ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Xueting Wang,Guangchen Huang,Qirui Chen,Ruirui Pang,Zhibang Han,Jundong Zhu,Bing Xie,Yinglong Su,Shuai Zhou
Sludge is one of the primary reservoirs of microplastics (MPs), and the effects of MPs on subsequent sludge treatment raised attention. Given the entry pathways, MPs would exhibit different properties, but the entry pathway-dependent effect of MPs on sludge treatment performance and the fates of antibiotic resistance genes (ARGs), another high-risk emerging contaminant , were seldom documented. Herein, MPs with two predominant entry pathways, including wastewater-derived (WW-derived) and anaerobic digestion-introduced (AD-introduced), were used to investigate the effects on AD performance and ARGs abundances. The results indicated that WW-derived MPs, namely the MPs accumulated in sludge during the wastewater treatment process, exhibited significant inhibition on methane production by 22.8%–71.6%, while the AD-introduced MPs, being introduced in the sludge AD process, slightly increased the methane yield by 4.7%–17.1%. Meanwhile, MPs were responsible for promoting transmission of target ARGs, and polyethylene terephthalate MPs (PET-MPs) showed a greater promotion effect (0.0154–0.0936) than polyamide MPs (PA-MPs) (0.0013–0.0724). Compared to size, entry pathways and types played more vital roles on MPs influences. Investigation on mechanisms based on microbial community structure revealed characteristics (aging degree and types) of MPs determined the differences of AD performance and ARGs fates. WW-derived MPs with longer aging period and higher aging degree would release toxics and decrease the activities of microorganisms , resulting in the negative impact on AD performance. However, AD-introduced MPs with short aging period exhibited marginal impacts on AD performance. Furthermore, the co-occurrent network analysis suggested that the variations of potential host bacteria induced by MPs with different types and aging degree attributed to the dissemination of ARGs. Distinctively from most previous studies, the MPs with different sizes did not show remarkable effects on AD performance and ARGs fates. Our findings benefited the understanding of realistic environmental behavior and effect of MPs with different sources.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qin Xiang,Lijuan Yao,Jiao Zhou,Songling Li,Wei Zeng,Ping Liu
The mechanism underlying effective binding between the aroma compounds in Zanthoxylum bungeanum oil and pork, especially during heating, remains unclear, restricting accurate regulation of numbing flavor in meat dishes. Therefore, this study investigated the effects of heating on the binding between pork myofibrillar proteins (MPs) and four key aroma-active compounds in Zanthoxylum bungeanum oil and the related mechanism. Results showed that higher temperatures within 70–90 °C induced larger particle sizes and zeta potential values of MPs while reducing endogenous fluorescence. The surface hydrophobicity and secondary structure increased when heating to 80 °C and subsequently declined. Besides aroma compounds, these structural changes of MPs also affected their affinity for aromatic compounds. Higher binding percentages were observed in 2,3-butanediol (36 %–90 %) and linalyl acetate (12 %–55 %), while heating at 80 °C increased their binding amounts. Further correlation analysis and molecular docking revealed that these aroma compounds primarily bound with MPs via hydrogen bonding and various hydrophobic interactions.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Meixuan Wu,Renshu Wang,Lin Miao,Pengfei Sun,Baocheng Zhou,Yubing Xiong,Xiaoping Dong
Pollution of microplastics (MPs) has been drastically threating human health, however, whose elimination from the environment by current approaches is inefficient due to their high molecular weight, strong hydrophobicity and stable covalent bonds. Herein, we report a novel and highly-efficient route to degrade MPs contaminants through synergistically piezocatalytic and Fenton-like activation of H 2 O 2 by a ferroelectric Bi 12 (Bi 0.5 Fe 0.5 )O 19.5 catalyst under ultrasound treatment. For 10 g/L polyethylene terephthalate microplastics (PET-MPs), the synergistic strategy reached a 28.9 % removal rate in 72 h, which is greatly enhanced in comparison to the individual piezocatalysis and Fenton (Fenton-like) activation. By optimizing the types of oxidants (H 2 O 2 , peroxymonosulfate and peroxydisulfate) and bismuth ferrite catalysts (non-piezoelectric Bi 2 Fe 4 O 9 and piezoelectric BiFeO 3 /Bi 12 (Bi 0.5 Fe 0.5 )O 19.5 ), it was revealed that H 2 O 2 is the best oxidant, and the piezoelectric Bi 12 (Bi 0.5 Fe 0.5 )O 19.5 with a high aspect-ratio morphology showed higher activity than the Bi 2 Fe 4 O 9 and BiFeO 3 . The catalyst dosage and H 2 O 2 concentration were further optimized, and the good durability of the catalyst was also demonstrated through multiple uses. Different characterization technologies demonstrated the occurrence of PET-MPs oxidation and fragmentation during the treatment process. The plausible mechanism of synergistically piezocatalytic and Fenton-like H 2 O 2 activation was proposed based on measurements of band structure, piezoelectric property and reactive oxygen species generation. Finally, we detected the intermediates and determined a possible degradation route of PET-MPs. The toxicity assessment indicated that the produced intermediates have low toxicity and potential risks to the environment.
研究领域:
环境科学
·
催化化学
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2021
·
IF 5.275
Lian Xu,Li-Chao Wang,Bing-Mei Su,Xin-Qi Xu,Juan Lin
(2 S , 3 R )-4-methylsulfonylphenylserine [(2 S , 3 R )-MPS], a key chiral precursor for antibiotics florfenicol and thiamphenicol, could be asymmetrically synthesized by l -threonine transaldolase (LTTA) coupled with an acetaldehyde elimination system. The low efficiency of acetaldehyde elimination system blocked further accumulation of (2 S , 3 R )-MPS. To address this issue, strengthening acetaldehyde elimination system and enzyme self-assembly strategy were combined to accelerate biosynthesis of (2 S , 3 R )-MPS. The new multi-enzyme cascade with intensified acetaldehyde elimination system BL21 (PsLTTAD2/ScADH/BtGDH) could produce (2 S , 3 R )-MPS with a titer of 157.6 mM, 1.7-folds than that produced by the original system BL21 (PsLTTAD2/ApADH/CbFDH). Moreover, self-assembly of PsLTTAD2 and ScADH by respective fusion of SpyTag and SpyCatcher were carried out to develop a self-assembled multi-enzyme cascade BL21 (ST-PsLTTAD2/SC-ScADH/BtGDH). As a result, the yield of (2 S , 3 R )-MPS was up to 248.1 mM with 95% de . As far as we knew, that represented the highest yield of (2 S , 3 R )-MPS by enzymatic synthesis, and therefore was a promising and green route for industrial production of this valuable compound.
研究领域:
生物催化
·
酶工程
·
生物技术
·
化学生物学
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DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
·
2021
·
IF 2.604
Chang Liu,Mengdi Sheng,Tingting Wei,Jihong Sun,Shiyang Bai,Xia Wu
The fluorescent temperature-sensitive mesoporous materials with core-shell structured feature were synthesized through self-assembly method, in which, bimodal mesoporous SiO2 materials (BMMs) were used as a core and poly(N-isopropyl acryl-acrylamide)-co-poly(acrylic acid) as a shell. Especially, the 2-[3-(triethoxysilyl) propyl-1H-Benz][de]isoquinoline-1,3(2H)-dione (PID) as fluorescent molecule was cooperated into the BMMs by one-pot (doped) or two-step (grafted) methods. Using ibuprofen as a model drug, and the PID-doped P@BMMs and PID-grafted P@BMMs as the carriers, the drug releasing performances show the strong fluorescent properties and temperature-responsive behaviors. These results suggest that the prepared fluorescent nanocomposites should be a promise drug carrier for a potential drug delivery.
研究领域:
材料科学
·
生物医药
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DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2026
·
IF 4.4
Yanan Zhang,Yumu Luo,Zhensi Meng,Jun Luo,Ke Li,Yuhang Lin,Minle Li,Rongren Bin,Mingming Fu,Ming Hung Wong
The widespread use of agricultural films has resulted in the pervasive accumulation of microplastics (MPs) and phthalates (PAEs) in soil, posing significant ecological toxicity risks to soil organisms. In this study, we investigated the toxic responses of Eisenia fetida following single and combined exposure to polyethylene microplastics (PE-MPs) and dibutyl phthalate (DiBP), covering survival, growth inhibition, oxidative stress, stress-related gene transcription, and histopathology. At the early exposure phase, co-stimulation by PE-MPs and DiBP markedly inhibited core antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT), while triggering sharp compensatory elevation of glutathione S-transferase (GST) activity. Such antioxidant imbalance triggered early lipid peroxidation and cell membrane injury, evidenced by elevated malondialdehyde (MDA) concentrations. Although partial recovery of antioxidant enzyme activity was observed at day 28, obvious oxidative DNA damage was detected. This DNA injury disturbed genomic homeostasis, as reflected by significantly upregulated transcription of calreticulin (CRT) and heat shock protein 70 (Hsp70), alongside suppressed expression of translationally controlled tumor protein (TCTP). Two-way ANOVA verified prominent interactive effects between PE-MPs and DiBP at the gene transcription level, with synergistic and antagonistic effects alternating under different concentration combinations. At the tissue level, the combined exposure eventually induced severe pathological lesions in the epidermis, muscle layers and intestinal wall, accompanied by obvious body weight loss in earthworms. Collectively, these results provide mechanistic insights into the interactive toxic effects of coexisting PE-MPs and DiBP, and offer fundamental data to support ecological risk evaluation of mixed microplastic–plasticizer pollution in farmland soils.
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 11
·7517-7527
·
IF 3.9
Yihong Yang,Genlan Ye,Xiaozhong Qiu
Intraoperative bleeding is a pivotal factor in the initiation of early recurrence and tumor metastasis following breast cancer excision. Distinct advantages are conferred upon postoperative breast cancer treatment through the utilization of locally administered implant therapies. This study devised a novel 3D sponge implant containing cisplatin-loaded chitosan–calcium alginate MPs capable of exerting combined chemotherapy and hemostasis effects. This innovative local drug-delivery implant absorbed blood and residual tumor cells post-tumor resection. Furthermore, the cisplatin-loaded chitosan–calcium alginate MPs sustainably targeted and eliminated cancer cells, thereby diminishing the risk of local recurrence and distant metastasis. This hydrogel material can also contribute to breast reconstruction, indicating the potential application of the 3D sponge in drug delivery for breast cancer treatment.
研究领域:
肿瘤学
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药学
·
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Longji Zhu,Ruilong Li,Kai Yang,Fei Xu,Chenshuo Lin,Qinglin Chen,Dong Zhu,Qian Sun,Yong-Guan Zhu,Li Cui
Microplastics (MPs) ubiquitous in environments promote the dissemination of antibiotic resistance genes (ARGs), threatening ecosystem safety and human health. However, quantitative assessments of the health risks of ARGs (HRA) in plastisphere and an in-depth exploration of their driving mechanisms are still lacking. Here, the microbiomes, ARGs, and community assembly processes of five types of MPs in an urbanizing watershed were systematically investigated. By fully considering the abundance, clinical availability, human pathogenicity, human accessibility, and mobility of 660 ARGs in plastisphere, the HRA on MPs were quantified and compared. Polyethylene had the highest HRA among the five MP types, and urbanization further increased its risk index. In addition to abiotic factors, more complex biotic factors have been shown to drive HRA in plastisphere. Specifically, dispersal limitation accounted for the increasing diversity and interaction of bacteria that determined HRA in plastisphere. Further analysis of metabolic functions indicated that a higher HRA was accompanied by decreased normal metabolic functions of plastisphere microbiota due to the higher fitness costs of ARGs. This study advances the quantitative surveillance of HRA in plastisphere and understanding of its driving mechanisms. This will be helpful for the management of both MPs and ARGs treatments for human health.
研究领域:
环境科学
·
微生物学
·
公共卫生
·
生态学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Aying Wen,Guoliang Li,Di Wu,Yanxin Yu,Yan Yang,Na Hu,Honglun Wang,Jian Chen,Yongning Wu
Most of the reported covalent organic frameworks (COFs) are hydrophobic, limiting their adsorption application in sample pretreatment field. In this work, sulphonate functionalized magnetic covalent organic frameworks (COFs) composites were first synthesized by loading gold nanoparticles on Fe 3 O 4 @COF(TpBD) surface and then functionalized by sodium 3-mercaptopropanesulphonate immobilization via Au-S bonding formation (denoted as Fe 3 O 4 @COF(TpBD)@Au-MPS nanocomposites), which were further utilized as adsorbents for magnetic solid-phase extraction (MSPE) of fluoroquinolones. Compared with Fe 3 O 4 @COF(TpBD), the composites exhibited higher affinity to fluoroquinolones. Under optimized conditions, the developed MSPE method coupled with HPLC–MS/MS showed good linearity (R 2 ≥0.9989) and yielded low limits of detection (0.1–1.0 μg kg− 1 ) for fluoroquinolones. Moreover, the proposed method was successfully applied to extract fluoroquinolones from spiked meats (pork, chicken and bovine). The satisfactory recoveries were in the range of 82–110.2% with the relative standard deviations (RSDs) lower than 7.7%. These results indicated that the Fe 3 O 4 @COF(TpBD)@Au-MPS is a promising magnetic adsorbent for trace fluoroquinolones determination in meat samples. This work not only provided a facile strategy for COF functionalization, but also developed an efficient method for detecting fluoroquinolones in foodstuffs.
研究领域:
分析化学
·
材料科学
·
食品安全
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 3.3
Haijun Chang,Yuanwei Shi,Jie Xiong,Zhaoying Bo,Yan Dai
Summary This study investigated the effects of the combination of Gaphalium affine extract (GAE) and sodium pyrophosphate (PP) on the structure and oxidative stability of porcine myofibrillar proteins (MPs) under oxidative stress. The results showed that GAE was rich in total flavonoids and total phenols (37.09% and 67.50%, respectively). GAE had strong free radical scavenging ability, especially for • OH, and the semi-inhibitory concentration (IC 50 ) of • OH reached as high as 3.59 μg/mL, which was slightly lower than that of the standard antioxidant vitamin C (V c ). The addition of GAE and PP significantly inhibited the formation of carbonyl groups compared to the control group ( P < 0.05). The presence of GAE further promoted the loss of total sulphydryl groups by 3.52% compared to the oxidative control group ( P < 0.05). However, PP and PP+G significantly inhibited the loss of total sulphydryl groups ( P < 0.05). The further reduction in the carbonyl content, increase in the hydrogen bond content, inhibition of the conversion of sulphydryl groups to disulphide bonds, and decrease in the cross-linking aggregation of corresponding proteins all indicate that GAE and PP could cooperatively regulate the oxidation of MPs. An appropriate amount of PP effectively modified the damage caused by GAE on both the secondary and tertiary structure of MPs to maintain their structural stability, however, a relatively higher concentration of PP led to a substantial degree of MP collapse and affected the quality of the proteins. These results suggest that the oxidative stability of MPs can be improved by the addition of an appropriate amount of GAE (0.2 mg/g protein) combined with PP (1 mM).
研究领域:
食品科学
·
生物化学
·
蛋白质化学
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xinle Han,Jing Xu,Yanfeng Shi,Lixingzi Wu,Ling Fu,Weina Xue,Yanhao Zhang,Xu Zhang,Xuli Jing,Zhibin Zhang
Microplastics and PFOA are emerging contaminants that are widely present in groundwater, posing threats to human health and ecosystems. This study aimed to investigate the remediation of polystyrene microplastics (PS-MPs) and perfluorooctanoic acid (PFOA) in groundwater using waste coconut shell biochar in permeable reactive barriers (PRBs). Fixed-bed column experiments were conducted with different bed depths, flow rates, initial pollutant concentrations, and co-contamination conditions. Results demonstrated that coconut shell biochar exhibited an effective adsorption capacity, with an initial concentration of 1 mg/L, flow rate of 0.5 mL/min, and bed depth of 2.5 mm, removal efficiencies of 75.00 % for PFOA and 36.24 % for PS-MPs were achieved within 10 days. Increasing the depth of biochar from 1.5 mm to 5.0 mm enhanced the removal efficiencies (PFOA:96.43 %, PS-MPs:61.72 %) and extended breakthrough times (480 min for PS-MPs, 2410 min for PFOA). Lower flow rates (0.2 mL/min) extended hydraulic retention time, improving adsorption (both PFOA and PS-MPs were > 75 %), while higher pollutant concentrations accelerate PRB depletion and reduce removal efficiency. Additionally, the presence of PS-MPs exhibited dual effects on PFOA remediation: promoting adsorption at low concentrations but causing competitive inhibition at high concentrations. Meanwhile, increasing PFOA concentrations intensified electrostatic repulsion, which reduced the removal efficiency of PS-MPs from 36.24 % to 10.43 % as the PFOA concentration increased from 0 to 10 mg/L. Furthermore, among the tested models (Thomas, Yoon-Nelson and BDST), the Thomas model best fitted the fixed-bed adsorption data for PS-MPs, PFOA, and composite contamination.
研究领域:
环境科学
·
水处理技术
·
吸附工程
·
新兴污染物修复
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liangting Shao,Jingwen Wang,Haijing Hu,Xinglian Xu,Huhu Wang
Microbial spoilage of meat products is a significant problem in the food industry. Aeromonas salmonicida is a significant microorganism responsible for spoilage in chilled meat. Its effector protein, hemagglutinin protease (Hap), has been identified as an effective substance for degrading meat proteins. The ability of Hap to hydrolyze myofibrillar proteins (MPs) in vitro demonstrated that Hap has obvious proteolytic activity, which could alter MPs’ tertiary structure, secondary structure, and sulfhydryl groups. Moreover, Hap could significantly degrade MPs, focusing primarily on myosin heavy chain (MHC) and actin. Active site analysis and molecular docking revealed that the active center of Hap was bound to MPs via hydrophobic interaction and hydrogen bonding. It may preferentially cleave peptide bonds between Gly44-Val45 in actin, and Ala825-Phe826 in MHC. These findings suggest that Hap may be involved in the spoilage mechanism of microorganisms and provide crucial insights into the mechanisms of meat spoilage induced by bacteria.
研究领域:
食品科学
·
微生物学
·
蛋白质化学
·
结构生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）