SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Guotai Yang,Xiangyu Meng,Yutong Wang,Mingyao Yan,Zoraida P. Aguilar,Hengyi Xu
A novel 2-step lectin-magnetic separation (LMS) method combined with gold nanoparticle (AuNPs) -based colorimetric system for specifically and quantitatively detecting S. aureus in blood samples was established. In this study, wheat germ agglutinin lectin from Triticum vulgaris (wheat) (WGA) was applied for the first time as a recognition agent in a 2-step magnetic separation method using magnetic nanoparticles (MNPs) for effective and low-cost bacteria enrichment. A quantitative detection strategy based on the long dsDNA amplicons stabilized with AuNPs against salt-induced aggregation was investigated. The 2-step LMS method resulted in a high enrichment efficiency even at low concentrations (10 0 –10 2 CFU/mL) of S. aureus in pure culture and blood samples. The AuNPs-based colorimetric system was applied for quantitative detection of S. aureus with a limit of detection (LOD) at 3.5 × 10 0 CFU/mL. The complete process starting with the blood samples treatment, 2-step LMS, and AuNPs-based colorimetric detection took only 7 h. The low cost and sensitive 2-step LMS combined with AuNPs-based colorimetric detection holds considerable potential for early sepsis diagnosis.
研究领域:
生物技术
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纳米技术
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微生物学
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临床诊断
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产品关联
DOI
高影响因子
Food Science and Human Wellness
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2023
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IF 7
Chen Chen,Yingfang Zhang,Ximo Wang,Xuguang Qiao,Geoffrey I.N. Waterhouse,Zhixiang Xu
Herein, a novel interference-free surface-enhanced Raman spectroscopy (SERS) strategy based on magnetic nanoparticles (MNPs) and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine. A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe 3 O 4 @Au MNPs (as the recognize probe for histamine) and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile (4-MBN) (Ag@4-MBN@Ag-c-DNA) as the SERS signal probe for the indirect detection of histamine. Under an applied magnetic field in the absence of histamine, the assembly gave an intense Raman signal at “Raman biological-silent” region due to 4-MBN. In the presence of histamine, the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe 3 O 4 @Au MNPs, thus decreasing the SERS signal. Under optimal conditions, an ultra-low limit of detection of 0.65 × 10 -3 ng/mL and a linear range 10 -2 –10 5 ng/mL on the SERS aptasensor were obtained. The histamine content in four food samples were analyzed using the SERS aptasensor, with the results consistent with those determined by high performance liquid chromatography. The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.
研究领域:
分析化学
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纳米技术
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食品科学
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生物传感器
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2021
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IF 9.229
Hengyan Ma,Mengya Li,Ting Yu,Hui Zhang,Minghua Xiong,Feng Li
In the present study, a magnetic mimic multi-enzyme system was developed by encapsulating the aryloxyphenoxypropionate (AOPP) herbicide hydrolase QpeH and alcohol oxidase (AOx) in zeolitic imidazolate framework (ZIF-8) nanocrystals with magnetic Fe3O4 nanoparticles (MNPs) to detect AOPP herbicides. The structural, protein loading capacity and loading ratio, porosity, and magnetic properties of QpeH/AOx@mZIF-8 were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen sorption, and vibrating sample magnetometry. An AOPP herbicide colorimetric biosensor made with QpeH/AOx@mZIF-8 had the highest sensitivity toward quizalofop-P-ethyl (QpE) with a limit of detection of 8.2 μM. This system was suitable to detect two other AOPP herbicides, including fenoxaprop-P-ethyl (FpE) and haloxyfop-P-methyl (HpE). The practical application of the biosensor was verified through quantitative analysis of QpE residues in industrial wastewater and field soils. Furthermore, QpeH/AOx@mZIF-8 exhibited excellent long-term storage stability (at least 50 days), easy separation by magnet, and reusability (at least 10 cycles), supporting its promising role in simple and low-cost detection of AOPP herbicides in real environmental samples.
研究领域:
分析化学
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环境科学
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生物传感器
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
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IF 3.131
Bowen Zhang,Yufei Wang,Jiao Zhang,Sanyuan Qiao,Zhenzhong Fan,Jiaqi Wan,Kezheng Chen
The application of magnetite (Fe 3 O 4 ) nanoparticles (MNPs) as adsorbent materials in solving wastewater treatment problems has attracted increasing attention. In this work, well-defined 3-Aminopropyltriethoxysilane (APTES) functionalized MNPs (APTES-MNPs) were synthesized by two-step method, in which a simple and scalable polyol method was used to initially synthesize MNPs with relatively large sizes and provide a defect site enriched surface to enable the APTES to coat on Fe 3 O 4 uniformly without using SiO 2 coating. The structure, morphology and properties of the APTES-MNPs were studied by TEM, XRD, FTIR, XPS and MPMS. The APTES-MNPs showed enhanced adsorption capacity towards HPAM due to the presence of high density of amino groups on the surface. Kinetics study revealed that the adsorption process followed the pseudo-second-order model, whereas the equilibrium isotherm data matched well with the Langmuir model. The APTES-MNPs could be rapidly separated from aqueous solution under magnetic field and be repeatedly used up to three cycles without significant loss of adsorption capacity. This new type of APTES-MNPs is expected to be a promising candidate for HPAM removal from aqueous solution.
研究领域:
材料科学
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环境科学
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纳米技术
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化学工程
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水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）