Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Zixuan Li,Ziyi Yang,Qinghong Shi,Yan Sun
Environmentally widespread endocrine-disrupting chemicals, such as bisphenol A (BPA), have posed a risk to living things. Laccase, a kind of oxidoreductase, can effectively catalyze the degradation of phenolic compounds like BPA. Herein, a defective metal-organic framework (dPCN-224) with BPA adsorption capability was synthesized and used for laccase immobilization. The material was extensively characterized and successful immobilization of laccase via hydrophobic interaction was confirmed. The immobilization reached an enzyme loading density of 80 mg/g onto dPCN-224 and the laccase@dPCN-224 showed high activity recovery (90.0% vs. free laccase) and increased thermostability, pH tolerance, and organic co-solvent resistance over the free enzyme. Moreover, at various concentrations (0.5–20 mg/L), the BPA removal efficiencies of laccase@dPCN-224 were 3.3–5.0 times higher than those of free laccase due to the cooperative effect of adsorption and enzymatic degradation. The BPA bound on laccase@dPCN-224 could be almost completely biodegraded by adding 0.5 mM of electro-mediator (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). In addition, after five consecutive batch cycles, the BPA removal efficiency of laccase@dPCN-224 kept as high as 98.1% at 5 mg/L BPA. Taken together, the results proved the potential of laccase@dPCN-224 for applications in addressing environmental BPA pollutions.
研究领域:
环境科学
·
生物技术
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DOI
高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
JunHui Wei,Tingting Wang,Jin Tao,Xuezhi Wang,Kangning Li,Junnan Kan,Yijie Lv,Kai Qi,Ailing Wang,Xianglin Li,Caixia Yang
Breast cancer remains a major clinical challenge due to its high incidence, tumor heterogeneity, hypoxia, and limited treatment specificity. In this study, we developed PMDH, a six-in-one integrated nanoplatform for targeted multimodal imaging and combined therapy. PMDH was constructed by sequentially coating a MnO 2 shell on PCN–224, loading doxorubicin (DOX), and conjugating hyaluronic acid (HA). The system features complementary tumor microenvironment (TME) responsive imaging modalities, including fluorescence imaging, magnetic resonance imaging, and photoacoustic imaging for precise diagnostics. Therapeutically, PMDH enables controlled DOX release for chemotherapy, self-enhanced photodynamic therapy (PDT) through intratumoral oxygen generation, and PDT-triggered immunotherapy. These integrated capabilities were comprehensively validated through in vitro and in vivo studies, underscoring PMDH’s potential as a versatile and precise platform for oncology treatment.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
·
IF 3.131
Tong Li,Pei Hu,Jiawei Li,Pintong Huang,Weijun Tong,Changyou Gao
Inspired by natural hemin structure, the fabrication of metal organic framework (MOF) nanomaterials constructed by metal ions and iron porphyrin for peroxidase mimicking has attracted extensive attention recently due to their high concentration of active sites and great channeling for substrate diffusion. Herein, by using benzoic acid as capping agent, we fabricated uniform PCN-224 nanoparticles (NPs), which were composed of Zr 6 cluster and tetrakis(4-carboxyphenyl)porphyrin (H 2 TCPP). Then Fe(Ⅲ) ions were incorporated into the center of H 2 TCPP producing iron porphyrin unit similar to hemin serving as catalytic active site. The procedure of iron incorporation didn't change the size and morphology of PCN-224 NPs. The newly formed Fe@PCN-224 NPs possessed peroxidase-like activity with much lower K m values and higher K cat values than most of peroxidase-like nanozymes as well as natural horseradish peroxidase (HRP), indicating their enhanced catalytic activity. Taking advantage of the excellent peroxidase-like activity of Fe@PCN-224 NPs, we successfully developed a colorimetric method for simple and sensitive detection of hydrogen peroxide and glucose in combination with glucose oxidase .
研究领域:
生物化学
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纳米医学
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DOI
高影响因子
International Journal of Pharmaceutics-X
·
2026
·
IF 7.9
Xiang Wang,Hengrui Li,Le Wang,Yihan Ma,Miao Qin,Ruonan Sun,Jing Hu
Tumor hypoxia is a major barrier to oxygen-dependent photodynamic therapy (PDT), markedly limiting its therapeutic efficacy in hepatocellular carcinoma (HCC). Herein, a self‑oxygenating and redox-amplifying metal-organic framework (MOF) nanoplatform is constructed by co-loading sorafenib (Sor) and plumbagin (PLB) into a surface galactose functionalized Mn-porphyrinic PCN-224(Mn) (PM) framework to afford SorPLB@Gal-PM for asialoglycoprotein receptor (ASGPR)-mediated HCC targeting. The Mn-porphyrin centers endow SorPLB@Gal-PM with catalase-like activity, enabling in situ decomposition of endogenous H 2 O 2 to generate O 2 and thereby sustaining 1 O 2 -based PDT under irradiation. Meanwhile, Sor/PLB co-delivery, together with PLB-mediated GPX4 suppression, promotes oxidative stress amplification. In hypoxic liver cancer stem cell-like C5WN1 cells, SorPLB@Gal-PM elicits markedly enhanced intracellular ROS upon irradiation, showing approximately 3.4-fold and 2.9-fold higher DCFH-DA signals than SorPLB@Gal-PCN-224 and PM, respectively, accompanied by downregulation of hypoxia-inducible factor-1α (HIF-1α) and glutathione peroxidase 4 (GPX4). In a C5WN1 tumor-bearing nude mouse model, SorPLB@Gal-PM combined with irradiation achieves the most potent tumor suppression with a tumor inhibition rate of 83.70% ± 4.12%. Collectively, SorPLB@Gal-PM provides a hypoxia-adaptive nanoplatform integrating Mn-assisted self‑oxygenation and redox-amplified chemo-PDT for effective HCC therapy.
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Jing Zhao,Chaoyi Lyu,Rui Zhang,Yao Han,Yundi Wu,Xilong Wu
The expansion of large-scale nuclear power causes a substantial volume of radioactive wastewater containing uranium to be released into the environment. Because of uranium's toxicity and bioaccumulation, it is critical to develop the efficient and sustainable materials for selective removal of uranium (VI). Herein, a regenerable anti-biofouling nano zero-valent iron doped porphyrinic zirconium metal–organic framework (NZVI@PCN-224) heterojunction system was successfully fabricated. Due to the Schottky-junction effect at the NZVI/MOF interface, the NZVI nanomaterial immobilized on PCN-224 could improve interfacial electron transfer and separation efficiency, and enhance entire reduction of highly soluble U(VI) to less soluble U(IV), involving photocatalytic reduction and chemical reduction. Meanwhile, the photocatalytic effect also prompts the NZVI@PCN-224 to produce more biotoxic reactive oxygen species (ROS), resulting in high anti-microbial and anti-algae activities. Under dark conditions, NZVI@PCN-224 with a large specific surface area could provide sufficient oxo atoms as the uranium binding sites and show the highest uranium-adsorbing capability of 57.94 mg/g at pH 4.0. After eight adsorption-desorption cycles, NZVI@PCN-224 still retained a high uranium adsorption capacity of 47.98 mg/g and elimination efficiency (91.72%). This sorption/reduction/anti-biofouling synergistic strategy of combining chelation, chemical reduction and photocatalytic performance inspires new insights for highly efficient treatment of liquid radioactive waste .
研究领域:
环境科学
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材料科学
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核废料处理
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
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IF 8.5
Xu Xu,Tongxin Sun,Xinyue Zhou,Zhuang Liu,Lei Zhang
The nanozyme-based colorimetric sensor shows promise for rapid pesticide detection but struggles with non-specific enzyme inhibition. This study developed a portable paper-based sensor for detecting the propiconazole (PC) pesticide using Fe@PCN-224 nanocubes (NCs). Characterization confirmed the successful synthesis of Fe@PCN-224 NCs, which displayed peroxidase-like activity. The specific interaction between PC's triazole ring and the Fe active site inhibited their activity, enabling selective detection with a limit of 8 × 10 −9 mol L −1 and a linear range of 0.03 × 10 −6 to 0.90 × 10 −6 mol L −1 . Kinetic studies revealed a Michaelis-Menten constants ( K m ) of 0.68 × 10 −3 mol L −1 for TMB, indicating higher affinity in Fe@PCN-224 NCs. The electron paramagnetic resonance (EPR) analysis revealed the production of •OH, 1 O 2 , O 2 • − during the catalytic reactions. By integrating smartphone technology, this portable sensor achieved recoveries from vegetable samples between 94.6 % and 109.2 %, demonstrating its potential as an accurate, cost-effective analytical tool for food safety and advancing nanozyme applications.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Wenxu Song,Xinrui Cao,Wendi Yue,Fengchun Zhao,Qing Zhou,Zhengyou Yang
As a carcinogenic mycotoxin, Aflatoxin B1 (AFB1) is widespread in grains. In particular, corn-derived feed and food are more heavily contaminated with AFB1 than other grain products, which pose severe risks to food safety and human health. Thus, developing rapid, sensitive, and accurate AFB1 detection methods is of great importance. In this study, a highly sensitive photoelectrochemical (PEC) immunosensor for detecting AFB1 based on phage-anti-immunocomplex peptide and the PCN-224@MXene composite was developed. The prepared phage-anti-immunocomplex peptide could bind to a large number of enzyme-labeled secondary antibodies, thereby greatly increasing the loading capacity of horseradish peroxidase (HRP). Moreover, the PCN-224@MXene showed wider light absorption range and narrower band gap, which is conducive to improving the photoelectric conversion efficiency. This resulted in the photocurrent intensities of PCN-224@MXene being 1.9-fold and 2.5-fold higher than those of PCN-224 and MXene, respectively. In addition, HRP catalyzed the oxidation of 4-Chloro-1-naphthol by H 2 O 2 to product an insulating precipitate, which hindered photo-induced electron transport, and thereby lead to a distinct photocurrent change. Using the multi-signal amplification strategy, the sensitivity of the PEC immunosensor for detecting AFB1 was significantly enhanced. Under the optimal detection conditions, the linear range was from 1 fg mL -1 to 100 ng mL -1 , and the detection limit was as low as 0.91 fg mL -1 (3σ/S). This LOD was considerably lower than the regulatory limit for AFB1 in corn. This method showed good selectivity, stability and reproducibility, and could be applied to the detection of AFB1 in corn samples.
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
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IF 10.4
Jiayu Chen,Chuang Yang,Runzhong Yu,Han Zhang,Liyuan Zhang
Alternariol (AOH) is an emerging mycotoxin widely found in agricultural products. It has drawn attention due to the potential health risks associated with long-term dietary exposure. Therefore, a ratiometric fluorescent aptamer sensor based on PCN-224 was developed. By combining the intrinsic fluorescence properties of PCN-224 with aptamer recognition, sensitive detection of AOH was achieved. PCN-224 served as both a fluorescence platform and a source of internal reference signals, enabling self-calibrated ratio analysis. Under optimal conditions, the sensor exhibited good linear response in the range of 0.001–500 ng/mL. The LOD was 0.449 pg/mL. It also exhibited good selectivity, stability, and reproducibility. This sensor has been successfully applied to the detection of AOH in cereal samples, demonstrating its reliability in the analysis of complex food matrices. This work provided an effective strategy for monitoring mycotoxins by combining MOF fluorescence regulation with aptamer recognition.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Jinjuan Xue,Jiaqian Zhang,Meng Yuan,Yiting Lv,Zhaoxia Chen,Mingxin Wang
The development of novel photocatalytic platform for efficient water purification and sterilization is of utmost importance due to the significant threat of chronic diseases resulting from water pollution and bacterial contamination. Herein, a high-efficiency, easy-recyclable, and environment-friendly photocatalytic fabric was developed as a water disinfection and organic pollutant degradation platform. In which, microamounts of Au nanoparticles (2 wt%) and Cu(II) clusters (2.5 wt%) co-decorated porphyrinic MOF (Au/PCN-224/Cu(II)) coating was applied as photocatalytic surface, and flexible cotton fabric served as stable support. Au/PCN-224/Cu(II) composite photocatalyst was prepared via a facile photo-deposition procedure followed by impregnation method, and further immobilized onto polydopamine (PDA) pre-treated cotton fabric through catechol-amine reaction. The fabricated photocatalytic platform exhibited excellent ability to generate reactive oxygen species (ROS) by multiple routes under low-power visible light irradiation, and achieved efficient photodisinfection effects for causative organism including Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus aureus ( S. aureus ) without the obvious leakage of metal ions from the potocatalyst during the reaction. Additionally, the reaction rate constant for organic dye MB photodegradation achieved by Au/PCN-224/Cu(II)@Fabric was 3.73 times higher than that of PCN-224@Fabric, and the degradation intermediates analysis was also conducted. The band alignments of PCN-224, Au/PCN-224, Au/PCN-224/Cu(II) photocatalysts were determined, and the charge transfer mechanism within Au/PCN-224/Cu(II) photocatalyst was elucidated through the utilization of DRS, Mott − Schottky, ESR, EIS, and photocurrent analyses. It is suggested that the deposition of Au and doping of Cu(II) both with small amounts enhanced the light absorption capacity, and also improved the charge separation ability through the surface plasmon resonance (SPR) effect and interfacial electron transfer (IFCT) mechanism. Moreover, Au SPR and Cu doping exhibited a substantial enhancement in photothermal conversion effect, thereby synergistically reinforcing the sterilization performance. This work provides an approach for the development of effective photocatalytic platform based on porphyrinic MOFs combined with trace metal co-catalysts in the application of water disinfection and purification.
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高影响因子
Small
·
2023
·Vol. 19
·期 16
·2206124
·
IF 13.3
Futing Yang,Wenjie Yu,Qiying Yu,Xiyu Liu,Chunping Liu,Chong Lu,Xinghua Liao,Yi Liu,Na Peng
The apoptosis-resistant mechanism of photodynamic therapy (PDT) usually results in limited therapeutic efficacy. The development of new strategies for sensitizing targeted ferroptosis that bypass apoptosis resistance is of great significance to improve the antitumor efficacy of PDT. In this study, a novel amphiphilic copolymer whose main chain contains reactive oxygen species (ROS)-responsive groups and the end of side chains contains triphenylphosphine is synthesized, to encapsulate porphyrinic metal–organic framework PCN-224 via self-assembly which are hydrothermally synthesized by coordination of zirconium (IV) with tetra-kis(4-caboxyphenyl) porphyrin, and loaded carbon monoxide releasing molecule 401 (CORM-401) by their hollow structures (PCN-CORM), and finally, surface-coated with hyaluronic acid. The nanosystem can sequentially localize to mitochondria which is an important target to induce apoptosis and ferroptosis in cancer cells. Upon excitation with near-infrared light, PCN-224 is activated to produce amounts of ROS, and simultaneously triggers the rapid intracellular release of CO. More importantly, the released CO can sensitize ferroptosis and promote apoptosis to significantly enhance the antitumor efficacy of PCN-224 both in vitro and in vivo. These results illustrate that the mitochondria-targeted drug delivery system combined PDT with CO leads to an effective antitumor efficacy, which maybe a promising way to enhance the treatment efficiency of PDT.
研究领域:
药学
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生物医学工程
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肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
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IF 9.4
Zheng Wang,Hui Li,Chuang Jiang,Wenya Liu,Siyu Zhang,Yibin Zhou,Kang Liu,Yaqing Xiao,Ruyan Hou,Xiaochun Wan,Yingnan Liu
Herein, a novel dual-mode probe for organophosphorus pesticides (OPs) colorimetric and photothermal detection was developed based on manganese modified porphyrin metal–organic framework (PCN-224-Mn). PCN-224-Mn had excellent oxidase-like activity and oxidized colorless 3,3,5,5-tetramethylbenzidine (TMB) to blue–green oxidation state TMB (oxTMB), which exhibited high temperature under near-infrared irradiation. l -ascorbate-2-phosphate was hydrolyzed by acid phosphatase to produce ascorbic acid, which weakened colorimetric and photothermal signals by impacting oxTMB generation. The presence of OPs blocked the production of ascorbic acid by irreversibly inhibiting the activity of acid phosphatase, causing the restoration of chromogenic reaction and the increase of temperature. Under the optimal conditions, the probe showed a good linear response to OPs in the concentration range of 5 ∼ 10000 ng/mL, using glyphosate as the analog. The detection limits of glyphosate in colorimetric mode and photothermal mode were 1.47 ng/mL and 2.00 ng/mL, respectively. The probe was successfully used for sensitive identification of OPs residues in tea, brown rice, and wheat flour. This work proposes a simple and reliable colorimetric/photothermal platform for OPs identification, which overcomes the problem that single-mode detection probes are susceptible to external factors, and has broad application potential in the field of food safety.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 15
·2202280
·
IF 10
Wenya Li,Rongtian Li,Qiang Ye,Yiming Zou,Xing Lu,Wenhua Zhang,Jinxiang Chen,Yinghua Zhao
Nanomedicine exhibits emerging potentials to deliver advanced therapeutic strategies in the fight against triple-negative breast cancer (TNBC). Nevertheless, it is still difficult to develop a precise codelivery system that integrates highly effective photosensitizers, low toxicity, and hydrophobicity. In this study, PCN-224 is selected as the carrier to enable effective cancer therapy through light-activated reactive oxygen species (ROS) formation, and the PCN-224@Mn 3 O 4 @HA is created in a simple one-step process by coating Mn 3 O 4 nanoshells on the PCN-224 template, which can then be used as an “ROS activator” to exert catalase- and glutathione peroxidase-like activities to alleviate tumor hypoxia while reducing tumor reducibility, leading to improved photodynamic therapeutic (PDT) effect of PCN-224. Meanwhile, Mn 2+ produced cytotoxic hydroxyl radicals (∙OH) via the Fenton-like reaction, thus producing a promising spontaneous chemodynamic therapeutic (CDT) effect. Importantly, by remodeling the tumor microenvironment (TME), Mn 3 O 4 nanoshells downregulated hypoxia-inducible factor 1 α expression, inhibiting tumor growth and preventing tumor revival. Thus, the developed nanoshells, via light-controlled ROS formation and multimodality imaging abilities, can effectively inhibit tumor proliferation through synergistic PDT/CDT, and prevent tumor resurgence by remodeling TME.
研究领域:
纳米技术
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癌症治疗
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）