ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Yongqiang Du,Xiao Wang,Dongqi Lian,Yingliang Liu,Lei Zhang,Shengang Xu,Shaokui Cao
The modification of perovskite intergranular perovskite/perovskite interface plays a critical role for power conversion efficiency (PCE) and stability of perovskite solar cells (PSCs). In this work, polyamidoamine (PAMAM) dendrimers are utilized as the dendritic crystallization framework templating the perovskite-crystallizing process. The interactions at the perovskite intergranular interface are considerably strengthened at an ambient environment with dendritic PAMAMs crosslinking the perovskite grains. Consequently, the perovskite morphology is remarkably improved by suppressing the grain/grain-aggregate boundaries for the pinhole removal, which produces a compact, uniform and non-pinhole perovskite film. Finally, the strengthened interfacial interactions dramatically enhance the PCE value of unencapsulated PSCs about 42.6% at an ambient condition. Besides, the unencapsulated PAMAM-modified device can keep 73% of initial PCE value in 400 h while the control device decays to 5% of initial PCE value in 50 h. These results reveal that dendritic polymers might remarkably improve the PCE value and the stability of PSCs. This work provides a new molecular design guideline to effectively regulate the perovskite intergranular interfacial interactions.
研究领域:
材料科学
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能源科学
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化学工程
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 6
·1266-1276
·
IF 5.344
Lingzhu Yu,Bin Luo,Zhiyu Li,Jia He,Fang Lan,Yao Wu
Selective capture of mono-, multi- or global phosphopeptides is significant for the in-depth study of protein kinase or phosphatase signal transduction pathways. However, this study is largely restricted because of the lack of versatile affinity materials with high enrichment capacity and tunable selectivity. Here, we prepared a smart nanoprobe for the selective enrichment of mono-, multi- or global phosphopeptides by introducing polyamidoamine dendrimer (PAMAM)-grafted poly(methacrylic acid) (PMAA) brushes to modified magnetic composite nanospheres (denoted as Fe3O4@PDA@PMAA@PAMAM). The practicability of the prepared nanospheres for phosphopeptide enrichment was investigated by using model proteins (α-casein and β-casein) and complex biological samples (nonfat milk and human saliva). The extremely abundant amine groups of PAMAM–PMAA brushes and superparamagnetism of the Fe3O4 core endowed the composite nanospheres with high detection sensitivity (1 fmol μL−1), excellent selectivity (1 : 500 molar ratios of β-casein/BSA) and high recyclability (five cycles) towards phosphopeptides. Additionally, based on the tunable bonding ability of the nanospheres towards phosphopeptides with different phosphorylation sites, selective enrichment of mono-, multi- or global phosphopeptides was realized by modulating buffer polarity and acidity, making them ideal new nanoprobes for comprehensive phosphoproteome analysis.
研究领域:
生物化学
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纳米医学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Siqin He,Bin Xu,Ying Liu,Xiaoshuang Yin,Wenzhong Yang,Yun Chen
Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe 3 O 4 -SiO 2 composite particles , respectively. Using Fe 3 O 4 as a magnetic center and SiO 2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO 2 successfully, while laccase was immobilized to the surface of Fe 3 O 4 -SiO 2 -PAMAM x by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
CHEMISTRY AND PHYSICS OF LIPIDS
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2018
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IF 2.766
Yingying Feng,Haimei Hu,Shuanghong liang,Dan Wang
As an efficient gene delivery, non-viral vectors should have high transfection efficiency, excellent endosomal escape, low cytotoxicity, and the ability to rapidly release the gene into the cytoplasm.Cationic liposome have been widely used as efficient gene carriers, but the cytotoxicity, rapid degradation and low cellular uptake are major drawback impeding its further appolication. Herein, with double lauric acid as hydrophobic chains, tartaric acid as skeleton, 0 generation PAMAM modified with lysine as hydrophilic head, a new type cationic peptide lipid was synthetised. The alkyl chain promote lipid across cell membranes and with membrane fusion , 0 generation PAMAM modified with lysine hydrophilic end amino can contain a large number of protons which can change into ammonium and combine with the DNA negatively charge phosphate groups . It is expected that this carrier has low toxicity, high transfection efficiency and targeting property. By adjusting the cationic liposome/gene weight ratio, the transfection system was optimized to improved gene transfection efficiency, reduce cytotoxicity, and increase property and stability, etc.
研究领域:
生物技术
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药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
·
IF 3.807
Jia Ding,Wenjing Jiang,Yunlei Zhou,Huanshun Yin,Shiyun Ai
As a significant potential biomarker for early disease diagnostics, the detection of 5-hydroxymethylcytosine (5hmC) has garnered widespread attention. In this work, a simple and sensitive electrochemiluminescence (ECL) was developed for 5hmC detection. After substrate electrode preparation, anti-5hmC antibody was captured on electrode surface by the formation of amide bond. Then, through immunoreaction, 5hmC was specifically recognized and captured. Utilizing the crosslink effect of Zr 4+ with phosphoric acid recognition, aminomethyl phosphate was modified on electrode to further capture PAMAM-silver nanoclusters/Nitrogen-doped graphene nanocomposite (PAMAM-Ag-NG), where this nanocomposite was employed as ECL signal probe. The designed immunosensor showed a linear concentration range from 10 pM to 30 nM with a low detection limit of 2.47 pM (S/N = 3). This method presented good selectivity and reproducibility. To prove the applicability, this method was further used to study the influence of phytohormone and avian leukemia virus on 5hmC content in maize seedling leaves and chicken fibroblast cells, respectively.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 9
·3273-3279
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IF 3.269
Chen-Tao Xu,Guang Chen,Xuan Nie,Long-Hai Wang,Sheng-Gang Ding,Ye-Zi You
Poly(amidoamine)s (PAMAMs) of high-generations (G > 5) exhibit high efficacy in gene transfection, but their high cost and severe cytotoxicity limit their use in biomedicine. In contrast, PAMAMs of low-generations (G < 3) are less toxic and easy to synthesize, but exhibit low transfection efficacy. In this report, we design a novel amphiphilic dendrimer by conjugating reactive oxygen species (ROS)-responsive poly(propylenesulfide) (PPS) to a low generation PAMAM dendrimer (G = 2, Mw = 3500 Da) via disulfide bonds. This amphiphilic conjugate can self-assemble into stable nanomicelles. The results show that the obtained nanomicelles have a high zeta potential, which can condense DNA tightly. Moreover, the DNA/nanomicelle polyplexes can be fully dissociated by intracellular ROS and easily release the entrapped DNA. As gene vectors, the nanomicelles exhibited high transfection efficacy and fairly low cytotoxicity in vitro.
研究领域:
生物材料学
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分子生物学
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药剂学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2016
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IF 3.994
Kang Chen,Qing Chen,Kuanglei Wang,Jia Zhu,Weinan Li,Wenpan Li,Lipeng Qiu,Guannan Guan,Mingxi Qiao,Xiuli Zhao,Haiyang Hu,Dawei Chen
The present report describes the synthesis of a hydroxyl terminal PAMAM dendrimer (PAMAM-OH) derivative (PAMSPF). The hydroxyls of PAMAM-OH were attached to S-Methyl- l -cysteine (SMLC) via an acid-labile ester bond, named as β-thiopropionate bond, followed by modification with folic acid (FA) through a polyethylene glycol (PEG) linker. The degrees of attachment of SMLC and FA to the PAMAM-OH backbone were 83.9% and 12.8%, respectively. PAMSPF could condense DNA to form spherical nanoparticles with particle sizes of ∼200 nm and remain stable in the presence of heparin and nuclease . The β-thiopropionate bond in PAMSPF was hydrolyzed completely and the DNA release rate was 95.8 ± 3.3% after incubation under mildly acidic conditions at 37 °C for 3 h. PAMSPF/DNA was less cytotoxic to KB and HepG2 cells and exhibited a higher gene transfection efficiency than native PAMAM/DNA. The uptake assays showed that PAMSPF/DNA entered KB cells within 0.5 h through folate receptor-mediated endocytosis and escaped from endosomes within 2 h. In addition, PAMSPF/DNA displayed long circulation time along with excellent targeting of tumor sites in vivo. These findings demonstrate that PAMSPF is an excellent carrier for safe and effective gene delivery.
研究领域:
生物化学
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分子生物学
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药剂学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Wenjing Jiang,Linni Wu,Junling Duan,Huanshun Yin,Shiyun Ai
An ultrasensitive sandwiched electrochemiluminescence (ECL) immunosensor was developed for 5-hydroxymethylcytosine (5hmC) detection in genomic DNA by using Fe 3 O 4 @SiO 2 core–shell magnetic nanomaterial as a immobilization matrix for anti-5hmC antibody, PAMAM conjugated avidin and Ru(bpy) 2 (phen-5-NH 2 )(PF 6 ) 2 as signal amplification unit. Importantly, Fe 3 O 4 @SiO 2 nanoparticles were verified to not only possess enormous surface for loading antibody by amido link, but also exhibit excellent bioactivity . With the dual signal amplification strategy, the ECL immunosensor showed wide detection range from 0.1 to 30 nM with low detection limit of 0.047 nM (S/N = 3). Based on the specific immunoreaction, the developed method also illustrated excellent detection selectivity . The fabricated immunosensor was also applied to detect the 5hmC in genomic DNA of cancer tissue, which indicated that the immunosensor possess potential applications in clinical detection.
研究领域:
分析化学
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生物化学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2021
·
IF 3.512
Hong Cui,Shuaishuai Cui,Qiuju Tian,Siyuan Zhang,Mingxiu Wang,Ping Zhang,Yunfeng Liu,Jialing Zhang,Xiangjun Li
A nanocomposite was formed by combining graphene oxide (GO) with chromium-centered metal–organic framework (Cr-MOF) nanoparticles regulated by the dendrimer polyamidoamine (PAMAM). PAMAM can successfully regulate the synthesis of Cr-MOF; in doing so, the size of Cr-MOF is reduced, its original morphology is maintained, and it has good crystallinity. A simple ultrasonication method was used to make the Cr-MOF/GO hybrid nanocomposite. Various characterization methods confirmed the successful synthesis of PAMAM/Cr-MOF/GO nanocomposites. The PAMAM/Cr-MOF/ERGO modified electrode could be used with cyclic voltammetry (CV) and differential pulse voltammetry (DPV) to study the electrochemical behaviors of 1-hydroxypyrene (1-OHPyr). The results indicated that the constructed PAMAM/Cr-MOF/ERGO electrochemical sensor had a significantly enhanced electrocatalytic effect on the electrochemical reduction of 1-OHPyr compared with the sensors with no PAMAM and the ERGO sensor, which could be ascribed to the synergetic effect from the high porosity of Cr-MOF and the high conductivity of ERGO, as well as the further electron transport action of the nanocomposite. Under the optimal conditions, the reduction peak current and concentration of 1-OHPyr showed a good linear relationship in the range of 0.1–1.0 and 1.0–6.0 μM, and the detection limit of 1-OHPyr was calculated to be 0.075 μM. Moreover, the PAMAM/Cr-MOF/ERGO electrochemical sensor constructed in this paper can be expected to provide some instructions for the construction of electrochemical sensing platforms and wider potential applications.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Science China-Technological Sciences
·
2023
·Vol. 66
·期 4
·1129-1140
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IF 4.6
Zhou LiPing,Li JiaWei,Yu Bing,Zhang Jun,Hu Hao,Cong HaiLin,Shen YouQing
Poly(amidoamine) (PAMAM) dendrimers are widely studied as drug vectors due to their particular structure and excellent properties. Drug molecules can be loaded in the internal cavity of dendrimers or adsorbed on the surface. In this paper, the interaction force and spatial configuration between PAMAM and several typical chemotherapy drugs (doxorubicin (DOX), paclitaxel (TAX), hydroxycamptothecin (HCPT)) is studied by molecular dynamics (MD) simulations. Several essential parameters of dendrimers as drug carriers are analyzed by combining experiments and MD simulations, including particle size, drug loading, drug release, and biocompatibility. The simulation of dendrimer@drug complexes demonstrates that many cavities are semi-open, and most drug molecules are not completely wrapped. The internal structure of PAMAM dendrimers became looser with more extended nuclei, which increased the non-covalent interactions between PAMAM dendrimers and drug molecules. The umbrella sampling simulations reveal that the change of binding energies between dendrimers and drug molecules is responsible for the variation in drug release rate. This study provides valuable enlightenment information for the drug loading/release of PAMAM dendrimers.
研究领域:
药学
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纳米技术
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分子生物学
·
生物物理学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Ziyin Xiang,Liangbo Xu,Xinyue Wei,Da Xu,Haoyu Xiong,Shengfu Chen
Functional oligopeptides derived from natural proteins are often used as targeting groups or therapeutic drugs but always suffer from a dramatic bioaffinity decrease due to the loss of conformational restriction in proteins, which increases their entropy state. It is worth noting that the linear Cys-Arg-Gly-Asp-Ser (CRGDS) oligopeptide can spontaneously restore its natural conformation by anchoring one terminus and forming hydrogen bonds on another terminus, on protein-like zwitterionic fifth-generation polyamide-amine (G5 PAMAM) nanocarriers, thus exhibiting high bioaffinity. However, the different physicochemical properties of nanocarriers have an impact on the bioaffinity recovery of linear CRGDS. In this work, it is found that the bioaffinity decreases with the reduction of generation of PAMAM and zwitterionic G3 PAMAM is a turning point for bioaffinity recovery via a series of in vitro experiments. This indicates that the spontaneous restoration of the native conformation of the RGD segment requires proper surface group density and structural rigidity of the zwitterionic nanocarrier for reducing the entropic energy of CRGDS peptides before binding. This approach paves a way for functional reproduction of protein molecules using low-cost synthetic polymers for extensive biomedical applications.
研究领域:
纳米技术
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生物医学工程
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药学
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分子生物学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 21
·3970-3981
·
IF 4.543
Mengjun Zhang,Shasha Jing,Jie Zhang,Jiulong Zhang,Xinlong Zang,Mingxi Qiao,Xiuli Zhao,Haiyang Hu,Dawei Chen
Multidrug resistance (MDR) has been a major obstacle to tumor chemotherapy. Pluronic unimers have been reported to be promising copolymers to reverse MDR, and the intracellular delivery of Pluronic unimers is a problem worth thinking. To exert the excellent reversal effect of Pluronic unimers, DOX-loaded G4.0 PAMAM was modified with PluronicL64 via cis-aconitic acid as a pH-sensitive linkage (PCPAMAM/DOX), which could release DOX and Pluronic unimers into cytoplasm. The Pluronic-modified PAMAM (PCPAMAM) exhibited favorable biocompatibility and pH-sensitivity. PCPAMAM/DOX showed a nano-scale size and a sustained in vitro release profile. Compared with a control formulation, PCPAMAM/DOX showed a higher reversal effect on MCF-7/ADR cells and enhanced intracellular drug accumulation. The results of P-gp activity, subcellular distribution of PluronicL64, the ATP level and mitochondrial transmembrane potential all illustrated that free Pluronic unimers could be released by PCPAMAM functioning as reversal agents. In conclusion, PCPAMAM could be a promising vehicle to enhance DOX accumulation by overcoming MDR in MCF-7/ADR cells. This work also provided an effective method to deliver Pluronic unimers into MDR cells.
研究领域:
药剂学
·
癌症研究
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）