JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Ning Ding,Yaxi Wang,Xiaolin Wang,Wei Chu,Tian Yin,Jingxin Gou,Haibing He,Yu Zhang,Yanjiao Wang,Xing Tang
Gemcitabine , as a nucleoside analog , has been used as first-line chemotherapy drug for many years. However, the treatment dose of gemcitabine in the clinic has been usually extremely high due to its rapid metabolism. The aim of this study was to achieve successful chemotherapy at low doses by a PEGylated liposomal delivery system. The liposomes were prepared with Lipoid S-100, Cholesterol and DSPE-PEG2000 (at a molar ratio of 9:2:0.07) by the film dispersion-extrusion-ammonium sulfate gradient method. The entrapment efficiency (EE, %) and drug loading (DL, %) of the liposomes (117.8 ± 16.85 nm) were 68.83% and 2.52%, respectively. The in vitro release study and plasma stability experiments showed the gemcitabine liposomes (GEM-Lip) was more stable than gemcitabine solutions (GEM-Sol) at pH 7.4 and in the plasma. AUC and C max of GEM-Lip were shown to be 6.20 and 16.44-fold higher than that of the solutions. The pharmacodynamics (PD) experiment in Kunming mice bearing a H22 cancer cell model showed that the tumor inhibition rate (TIR) of GEM-Lip was 6.25-fold as that of GEM-Sol when administrated intravenously. Our findings demonstrated that the preparation of GEM-Lip could effectively enhance the pharmacokinetic properties , the plasma stability and antitumor effect of gemcitabine.
研究领域:
药学
·
肿瘤学
·
生物医学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Jiahao Lin,Qiang Cai,Yinian Tang,Yanjun Xu,Qian Wang,Tingting Li,Huihao Xu,Shuaiyu Wang,Kai Fan,Zhongjie Liu,Yipeng Jin,Degui Lin
Highly ordered mesoporous silica nanoparticles (MSNs) with pore diameter of 2.754 nm and particle size of 115 ± 15 nm were prepared with etching method. Homogeneous PEGylated lipid bilayer with 10–15 nm thickness was coated around the surface of MSNs using film hydration method. Systematic optimization and characterization of co-encapsulation process of paclitaxel (Tax) and curcumin (Cur) into PEGylated lipid bilayer coated mesoporous silica nanoparticles (PLMSNs) were performed carrying out single factor test, associated with Box-Behnken Design. The concentration of encapsulated drugs was measured by reversed phase high performance liquid chromatography (RP-HPLC) method. Optimal factor settings were as follows: 50 mg MSNs, ratio of MSNs to lipid (w/w) = 1:1.11, and ratio of lipid to CHO (w/w) = 3.93:1. The average experimental EE Tax , EE Cur and stability score value were (77.48 ± 2.73) %, (30.70 ± 3.56) % and 4 point respectively based on the conditions mentioned above. Morphology determination of Tax-Cur-PLMSNs revealed that the composite nanoparticles were spherical particals with uniform dispersion. In vitro release experiment indicated that PLMSNs improved dissolution of Tax compared to Tax powder suspension and exhibited sustained release property. Tax-Cur-PLMSNs manifested definite and persistently promoted cytotoxic effect against canine breast cancer cells. This prolonged and enhanced activity of Tax-Cur-PLMSNs might contribute to its sustained release effect.
研究领域:
药剂学
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Haitao Huang,Tao Yue,Ke Xu,Jafar Golzarian,Jiahui Yu,Jin Huang
Gd(III) chelate is currently used as positive magnetic resonance imaging (MRI) contrast agent in clinical diagnosis, but generally induces the risk of nephrogenic systemic fibrosis (NSF) due to the dissociated Gd 3+ from Gd(III) chelates. To develop a novel positive MRI contrast agent with low toxicity and high sensitivity, ultrasmall MnO nanoparticles were PEGylated via catechol-Mn chelation and conjugated with cRGD as active targeting function to tumor. Particularly, the MnO nanoparticles with a size of ca. 5 nm were modified by α,β-poly(aspartic acid)-based graft polymer containing PEG and DOPA moieties and, meanwhile, conjugated with cRGD to produce the contrast agent with a size of ca. 100 nm and a longitudinal relaxivity ( r 1 ) of 10.2 mM −1 S −1 . Such nanoscaled contrast agent integrated passive- and active-targeting function to tumor, and its efficient accumulation behavior in tumor was verified by in vivo distribution study. At the same time, the PEG moiety played a role of hydrophilic coating to improve the biocompatibility and stability under storing and physiological conditions, and especially might guarantee enough circulation time in blood. Moreover, in vivo MRI revealed a good and long-term effect of enhancing MRI signal for as-fabricated contrast agent while cell viability assay proved its acceptable cytotoxicity for MRI application. On the whole, the as-fabricated PEGylated and cRGD-functionalized contrast agent based on ultrasmall MnO nanoparticles showed a great potential to the T 1 -weighted MRI diagnosis of tumor.
研究领域:
生物医学工程
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影像医学
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材料科学
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DOI
PubMed
高影响因子
POLYMER
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2020
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IF 4.231
Dianliang Zhang,Desheng Qi,Jiayu Wang,Shuangjiang Yu,Chaoliang He,Mingxiao Deng
Injectable hydrogels have attracted great research interest for the delivery drugs and recruitment of cells to promote tissue regeneration. In this work, a series of the amino acid based methoxy-poly (ethylene glycol)- b -(poly ( γ -ethyl- l -glutamate- co / b - l -phenylalanine) block copolymers (mPEG- b -P (ELG- co / b -Phe)s) with similar block chain length, but different proportion and sequence of γ -ethyl- l -glutamate (ELG) and l -phenylalanine (Phe) arrangements were prepared through ring-opening polymerization method. The influence of the ratio and sequence structures of mPEG- b -P (ELG- co / b -Phe)s on their thermosensitive aqueous gelation behaviors were investigated in detail. The results showed that the obtained polymers showned higher modulus (G’) and lower critical gelation temperature with higher proportion of l -phenylalanine. However, the physical properties start to deteriorate as the copolymers have a large number of phenylalanine groups. To clarify this phenomenon, the analysis of secondary structure with circular dichroism (CD) and fourier transform infrared (FTIR) spectrum showed that random coil reached a higher percentage as incorporated phenylalanine groups increased. On the other hand, the polymers with different sequence and similar compositions exhibited divergence due to the influence of random coil conformation. In addition, the good biocompatibility of mPEG- b -P (ELG- co -Phe)s were observed in vitro and in vivo . The copolymers have the potential to be used as thermo-sensitive injectable hydrogels for biomedical applications.
研究领域:
生物材料学
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高分子化学
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药物递送系统
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
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IF 5.158
Ke Wang,Jialang Zhuang,Yubing Liu,Maosheng Xu,Jingyuan Zhuang,Zuanguang Chen,Yen Wei,Yuanqing Zhang
It is of great significance to construct multifunctional nanosystems for simultaneous imaging and therapy of cancer cells. Herein, PEGylated chitosan nanoparticles with embedded bismuth sulfide were facilely fabricated via reverse-microemulsion method for fluorescent imaging and photothermal therapy of HepG2 cells. The obtained BSA-Bi 2 S 3 -CG-PEG nanospheres revealed dual-wavelength fluorescence, which were spectrally isolated from the bioautofluorescence. Moreover, they demonstrated remarkable photothermal conversion efficiency and stability. Importantly, these small BSA-Bi 2 S 3 -CG-PEG nanoparticles shown a zeta potential of + 42.3 mV, which could rapidly get into HepG2 cells and locate in the cytoplasm and nuclei of cells. Based on their excellent photothermal effect and high cellular uptake, BSA-Bi 2 S 3 -CG-PEG nanoparticles could efficiently kill HepG2 cells under an 808 nm laser irradiation. This construction strategy can be used for preparation of fluorescent chitosan nanoparticles with other therapeutic agents embedded, which would provide a versatile platform for dual-wavelength fluorescent imaging guided therapy of cancer.
研究领域:
生物医学工程
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癌症治疗研究
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材料科学
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产品关联
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2023
·Vol. 301
·期 12
·1475-1486
·
IF 2.4
Li Yinglu,Zhao Jing,Wang Yurui,Zhao Tianfu,Song Yanan,Liang Fei
Nanoscale metal organic frameworks (NMOFs) have become excellent drug carriers for the treatment of cancer or other diseases, due to the advantages of large surface area, high drug loading, tunable structure and easy functionalization, with potential application in the field of drug delivery. In this paper, UiO-66 NMOF was synthesized using terephthalic acid as organic ligand and zirconium chloride as metal salt, and then biocompatible chitosan (CS) and PEGylated chitosan (CS-g-mPEG)-decorated NMOFs as drug carriers were constructed by surface modification, using 5-FU as the drug model. The structures, size and morphology were characterized by IR, XRD, DLS, SEM and TEM respectively. The results showed that when the concentration was 0.0872 M, the molar ratio was 1:1 and the acetic acid amount was 2.9 mL, the average particle size of NMOFs was 145.60 nm and 171.73 nm, respectively. The drug loading capacity and encapsulation rate could reach 15.7% and 52.8%. The in vitro controlled drug release results indicated that the release rate accelerated with increasing temperature and decreasing initial amount, greatly reduced under acidic conditions, and the coating of CS and CS-g-mPEG on the surface of UiO-66 had the effect of controlling drug release, further improved the bioavailability of 5-FU. The decorated UiO-66 NMOFs show excellent biocompatibility as nanodrug carriers and can avoid rapid degradation under gastric acid, which are more suitable for oral drug delivery.
研究领域:
材料科学
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药学
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生物医学工程
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产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 33
·11458-11464
·
IF 4
Xuejiao Li,Bo Li,Wenbo Zhang,Zimo Chen,Jinping Liu,Yu Shi,Huanyan Xu,Lianwei Shan,Xin Liu,Limin Dong
Phototherapy, including photothermal and photodynamic therapy, has gained extensive attention in the tumor treatment field recently, while synergistic therapy can significantly improve curative effects. However, a complicated photo-responsive nanosystem, different excitation wavelengths, and low tissue depth hindered its actual application. Herein, single near-infrared responsive PEGylated defective MoO2 nanocrystals were fabricated by a green hydrothermal method. The photothermal and photodynamic performances of the samples were presented in detail under a safe power of 1064 nm (NIR-II, 1.0 W cm−2). Interestingly, the photodynamic properties were prompted by the localized surface plasmon resonance (LSPR) photothermal effect obviously, and the collaborative enhancement mechanism was explored in depth. Subsequently, the in vitro cytotoxicity was evaluated on the 4T1 cancer cells under NIR-II irradiation. This work may provide guidance for the facile fabrication of TMOs for NIR-II responsive and enhanced dual-modal phototherapy.
研究领域:
肿瘤治疗
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纳米医学
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生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2021
·
IF 6.598
Xianyan Qin,Liming He,Donghao Fan,Wenlang Liang,Qin Wang,Jiyu Fang
Rheumatoid arthritis (RA) is a common autoimmune disease characterized by joint inflammation and immune dysfunction. Although various therapeutic approaches have been utilized for the treatment of RA in clinical applications, the low responsiveness of RA patients and undesired systemic toxicity are still unresolved problems. Targeting the resolution pathway of inflammation with pro-resolving mediators would evoke the protective actions of patient for combating the inflammation. Ac2–26, a 25-amino acid peptide derived from Annexin A (a pro-resolving mediator), has shown good efficacy in the treatment of inflammatory disorders. However, the low bioavailability of Ac2–26 peptides hinders their efficacy in vivo . In this paper, we formed PEGylated lipid nanoparticles (LDNPs) by the co-assembly of l -ascorbyl palmitate (L-AP) and N-(carbonyl methoxypolyethylene glycol-2000)-1,2-distearoyl-sn‑glycero-3-phosphoethanolamine (DSPE-PEG 2k ) to encapsulate and deliver Ac2–26 peptides to the arthritic rats. They showed good stability and biocompatibility. After being intravenously administrated, Ac2–26 peptide-loaded PEGylated lipid nanoparticles (ADNPs) showed the prolonged in vivo circulation time and enhanced accumulation in inflamed sites. In vivo therapeutic evaluations revealed that ADNPs could attenuate synovial inflammation and improve joint pathology. Therefore, the pro-resolving therapeutic strategy using ADNPs is effective in RA treatment.
研究领域:
免疫学
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纳米医学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 40
·6241-6249
·
IF 5.687
Junjie Yan,Rongrong Wang,Donghui Pan,Runlin Yang,Yuping Xu,Lizhen Wang,Min Yang
Fluorescent poly(amide-imide)s are promising materials for use in photonics due to their controllable optical properties and excellent flexibility. Although unconventional fluorescent polymers have been flourishing recently, the fluorescence of aliphatic poly(amide-imide)s has never been realized until now. Their poor solubility and intolerance to reactive groups during synthesis prohibit access for the modification of poly(amide-imide)s. Here, we synthesize a functional thiolactone-maleimide monomer via copper(I)-catalyzed azide alkyne cycloaddition and employ a strategy combining aminolysis of thiolactones and amine-maleimide Michael addition for the synthesis of fluorescent aliphatic poly(amide-imide)s with 2-aminosuccinimide fluorophores. Moreover, in situ generated thiols enable the poly(amide-imide)s to undergo facile PEGylation via a thiol–methacrylate Michael addition reaction to accomplish excellent solubility in organic solvents and in water.
研究领域:
材料科学
·
化学合成
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Anlin Yin,Weihua Zhuang,Gongyan Liu,Xiaorong Lan,Zhonglan Tang,Yan Deng,Yunbing Wang
The fabrication of a functional small-diameter vascular graft with good biocompatibility, in particular hemocompatibility, has become an urgent clinical necessity. We fabricated a native bilayer, small-diameter vascular graft using PEGylated chitosan (PEG-CS) and poly (L-lactic acid-co-ε-caprolactone; PLCL). To stabilize the inner layer, a PEG-CS blend with PLCL at ratio of 1:6 was casted on a round metal bar by a drip feed, and the outer layer, a PLCL blend with water-soluble PEG that acted as a sacrificial part to enhance pore size, was fabricated by electrospinning. The results showed excellent hemocompatibility and strong mechanical properties. In vitro , the degradation of the graft was evaluated by measuring the graft structure, mass loss rate, and changes in molecular weight. The results indicated that the graft had adequate support for the regeneration of blood vessels before collapse. An in vivo study was performed in a canine femoral artery model for up to 24 weeks, which demonstrated that the PEGylated bilayer grafts possessed excellent structural integrity, high compatibility with blood, good endothelial cell (EC) and smooth muscle cell (SMC) growth, and high expression levels of angiogenesis-related proteins, features that are highly similar to autologous blood vessels. Moreover, the results showed almost negligible calcification within 24 weeks. These findings confirm that the bilayer graft mimics native cells, thereby effectively improving vascular remodeling.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Liming He,Donghao Fan,Wenlang Liang,Qin Wang,Jiyu Fang
Rheumatoid arthritis (RA) is an autoimmune disorder. It causes inflammation, swelling, and pain in the joints of the human body. Overexpressed matrix metalloproteinases (MMPs) at the inflammatory sites of RA are a target in the construction of inflammation-responsive drug delivery vehicles for enhancing the therapeutic effect of anti-inflammatory drugs in the treatment of RA. In this paper, we report MMP-responsive PEGylated lipid nanoparticles through the co-assembly of triglycerol monostearate (TGMS) and 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-poly(ethyleneglycol) (DSPE-PEG2000) in which the ester bond of TGMS is cleavable by MMPs and the PEG chain provides a stealth layer. The lipid nanoparticles show high biocompatibility, extended blood circulation, and preferential distribution in the inflammatory joints of RA. The loaded dexamethasone (Dex) can be rapidly released from the lipid nanoparticles in response to MMPs. After being intravenously administered to arthritic rats, Dex-loaded MMP-responsive PEGylated lipid nanoparticles significantly reduce the degree of joint swelling and inhibit the production of TNF-α and IL-1β in joint tissues. These results demonstrate that MMP-responsive PEGylated lipid nanoparticles are a smart drug vehicle for the treatment of RA with improved therapeutic efficacy.
研究领域:
药剂学
·
免疫学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
CARBON
·
2018
·
IF 7.082
Yiwen Hu,Yongxin Wang,Zhixiang Zeng,Haichao Zhao,Xuewu Ge,Kai Wang,Liping Wang,Qunji Xue
Dispersion of Graphene in water is conducive to their practical applications as lubricating additive in water-based fluids due to their safety, low cost and environmental-friendliness. But owing to its hydrophobicity , it is difficult to achieve stable aqueous graphene suspension. Here, we use the Toluene-2,4-diisocyanate as bridging agent between hydrophilic Poly (ethylene glycol) (PEG) and ethanolamine to prepare the amino-containing PEG (PEG-NH 2 ), followed by covalently grafting PEG-NH 2 onto the surface of carboxylaed graphene (G-COOH) nanosheets to synthesize the PEGlated graphene (PEG-G) via facile amidation under mild conditions. The physical, chemical properties of product were characterized by Fourier transformed infrared spectra (FTIR), Raman spectra , X-ray photo electron spectroscopy (XPS) and so on. Transmission electron microscopy (TEM) and scanning probe microscope (SPM) were utilized to observed the morphology of G-COOH and PEG-G. The frictional properties of PEG-G as nanoadditive of water-based lubricant were characterized by UMT-3 tribometer, following with analyzes on wear surfaces by 3D laser scanning confocal microscopy . Based on the results of characterization, the PEG-G was prepared successfully and the dispersion shows superior friction-reducing and anti-wear properties owing to the formation of fluid adhesive film and carbon protective film, especially the concentration of PEG-G in water is 0.05 wt%.
研究领域:
材料科学
·
纳米科技
·
表面工程
·
摩擦学
·
高分子化学
·
应用化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）