point-of-care testing (POCT). Sialic acid (SA), closely linked to the modification of glycoproteins in vivo, serves as an important biomarker for home-based self-testing. In this study, a novel paper-based microfluidic biomimetic chip (FP@Zr-BA@MIP) was successfully developed using boronate affinity surface imprinting technology. This chip exhibited a high adsorption capacity (Q e = 352.83 mg g −1 ), good selectivity (imprinting factor, IF = 3.67), rapid detection efficiency (within 30 min), and excellent anti-interference capability against sialic acid. Additionally, a visual sensing system was integrated into the platform, enabling rapid, visual, and quantitative analysis of SA. A linear range of 0.1–25 mg L −1 was achieved, with the limit of detection (LOD) and limit of quantitation (LOQ) as low as 0.066 mg L −1 and 0.21 mg L −1 , respectively. The synergistic combination of the simple operational features inherent to paper-based microfluidics and the intuitive readout advantage of visual sensing significantly enhanced the simplicity and overall efficiency of the detection process. This design offers a novel approach for realizing a POCT platform for non-invasive analysis of SA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2013
·Vol. 5
·期 17
·4494-4500
·
IF 1.855
Yang Wang,Jinhua Wu,Cheng Xue,Ruoyu Wang,Tingting Wen,Junli Hong,Qin Hu,Fei Li,Xuemin Zhou
A novel dendrimer-based molecularly imprinted polymers (MIPs) coated dumbbell-shaped stir bar (DSB) was developed to enrich and analyze two kinds of phosphodiesterase type 5 (PDE-5) inhibitors in health foods (Hfs). The MIPs, with vardenafil as the template molecule and methacrylic acid (MAA) as the functional monomer, were synthesized on the surface of the dendritic-grafting DSB which was filled with magnetic core as substrate. The MIPs-DSB possessed mechanical stability, high adsorption capacity and good selectivity for vardenafil and its analogue sildenafil. A method for the determination of vardenafil and sildenafil in Hfs was established by stir bar sorptive extraction (SBSE) coupled with HPLC-UV. The contents of vardenafil and sildenafil were found to be 3.65 and 2.66 μg g−1, respectively, and the average recoveries in spiked Hfs were 86.5–91.8% with a relative standard deviation (RSD) below 5%. All results revealed that the MIPs-DSB coupled with HPLC-UV could be applied to the highly selective extraction and sensitive determination of trace analytes in a complex matrix.
研究领域:
药物分析
·
食品安全
·
分子印迹技术
·
分离科学
·
药物代谢
·
分析化学
·
功能材料
✓ 已验证
DOI
高影响因子
Small
·
2018
·Vol. 15
·期 1
·1803913
·
IF 9.598
Jixiang Wang,Jiangdong Dai,Yeqing Xu,Xiaohui Dai,Yunlei Zhang,Weidong Shi,Börje Sellergren,Guoqing Pan
Paper-based assays for detection of physiologically important species are needed in medical theranostics owning to their superiorities in point of care testing, daily monitoring, and even visual readout by using chromogenic materials. In this work, a facile test strip is developed for visual detection of a neurotransmitter dopamine (DA) based on dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs). The DE-MIPs, featured with tailor-made DA affinity and good anti-interference, exhibit DA concentration-dependent fluorescent colors, due to the variable ratios of dual-emission fluorescence caused by DA binding and quenching. By facile coating DE-MIPs on a filter paper, the DA test strips are obtained. The resultant test strip, like the simplicity of a pH test paper, shows the potential for directly visual detection of DA levels just by dripping a tiny amount of biofluid sample on it. The test result of real serum samples demonstrates that the DA strip enables to visually and semiquantitatively detect DA within 3 min by using only 10 µL of serum samples and with a low detection limit ((100–150) × 10 −9 m ) by naked eye. This work thus offers a facile and efficient strategy for rapid, visual, and on-site detection of biofluids in clinic.
研究领域:
医学诊断
·
分析化学
·
生物传感器
·
治疗诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiang-Yun Lin,Dong-Yang Tian,Zeng-Qiang Wu,Ning Bao
Electrochemical sensors based on molecularly imprinted polymer (MIP) provide an exceptional platform for sensitive and selective analysis of analytes in complex matrix through incubation while in vivo analysis is critical for achieving quantitative information for the biological study in real time. However, the deployment of MIP electrochemical sensors for in vivo analysis highly depends on their forms and fabrication. Here blade-style MIP electrochemical sensors were designed and fabricated in batches based on the technique of lamination, focusing on their applications for in vivo analysis of trace indole-3-acetic acid (IAA) in plants. The blade-style electrochemical sensors were fabricated using stainless steel sheets as supporting and conductive substrates for the working electrodes. The MIP sensors were prepared in batches using cyclic voltammetry (CV) based electropolymerization of pyrrole (the functional monomer) and IAA (the template) followed by elution under a constant potential at a multichannel electrochemical station with improved throughput. After optimization, the sensors could stably and reproducibly quantify IAA with the detection limit of 0.1 μmol·L −1 . The blade-style MIP sensors could be either plugged in tomato fruits for in vivo analysis of IAA or coupled with a piece of hydrogel for non-invasive analysis of IAA in tomato leaves. The blade-style MIP electrochemical sensors may provide a platform for in vivo analysis by not only obviating the time-consuming sample treatment but also simplifying the analytical procedure. This study may pave the way for extensive applications of MIP electrochemical sensors for real-time, spatially resolved analysis of endogenous biomolecules in living organisms in the near future.
研究领域:
分析化学
·
植物生理学
·
电化学
·
生物传感器
·
分子印迹技术
·
化学生物学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Tao Wen,Weitao He,Qiyi Nie,Zhongliang Gong,Dapeng Li,Liang Wen,Hailong Peng
The characteristic substances in citrus volatile organic compounds (VOCs) are associated with the infestation of the Bactrocera dorsalis (Hendel), which provides a noninvasive evaluation method to discriminate infested citrus. This paper developed an olfactory detection system based on a quartz crystal microbalance (QCM) sensor array and classification algorithms to identify the citrus infested with B. dorsalis . Six characteristic substances, including d -limonene, myrcene, α-pinene, decanal , linalool , and β-ocimene, which vary significantly after B. dorsalis infestation were selected as template molecules to prepare molecularly imprinted polymers (MIPs) and modify QCM respectively. The experimental results show that the prepared MIPs-QCM sensors had sensitivity in the range of 0.043–0.070 Hz/(mg/m 3 ), and their stability and reproducibility were above 93.1%. Four sensors that contributed to the classification were screened by a stepwise discriminant analysis. Afterward, Bayesian optimization was employed to optimize the hyperparameters. The accuracy of the optimized support vector machine (SVM) reached 94.17%. The olfactory detection system developed in this study enables the discrimination of citrus infested with B. dorsalis , which may have potential applications in the field of post-harvest treatment of citrus.
研究领域:
植物保护
·
食品科学与技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Mengmeng Zhang,Wei Zhou,Qingqing Wang,Ning Wang,Xin Wang,Youping Liu,Xin Di
In this work, a novel water compatible core–shell magnetic molecularly imprinted nanocomposite (Fe3O4–COOH@MIPs) was synthesized in an acetonitrile–water reaction system by adopting carboxyl group-functionalized Fe3O4 nanoparticles as the magnetic core, olaquindox as the template molecule and acrylamide as the functional monomer. The resulting Fe3O4–COOH@MIPs was characterized and applied as an adsorbent for magnetic solid-phase extraction (MSPE) of three quinoline-1,4-dioxides (QdNOs). The Fe3O4–COOH@MIPs possessed strong magnetic responsiveness, fast adsorption equilibrium rate (equilibrium within 5 min) and excellent selectivity. By coupling Fe3O4–COOH@MIPs based MSPE with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), good linearity (R2 ≥ 0.9985) and low limits of detection (0.83–5.0 ng L−1) for QdNOs were obtained. Finally, the proposed method was applied to determine trace QdNOs in environmental water samples, and the recoveries were in the range of 68.08–99.88% with RSD less than 12.5%.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Kwabena Agyarko Sarpong,Kun Zhang,Yu Luan,Yunfei Cao,Wanzhen Xu
Tetrabromobisphenol-S (TBBPS) is a Brominated Flame Retardants (BFRs). TBBPS in trace quantities is lethal and do not only alter the normal performance of the endocrine system but can directly or indirectly provoke hepatoxicity and carcinogenic diseases. Herein, we fabricate a facile electrochemical sensor and investigate its application towards the determination of TBBPS through imprinting technique. TBBPS imprinting was carried out in the presence of methacrylic acid (MAA) and 4-aminothiophenol (4-ATP) functional monomers (DiM) over gold nanoparticles (AuNPs) modified carbon paste electrode (CPE). Transmission electron microscopy (TEM) was used to characterize the imprinted sensor. Performance was thoroughly investigated using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The proposed sensor showed detection behavior towards TBBPS in standard solutions with different concentrations range of 0.1 nM-10 nM and with a detection limit of 0.029 nM. The developed MIP(DiM)@AuNPs/CPE sensor had enhanced recognition capacity compared to MIP electrochemical sensors developed with a mono functional monomer (MIP(MoM)@AuNPs/CPE) and non-imprinted sensors. In addition, the low cost; easier preparation; excellent stability; and good selectivity of MIP(DiM)@AuNPs/CPE electrochemical sensor makes it an attractive alternative to high instrumentation detection techniques.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Wenjuan Wang,Yeqing Xu,Xiqing Liu,Long Peng,Ting Huang,Yongsheng Yan,Chunxiang Li
Ratiometric fluorescence imprinting sensors (GQDs/CdTe@MIPs) were successfully constructed by precipitation polymerization method for the recognition and detection of oxytetracycline (OTC) in milk samples. GQDs/CdTe@MIPs were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), fourier transform infrared (FT-IR), thermal gravity analysis (TGA), UV–visible spectroscopy (UV–vis) and X-ray photoelectron spectroscopy (XPS) techniques. A satisfying linear relationship under 0–80 μM OTC concentration (R 2 = 0.99699) was exhibited. High sensitivity was showed with the limit of detection of 3.5 nM and high recovery ranged from 99.93% to 100.20%. In addition, the selectivity of GQDs/CdTe@MIPs to OTC was well comparing with other antibiotics. With the injection of different concentrations of OTC, the fluorescent color change of GQDs/CdTe@MIPs under the 365 nm UV lamp further verified that GQDs/CdTe@MIPs were used successfully for the visual detection to OTC in milk samples.
研究领域:
分析化学
·
材料科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）