FOOD MICROBIOLOGY
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2026
·
IF 5.4
Qifeng Wu,Nana Zhang,Yichun Sun,Zhengfang Qi,Rongrong Zhang,Anyan Wen,Likang Qin,Haiying Zeng
Targeted enrichment of tocopherols in fungal fermentation systems remains a significant metabolic engineering challenge. This study presented a novel strategy employing homogentisic acid (HGA) modulation to efficiently enhance tocopherol production in the M. purpureus -fermented coix seed (MFCS) system. Three strategies of exogenous HGA supplementation (Exo-HGA), endogenous HGA accumulation (End-HGA), and the combination of exogenous HGA supplementation and endogenous HGA accumulation (Exo+End-HGA) were systematically investigated. While End-HGA optimally promoted lovastatin, pigment, and biomass, the Exo+End-HGA strategy (supplemented with 250 ug/mL Exo-HGA) achieved superior tocopherol enrichment. The contents of total, α-, and γ-tocopherol reached 225.407, 64.022, and 161.385 μg/mL, respectively, corresponding to a 1.86-fold increase in total tocopherol compared with the control group. A total of 2,425 differentially expressed metabolites were identified. The significant accumulation of HGA, concomitant increases in γ-tocotrienol and menatetrenone (VK2), along with a decline in ubiquinone precursors such as 4-hydroxybenzoic acid derivatives, suggested that HGA specifically activated the tocopherol biosynthesis pathway. KEGG pathway analysis further revealed that HGA upregulated the shikimate and methylerythritol phosphate/mevalonate (MEP/MVA) pathways, thereby coordinating the redistribution of carbon flux and repressing competing routes such as phenylpropanoid and alkaloid biosynthesis, ultimately facilitating tocopherol enrichment. This study elucidated the metabolic network underlying HGA-mediated tocopherol enrichment in MFCS, providing a the oretical foundation and potential targets for enhancing tocopherol production through microbial metabolic engineering strategies.
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PubMed
高影响因子
Current Research in Food Science
·
2022
·
IF 6.269
Liuming Xie,Gang Wang,Jianhua Xie,XianXiang Chen,Jiayan Xie,Xiaoyi Shi,Zhibing Huang
The exopolysaccharides (EPS) produced by the edible medicinal fungus Monascus purpureus (EMP) become the center of growing interest due to their techno-functional properties and their numerous applications in the food industries; however, the low EPS yields limit its application. In this study, the effect of genistein supplementation on the production, rheological and antioxidant properties of EPS by M . purpureus and its biosynthesis mechanism were explored. The results indicated that the addition of genistein (3 g/L) generated a 110% and 59% increase in the maximum mycelial biomass and EPS yield, respectively. The genistein supplementation group (G-EMP) had higher molar percentages of Xyl and Man, and significantly decreased molecule weight and particle size of EPS, which resulted in stronger antioxidant effect and cell growth promotion. Rheological analysis showed that both EMP and G-EMP demonstrated pseudoplastic fluid behavior and G-EMP exhibited strong gel-like elastic behavior (G' > G"). Furthermore, genistein not only facilitated the production of EPS by regulating cell membrane permeability, enhancing cellular respiratory metabolism and monosaccharide precursor synthesis pathways, and enhancing antioxidant enzyme activity to reduce oxidative stress damage, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways. These findings expand the application of M . purpureus resources and provide a paradigm for future study of the structural and functional characteristics of EPS.
研究领域:
微生物学
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食品科学
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生物技术
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PubMed
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2024
·
IF 3.9
Shuo Jiang,Siyuan Yuan,Youming Zuo,Tian Liu,Yufeng Zhou,Dianxing Wu,Qihe Chen,Xiaoli Shu
Rice high in resistant starch (RS) showed great benefits in preventing diabetes. Red mold rice (RMR) is a traditionally functional food in China, preparing RMR with high-RS rice might produce a novel RMR with better nutrients and promote the utilization of high-RS rice in the food industry. R4 with the highest RS content was the most prone to be fermented by Monascus . After fermentation, the total starch and protein contents were significantly decreased, while the RS, lipid, free glucan, total free amino acids (FAAs), and γ-aminobutyric acid (GABA) contents were remarkably increased. Concurrently, the phenolic content and antioxidation activity were enhanced greatly. The individual phenolic acids such as ferulic acid showed significantly positive correlations with the antioxidant activity. Besides the abundant lipids, FAAs, GABA, and strong antioxidant capacity, fermented R4 had the highest ferulic acid content. This study provided alternatives for producing novel RMR and widened applications of high-RS rice in innovative functional foods.
研究领域:
食品科学
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微生物学
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营养学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Changyan Dong,Yi Cheng,Meihui Zhang,Mianhua Chen,Zhongli Yan,Sumei Zhou,BenxuYang,Qingbin Guo,Changlu Wang,Shufen Wu
In this study, four Monascus pigments (ankaflavin, AK; monascin MS; rubropunctatin, O 1 ; monascorubrin, O 2 ) were proved to exhibit considerable anti-glycation properties in bovine serum albumin (BSA)-fructose model. AK (40.62 %) and MS (48.38 %) were found to exert lower inhibitory effects on the formation of fluorescent advanced glycation end products (AGEs) than aminoguanidine (59.4 %), while O 1 (90.64 %) and O 2 (93.82 %) displayed much stronger abilities. AK and MS could trap methylglyoxal (MGO) with maximum capture rates of 85.67 % and 84.90 %, respectively, and only mono-MGO adducts of them were detected. LC-Orbitrap MS/MS analysis revealed that four pigments significantly altered the type and reduced the number of the glycated sites and they all covalently bound to BSA, with O 1 and O 2 possessing high reactivity. Altogether, AK and MS suppressed fluorescent AGEs formation mainly via trapping MGO and covalently interacting with BSA, and blocking free amino groups was the dominant mechanism for O 1 and O 2 . These findings presented new insights into Monascus pigments as dietary supplement for inhibiting protein glycation.
研究领域:
生物化学
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食品科学
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药理学
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PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2026
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IF 4
Haoqun Liu,Yao Li,Wenjing Xu,Kan Shi,Da Song,Gong Chen
Phenolic content in oats was enhanced by enzyme hydrolysis coupled with Monascus fermentation (C-FEH). • 15 differential metabolites was identified enrich in L -phenylalanine/tyrosine pathways during C-FEH. • Possible biotransformation pathways of oat phenolics in C-FEH was revealed. Microbial fermentation and multi-enzyme systems can enhance the release and transformation of phenolic components in grains. This study constructed a system combining Monascus fermentation and multi-enzymatic hydrolysis to enrich phenolic content in oats. The mechanisms underlying phenolic release were explored using SEM, XRD, and FTIR analyses and non-targeted metabolomics was employed to investigate biotransformation pathways. Results demonstrated that the enzyme hydrolysis coupled with Monascus fermentation (C-FEH) system significantly promoted the release of oat phenolics, increasing their content from 1.05 mg GAE/g DW (unfermented) and 16.02 mg GAE/g DW ( Monascus fermentation alone) to 34.37 mg GAE/g DW. SEM and phase structure analyses revealed that C-FEH enhanced cellulose, hemicellulose, and lignin degradation, disrupting phenolic-cell wall interactions. Metabolomics identified 15 differential metabolites concentrated primarily in the L -phenylalanine and tyrosine pathways, promoting phenylpropanoid biotransformation. Phenolic bioactives such as vanillic acid, caffeic acid, p -coumaric acid, ferulic acid, naringenin, and genistein were significantly enriched. This study provides theoretical support for the functional food development of medicinal and edible grains.
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PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Xue Dong,Yongchao Cai,Hui Liao,Yao Wang,Zehua Chen,Yong Zhou,Jianrong Wu,Yi Luo,Fengyun Sheng,Zhemin Zhou,Xiaole Xia
Obesity and hyperlipidemia, chronic diseases resulting from excessive energy intake and abnormal lipid metabolism, respectively, are intricately associated with cardiovascular disease and diabetes and have become global health problems. Monascus ruber ( M. ruber ), a filamentous fungus with edible properties, produces bio-functional metabolites including a good deal of enzymes. In this study, the effect of the biological transformation of fermented food on obesity and hyperlipidemia, was systematically investigated using the mixture of medicine and food homology (MFH) hawthorn and millet as fermentation substrate. Results showed that fermentation promoted the release of bioactive compounds such as phenolic compounds. Pancreas lipase assay indicated that the inhibition rate and IC 50 of free phenolic compounds in fermented MFH food were 70.51% ± 2.59%, and 1.34 mg/mL, respectively. In vitro , the binding rates of free phenolic compounds in fermented MFH food to Sodium cholate (SC), sodium glycinate (SGC) and sodium taurocholate (STC) were 84.99% ± 6.59%, 80.29% ± 6.23%, and 79.68% ± 6.18%. Further, Monascus -fermented hawthorn and millet (MFHM) of fermented MFH food markedly suppressed lipid accumulation in oleic acid (OA)-induced HepG2, with cell triglycerides (TG) and total cholesterol (TC) clearance of 24.31% ± 1.81% and 73.88% ± 2.72%, respectively. These findings implied that the fermentation of a mixture of MFH millet and hawthorn by M. ruber holds promise for reducing obesity and lowering lipid levels, thereby serving as a valuable foundation and reference for future research on the development of multifunctional food products.
研究领域:
食品科学
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营养学
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药理学
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生物化学
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高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Chaoran Yang,Wei Yu,Boyan Gao,Yuge Niu,Liangli Yu
To improve the resistance of Monascus pigments (MPs) to adverse environmental factors, a nanoparticle carrier was constructed using ovalbumin, rutin and pectin modified by octenyl succinic anhydride. According to the determination of particle size, ζ-potential and microstructure, the composite nanoparticles exhibited the "core-shell" structure. Specifically, the ovalbumin core was encapsulated by the modified pectin adsorbed with rutin. Spectroscopic analysis demonstrated that electrostatic interactions, hydrogen bonding and hydrophobic interactions between wall materials and MPs were the main driving forces to form composite nanoparticles. The stabilities of light, thermal and storage of MPs were significantly enhanced after encapsulation due to the physical barrier effect of the wall materials against external disturbances and the multiple functions of rutin, including free radical scavenging, absorption of partial UV and scatter light, and possible co-pigmentation effects with MPs. Moreover, the composite nanoparticles exhibited higher inhibition activity against pancreatic lipase and antioxidant activity compared with those of free MPs. The results provided an ideal way to develop new MPs products, which had long-lasting and stable performance during food processing and storage.
研究领域:
食品科学
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纳米技术
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药学
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高影响因子
Food Bioscience
·
2025
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IF 5.9
Nana Zhang,Anyan Wen,Xin Yang,Xinxin Zhang,Likang Qin,Haiying Zeng
Homogentisic acid (HGA), a product of the L-tyrosine metabolic pathway, serves as a substrate for tocopherol synthesis. Previous studies revealed that the fermentation of coix seeds by M . purpureus could simultaneously upregulate tocopherol and HGA levels. Therefore, this study focused on HGA to investigate its specific effect on tocopherol production during the fermentation process. The results showed that 0.02% Exo-HGA significantly increased tocopherol yield by 53.11%, reaching 184.808 μg/g. Based on the seed culture medium, the optimal formulation of key factors for promoting End-HGA synthesis in M. purpureus was determined. It was obtained through single-factor experiments and uniform design: D-fructose (36.816 g/L), L-tyrosine (0.656%), yeast extract powder (7 g/L), ZnSO 4 (0.654 g/L). Under these conditions, M. purpureus exhibited good growth, with an End-HGA yield reaching 844.461 μg/mL. In addition, fermentation of coix seeds with End-HGA led to an increase in the total tocopherol yield by 57.36%, reaching 181.303 μg/g. This result was comparable to that achieved with Exo-HGA addition. Therefore, enhancing End-HGA synthesis is an effective strategy to achieve a high tocopherol yield through M. purpureus fermentation. In conclusion, this study demonstrated that the regulation of microorganisms can enable high-level synthesis of target products and improve the efficiency of deep conversion and utilization of plant substrates.
研究领域:
生物化学
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微生物学
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食品科学
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高影响因子
Foods
·
2025
·Vol. 14
·期 11
·1894
·
IF 5.1
Xiaomin Chen,Yao Hu,Zhen Zeng,Xu Zhang,Yahui Huang
As a potential raw material with a variety of bioactive compounds, Pu-erh raw tea can produce rich flavor and health benefits through natural fermentation or microbial fermentation in traditional processing. However, the traditional fermentation process has some problems such as a long fermentation period and unstable quality. Monascus, a kind of fungus used in both medicine and food, has been proved to have many beneficial effects such as lowering cholesterol and regulating blood lipids. Therefore, in this study we investigated whether the fermentation of mixed monascus could significantly improve the flavor quality and lipid-lowering activity of Pu-erh raw tea. We added four kinds of monascus to unfermented Pu-erh raw tea (UT) to obtain a fermented Pu-erh raw tea (FT). The quality of the two tea samples was determined and an in vitro lipid-lowering experiment was conducted. The results show that the contents of water extractives, flavone, trans-catechins (GCG and CG), theabrownins, and caffeine in FT are significantly higher than those in UT, increasing by 19.41%, 14.47%, 18.76%, 29.82%, and 10.67%, respectively. In terms of aroma, linalool was the characteristic compound of UT, presenting a floral note. D-Limonene was the key characteristic substance of FT, manifested as lemon, toast, and wood. In terms of taste, FT has a high content of bitter amino acids and caffeine, a low content of catechins, and is rich in carbohydrate substances, forming a characteristic mild and mellow slightly bitter taste with reduced astringency. In addition, the relative contents of active substances with lipid-lowering effects such as quercetin, quercitrin, ascorbic acid, and sorbitol in FT were higher than those in UT, increasing by 83.09%, 81.73%, 89.86%, and 92.76%, respectively. The effect of FT on regulating cellular lipid metabolism was superior to UT based on cell experiments. The research results provide a scientific basis for the deep processing and functional development of Pu-erh raw tea.
研究领域:
食品科学
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微生物学
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生物化学
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2026
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IF 13.8
Caoyu Guo,Chaoran Yang,Zimo Zhang,Boyan Gao,Yuge Niu,Yapeng Fang
As a natural pigment derived from fungi, Monascus pigments (MPs) was widely used in Asia. Its application is limited by poor color stability and distinct off-flavors. Here, a novel hydrogel named as HGP, composed by gelatin and pectin, was fabricated via dual cross-linking to encapsulate MPs to address these two shortcomings simultaneously. HGP showed the dense and interpenetrating microstructure confirmed by SEM, FT-IR, and TGA, with improved mechanical and hydration properties. Notably, the 8% HGP significantly improved color stability of MPs under thermal (100 °C, 7.5 h) and light (7000 lx, 6 days) stress. Volatile profiling by GC-TOF-MS identified 25 kinds of key odorous substances in MPs that were significantly reduced in 8-HGP-MPs. Sensory experiment demonstrated that 8-HGP significantly attenuated the characteristic fruity, fatty, musty, woody, alcoholic, and overall odor intensity of MPs. Correlation analysis further confirmed that the effective off-flavor masking of HGP-MPs was achieved by substantially lowering the concentrations of key volatiles of MPs. This work presents a food-grade dual-function carrier that overcomes the shortcomings of MPs, which expanded its use in food products.
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高影响因子
RSC Advances
·
2018
·Vol. 8
·期 31
·17389-17398
·
IF 2.936
Yunquan Zheng,Qisheng Pan,Liuda Mo,Wenyi Zhang,Yunjian Duan,Chengqun Chen,Haijun Chen,Yanghao Guo,Xianai Shi,Jianmin Yang
Alzheimer's disease (AD) is an extremely complex disease, characterized by several pathological features including oxidative stress and amyloid-β (Aβ) aggregation. Blockage of Aβ-induced injury has emerged as a potential therapeutic approach for AD. Our previous efforts resulted in the discovery of Monascus pigment rubropunctatin derivative FZU-H with potential neuroprotective effects. This novel lead compound significantly diminishes toxicity induced by Aβ(1-42) in Neuro-2A cells. Our further mechanism investigation revealed that FZU-H inhibited Aβ(1-42)-induced caspase-3 protein activation and the loss of mitochondrial membrane potential. In addition, treatment of FZU-H was proven to attenuate Aβ(1-42)-induced cell redox imbalance and Tau hyperphosphorylation which caused by okadaic acid in Neuro-2A cells. These results indicated that FZU-H shows promising neuroprotective effects for AD.
研究领域:
神经科学
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药理学
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分子生物学
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Zhenfeng Huang,Huixin Xie,Tingting Hu,Jiarui Zeng,Xiaofei Tian,Zhenqiang Wu
Natural Monascus yellow pigments (MYPs) have received considerable attention due to their great safety and biological activities, but their unsatisfactory color tonality and stability limit their applications. A novel encapsulate system for hydrophilic yellow pigments (HYPs) were investigated through stable ternary nanocomplexes using quercetin-Sn (II), bovine serum albumin (BSA), and chitosan (CS) in this work. Layer-by-layer assembly preparation of HYPs-loaded quercetin-Sn (II)-BSA-CS nanocomplexes were verified by fluorescence spectroscopy, fourier transform infrared spectroscopy , X-ray photoelectron spectra, and 1 H nuclear magnetic resonance. A hyperchromic effect on HYPs was obtained since their maximum ultraviolet absorption wavelength redshifted from 352 nm to 364 nm first due to the interaction with quercetin-Sn (II) and further redshifted to 408 nm due to ternary conjugation, greatly improved the color tonality and dye-uptake. The HYPs-loaded nanocomplexes showed irregular glomerate nanosphere with 252–382 nm of particle size. Their conjugation remitted HYPs degradation under various environmental stresses (thermal, light, and ascorbic acid treatment), and had a great color stability response to pH change. Higher lipid-lowering and antioxidant activities were detected in the nanocomplexes than free HYPs. This study provides a new way for fabricating novel MYPs with great physicochemical stability, bright color and hypolipidemic activities.
研究领域:
食品科学
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材料科学
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生物技术
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药学
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高影响因子
危险品化学品经营许可证（带存储）