INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Wenshan Li,Qingqing Li,Huaiyue Xia,Wenjie Liu,Guoxing Jing,Jianna Yu,Wen Liu,Mengtong Lin,Yujuan Huang
Ochratoxin A (OTA) is a compound of concern due to its potential health effects on humans. Detecting OTA in food is crucial for safeguarding public health. In this study, we fabricated a multi-DNAzyme cascade reaction-mediated colorimetric aptasensors for OTA detection, integrating autocatalytic Mg 2+ -dependent DNAzyme cleavage (MNAzyme) and an entropy-driven circuit. In brief, the recognition between the aptamer and target OTA led to the release of DNA1. Subsequently, DNA1 hybridized with DNA2, generating an upstream MNAzyme that facilitated the production of a downstream MNAzyme. These MNAzymes possess similar substrate binding arms, enabling them to catalyze the same substrate. The catalytic efficiency of MNAzymes towards the substrates was enhanced due to the increasing concentration of MNAzymes. The cleavage products then triggered an entropy-driven cycle to generate a signal. Under optimal conditions, the sensing system exhibited low detection limits of 48.97 fM for OTA. Additionally, the proposed aptasensor was successfully applied to quantitatively analyze OTA in food samples. Thus, the multi-DNAzyme cascade reaction-mediated colorimetric aptasensors offer an adaptable platform for detecting traces of OTA contaminant in food.
研究领域:
食品安全检测
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Dandan Huang,Jiaming Chen,Li Ding,Longhua Guo,Palanisamy Kannan,Fang Luo,Bin Qiu,Zhenyu Lin
In this work, core-satellite assemblies and exonuclease assisted double amplification strategy is developed to produce surface-enhanced Raman scattering (SERS) biosensor towards ultrasensitive detection of biotoxin. In the presence of target molecules, the exonuclease III (Exo III) assisted efficient recycling amplification provides an excellent pathway for the fabrication of core-satellite SERS sensor. Briefly, the proposed strategy includes the following double amplifications: (i) Exo III induced target-related signal amplification; (ii) core-satellite assemblies assisted formation of SERS “hot-spots” induced signal amplification. To show the applicability of the suggested strategy, the detection of ochratoxin A (OTA), one of the most toxic and widely distributed biotoxin, is demonstrated as an example. The results show that the limit of detection (LOD) of OTA is 0.83 fg mL −1 (S/N = 3). On the basis of the DNA aptamer induced specific target recognition, hence our sensing strategy is easy to be expended to the ultrasensitive detection of other targets, e.g., DNAs, RNAs, and other molecules that have corresponding DNA aptamers.
研究领域:
分析化学
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毒理学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 3
·567
·
IF 4.2
Yayun Guo,Zhe Wang,Yi He,Huanhuan Gao,Hongmei Shi
Aspergillus carbonariusis known to produce the carcinogenic ochratoxin A (OTA) in grapes. The metabolism process before OTA biosynthesis influences the content and composition of the volatile compounds in grapes. In this study, a self-established method based on QuEChERS coupled with high-performance liquid chromatography–fluorescence detection (HPLC-FLD) was used to determine the OTA levels during a seven-day contamination period. The results showed that OTA was detected on the second day after contamination withA. carbonarius. Thus, the first day was considered as the critical sampling timepoint for analyzing the volatiles in grapes before OTA biosynthesis. Additionally, the volatile compounds in grapes were analyzed using headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) and dispersive liquid–liquid microextraction gas chromatography–mass spectrometry (DLLME-GC-MS). The corresponding data were evaluated via multivariate data analysis using projection methods, including PCA and OPLS-DA. The results indicated significant differences in the nine volatile compounds in grapes contaminated withA. carbonariusbefore OTA biosynthesis. The results of the Pearson correlation analysis showed positive correlations between ethyl acetate, styrene, 1-hexanol and OTA; (E)-2-hexenal and nerolic acid were negatively correlated with OTA. Overall, these findings provide a theoretical basis for the early prediction of OTA formation in grape and grape products using GC-MS technology.Keywords:volatile compounds;Ochratoxin A;QuEChERS-HPLC-FLD;HS-SPME-GC-MS;DLLME-GC-MS;OPLS-DA
研究领域:
食品科学
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真菌学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 3
·363-368
·
IF 1.248
Ma Lian,Xu Bin,Liu Leijing,Tian Wenjing
A label-free fluorescent aptasensor for specific and ultrasensitive monitoring ochratoxin A(OTA) was developed using the specific aptamer of OTA(OSA) as recognition element, an aggregation-induced emission(AIE) molecule(a 9,10-distyrylanthracene with two ammonium groups, DSAI) as a fluorescent probe, and graphene oxide(GO) as a quencher. In the absence of OTA, the AIE probe DSAI and OSA complex(DSAI/OSA) is adsorbed on the GO surface, and the fluorescence of DSAI will be quenched efficiently via the fluorescence resonance energy transfer(FRET) from DSAI to GO. Upon the OTA addition, a more stable complex(OSA-OTA) is formed and released from GO. Meanwhile, DSAI and OSA-OTA can form a new complex(DSAI/OSA-OTA), then the fluorescent signal of DSAI recovers gradually. Therefore, by introducing GO and DSAI, the fluorescence signal of DSAI can be easily turned from “off” to “on” after the addition of OTA, and the ultrasensitive detection of OTA by monitoring the change of the fluorescence signal of DSAI can be readily realized. The detection limit of the assay can reach 0.324 nmol/L with a linear detection range of 10—200 nmol/L. And the aptasensor exhibits high selectivity for OTA against other analogues. Moreover, it has been successfully applied for the detection of OTA in red wine samples.
研究领域:
分析化学
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食品安全
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毒理学
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生物化学
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产品关联
DOI
高影响因子
FOOD CONTROL
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2022
·
IF 6.652
Chengyuan Wu,Xiangchuan Wu,Fan Hou,Lina Wu,Guangxia Liu
An ultrasensitive electrochemical aptasensor was established based on metalloporphyrin metal organic frameworks ( [email protected] ) with peroxidase-like activity and exonuclease III-assisted recycling magnification for the detection of ochratoxin A (OTA). [email protected] showed well peroxidase-like activities due to Fe-tetrakis (4-carboxyphenyl) porphyrin (Fe-TCPP) as a heme-like ligand and in situ supported with palladium nanoparticle, which could be used for the label of ssDNA instead of nature enzyme. The designed exonuclease III-assisted recycling magnification further improve the sensitivity for the detection. Based on this, the proposed aptasensor showed a well linear relationship within the scope of 1 × 10 −5 ng mL −1 to 10 ng mL −1 and a low detection limit of 6.79 fg mL −1 . Additionally, the application in the real spiked oil samples confirmed the dependability and veracity of the aptasensor. Hence, the designed aptasensor has comprehensive application prospects for the detection of OTA in food .
研究领域:
食品安全
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分析化学
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生物技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaokun Wang,Hao Ding,Yinghui Sun,Yanling Ma,Guoqing Wang,Jiadong Chen,Jaebum Choo,Lingxin Chen
To address the need for highly sensitive and reliable detection of trace ochratoxin A (OTA) in food matrices, we developed a ratiometric fluorescent aptasensor by integrating CRISPR/Cas12a, hybridization chain reaction (HCR), and horseradish peroxidase (HRP)-induced inner filter effect (IFE). The mechanism involves OTA releasing an activator that initiates CRISPR/Cas12a trans-cleavage, blocking HCR assembly. This reduces HRP levels, limiting the conversion of o -phenylenediamine (OPD) to fluorescent 2,3-diaminophenazine (DAP) (emitting at 562 nm) while maintaining strong emission from 2-amino terephthalic acid (BDC-NH 2 ) at 426 nm. The F 426 /F 562 ratio serves as a “signal-on” indicator, enabling sensitive OTA detection over 0.1 pM to 10 nM, with a detection limit of 0.0417 pM. The method exhibits excellent reproducibility, with intra-day and inter-day relative standard deviations (RSDs) of 1.91 %–3.87 % and 1.79 %, respectively, along with recovery rates of 90.1 %–110.6 % in real samples. These advantages highlight its significant potential for CRISPR/Cas-based OTA detection.
研究领域:
食品安全
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiao Liang,Qiu-Lan Li,Ji-Tao Li,Wen-Bo Zhao,De-Zhi Yang,Ya-Ling Yang,Zi-Tao Zhong
Ochratoxin A (OTA) is recognized as a Group 2B carcinogen and poses significant dangers to human, making it crucial to regulate OTA levels in food products. Herein, we developed a colorimetric approach to analyze OTA utilizing the intrinsic catalytic oxidized activity of Fe 3 O 4 magnetic nanoparticles (MNPs). Our findings reveal that OTA shows a significant impact on the absorbance signal of the catalytic system, notably reducing the absorbance at 532 nm with H 2 O 2 and 4-aminoantipyrene (4-AP). This sensor does not require elaborate aptamer or antibody, omitting the cumbersome reaction and the loss during synthesis, thus simply realizing OTA detection. The signal correlates linearly with the OTA concentrations and achieves satisfied sensitivity. The practical application has been verified utilizing beer and rice samples, resulting in recoveries ranging from 91.0 % to 106.2 %. This accuracy and reliability verified the method feasibility for monitoring OTA levels in food products.
研究领域:
食品科学
·
分析化学
·
毒理学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 14
·3683-3693
·
IF 4.157
Zhang Lili,Zhang Ziyu,Tian Yu,Cui Meihui,Huang Beibei,Luo Tao,Zhang Shufang,Wang Hanjie
How to achieve simultaneous and rapid detection of various mycotoxins in food has important practical significance in the field of food processing and safety. In this paper, a smartphone immunoassay system based on hydrogel microspheres has been constructed to quickly detect two mycotoxins at the same time. The rapid detection system was reflected in the following three processes: (1) rapid separation of free matter after direct competition reaction based on hydrogel solid-phase carrier particles; (2) rapid detection process based on efficient catalytic function of enzymes; (3) fast capture and analysis of images based on smartphone software. Ochratoxin A (OTA) and zearalenone (ZEN) are secondary toxic metabolites of fungi that can contaminate a wide range of foods and feeds. OTA and ZEN were used as detection model molecules to verify the feasibility of the intelligent rapid detection system. The entire detection process was within 30 min, and the results were analyzed in only 10 s. Detection limits of mycotoxins OTA and ZEN are 0.7711 ng L −1 and 1.0391 ng L −1 . The recoveries of both mycotoxins ranged from 76.72 to 122.05%. This study provides a universal rapid detection method for on-site application of large-scale food security testing. Graphical abstract
Schematic diagram of the construction of the smartphone detection system: The system is divided into three parts: detection, image capture and analysis, and result.
研究领域:
食品科学
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分析化学
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生物技术
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Ailing Lei,Mingda Li,Siqi Zhuo,Qianjin Li,Jianlin Li
Ochratoxin A (OTA) with high toxicity is widely present in agricultural products, posing an enormous threat to human and animal health. Thus, simple, rapid, sensitive and selective detection of OTA is highly necessary. Herein, we have developed a new label-free fluorescence and naked-eye detection platform for ochratoxin A based on the interaction between OTA-antiparallel G-quadruplex(G4)/crystal violet (CV). CV and OTA aptamer could form a G4 structure with high fluorescence intensity. When OTA was present in the G4/CV mixture, it replaced CV and bound to the OTA aptamer, and then the fluorescence of the mixture remarkedly quenched. The interaction among OTA and G4/CV had been revealed by fluorescence, ultraviolet and circular dichroism (CD) spectra. The detection process could be performed by naked-eye and could be completed within 10 min. The established method showed 0.1–1000 ng/mL linear detection range with 0.07 ng/mL limit of detection (LOD). The recovery rates in cereal samples were in the range of 76–105%. Compared with the classical ELISA, the recovery rates and the detected concentration of OTA in the naturally contaminated cereal samples were consistent. The new method can provide a simple, rapid and cost-effective, label-free and naked-eye detection of OTA with high specificity, good repeatability and high precision.
研究领域:
分析化学
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毒理学
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食品安全
·
生物化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhixue Yu,Yue He,Hui Wang,Dongxia Pan,Ruipeng Chen,Xiangfang Tang,Junhu Yao,Benhai Xiong
Ochratoxin A (OTA) is a highly toxic mycotoxin with nepHrotoxicity, hepatotoxicity, and carcinogenicity. It poses a long-term threat to the health of humans and animals. Although screen printed electrodes (SPE) with aptamer functionalization provide promising detection capabilities, their applications are limited by inherent weak electrochemical signals, prompting the need for signal amplification strategies. In this study, tetrahedral DNA clusters (TDCs) were prepared as aptamer probes for OTA, and an aptamer-based electrochemical sensing method for OTA was established for signal amplification. Multiple single-stranded DNA (ssDNA) were designed to self-assemble into tetrahedral DNA nanostructure (TDN) through base complementarity, with the OTA aptamer attached to one vertex. Additional ssDNA strands were further utilized to extend TDNs at the three remaining vertices, forming a DNA tetrahedral cluster. The method had a linear range of 1 pg/mL to 100 ng/mL (LOD = 1 pg/mL) for detecting OTA, with excellent specificity and satisfactory recovery rate in feed samples
研究领域:
生物传感器
·
纳米技术
·
生物化学
·
分析化学
·
分子生物学
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食品安全
·
毒素检测
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Xiaoya Bi,Lijun Luo,Libo Li,Xiaohong Liu,Bainian Chen,Tianyan You
A new fluorescence aptasensor for Ochratoxin A (OTA) analysis in corn and barley flour was developed owing to the favourable quenching function of cobalt oxyhydroxide (CoOOH) nanosheets . The first combination of graphitic carbon nitride quantum dots (g-CNQDs) and CoOOH nanosheets as efficient energy donor-acceptor pair was reported, and the quenching mechanism was proved by investigating the fluorescence lifetime of g-CNQDs. The aptamer-modified g-CNQDs (g-CNQDs-apt) were adsorbed onto CoOOH nanosheets surface by van der Waals force . Consequently, the Förster resonance energy transfer (FRET) from g-CNQDs-apt to CoOOH nanosheets was initiated, leading to quenched fluorescence. With the addition of OTA, the linear aptamer specifically bound with OTA to form G-quadruplex, which had relatively weak interaction with the CoOOH nanosheets and separated from the nanosheets surface. Thus, the FRET process between g-CNQDs-apt and CoOOH nanosheets was hindered, leading to the fluorescence of g-CNQDs-apt recovered clearly. The developed aptasensor exhibited acceptable detection limit with 0.5 nM and desirable linear relationship from 1 nM to 140 nM. Meanwhile, the aptasensor possessed multiple advantages, including easy operation, rapid detection and high selectivity . Moreover, the aptamer sensing platform was favorably applied for OTA determination in cereal (barley and corn flour), in which the recoveries varied from 94.5% to 101% with the relative standard deviation under 2.24%.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Meiling Tian,Wenyue Xie,Ting Zhang,Yang Liu,Zhisong Lu,Chang Ming Li,Yingshuai Liu
This work reports a low-cost, fast and highly sensitive lateral flow immunoassay (LFIA) platform with Prussian blue nanoparticle (PBNP) as a new reporter, which efficiently mediates an on-demand cascade signal amplification by catalyzing oxidation of chromogenic reagent 3,3′,5,5′-tetramethylbenzidine (TMB). In this case, PBNPs are accumulated on the test line through the specific immune interaction, resulting in a blue-colored line. The colorimetric signal can be further amplified via the peroxidase mimic PBNP-catalyzed TMB oxidation to produce an insoluble blue-violet precipitate. Under an optimal condition, a limit of detection (LOD) at 10 pg mL −1 is achieved for rabbit IgG (RIgG) detection, which is 2–3 orders of magnitude better than the conventional LFIA strip using colloidal gold nanoparticle as a label. A macromolecular RIgG and a small molecular mycotoxin ochratoxin A (OTA) are also successfully analyzed in mimicking samples, demonstrating its good practicability of the proposed platform in a variety of fields.
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）