PLoS One
·
2017
·Vol. 12
·期 1
·e0170013
·
IF 2.806
Sheng Liu,Wenjie Gao,Yao Wang,Zhenyu He,Xiaojun Feng,Bi-Feng Liu,Xin Liu
Monitoring glycosylation of the mAbs have been emphasized and routinely characterized in biopharmaceutical industries because the carbohydrate components are closely related to the safety, efficacy, and consistency of the antibodies. In this study, the comprehensive glycan profiling of a biosimilar candidate of cetuximab was successfully characterized using Normal phase high-performance liquid chromatography (NP-HPLC) in combination with Matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The presence of minor N-linked glycans containing sialic acid lactone residues (NeuAcLac) was observed in the biosimilar for the first time, which could influence the quantitative analysis of sialylated glycans and interfere with quantification of neutral glycans when it was analyzed by high performance liquid chromatography fluorescence (HPLC-FL). To overcome this issue, mild alkali treatment was used to hydrolyze lactone of the sialic acid to their neutral formation, which had no impact on the analysis of other glycans before and after the treatment. As a result, the mild alkali treatment might be helpful to obtain quantitative glycan profiling of the mAbs drugs with enhanced accuracy and robustness.
研究领域:
生物制药
·
蛋白质组学
·
药物分析
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 114
·113298-113306
·
IF 3.289
Yan Yuan,Bo Peng,Hang Chi,Cong Li,Ren Liu,Xiaoya Liu
We report the preparation of poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles (NPs) and graphene oxide (GO) ink-based layer-by-layer inkjet printing humidity sensors. Sulfonate polystyrene:PEDOT NPs (SPS:PEDOT NPs) were prepared with hard template SPS NPs and GO was synthesized through a modified Hummers' method. Next, the SPS:PEDOT NP/GO multilayer films were printed layer by layer, with one layer of GO followed by one layer of SPS:PEDOT NP ink on the flexible poly-ethylene terephthalate (PET) substrate by using a home inkjet printer. The morphology and structure of the SPS:PEDOT NPs and GO were characterized through TEM and Raman spectroscopy and those of the film were investigated through optical microscopy. SPS:PEDOT NP-reduced GO (SPS:PEDOT NP/RGO) composite sensors were obtained after thermal annealing. To assess their humidity sensing properties, the sensors were exposed to a wide range of relative humidities (RH 0–98%) at room temperature. The SPS:PEDOT NP/RGO PET-based sensor exhibited superior humidity sensing properties in a wide response range (0.13–68.46%), short response and recovery time (39 and 57 s, respectively), and excellent repeatability and flexibility. The resistance of sensors was almost invariable even after folding 200 times. Overall, the SPS:PEDOT NP/RGO PET-based sensors can be used as humidity sensors.
研究领域:
材料科学
·
传感器技术
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DOI
高影响因子
International Journal of Nanomedicine
·
2018
·
IF 4.37
Zhen Zhang,Hanqing Qian,Jie Huang,Huizi Sha,Hang Zhang,Lixia Yu,Baorui Liu,Dong Hua,Xiaoping Qian
Background Red blood cell membrane-coated nanoparticle (RBCm-NP) platform, which consist of natural RBCm and synthetic polymeric core, can extend circulation time in vivo with an improved biocompatibility and stability of this biomimetic nanocarrier. To achieve better bioavailability of antitumor drugs that were loaded in RBCm-NPs, the functionalization of coated RBCm with specific targeting ability is essential. Bispecific recombinant protein anti-EGFR-iRGD, containing both tumor penetrating peptide (internalizing RGD peptide) and EGFR single-domain antibody (sdAb), seems to be an optimal targeting ligand for RBCm-NPs in the treatment of multiple tumors, especially colorectal cancer with high EGFR expression. Materials and methods We modified the anti-EGFR-iRGD recombinant protein on the surface of RBCm-NPs by lipid insertion method to construct iE–RBCm–PLGA NPs and confirmed the presentation of active tumor-targeting ability in colorectal cancer models with high EGFR expression when compared with RBCm–PLGA NPs. In addition, potential anti-tumor drug gambogic acid (GA) was loaded into the NPs to endow the antitumor efficiency of iE–RBCm–GA/PLGA NPs. It was simultaneously evaluated whether GA can reach better biocompatibility benefiting from the improved antitumor efficiency of iE–RBCm–GA/PLGA NPs in colorectal cancer models. Results We successfully modified anti-EGFR-iRGD proteins on the surface of biomimetic NPs with integrated and stable “shell–core” structure. iE–RBCm–PLGA NPs showed its improved targeting ability in vitro (multicellular spheroids [MCS]) and in vivo (nude mice bearing tumors). Besides, no matter on short-term cell apoptosis at tumor site (terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling [TUNEL]) and long-term tumor inhibition, iE–RBCm–GA/PLGA NPs achieved better antitumor efficacy than free GA in spite of the similar effects of cytotoxicity and apoptosis to GA in vitro. Conclusion We expect that the bispecific biomimetic nanocarrier can extend the clinical application of many other potential antitumor drugs similar to GA and become a novel drug carrier in the colorectal cancer treatment.
研究领域:
纳米技术
·
药学
·
肿瘤学
·
生物医学工程
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Zhimin Li,Dongming He,Yimin Zhou,Zi-Yu Zhang,Zhongai Hu,Xiaoquan Lu
Nonylphenol (NP) is an important fine chemical raw material and intermediate that is widely utilized in industry and may be distributed in aquatic ecosystems. Following its entry into the food and water cycles, it can subsequently enter the human body and potentially harm the human reproductive system. For the purpose of monitoring NP in water, it is thus essential to build a straightforward, affordable, and robust electrochemical sensor. Based on a two-step chemical modification proceeding and an electrostatic self-assembly effect, a double-modified β-cyclodextrin functionalized multiwalled carbon nanotube sensor (HE-β-CD-CTAC/F-MWCNTs) has been successfully constructed. It incorporates the excellent host–guest interaction ability of β-cyclodextrin and the high chemical activity of cetyltrimethylammonium chloride (CTAC), and the carbon nanotubes have an enormous particular surface area and strong electrical conductivity. The electrochemical oxidation reaction of NP with the sensor is controlled by a surface adsorption process of equal numbers of protons and electrons. In accordance with the optimized experimental parameters, the limit of detection (LOD) for the sensor is 0.13 μM, and it responds linearly to NP in the concentration range of 1–200 μM. Meanwhile, the sensor has excellent repeatability, stability, and immunity to interference. For the detection of NP in real water samples, the sensor also showed an excellent recovery rate (92.8%–98.5%) and relative standard deviation (1.16%–3.26%).
研究领域:
环境科学
·
分析化学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 67
·62302-62309
·
IF 3.289
Ziyang Wu,Haibo Lin,Yunze Wang,Xuemin Yu,Jinglong Li,Zhu Xiong,Yi Wang,Youju Huang,Tao Chen,Fu Liu
Poly(vinylidene fluoride) (PVDF) membranes have been widely applied to treat wastewater, however, the removal of toxic aromatic phenolic compounds remains a technical challenge due to the serious adsorption fouling and difficult degradation. Herein, we aimed to design a superhydrophilic PVDF membrane decorated with Au nanoparticles, which enhanced the rapid degradation of p-nitrophenol (4-NP). The superhydrophilic PVDF membrane with a micro/nano structured surface was decorated with Au nanoparticles via poly(dopamine) (PDA) as a spacer. The influences of membrane affinity (e.g. Hydrophilic Membrane (HM), micro/nano structured superhydrophilic membrane (MSiM), and micro/nano structured superhydrophobic membrane (MSoM)) on PDA deposition and the subsequent Au decoration were comprehensively investigated. The synthesized Au nanoparticles were characterized using transmission electron microscopy (TEM) and UV-vis absorption spectra. The morphology and composition was evaluated using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Static catalytic experiments demonstrated that MSiM degraded over 90% of 4-NP in 5 minutes with a kinetic reaction rate constant of 47.84 × 10−2 min−1 and high stability over 6 cycles. A membrane catalytic reactor (MCR) was designed to realize the continuous catalytic degradation of 4-NP with a kinetic reaction rate constant of 7 × 10−2 min−1.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
Biomedical Reports
·
2025
·Vol. 23
·期 3
·1-10
·
IF 1.9
Yue Zhou,Yi-Ran Yao,Yan Xu,Xin Rong,Yue-Feng Qiu,Ting-Yue Qi
The present study developed a novel injectable, biocompatible, and thermosensitive hydrogel system for ultrasound‑triggered drug release, using a chitosan/sodium β‑glycerophosphate/titanium dioxide nanoparticle (CS/β‑GP/TiO2 NP) composite. The inclusion of TiO2 NPs aimed to enhance ultrasound sensitivity, enabling precise therapeutic release to targeted tissues. The hydrogels displayed excellent injectability (maximum injection force <6.5 N) and gelled effectively at body temperature. Biocompatibility was confirmed through Cell Counting Kit‑8, LIVE/DEAD, and western blot assays. The present study demonstrated that hydrogels with TiO2 NPs significantly increased the release rates of sodium fluorescein and bovine serum albumin, affirming the role of TiO2 NPs as a sensitizer. This establishes the CS/β‑GP/TiO2 NP hydrogel as a promising system for ultrasound‑responsive drug delivery. Potential applications include drug delivery, tissue engineering, and wound healing, with the system offering controlled and targeted therapy under ultrasound stimulation. Future research will optimize hydrogel properties, assess in vivo performance, and evaluate long‑term stability and effectiveness in clinical settings.
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高影响因子
Diversity-Basel
·
2022
·Vol. 14
·期 9
·756
·
IF 3.029
Xiaodong Shan,Feifei Chen,Huikang Lin,Hangjun Zhang,Yuchi Zhong,Zhiquan Liu,Yan Li
Zinc oxide nanoparticles (ZnO-NPs) are commonly used in various commercial applications, causing toxic effects on organisms and destroying biodiversity, but information about their protective approaches remains unknown. This study aims to evaluate the protective effects of theaflavins (TFs) and epigallocatechin gallate (EGCG) against ZnO-NP-induced cytotoxicity in rat tracheal epithelial (RTE) cells. Herein, RTE cells were exposed to 100 μg/L ZnO-NPs for 12 h, then treated with 0, 10, 100, and 1000 μg/L TFs or EGCG for another 12 h; subsequently, oxidative stress, inflammation, and apoptosis analyses were conducted. Relative to the control groups, TFs and EGCG treatment significantly inhibited the levels of reactive oxygen species and malondialdehyde content. Exposure to 1000 μg/L TFs or EGCG treatment downregulatedcytochrome Cgene expression levels by 59.10% and 77.27%;Caspase 3gene expression by 50.03% and 60.01%;Caspase 8gene expression by 45.11% and 55.57%; andCaspase 9gene expression by 51.33% and 66.67%, respectively. Meanwhile,interleukin 1βandinterleukin 6,tumor necrosis factor-α, and the other inflammatory chemokines such asC-C motif chemokine 2andC-X-C motif chemokine 8expression were all gradually rescued after the addition of TFs or EGCG. These results imply that TFs or EGCG possibly ameliorated ZnO-NPs-induced toxicity through antiapoptotic, antioxidant, and anti-inflammatory effects. This study provides novel approaches which mitigate the emerging nanoparticle pollutant toxicity in organisms, which may potentially slow down the destruction of biodiversity.Keywords:ZnO-NPs;apoptosis;theaflavins;epigallocatechin gallate;toxicology;antioxidants
研究领域:
毒理学
·
药理学
·
环境科学
·
细胞生物学
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DOI
高影响因子
Catalysts
·
2020
·Vol. 10
·期 5
·533
·
IF 3.52
Jinjun Wen,Chunlei Huang,Yuhai Sun,Long Liang,Yudong Zhang,Yujun Zhang,Mingli Fu,Junliang Wu,Limin Chen,Daiqi Ye
CO2hydrogenation to methanol is one of the main and valuable catalytic reactions applied on Cu/ZnO-based catalysts; the interface formed through Zn migration from ZnO support to the surface of Cu nanoparticle (ZnOx-Cu NP-ZnO) has been reported to account for methanol synthesis from CO2hydrogenation. However, the accompanied reverse water gas shift (RWGS) reaction significantly decreases methanol selectivity and deactivates catalysts soon. Inhibition of RWGS is thus of great importance to afford high yield of methanol. The clear understanding of the reactivity of RWGS reaction on both the direct contact Cu-ZnO interface and ZnOx-Cu NP-ZnO interface is essential to reveal the low methanol selectivity in CO2hydrogenation to methanol and look for efficient catalysts for RWGS reaction. Cu doped plate ZnO (ZnO:XCu) model catalysts were prepared through a hydrothermal method to simulate direct contact Cu-ZnO interface and plate ZnO supported 1 wt % Cu (1Cu/ZnO) catalyst was prepared by wet impregnation for comparison in RWGS reaction. Electron paramagnetic resonance (EPR), XRD, SEM, Raman, hydrogen temperature-programmed reduction (H2-TPR) and CO2temperature-programmed desorption (CO2-TPD) were employed to characterize these catalysts. The characterization results confirmed that Cu incorporated into ZnO lattice and finally formed direct contact Cu-ZnO interface after H2reduction. The catalytic performance revealed that direct contact Cu-ZnO interface displays inferior RWGS reaction reactivity at reaction temperature lower than 500 °C, compared with the ZnOx-Cu NP-ZnO interface; however, it is more stable at reaction temperature higher than 500 °C, enables ZnO:XCu model catalysts superior catalytic activity to that of 1Cu/ZnO. This finding will facilitate the designing of robust and efficient catalysts for both CO2hydrogenation to methanol and RWGS reactions.Keywords:reverse water–gas shift reaction;CO2hydrogenation to methanol;Cu doping;direct contact Cu-ZnO interface;ZnOx-Cu NP-ZnO interface
研究领域:
催化化学
·
材料科学
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DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Min Xu,Zhayun Gong,Yangyang Wu,Zhiyong Zhu,Kun Wang,Hefeng Xu,Cong Yu,Xiaodong Chu
4-Nitrophenol (4-NP) pollution in the chemical, dye, and other industries poses significant risks to humans and aquatic organisms. In this study, we prepared metal oxide-supported gold nanoparticles (AuNPs/Al2O3, AuNPs/TiO2, and AuNPs/ZnO) and applied them to the catalytic reduction of 4-NP. The Bader charge and differential charge, dos and d band centers, work function, and reaction free energy of AuNP composites were analyzed through DFT calculations. The influence of metal oxide carriers on the catalytic activity of AuNPs was explored, which decreased in the following order: AuNPs/Al2O3 > AuNPs/TiO2 > AuNPs/ZnO. In addition, the catalytic reduction performance of these AuNP composites for 4-NP in the presence of NaBH4 was investigated. The results showed that the AuNPs/Al2O3 catalyst exhibited the best performance (3.62 ± 0.17 min–1), consistent with the theoretical calculations. Meanwhile, the AuNPs/Al2O3 catalyst exhibited good reusability. More relevantly, the catalytic reduction mechanism of metal oxide-supported AuNPs for 4-NP was proposed from both experimental and theoretical calculations.
研究领域:
催化化学
·
环境科学
·
纳米技术
·
材料科学
·
理论化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 3
·1348
·
IF 4.9
Yansen Li,Jingyao Chen,Chaojie Yang,Wenhao Fan,Qirong Chen,Nan Yang,Pingye Deng,Wenlei Zhai,Zhiyong Yan,Feng Wang
A novel photoelectrochemical (PEC) biosensor was proposed by preparing Au NP/MXene–BiOCl Moiré superlattice nanosheets as the probes. Upon irradiation with visible light, the probe exhibited excellent electrical conductivity as well as high photoelectric conversion efficiency. Benefitting from the excellent PEC property of the hybrid probe, sensitive and accurate detection of protein kinase activity was demonstrated with a limit of detection of 0.0029 U mL−1. This study verifies the great PEC potential of MXene hybrid nanomaterials.
研究领域:
材料科学
·
生物传感
·
分析化学
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2026
·Vol. 65
·期 35
·20379-20388
·
IF 4.6
Guo Yuxiao,Zhao Zhijin,Li Bin,Zhang Lin,Wang Zhipeng,Xu Chao
Neptunium (Np), a key actinide element in the nuclear
fuel cycle,
presents critical scientific challenges regarding its environmental
migration behavior and efficient separation technologies, which are
essential for nuclear waste safe disposal and resource recovery. This
study systematically investigates the aqueous coordination chemistry
of Np(V) with three representative small-molecule ligands, benzoic
acid, phenol, and salicylic acid, that model the coordination environment
of natural organic matter. Utilizing variable-temperature spectrophotometric
titration coupled with density functional theory (DFT) calculations,
we elucidated the composition, stability constants, and thermodynamic
parameters of the formed complexes. The coordination processes were
found to be endothermic and entropy-driven, with ligand affinity following
the order: salicylic acid ≫ phenol > benzoic acid. Salicylic
acid exhibited exceptional entropy gain and coordination stability
due to the chelating effect of its ortho-carboxylate-phenolate groups.
This work provides molecular-level insights into the coordination
mechanisms between Np(V) and oxygen-containing functional groups.
The obtained quantitative thermodynamic and structural data establish
a molecular-level reference framework for understanding the intrinsic
coordination behavior of Np(V) with representative oxygen-donor functional
groups. These fundamental insights may inform future assessments of
Np speciation in complex environmental media and provide a thermodynamic
basis for the rational design of functional materials for Np separation.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Caiyan Wan,Shidong Liu,Yv Chen,Fengxiu Zhang
The N P flame retardant ammonium salt of hydroxyethyl hexahydrotristriazine˗triphosphoric acid (AHTTPA) was prepared by a one–pot synthesis method under formaldehyde-free and solvent-free conditions. The AHTTPA was finished on the biomolecule of cotton by using the dip-roll-bake method. Nuclear magnetic resonance (NMR 1 H, 13 C, and 31 P) demonstrated that AHTTPA was successfully synthesized. The flame retardancy of AHTTPA-treated cotton was studied by limiting oxygen index (LOI), vertical flaming test (VFT), scanning electron microscopy (SEM), and cone calorimetry (CC). The results from these tests indicate that AHTTPA-treated cotton exhibited favorable flame retardancy and durability (the LOI value of 40%-treated cotton after 50 laundering cycles (LCs) was 29.8%), the flame was immediately extinguished after removal from the treated cotton, no smoldering or continued burning, the burned part formed a complete carbon frame and generally maintained its original morphology, the peak heat release rate (PHRR) and total heat release (THR) of AHTTPA-treated cotton fabric were significantly lower than pure cotton. Thermogravimetric analysis ( TGA ) results showed that AHTTPA improved the thermal stability of cotton. The breaking strength and softness of AHTTPA-treated cotton was also retained.
研究领域:
材料科学
·
纺织工程
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DOI
PubMed
高影响因子
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