JOURNAL OF LUMINESCENCE
·
2015
·
IF 2.719
ShaoZhen Wang,Jianguo Zhang,Hongqi Chen,Lun Wang
A new serum ferritin (SF) biosensor based on fluorescence resonance energy transfer (FRET) from Mn 2+ -doped NaYF 4 :Yb,Tm up-converting rare-earth nanophosphors (UCNPs, donor) to gold nanorods (GNRs, acceptor) was developed in the near-infrared (NIR) region. The as-prepared Mn 2+ -doped NaYF 4 :Yb,Tm UCNPs exhibited a strong emission at 807 nm in the NIR region and were modified with poly-(acrylic acid) (PAA) before conjugating with anti-SF (Ab 1 ). The negatively charged donor (Ab 1 -UCNPs) and the positively charged acceptor (GNRs) were mixed in close proximity due to electrostatic interactions, which quenched the fluorescence emission of the Mn 2+ -doped NaYF 4 :Yb,Tm UCNPs. However, added SF combines with the anti-SF because the binding affinity between SF and Ab 1 is stronger than the electrostatic interactions. Thus, the acceptor separated from the donor, and the fluorescence was restored. Under optimal conditions, the restored fluorescence exhibited a linear relationship with SF concentrations ranging from 0.23 to 16.88 ng/mL and a limit of detection as low as 0.12 ng/mL. The proposed method was also used to monitor SF in human serum samples.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Ji-Wei Shen,Cheng-Xiong Yang,Lu-Xi Dong,Hao-Ran Sun,Kai Gao,Xiu-Ping Yan
Rational design and fabrication of multimodal imaging nanoprobes are of great significance for in vivo imaging. Here we report the fabrication of a multishell structured NaYF4:Yb/Tm@NaLuF4@NaYF4@NaGdF4 nanoprobe via a seed-mediated epitaxial growth strategy for upconversion luminescence (UCL), X-ray computed tomography (CT), and magnetic resonance (MR) trimodal imaging. Hexagonal phase NaYF4:Yb/Tm is used as the core to provide UCL, while the shell of NaLuF4 is epitaxially grown on the core not only to provide an optically inert layer for enhancing the UCL but also to serve as a contrast agent for CT. The outermost NaGdF4 shell is fabricated as a thin layer to give the high longitudinal relaxivity (r1) desired for MR imaging. The transition shell layer of NaYF4 not only provides an interface to facilitate the formation of NaGdF4 shell but also inhibits the energy transfer from inner upconversion activator to surface paramagnetic Gd3+ ions. The fabricated multishell structured nanoprobe shows intense near-infrared UCL, high r1 value of 3.76 mM–1 s–1, and in vitro CT contrast effect. The multishell structured nanoprobe offers great potential for in vivo UCL/CT/MR trimodal imaging. Further covalent bonding of folic acid makes the multishell structured nanoprobe promising for in vivo targeted UCL imaging of tumor-bearing mice.
研究领域:
生物医学工程
·
材料科学
·
影像医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hongqi Chen,Wei Tang,Yunchun Liu,Lun Wang
In this work, a quantitative image analysis method based on cyanine dye-upconversion nanoparticles composite luminescent nanoprobe for the detection of nitrite was developed. The nanoprobe was constructed by combining the NaYF 4 :Yb,Tm@NaYF 4 upconversion nanoparticles (UCNPs) and the new cyanine dye IR-790. The upconversion nanoparticles transferred energy to IR-790, resulting in the luminescence quenching, while the luminescence of UCNPs was recovered after adding NO 2 − . The increase in photons was related to the concentration of NO 2 − . Under the optimal experimental conditions, the detection range was 0.20–140 μM and the limit of detection was 0.030 μM. The measurement for NO 2 − can be completed in 29 min. The method has the characteristics of fast response (~0.1 s), low sample consumption (10 μL) and powerful data support (550 frame time series images). Furthermore, the quantitative image analysis method was successfully applied for the analysis of nitrite in environmental water and food samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Yuxian Su,Li Liu,Shipeng Wen
Semiconductor photocatalysis is a promising strategy for wastewater treatment. However, a limited photoresponse range, rapid recombination of photoinduced charge carriers, poor stability, and other problems have posed obstacles to practical applications. Herein, a facile strategy to fabricate a near-infrared-driven broadband photocatalyst was developed by anchoring the core–shell nanostructure of NaYF4:Yb,Tm@NaYF4:Yb,Nd@TiO2 (Tm@Nd@TiO2) onto the surface of porous silica/carbon (mSC) electrospun fibers. The Tm@Nd@TiO2 nanoparticles (NPs) exhibited strong near-infrared light absorption and energy transfer from the NaYF4:Yb,Tm@NaYF4:Yb,Nd core to the TiO2 shell, triggering the photocatalytic reaction. In addition, the interconnected mesoporous structure and carbon network of Tm@Nd@TiO2/mSC was found to be beneficial in improving the adsorption capacity toward organic pollutants, promoting the separation of photogenerated carriers, and further improving the overall photocatalytic performance. The photocatalytic activity of Tm@Nd@TiO2/mSC nanocomposite was evaluated by degrading methyl orange solution in the visible-near-infrared and simulated sunlight regions. The Tm@Nd@TiO2/mSC nanocomposite exhibited excellent adsorption performance. The photocatalytic activity of Tm@Nd@TiO2/mSC was found to be 3 times higher than that of bare Tm@Nd@TiO2 over 60 min.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 10
·2675-2683
·
IF 3.431
Gao Ni,Ling Bo,Gao Zhongling,Wang Lun,Chen Hongqi
An electrochemiluminescence resonance energy transfer (ECL-RET) system that detects prostate-specific antigen (PSA) was created. Near-infrared (NIR)-emitting NaYF 4 :Yb,Tm/Mn upconverting nanoparticles (UCNPs) are used as donors, and gold nanorods (GNRs) are used as acceptors. The ECL was enhanced nearly threefold by Mn 2+ doping, with an emission peak appearing at an NIR wavelength of 808 nm. Anti-PSA 1 (Ab 1 ) was bound to the surfaces of UCNPs after being modified with poly(acrylic acid) (PAA). As for acceptors, cetyltrimethylammonium bromide (CTAB)-capped GNRs were treated with 11-mercaptoundecanoic acid (MUDA) and then conjugated with Anti-PSA 2 (Ab 2 ). When PSA was added, donors and acceptors were brought in close proximity through specific interactions of antibodies and antigens, resulting in high quenching efficiency levels. Under optimal conditions, the linear range of detection was 3.75–938 pg/mL for PSA ( R = 0.999), with a detection limit as low as 3.16 pg/mL. This method can be applied to detect PSA in human serums with satisfactory results.
An electrochemiluminescence resonance energy transfer system was developed for determination of prostate-specific antigen using near-infrared-emitting NaYF 4 :Yb,Tm/Mn upconverting nanoparticles as donors and gold nanorods as acceptors.
研究领域:
分析化学
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纳米技术
·
生物传感器
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Keyi Cheng,Jianguo Zhang,Liping Zhang,Lun Wang,Hongqi Chen
A highly sensitive luminescent bioassay for the detection of Salmonella typhimurium was fabricated using Mn 2 + -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs) as the donor and gold nanorods (Au NRs) as the acceptor and utilizing an energy transfer (LET) system. Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs with a strong emission peak at 807 nm were obtained by changing the doped ion ratio. Carboxyl-terminated Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs were coupled with S. typhimurium aptamers, which were employed to capture and concentrate S. typhimurium . The electrostatic interactions shorten the distance between the negatively charged donor and the positively charged acceptor, which results in luminescence quenching . The added S. typhimurium leads to the restoration of luminescence due to the formation of UCNPs-aptamers- S. typhimurium , which repels the UCNPs-aptamers from the Au NRs. The LET system does not occur because of the nonexistence of the luminescence emission band of Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs, which had large spectral overlap with the absorption band of Au NRs. Under optimal conditions, the linear range of detecting S. typhimurium was 12 to 5 × 10 5 cfu/mL (R = 0.99). The limit of detection for S. typhimurium was as low as 11 cfu/mL in an aqueous buffer. The measurement of S. typhimurium in milk samples was satisfied in accordance with the plate-counting method, suggesting that the proposed method was of practical value in the application of food security.
研究领域:
生物传感器
·
微生物学
·
纳米技术
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Liping Zhang,Bo Ling,Lun Wang,Hongqi Chen
An upconversion luminescence method was developed for the determination of glutathione (GSH), L -cysteine (Cys) or L -ascorbic acid (AA) based on redox reaction . We synthesized poly(acrylic acid) (PAA)-modified Mn 2+ -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs), and the luminescence of these UCNPs was effectively quenched due to their carboxyl groups coordinating with Fe 3+ to form a UCNPs/Fe 3+ system. GSH, Cys or AA reduced Fe 3+ to Fe 2+ , which induced the luminescence recovery of the UCNPs. Under the optimized conditions, wide linear concentration ranges from 0.25-300 μM for GSH, 0.5–875 μM for Cys and 0.5–350 μM for AA were found, and the detection limits (3 S/K) were 0.2 μM, 0.5 μM and 0.2 μM, respectively. Thus, the UCNPs/Fe 3+ system was successfully applied for sensing GSH, Cys or AA.
研究领域:
纳米技术
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Zhenli Qiu,Jian Shu,Dianping Tang
Titanium dioxide (TiO2; as a potential photosensitizer) has good photocurrent performance and chemical stability but often exhibits low utilization efficiency under ultraviolet (UV) region excitation. Herein, we devised a near-infrared light-to-UV light-mediated photoelectrochemical (PEC) aptasensing platform for the sensitive detection of carcinoembryonic antigen (CEA) based on core–shell NaYF4:Yb,Tm@TiO2 upconversion microrods by coupling with target-triggered rolling circle amplification (RCA). The upconversion microrods synthesized through the hydrothermal reaction could act as a photosensing platform to convert the near-infrared (near-IR) excitation into UV emission for generation of photoinduced electrons. The target analyte was determined on a functional magnetic bead by using the corresponding aptamers with a sandwich-type assay format. Upon target CEA introduction, a complex was first formed between capture aptamer-1-conjugated magnetic bead (Apt1-MB) and aptamer-2-primer DNA (Apt2-pDNA). Thereafter, the carried primer DNA by the aptamer-2 paired with linear padlock DNA to trigger the RCA reaction. The guanine (G)-rich product by RCA reaction was cleaved by exonuclease I and exonuclease III (Exos I/III), thereby resulting in the formation of numerous individual guanine bases to enhance the photocurrent of core–shell NaYF4:Yb,Tm@TiO2 upconversion microrods under near-IR illumination (980 nm). Under optimal conditions, the near-IR light-mediated PEC aptasensing system could exhibit good photoelectrochemical response toward target CEA and allowed for the detection of target CEA as low as 3.6 pg mL–1. High reproducibility and good accuracy were achieved for analysis of human serum specimens. Importantly, the near-IR-activated PEC aptasensing scheme provides a promising platform for ultrasensitive detection of other biomolecules.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2023
·
IF 3.1
Aihua Zhou,Jiaxin Yang,Yan Li,Chengguo Ming,Yuanxue Cai,Yumiao Pei
Luminescence thermometry has recently been gaining popularity in science and technology fields. However, most reported works have used the fluorescence intensity ratio (FIR) to obtain the temperature based on thermally coupled energy levels (TCLs), which have hindered the sensitivity of the technique and limited its applications. Here, NaYF4:Yb/Tm@NaYF4:Yb/Ho microcrystals were prepared using a hydrothermal method. Based on the non-thermally coupled energy levels (NTCLs) of the double luminescent ionic centers Tm3+ and Ho3+, the materials exhibited temperature-dependent luminescence in the range of 304 K to 574 K. The maximum sensitivities were found to be Sa = 0.135 K−1 at 574 K and Sr = 1.101% K−1 at 484 K based on red (Tm3+) and green (Ho3+) emission bands. This study has provided a promising new strategy to break the TCL restriction and has shown the material to have potential applications in luminescence thermometry.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2014
·
IF 4.384
Hongqi Chen,Yingying Guan,Shaozhen Wang,Yuan Ji,Mengqi Gong,Lun Wang
A homogeneous immunoassay for the sensitive and selective determination of trace amounts of α-fetoprotein (AFP, a cancer marker) by detection in the near-infrared (NIR) region based on luminescence energy transfer (LET) from NaYF4:Yb,Tm/NaGdF4 core–shell upconverting nanoparticles to gold nanorods (GNRs) is presented. The carboxyl-functionalized NaYF4:Yb,Tm/NaGdF4 core–shell upconverting nanoparticles (UCNPs) were excited by a 980 nm continuous wavelength laser, and its emission peak appeared at a near-infrared wavelength (∼804 nm). The carboxyl-functionalized upconverting nanoparticles were conjugated with the anti-AFP (Ab1) and acted as donor. GNRs with a high absorption band around 790 nm, which was overlapped the UCNPs emission, were synthesized and acted as the acceptor. The donor (negatively charged) interacted with the acceptor (positively charged) via electrostatic interactions to bring them into close proximity. LET could occur, producing a quenching phenomenon. When the AFP antigens were added into the system, the binding affinity between AFP and Ab1 was stronger than the electrostatic interactions, which released the energy acceptors from the energy donors, interrupting luminescence energy transfer, and therefore, the luminescence was recovered. On the basis of the restored luminescence, a turn-on optical immunosening system was developed. Under the optimal conditions, the linear range of detection was from 0.18 to 11.44 ng/mL for AFP (R = 0.99), with a detection limit as low as 0.16 ng/mL. The proposed method has also been used to monitor AFP in human serum samples. Therefore, further study based on the NaYF4:Yb,Tm/NaGdF4 core–shell nanoparticles-GNRs construction may open the way for a new class of NIR-LET biosensors with wide applications.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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