MOLECULES
·
2024
·Vol. 29
·期 5
·1081
·
IF 4.2
Jieqiong Ding,Jie Su,Binhua Luo,Liqiong Ding
Lycorine is a kind of natural active ingredient with a strong antitumor effect. In this study, folate ligand-conjugated polyethylene glycol-block-poly(l-lactide) (PEG-PLLA) nanoparticles (FA-PEG-PLLA NPs) were designed to deliver lycorine to enhance its anti-glioma activity. The successful preparation of the FA-PEG-PLLA polymer was confirmed by1H-NMR, FT-IR, XRD, TGA, and DSC. The optimal formulation for LYC@FA-PEG-PLLA NPs was determined by response surface analysis as follows: sodium dodecyl sulfate (SDS) of 1%, carrier material of 0.03 g, organic phase volume of 3 mL, and ultrasonic power of 20%. The LYC@FA-PEG-PLLA NPs exhibited an encapsulation efficiency of 83.58% and a particle size of 49.71 nm, demonstrating good stability. Hemolysis experiments, MTT assays, and cell scratch assays revealed excellent biocompatibility of FA-PEG-PLLA and superior anti-glioma activity of LYC@FA-PEG-PLLA NPs compared to the raw drug. Additionally, cell apoptosis assays, ROS experiments, and western blot analysis demonstrated that LYC@FA-PEG-PLLA NPs contributed to cell apoptosis by inducing ROS generation and increasing the expression of NF-κB inhibitory protein IκBα. These results suggested that LYC@FA-PEG-PLLA NPs hold promise for glioma treatment.
研究领域:
药学
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纳米技术
·
肿瘤学
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Xin Sun,Bai Xue,Shengdu Yang,Kangwei Huo,Xingyan Liao,Xujuan Li,Lan Xie,Shuhao Qin,Qiang Zheng
Focus on the potential application in oil-water separation for porous and biodegradable Poly(L-latic acid) (PLLA) amenable to economically viable water treatment, we designate the controllable pores, fibrillary network and protrusion structure by crystallization induced phase separation in poly(L-latic acid)/Zinc oxid (PLLA/ZnO) materials, ready for their potential application in oil-water separation. It’s worth noting that the interface effect between PLLA and ZnO is strengthened by silane coupling agent KH570. The crystallinity of the PLLA/ZnO@KH570 materials are correspondingly increased from 36.9% to 41.1% as the slightly increasing content of ZnO@KH570 from 1% to 7%, indicating that ZnO@KH570 nanoparticles can act as heterogeneous nucleating agent to promote crystallization of PLLA, which facilitate the conversion of PLLA solution from liquid-liquid phase separation mechanism to solid-liquid phase separation mechanism, resulting in the formation of microstructures. Very interestingly, the porosity of PLLA/ZnO@KH570 composite is highest up to 94.1% compared to its value of 87.8% for pure PLLA. Due to the multiple structures, the water contact angle of composite is significantly improved to 146°, allowing the oil/water mixture solution to be effectively separated. In brief, we have successfully manufactured hydrophobic PLLA/ZnO@KH570 composite with the multi-scale microstructure which may be potentially applied in oil-water separation.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Yanling Sun,Yanan Mao,Xin He,Xinghua Zhao
The aim of this study was to prepare polymeric micelles composed of enrofloxacin (ENR) and methoxy poly (ethylene glycol)-poly(lactide) (mPEG-PLLA) using a solvent evaporation method to overcome the solubility-limited oral bioavailability of ENR. The formulation was optimized using a Box–Behnken design (BBD) to obtain ENR polymeric micelles (ENR-m) with high drug loading (DL, %) and entrapment efficiency (EE, %). The physicochemical properties, in vitro drug release, pharmacokinetics, and antibacterial efficacy were evaluated in comparison to pure ENR. ENR-m was successfully prepared and demonstrated satisfactory drug loading (68.38 ± 0.22%), entrapment efficiency (88.40 ± 0.91%), particle size (PS) (133.67 ± 3.10 nm), and polydispersity index (PDI) (0.13 ± 0.03). The ENR-m also exhibited excellent stability under environmental conditions (40°C and 75% relative humidity (RH)). In vitro release of ENR from micelles was accelerated in a PBS solution. A pharmacokinetic study on beagles revealed that the oral bioavailability of ENR-m was enhanced by approximately 1.60-fold compared to pure ENR (p < 0.01) and by 1.66-fold compared to commercially available tablets of ENR (p < 0.01). The antibacterial activity of ENR-m against Escherichia coli (E. coli) and Salmonella typhi (S. typhi) was stronger than that of pure ENR.
研究领域:
药剂学
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药理学
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微生物学
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高分子科学
·
药物传递
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Bo Li,Haocheng Yang,Hongli Song,Jie Zou,Chenchen Li,Ziyi Yan,Moyuan Qu,Wenqian Xiao,Songsong Zhu,Xiaoling Liao
Repairing bone defects is particularly challenging when an infection is presented. In this study, poly(L-lactic) (PLLA) nanofibrous microspheres (large microspheres) were used as cell carriers to simulate the nanofibrous structure of the extracellular matrix. A planet-satellite composite microsphere with large microspheres and small microspheres was constructed by bonding hollow Ag@SiO 2 nanospheres (small microspheres) to nanofibrous PLLA microspheres. According to the results, PLLA nanofibrous microspheres activated by EDC and NHS can firmly combine with Ag@SiO 2 nanospheres. The composite microspheres demonstrated excellent drug release functions, antibacterial properties, and biocompatibility and promoted cell adhesion and differentiation in vitro. The bone repair function of composite microspheres loaded with BMP2 growth factor was evaluated in rat that were infected with bacteria and exhibited skull defects. A four-week study showed that these microspheres significantly promoted bone regeneration in infected bone. These multifunctional microspheres could revolutionize bone repair and provide a much-needed solution for those suffering from infected bone defects.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
骨科
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Qiuxiang Li,Guowei Zhao,Fengling Wu,Xilan Tang,Zhenggen Liao,Xinli Liang,Wei Dong
Imperatorin (IMP) has demonstrated potential in reversing drug resistance in various tumor cells; however, its limited clinical application and development are attributed to its poor water solubility and low bioavailability. The current work is intended to enhance the oral bioavailability of IMP and its efficacy in overcoming tamoxifen (TAM) resistance in breast cancer cells. We employed mPEG–PLLA and Cremophor RH60 (RH60) to prepare imperatorin nanomicelles (IMP-RH60/mPEG–PLLA). The freeze-dried powder of IMP-RH60/mPEG–PLLA demonstrated a porous structure, and upon reconstitution, the resulting solution exhibited clarity and transparency. The particle size was measured to be 25.48 ± 0.92 nm, with a zeta potential of −5.50 ± 1.06 mV. The encapsulation efficiency was determined to be 85.78 ± 0.84%, while the drug loading was found to be 2.66 ± 0.03%. In comparison to the active pharmaceutical ingredients (APIs) of IMP, IMP-RH60/mPEG–PLLA exhibited a significant increase in in vitro release, improved bioavailability in rats, and enhanced uptake by MCF-7/TAM cells. The coadministration of IMP-RH60/mPEG–PLLA and TAM effectively inhibited the proliferation, colony formation, migration, and invasion of MCF-7/TAM cells. This outcome could potentially be attributed to the downregulation of GST-π and MDR1 drug resistance genes, consequently augmenting the susceptibility of MCF-7/TAM cells to TAM. An experimental model of BALB/c nude xenograft, consisting of human breast cancer cells resistant to tamoxifen (TAM), was established in order to assess the potential in vivo reversal of TAM resistance through the use of IMP-RH60/mPEG–PLLA. The growth of xenografts was inhibited by IMP-RH60/mPEG–PLLA, and it induced apoptosis in tumor tissues through the downregulation of Ki-67, GST-π, and MDR1 gene expression. The findings indicated that the utilization of IMP-RH60/mPEG–PLLA micelles resulted in an enhancement of IMP’s bioavailability and its efficacy in overcoming TAM resistance.
研究领域:
药学
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肿瘤学
·
纳米技术
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Zhu Xiong,Yun Zhong,Haibo Lin,Fu Liu,Tiantian Li,Jinglong Li
In this work, a feasible and efficient approach was developed to produce Poly(lactic acid (PLA) ultrafiltration membrane with both high permeability and high rejection simultaneously. Poly(L-lactic acid)/Poly(D-lactic acid) (PDLA/PLLA) stereocomplex crystallites were thought to play the critical role different from the traditional pore forming agents or hydrophilic modification. The membrane morphology of the stereocomplex PDLA/PLLA membrane with high porosity and thin skin layer was observed by SEM. The addition of PDLA resulted in the thermodynamic enhancement for phase separation of PLLA casting solution as demonstrated by a rotational rheometer. The PDLA/PLLA stereocomplex crystallization behavior was investigated by DSC and XRD . With increasing the stereocomplex crystallites, the water permeability of PLA membrane significantly increased from ~482 L/m 2 ·h to ~3325 L/m 2 h, while with small loss in degree of rejection. Furthermore, the stereocomplex PLA membrane demonstrated the excellent separation efficiency for nano-TiO 2 and ink dispersion. Besides, the stereocomplex PLA membrane exhibited outstanding anti-protein fouling properties through the static and dynamic BSA antifouling tests. All results demonstrated that the stereocomplexation can effectively tune the pore size evolution and control the permeability, selectivity and fouling resistance.
研究领域:
材料科学
·
膜技术
·
高分子化学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2021
·
IF 4.598
Yajun Ren,ZhuanZhuan Zhang,Wenlong Xia,Qifeng Zhou,Xiaofeng Song
Poly(L-lactide) (PLLA) was one of the most well-known biodegradable polyesters. Although its thermo-responsive shape memory (SM) function has been studied by proper program, high transformation temperature is not suitable in some biomedical applications. Water-responsive SM polymer is an alternative choice and exhibits great potential because it is harmless to the cells and tissues of organisms. In the work, a novel PCL-(PMVS- s -PAA)-PCL-PTMG-PCL-(PMVS- s -PAA)-PCL copolymer was devised as an additive to endow PLLA with water-responsive SM capability. The incorporated PLLA was characterized by cross-section, water contact angle, differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA). Inserted PTMG segment and assisted ambient temperature were contributed to its SM behaviors. The shape fixation ratio and shape recovery ratio were 92.0% and 94.2%, respectively. The schematic diagram between internal microstructures and macroscopic properties was proposed. At the same time, the incorporated PLLA showed excellent toughness. So the obtained SM material would be promising for medical and biomimetic fields.
研究领域:
材料科学
·
生物医学工程
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 39
·9110-9120
·
IF 5.344
Zhiyang Xu,Yulong Xu,Papia Basuthakur,Chitta Ranjan Patra,Seeram Ramakrishna,Yong Liu,Vinoy Thomas,Himansu Sekhar Nanda
Functionalized cerium oxide nanoparticle (CeNP)-loaded fibro-porous poly-L-lactic acid (PLLA)/gelatin composite membranes were prepared via an electrospinning technology. Considering the importance of such membrane scaffolds for promoting angiogenesis in tissue engineering and drug screening, a series of PLLA/gelatin composite fiber membranes loaded with different doses of CeNPs was prepared. The prepared composite membranes demonstrated hydrophilicity, water absorption, and improved mechanical properties compared to a PLLA and PLLA/gelatin membrane. Also, cell viability assay using somatic hybrid endothelial cells (EA.hy926) proved the biocompatible nature of the scaffolds. The biocompatibility was further supported by in vivo chick embryo angiogenesis assay using fertilized eggs. Our initial results support that these membrane scaffolds could be useful for angiogenesis-related disease treatment after further investigations.
研究领域:
组织工程
·
材料科学
·
生物材料
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2023
·
IF 13.3
Zuyun Yan,Tianshi Sun,Wei Tan,Zhicheng Wang,Jinpeng Yan,Jinglei Miao,Xin Wu,Pei Feng,Youwen Deng
In the system of magnesium-loaded scaffolds, the effect of magnesium ions (Mg 2+ ) on the osteogenesis induction is restricted due to the low transmembrane transport efficiency of Mg 2+ into the cell, which limits the application for bone defect repair. Inspired by the fact that magnetic field can regulate ion channel proteins on the cell membrane, magnetite nanoparticle is introduced into the poly (l-lactic acid) /magnesium oxide composite in this study, and a magnetic magnesium-loaded bone scaffold is prepared via selective laser sintering . Notably, the activities of the Mg 2+ channel protein (MAGT1) on the membrane of bone marrow mesenchymal stem cells (rBMSCs) are enhanced via magnetic torque effect (via integrin α V β 3/actin), under the action of static magnetic field (SMF), which promoted rBMSCs to capture Mg 2+ in the microenvironment and induced osteogenesis. In vitro experiments showed that the magnetic magnesium-loaded scaffold, under the action of SMF, can accelerate the inflow of Mg 2+ from surrounding microenvironment, which improved cellular activities, osteogenesis-related gene expression (ALP, Runx2, OCN, and OPN), and mineralization. Besides, in vivo skull defect repair experiments showed that the scaffolds possessed good ability to promote bone differentiation and new bone regeneration.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
细胞生物学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Hai Jiang,Yingli Ding,Juyang Liu,Arun Alagarsamy,Li Pan,Dongpo Song,Kunyu Zhang,Yuesheng Li
A series of poly(L-lactic acid) (PLLA)-b-poly(methyl methacrylate) (PMMA) block copolymers with well-defined chemical structures were synthesized by ring-opening polymerization followed by atom-transfer radical polymerization. These copolymers were investigated as effective A-b-C-type compatibilizers for poly(lactic acid) (PLA) and renewable poly(epichlorohydrin-co-ethylene oxide) (ECO) elastomer blends. Compared to the neat binary PLA/ECO blend, the PLLA-b-PMMA copolymers significantly improved the compatibility of the PLA and ECO phases, leading to enhanced interfacial adhesion and mechanical performance. Interestingly, by tuning the chain structure and adding different amounts of the copolymer, two different phase structures were achieved in the blends: a typical sea-island morphology of the blends with a low content of PLLA-b-PMMA block copolymers (<15%) and a unique tricontinuous phase morphology with a percolated PLLA-b-PMMA copolymer zone of the blend with the addition of 20 wt % asymmetric A181M868 block copolymer. Both the morphological structures endowed the blends with excellent toughness, including a high elongation at break (>260%) and non-broken behavior with an optimum impact strength above 63 kJ/m2. Accordingly, two different toughening mechanisms were proposed for the blends with different phase structures. The PLLA-b-PMMA block copolymers provide highly efficient and versatile A-b-C-type compatibilizers to fabricate high-performance sustainable PLA-based materials for wide application prospects.
研究领域:
材料科学
·
高分子科学
·
可持续材料
·
复合材料
✓ 已验证
DOI
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2023
·
IF 9.1
Xin Yang,Fuqing Wei,Zhuoni Wang,Guangquan Li,Shiyuan Yang,Jiachun Feng
While nanoparticles were considered to be the most promising compatibilizers for immiscible polymer blends , high-efficient nanoparticle-based compatibilizers were still under development. Herein, a high-reactive silica nanosheet (HRN) compatibilizer with excellent compatibilization effect was synthesized and its compatibilization effect was investigated. The HRN compatibilizer was fabricated by skillfully melt-blending amino functionalized silica hollow spheres (AHS) and the poly(styrene-co-glycidyl methacrylate) (PSGMA) with average ten reactive epoxy groups per chain. During the mixing, the AHS were crushed to nanosheets upon the strong shearing and simultaneously grafted PSGMA chains on nanosheets via the reaction of epoxy and amino groups. The PSGMA with high epoxy groups content endowed the HRN with high reactivity. To demonstrate the compatibilization efficiency of our high-reactive compatibilizer, different amounts of HRN were incorporated into the immiscible polylactic (l-acid)/polybutylene adipate terephthalate (PLLA/PBAT) blend, a blend system widely applied in packing and agricultural films, to investigate and compare the changes in morphologies and mechanical properties . Only 3 wt% addition of HRN compatibilizer could significantly decrease the droplets size and dramatically improve the tensile strength and elongation at break of the blend by 1.4 and 23 times. The present work provided a new idea for the development of nanoparticle-based compatibilizer with high compatibilization efficiency.
研究领域:
材料科学
·
高分子科学
·
纳米技术
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Donglei Liu,Jiaxin Han,Xijing Zhuang,Yang Gao,Wenjun Wang,Dan Qin,Xufeng Dong
A three-layer small-diameter vascular graft (SDVG) of polycaprolactone (PCL) and l -polylactic acid (PLLA) with pore diameter gradient was prepared through combining variable-extrusion speed iterative electrospinning and ultrasonic reaming. The SEM results showed that the three-layer PCL/PLLA SDVG has distinct pore diameter gradient. The Young’s modulus, ultimate tensile strength (UTS), burst pressure (BP) and compliance of the vascular graft were high as 20.116 ± 0.465 MPa, 2.259 ± 0.141 MPa, 2050 ± 249 mmHg and 4.203 ± 0.330 %/100 mmHg, respectively. The UTS of the graft is similar to human coronary arteries. The BP meets the basic requirements to prevent aneurysmal dilation, and the compliance is higher than that of sheep carotid artery. The fluid–solid coupling simulation results showed that the graft had high mechanical properties to withstand the same blood flow velocity as human coronary arteries, and there was no stress concentration in the wall. Besides, it had good biocompatibility, promoting the adhesion and proliferation of rat vascular endothelial cells (ECs). The successful preparation of the small-diameter vascular graft with high mechanical properties for preventing itself from rupturing and ensuring the overall function, provides more possibilities for the clinical transplantation of small-diameter vascular grafts.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）