ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 40
·91997-92006
·
IF 5.8
Ran
Chunmei,Liu Yubin,Li Ke,Wang Chenye,Pu Jian,Sun Hongwen,Wang Lei
Although the combined effect of organic ligands and heavy metals in the environment on plants have been frequently reported, their complexed interaction in plants and the physiological effects remain to be revealed. Metal complexing agent benzotriazole (BTR) has extensive environmental pollution. In this study, root-splitting experiments were designed to identify the in vivo and in vitro effects of BTR on the accumulation and translocation of Cu in rice ( Oryza sativa L . ), and the concentrations and translocation factor (TF) of Cu and BTR in different parts of rice were measured. In the in vitro interaction treatments, low BTR concentrations enhanced Cu uptake and lateral transport in rice, while higher levels of BTR’s exposure (i.e., ≥ 100 μM) resulted in opposite effects. Differently, significant increase in the lateral transport of Cu and vertical translocation of BTR in rice presented in the in vivo interaction treatments. TF of Cu from root A to root B (TF RA-RB ) increased from 0.05 to 0.272 with the BTR concentration increasing from 0 to 100 μM, and higher TF of BTR from root to shoot (TF R-S ), ranging from 1.00 to 1.75, compared with single BTR exposure treatments was observed. The phytotoxicity of BTR expressed by the catalase activity was significantly alleviated by the in vivo accumulated Cu in rice.
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高影响因子
Antioxidants
·
2023
·Vol. 12
·期 11
·1917
·
IF 7
Yutan Chu,Qiongli Bao,Yan Li,Hongyu Sun,Zewei Liu,Jiahao Shi,Yizong Huang
Antimony (Sb) is a hazardous metal element that is potentially toxic and carcinogenic. Melatonin (MT) is an indole compound with antioxidant properties that plays an essential role in plant growth and alleviates heavy metal stresses. Nevertheless, little is known about the effects and mechanisms of exogenous MT action on rice under Sb stress. The aim of this experiment was to explore the mechanism of MT reducing Sb toxicity in rice via hydroponics. The results showed that Sb stress significantly inhibited the growth of rice, including biomass, root parameters, and root viability. Exogenous MT obviously alleviated the inhibition of Sb stress on seedling growth and increased biomass, root parameters, and root viability by 15–55%. MT significantly reduced the total Sb content in rice and the subcellular Sb contents in roots by nearly 20–40% and 12.3–54.2% under Sb stress, respectively. MT significantly decreased the contents of malondialdehyde (MDA, by nearly 50%), ROS (H2O2and O2·−, by nearly 20–30%), and RNS (NO and ONOO−) in roots under Sb stress, thus reducing oxidative stress and cell membrane damage. Furthermore, MT reversed Sb-induced phytotoxicity by increasing the activities of antioxidant enzymes (SOD, POD, CAT, and APX) by nearly 15% to 50% and by regulating the AsA–GSH cycle. In conclusion, this study demonstrates the potential of MT to maintain redox homeostasis and reduce Sb toxicity in rice cells, decreasing the content of Sb in rice and thereby alleviating the inhibition of Sb on rice growth. The results provided a feasible strategy for mitigating Sb toxicity in rice.Keywords:antimony (Sb);melatonin (MT);rice;antioxidant;AsA–GSH cycle
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CHEMOSPHERE
·
2023
·
IF 8.8
Peicheng Huang,Weichun Yang,Qingzhu Li,Qi Liao,Mengying Si,Meiqing Shi,Zhihui Yang
In this study, a novel slightly-soluble selenium (Se) fertilizer (SSF) was successfully applied to address the problems of Cd pollution in paddy soil and rice, and Se deficiency in human beings. The pot and field experiments showed that Cd content in the rice grains was reduced by 48.4%–82.89% and Se content was increased nearly by 30-fold comparing the control group. The application of SSF increased the soil pH and significantly reduced the DGT-extracted Cd in the soil. Moreover, DCB-extractable Fe content on the surface of roots was prompt by SSF, which formed a physical barrier, namely iron plaque (IP), to inhibit Cd translocation to the above-ground tissues of the rice plants. The Cd content in the IP was also decreased before the filling period, possibly contributing to the reduction in major Cd accumulation in the rice grains. In addition, the continuous Se increase and Cd reduction in the IP by the SSF gradually exceeded that of water-soluble Se during the three periods of rice plant growth. This suggests that SSF has high potential to be an effective Se fertilizer for inhibiting Cd uptake and enriching Se in rice.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yi Hu,Nuzahat Habibul,Yan-Yun Hu,Fan-Li Meng,Guo-Ping Sheng
Ciprofloxacin (CIP), a widely used fluoroquinolone antibiotic, is frequently detected in aqueous environments, and could be assimilated by vegetable plants to possess potential threats to human and animal health through food chains. However, plant uptake of CIP in different chemical speciation has still far from clear now. Thus, the toxicity and uptake of CIP by rice plants were investigated under different solution pH, owing to its contribution to different chemical speciation of CIP. Results display that high pH-driven changes of CIP from cation (CIP + ) to anion (CIP − ) decreased its adsorption and uptake by excised roots and intact plants, respectively. However, CIP concentrations in roots, stems and leaves all exhibited no significant differences with increasing solution pH. Moreover, six intermediates of CIP were detected and two possible transformation pathways were proposed in rice plants, including firstly oxidation and following consecutive cleavage of piperazine ring. After accumulated in plant tissues, CIP significantly inhibited the plant growth, decreased the photosynthetic pigments contents and enhanced the antioxidant enzyme activities in a concentration-dependent manner. Besides, high pH exacerbated the growth inhibition and changed the oxidative damage responses of rice plants to CIP. These findings indicate that the uptake and toxicity of CIP in rice plants were influenced by solution pH-driven changes of its chemical speciation.
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ENVIRONMENTAL AND EXPERIMENTAL BOTANY
·
2020
·
IF 4.027
Shangguang Du,Meng Cui,Yali Cai,Ahui Xue,Yingbin Hao,Xueyong Huang,Lihua Liu,Liping Luo
Chilling stress is a major production threat for rice ( Oryza sativa L.). The mechanism of chilling response has not been unveiled up to now, especially at the metabolomic level. In this study, two Asian rice cultivars, Qiutianxiaoting (chilling-tolerant variety) and 93−11 (chilling-susceptible variety), were used to analyze the metabolic changes when exposed to low temperature and recovered by extractive electrospray ionization mass spectrometry (EESI-MS). Physiological and metabolic analysis combining with bioinformatics were performed to reveal cold resistance mechanism of these two varieties. Physiological analysis showed that only Qiutianxiaoting survived after 36 h chilling treatment, and the growth indexes of Qiutianxiaoting took on a decreasing trend, but these indexes of 93−11 appeared a trend of increase under cold treatment; moreover, the levels of antioxidant activities (SOD, POD and CAT) were higher in Qiutianxiaoting than 93−11, especially at recovery stage. PCA and OPLS-DA analysis all showed that Qiutianxiaoting, not 93−11, could be separated with each other under chilling stress, which indicated that these two subspecies of rice had different mechanisms to resist cold temperature. The results of metabolic analysis indicated that low temperature activated phenylpropanoid biosynthesis and flavone and flavonol biosynthesis in 93−11, while it induced the expression of methyl jasmonate biosynthesis-related genes in Qiutianxiaoting, which might mitigate chilling damage and effectively make it more cold-resistant than 93−11. This study would shed some lights on the cold resistance mechanism of these two rice cultivars in terms of physiological and metabolic analysis.
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Food Science & Nutrition
·
2020
·Vol. 8
·期 6
·2882-2894
·
IF 1.797
Changyuan Wang,Yuchao Feng,Shu Zhang,Tianxin Fu,Yanan Sheng,Yiwei Zhang,Yingjun Jiang,Miao Yu,Liyuan Zhang,Dongjie Zhang
Metabolomic studies were carried out using gas chromatography and mass spectrometry (GC-MS) on Daohuaxiang variety rice ( Oryza sativa L.) from the Wuchang Geographical Indication Rice Protection Area in Heilongjiang Province, to investigate the effects of storage on brown rice metabolism. The data were subjected to principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and cluster analysis using software such as SIMCA. Analysis of the samples led to the identification of a total of 160 metabolites. No significant differences were found in the amount of metabolites before and after storage. A total of 31 differential metabolites were screened, and the changes in metabolite content showed a “reverse change” overall. Storage significantly changed the content of various metabolites in rice, with fatty acids impacted most significantly. Metabolic pathway analysis revealed that fatty acid biosynthesis is a key metabolic pathway in rice storage. The degradation of brown rice quality caused by storage is closely related to the composition and content of its metabolites, and that change in lipid content significantly affects brown rice quality during storage.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Dong Zhang,Luyao Zhao,Wenjuan Wang,Qian Wang,Jianlei Liu,Yuanyuan Wang,Hui Liu,Bo Shang,Xiaoliang Duan,Hui Sun
In the current study, the changes of non-starch and starch lipids in two rice varieties during storage were investigated based on untargeted lipidomic approach. A total of 395 non-starch lipids and 13 starch lipids were identified in two rice varieties. 171 lipids in Nanjing and 6 lipids in Jianzhen were significantly different in stored rice compared to those in the fresh ones. Bioinformatics analysis on these significantly different lipids revealed that the two rice varieties possessed different lipids metabolic pathways during storage. Our study provides insights into the mechanism of the changes in non-starch and starch lipids in rice during storage.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Liwen Qiang,Nan Zhao,Kaizhen Liao,Xichao Sun,Qiang Wang,Hangbiao Jin
Engineered nanoparticles (ENPs) can resist heavy metal toxicity in plants, but their coexposure still exhibits toxicity to plants compared to plants without exposure to ENPs and heavy metals. There have been few studies on the toxic mechanism of nano TiO 2 -heavy metal coexposure and the effect mechanism of nano TiO 2 in plants. Thus, transcriptomics and metabolomics were used to study the toxic mechanism of rutile nano TiO 2 or TiO 2 -Cd (rutile nano TiO 2 and CdCl 2 mixture) on rice ( Oryza sativa L.). After 40 days of exposure, the plant height and root dry weight of rice were significantly decreased in the nano TiO 2 -Cd group compared to the blank group (nano TiO 2 and CdCl 2 free). After Cd treatment, 423 differentially expressed genes (DEGs) and 16 differential metabolites were identified. Nano TiO 2 exposure induced significant regulation of 299 DEGs and 6 metabolites. After nano TiO 2 -Cd coexposure, 1660 DEGs and 181 differential metabolites were identified. Notably, the EDGs (e.g., chalcone isomerase and hydroxycinnamoyl transferase) and differential metabolites (e.g., chrysin and galangin) demonstrated the disruption of flavonoid biosynthesis in Cd-treated rice. After rice was exposed to nano TiO 2 , the DEGs were related to ribosome, whereas the differential metabolites were associated with pyruvate metabolism and valine, leucine , and isoleucine biosynthesis. Furthermore, 14 DEGs (e.g., asparaginyl-tRNA synthetase and methionyl-tRNA formyltransferase) involved in aminoacyl-tRNA biosynthetic pathways were significantly upregulated in rice treated with nano TiO 2 -Cd, in line with the changes in related metabolites (e.g., L -asparagine and 10-formyltetrahydrofolate). Our results show that it is necessary to pay close attention to the toxicity of nano TiO 2 -Cd coexposure in paddy ecosystems and use ENPs with caution to combat the phytotoxicity of heavy metals.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zihang Cheng,Nannan Li,Zhining Chen,Kaikai Li,Dongling Qiao,Siming Zhao,Binjia Zhang
High-fat diet is a risk factor for many chronic diseases, whose symptoms are probably regulated by ingesting food ingredients such as resistant starch. For cooked rice stored in cold-chain, the starch component can retrograde to generate ordered structures (helices and crystallites) and become resistant. However, the role of retrograded starch in managing hyperlipidemia symptoms is insufficiently understood. Here, compared to the normal high-fat diet, ingesting retrograded starch reduced the triglyceride and low-density lipoprotein cholesterol levels of high-fat diet mice by 17.69% and 41.33%, respectively. This relieved hyperlipidemia could be linked to the changes in intestinal bacteria. Retrograded starch intervention increased the relative abundance of Bacteroides (2.30 times higher), which produces propionic acid (increased by 8.26%). Meanwhile, Bacteroides were positively correlated with butyric acid (increased by 98.4%) with strong anti-inflammatory functions. Hence, retrograded starch intervention may regulate the body's health by altering intestinal bacteria.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Mingwei Deng,Shengwang Wang,Huagang Huang,Daihua Ye,Xizhou Zhang,Yongdong Wang,Zicheng Zheng,Tao Liu,Tingxuan Li,Haiying Yu
Hydrogen peroxide (H 2 O 2 ) is a vital signaling molecule in response to cadmium (Cd) stress in plants. However, the role of H 2 O 2 on Cd accumulation in root of different Cd-accumulating rice lines remains unclear. Exogenous H 2 O 2 and 4-hydroxy-TEMPO (H 2 O 2 scavenger) were applied to investigate the physiological and molecular mechanisms of H 2 O 2 on Cd accumulation in the root of a high Cd-accumulating rice line Lu527–8 through hydroponic experiments. Interestingly, it was found Cd concentration in the root of Lu527–8 increased significantly when exposed to exogenous H 2 O 2 , while reduced significantly when exposed to 4-hydroxy-TEMPO under Cd stress, proving the role of H 2 O 2 in regulating Cd accumulation in Lu527–8. Lu527–8 showed more Cd and H 2 O 2 accumulation in the roots, along with more Cd accumulation in cell wall and soluble fraction, than the normal rice line Lu527–4. In particular, more pectin accumulation, especially low demethylated pectin, was observed in the root of Lu527–8 when exposed to exogenous H 2 O 2 under Cd stress, resulting in more negative functional groups with greater capacity to binding Cd in the root cell wall of Lu527–8. It indicated that H 2 O 2 -induced cell wall modification and vacuolar compartmentalization contributes greatly to more Cd accumulation in the root of the high Cd-accumulating rice line.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jie Chen,Qin Liu,Longyu Yuan,Wenzhong Shen,Qingxing Shi,Guojun Qi,Ting Chen,Zhenfei Zhang
The brown planthopper (BPH) is the most serious pest causing yield losses in rice. MicroRNAs (miRNAs) are emerging as key modulators of plant–pest interactions. In the study, we found that osa-miR162a is induced in response to BPH attack in the seedling stage and tunes rice resistance to the BPH via the α-linolenic acid metabolism pathway as indicated by gas chromatography/liquid chromatography-mass spectrometry analysis. Overexpression of osa-miR162a inhibited the development and growth of the BPH and simultaneously reduced the release of 3-hexenal and 3-hexen-1-ol to block host recognition in the BPH. Moreover, knockdown of OsDCL1, which is targeted by osa-miR162a, inhibited α-linolenic acid metabolism to enhance the resistance to the BPH, which was similar to that in miR162a-overexpressing plants. Our study revealed a novel defense mechanism mediated by plant miRNAs developed during the long-term evolution of plant–host interaction, provided new ideas for the identification of rice resistance resources, and promoted a better understanding of pest control.
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高影响因子
Food Analytical Methods
·
2016
·Vol. 9
·期 12
·3501-3508
·
IF 2.167
Shao Yufang,Chen Ying,Liu Jian,Wu Pingping,Ying Yibin,Xie Jing
High-resolution inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine potential toxic element pollution in rice and paddy soil in a lead-acid battery production area. The contamination levels of chromium (Cr), cadmium (Cd), and lead (Pb) in rice and soil at three sampling areas were determined, and the uptake mechanism of Cr, Cd, and Pb from soil to rice grain was preliminarily analyzed. The results show that the range of potential toxic elements in rice concentrations is 3.914–11.788, 0.057–1.215, and 0.187–1.585 mg kg −1 for Cr, Cd, and Pb, respectively. For the paddy soils, Cd concentration is 0.610 mg kg −1 , which exceeds 0.300 mg kg −1 , a value recommended by Chinese Environmental Quality. Bioaccumulation factor (BAF, an index of the ability of plant to absorb a particular metal with respect to its concentration in the soil substrate) values of Cr, Cd, and Pb were less than 1 in all rice samples, suggesting that the bioavailability of these metals was relatively low in rice. The correlations between BAF values with organic matter (OM) and pH were calculated to find the mobility and availability of potential toxic elements in soil. At last, the estimated daily intake (EDI) dose was determined to evaluate the health risk to local population via consumption of rice contaminated with Cr, Cd, and Pb. This study indicated that the accumulation and translocation of potential toxic elements from soil to rice could cause the health risk for the local population.
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危险品化学品经营许可证（带存储）