ENVIRONMENTAL RESEARCH
·
2026
·
IF 8.2
Tong Wang,Xinyuan Wang,Xiao Wei,Mengwei Yan,Sihang Gao,Wenjuan Yang
Efficient sequestration of oxytetracycline (OTC) from aqueous environments is limited by the single interaction force of conventional adsorbents, stemming fundamentally from the insufficient understanding and translation of adsorption mechanisms into rational material design. In this study, based on the molecular structure complexity of OTC, a new type of waste-derived macroscopic adsorbent with high adsorption capacity was developed from the perspective of multi-site and multiple intermolecular forces synergy design. A covalent organic frameworks (COFs) rich in secondary amine, ketone, and sulfonic acid groups was grown in situ on peony pod-based cellulose-sodium alginate composite aerogel (PCSA). The composite PCSA@COFs showed an effective OTC adsorption capacity of 43.44 mg/g at 298 K, following pseudo-second-order and Langmuir models, indicating spontaneous, endothermic chemisorption. Density functional theory calculations further revealed a strong binding energy of −55.11 kcal/mol between COFs and OTC with multiple interaction sites. Topological analysis via Atoms in Molecules theory demonstrated a multi-site three-dimensional synergistic mechanism through the identification of ring critical points and cage critical points, while the topological parameters revealed a partially covalent character in the interaction between sulfonic acid groups and OTC. This work provides a novel design concept for developing effective and eco-friendly adsorbents for antibiotic removal from aqueous environments.
✓ 已验证
产品关联
DOI
高影响因子
FOOD SCIENCE AND TECHNOLOGY RESEARCH
·
2021
·
IF 0.668
Shirong Huang,Huan Liu,Sinian Yan,Dongfang Chen,Xin Mei
Here, the changes in phenolic composition and antioxidant activities (ABTS, DPPH, and FRAP) of raw and pickled cowpea green pod during in vitro simulated gastrointestinal digestion (GID) were investigated. The inhibitory activities of the undigested and digested samples towards α-amylase, α-glucosidase and lipase were determined. Results revealed that total phenolic (TP) and flavonoid (TF) contents of both raw and pickled samples were increased after gastric and intestinal digestion, and the pickled sample had lower TP and TF content after each digestion phase. A similar trend was observed for antioxidant activities, which were significantly correlated with TP and TF contents (p < 0.05). Seven phenolic compounds were identified and quantified by HPLC-DAD. Their contents were affected by digestion and pickling, and raw cowpea pod had higher contents before and after GID. Inhibitory effects towards α-amylase, α-glucosidase and lipase were increased for raw cowpea pod after GID. Pickled cowpea pod also showed an increased inhibitory activity towards lipase, while no activity towards α-amylase and α-glucosidase. Raw cowpea pod had higher inhibitory effects towards α-amylase, α-glucosidase and lipase than the pickled one at each digestion phase. Our results showed that pickling process reduced the health-promoting properties of cowpea green pod.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2015
·Vol. 10
·期 10
·e0141208
·
IF 3.234
Jing Zhou,Na Zhang,Pan Wang,Shichang Zhang,Daiqin Li,Kaiyu Liu,Guoxiu Wang,Xiaoping Wang,Hui Ai
Chemoreception is a key feature in selection of host plant by phytophagous insects, and odorant-binding proteins (OBPs) are involved in chemical communication of both insects and vertebrates. The legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae) is one of the key pest species of cowpea and widely distributed throughout tropical and subtropical regions, causing up to 80% of yield loss. In this study, we investigated the electrophysiological responses of female M . vitrata to floral volatiles from V . unguiculata . Seventeen electroantennogram-active compounds were identified from floral volatiles of V . unguiculata by coupled gas chromatography-electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). Then, we cloned two novel full-length GOBP genes ( MvitGOBP1 and MvitGOBP2 ) from the antennae of M . vitrata using reverse transcription PCR. Protein sequence analysis indicated that they shared high sequence similarity with other Pyralididae insect GOBPs and had the typical six-cysteine signature. Real-time PCR analysis indicated that MvitGOBP1-2 mRNA was highly expressed in the antennae of female adult with several thousands-fold difference compare to other tissue. Next, the recombinant MvitGOBP1-2 was expressed in Escherichia coli and purified using Ni ion affinity chromatography. Fluorescence binding assays demonstrated that MvitGOBP1-2 had different binding affinities with 17 volatile odorant molecules including butanoic acid butyl ester, limonene, 4-ethylpropiophenone, 1H-indol-4-ol, butanoic acid octyl ester and 2-methyl-3-phenylpropanal. In the field trapping experiment, these six floral volatiles could effectively attract female moths and showed significant difference compared with the blank lure. These results suggested that MvitGOBPs and the seventeen floral volatiles are likely to function in the olfactory behavior response of female moths, which may have played crucial roles in the selection of oviposition sites. The six compounds that we have identified from the volatiles of V . unguiculata may provide useful information for exploring efficiency monitoring and integrated pest management strategies of this legume pod borer in the field.
研究领域:
昆虫学
·
化学生态学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yijie Wang,Zichong Ye,Jiahui Li,Yan Zhang,Yingxi Guo,Jun-Hu Cheng
Litchi is one kind of the precious tropical fruits, but it is easily affected by enzymatic browning resulting in great loss to farmers. In the present study, dielectric barrier discharge cold plasma was used to inactivate the litchi peroxidase (POD), one of the enzymes responsible for browning. Results indicated that after 10 min treatment, the residual activity of the POD extracted from pericarp decreased to 47.16%. The effect of cold plasma on POD structure was studied by using horseradish peroxidase (HRP) as a model enzyme. The HRP activity was decreased by about 85% after 180 s of treatment. UV and fluorescence spectral results suggested that the secondary and tertiary structures of HRP were changed through plasma treatment, hydrophobic aromatic amino acids and hemes were exposed and modified. Circular dichroism (CD) spectra indicated that after exposed to plasma for 180 s, the content of α-helix in HRP were decreased from 21.41% to 8.53%, and the β-sheet content raised from 23.29% to 32.11%. The presented study concluded that cold plasma is capable of reducing the activity of litchi POD, which will provide a new thought for prolonging shelf life of easy-browning fruits.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Terefe Tafese Bezuneh,Fuchun Nan,Xin Li,Tongtong Kou,Zilong Wu,Fangfang Lang,William W. Yu
Designing highly sensitive method for the analysis of α-glucosidase activity is of critical value for diagnosis and therapy of diabetes. Here, we employed self-assembly of guanosine 5′-monophosphate nucleotide (GMP) with metal ions (Cu 2+ and Tb 3+ ) to prepare multifunctional Tb-CuGMP NPs. Owing to Cu 2+ -catalytic center, the as-prepared Tb-CuGMP NPs exhibits ascorbic acid oxidase (AAO)-like and peroxidase (POD)-like self-cascading catalytic activity. The AAO-POD cascade system can catalyze the oxidation of AA into dehydroascorbic acid (DHAA) with the generation of H 2 O 2 , which serves as a substrate for POD-like activity and catalyzed into • OH radicals. Based on these enzymatic reactions, a unique colorimetric detection approach in which nanozyme/TMB/AA produced oxidized TMB with characteristic blue color and DHAA sensitized Tb 3+ to turn-on the fluorescence of the detection system was proposed. Furthermore, enzymatic hydrolysis of 2-O-a-D-glucopyranosyl-L-ascorbic acid (AA2G) into AA was coupled with AAO-POD cascade system to realize highly sensitive detection of α-glucosidase activities. Interestingly, the as-constructed dual-mode assay reduces the LOD of α-glucosidase to 5.1 × 10 −4 U/mL. To sum up, this work provides new horizon for the development of efficient multifunctional nanomaterial with integration of biocatalysis and bioassay into a single material.
研究领域:
纳米技术
·
酶活性分析
·
生物传感
·
生物检测
·
催化
·
糖尿病诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiaxin Dong,Guanxiong Liu,Yuri V. Petrov,Yujie Feng,Dechang Jia,Vladimir E Baulin,Aslan Yu Tsivadze,Yu Zhou,Baoqiang Li
Iron phosphide/carbon (FeP/C) serving as electrocatalysts exhibit excellent activity in oxygen reduction reaction (ORR) process. H 2 O 2 catalyzed by peroxidase (POD) is similar to the formation of new electron transfer channels and the optimization of adsorption of oxygen-containing intermediates or desorption of products in ORR process. However, it is still a challenge to discover FeP/C with enhanced POD-like catalytic activity in the electrocatalytic database for biocatalysis. The discovery of FeP/carbon dots (FeP/CDs) nanozymes driven by electrocatalytic activity for enhanced POD-like ability is demonstrated. FeP/CDs derived from CDs-Fe 3+ chelates show enhanced POD-like catalytic and antibacterial activity. FeP/CDs exhibit enhanced POD-like activities with a specific activity of 31.1 U mg −1 that is double higher than that of FeP. The antibacterial ability of FeP/CDs nanozymes with enhanced POD-like activity is 98.1%. The antibacterial rate of FeP/CDs nanozymes (250 µg mL −1 ) increased by 5%, 15%, and 36% compared with FeP, Fe 2 O 3 /CDs, and Cu 3 P/CDs nanozymes, respectively. FeP/CDs nanozymes will attract more efforts to discover or screen transition metal phosphide/C nanozymes with enhanced POD-like catalytic activity for biocatalysis in the electrocatalytic database.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
催化
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Di Liu,Si Sun,Huanhuan Qiao,Qi Xin,Sufei Zhou,Lingxia Li,Nan Song,Lijie Zhang,Qi Chen,Fangzhen Tian,Xiaoyu Mu,Shaofang Zhang,Jing Zhang,Meili Guo,Hao Wang,Xiao-Dong Zhang,Ruiping Zhang
Artificial enzymes, especially nanozymes, have attracted wide attention due to their controlled catalytic activity, selectivity, and stability. The rising Cerium-based nanozymes exhibit unique SOD-like activity, and Vanadium-based nanozymes always hold excellent GPx-like activity. However, most inflammatory diseases involve polymerase biocatalytic processes that require multi-enzyme activities. The nanocomposite can fulfill multi-enzymatic activity simultaneously, but large nanoparticles (>10 nm) cannot be excreted rapidly, leading to biosafety challenges. Herein, atomically precise Ce 12 V 6 clusters with a size of 2.19 nm are constructed. The Ce 12 V 6 clusters show excellent glutathione peroxidase (GPx) -like activity with a significantly lower Michaelis-Menten constant (K m , 0.0125 mM versus 0.03 mM of natural counterpart) and good activities mimic superoxide dismutase (SOD) and peroxidase (POD). The Ce 12 V 6 clusters exhibit the ability to scavenge the ROS including O 2 ·− and H 2 O 2 via the cascade reactions of multi-enzymatic activities. Further, the Ce 12 V 6 clusters modulate the proinflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) and consequently rescue the multi-organ failure in the lipopolysaccharide (LPS)-induced sepsis mouse model. With excellent biocompatibility, the Ce 12 V 6 clusters show promise in the treatment of sepsis.
研究领域:
纳米技术
·
生物化学
·
药理学
·
免疫学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2022
·Vol. 18
·期 20
·2200178
·
IF 15.153
Lunjie Huang,Da-Wen Sun,Hongbin Pu
Metal-organic frameworks (MOFs) are a versatile toolbox for the bioinspired design of nanozymes for antibacterial applications and beyond, however, designing a nanozyme by the hierarchical quasi-MOF scheme remains largely unpracticed. This work exemplifies the preferential structure-activity correlation of a bimetallic quasi-MOF (Q-MOF Ce0.5 ) among three series of MOF-derived peroxidase (POD) mimics. The biomimetic quasi-MOF Ce0.5 nanosheets accommodate both oxygen vacancy-coupled multivalent redox cycles and photosensitive energy band layout, benefiting from the hierarchical heterojunction-like 0D/2D interface featuring isolated nodes-derived Ce O Cu sites upon the 2D decarboxylated MOF scaffold. These integrated unique merits enable the POD-like Q-MOF Ce0.5 to generate sustained reactive oxygen species to effectively eradicate the surface-adhered bacteria under visible light, resulting in significant inactivation of Escherichia coli (99.74 %) and Staphylococcus aureus (99.35%) in vitro, and potent disinfection of skin wounds in vivo in safe and on-demand manners. It is hoped that this work can intensify the interventions of MOF nanozymes against the microbial world.
研究领域:
纳米技术
·
生物催化
·
抗菌材料
·
材料科学
·
生物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-15
·
IF 14.7
Jiang Peng,Zhang Ludan,Liu Xiaolong,Ye Chenliang,Zhu Peng,Tan Ting,Wang Dingsheng,Wang Yuguang
The reaction system of hydrogen peroxide (H 2 O 2 ) catalyzed by nanozyme has a broad prospect in antibacterial treatment. However, the complex catalytic activities of nanozymes lead to multiple pathways reacting in parallel, causing uncertain antibacterial results. New approach to effectively regulate the multiple catalytic activities of nanozyme is in urgent need. Herein, Cu single site is modified on nanoceria with various catalytic activities, such as peroxidase-like activity (POD) and hydroxyl radical antioxidant capacity (HORAC). Benefiting from the interaction between coordinated Cu and CeO 2 substrate, POD is enhanced while HORAC is inhibited, which is further confirmed by density functional theory (DFT) calculations. Cu-CeO 2 + H 2 O 2 system shows good antibacterial properties both in vitro and in vivo. In this work, the strategy based on the interaction between coordinated metal and carrier provides a general clue for optimizing the complex activities of nanozymes.
研究领域:
材料科学
·
纳米技术
·
催化
·
生物医学工程
·
抗菌研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Na Li,Jing Tang,Congxiao Wang,Minghui Wang,Guodong Chen,Lei Jiao,Qinglai Yang,Xiaofeng Tan
The rational design of nanozymes with highly efficient reactive oxygen species (ROS) generation to overcome the resistant infection microenvironment still faces a significant challenge. Herein, the highly active Fe single-atom nanozymes (Fe SAzymes) with a hierarchically porous nanostructure were prepared through a colloidal silica-induced template method. The proposed Fe SAzymes with satisfactory oxidase (OD)-like and peroxidase (POD)-like activity can transform O 2 and H 2 O 2 to superoxide anion free radical (•O 2 - ) and hydroxyl radical (•OH), which possess an excellent bactericidal effect. Also, the glutathione peroxidase (GPX)-like activity of Fe SAzymes can consume glutathione in the infection microenvironment, thus facilitating ROS generation to enhance the sterilization effect. Besides, the intrinsic photothermal effect of Fe SAzymes further significantly boosts the enzyme-like activity to generate much more reactive oxygen species for efficient antibacterial therapy. Accordingly, both in vitro and in vivo results indicate that the Fe SAzymes with synergistically photothermal-catalytic performances exhibit satisfactory antibacterial effects and biocompatibility. This work provides new insights into designing highly efficient SAzymes for effective sterilization applications by an amount of ROS generation.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2020
·
IF 2.751
Xiang Zhou,Yiqiang Ye,Shasha Liu,Wubin Shao,Liwei Liu,Song Yang,Zhibing Wu
Plant viral diseases, known as “plant cancer”, with high contagiosity can substantially reduce crop quality and yield. To identify potential anti- tobacco mosaic virus (TMV) agents with different mechanisms, a series of novel α-aminophosphonate derivatives containing a chalcone moiety were designed and synthesized. Bioassay results revealed that some target compounds exhibited improved curative activity against TMV in vivo, and the EC 50 value of compound B 3 was 356.7 mg L −1 . The activities of the defensive enzymes POD and CAT from tobacco leaves treated with B 3 and B 17 showed that these target compounds could improve the photosynthetic ability of the leaves and activate plant host resistance against TMV infection. The binding constant between B 3 and TMV Coat Protein (CP) (2.51 × 10 8 M −1 ), calculated by the fluorescence titration experiment and docking results, revealed that B 3 has a strong interaction with TMV CP. Further docking analysis revealed that B 3 was embedded between two layers of the TMV CP, which was consistent with the 2:1 binding mode of TMV CP and B 3 determined by the binding affinity experiment. The TEM morphological study of TMV treated with B 3 and B 17 indicated that this series of target compounds may trigger the disassembly of TMV by interacting directly with TMV CP. This study provides new insight for the discovery of antiviral compounds with two different mechanisms of action.
研究领域:
植物病毒学
·
有机化学
·
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 14
·期 2
·361-372
·
IF 7.79
Ruoxi Zhao,Rui Zhang,Lili Feng,Yushan Dong,Jialing Zhou,Songnan Qu,Shili Gai,Dan Yang,He Ding,Piaoping Yang
The construction of nanoplatforms with combined photothermal properties and cascading enzymatic activities has become an active area of anticancer research. However, the overheating of photothermal therapy (PTT) and the specific properties of tumor microenvironment (TME) greatly impaired the therapeutic efficiency. Herein, we rationally fabricated a virus-like SiOx/CeO2/VOx (SCV) nanoplatform for 1064 nm near-infrared (NIR) triggered mild-temperature PTT and nanozyme catalytic therapy. Firstly, the virus-like shape of SiOx/CeO2/VOx made it favorable for cell adhesion and improved its phagocytosis in cells, and the SCV generated an effective PTT effect upon 1064 nm laser irradiation. Particularly, the produced VO2+ in TME could be used as a heat shock protein inhibitor to inhibit the expression of heat shock protein 60 (HSP60) to enhance the PTT efficiency. Moreover, the SCV nanozyme exhibited obvious peroxidase-mimic (POD) catalytic activity, which could generate highly toxic free radical ions (˙OH) under acidic conditions. The mild-temperature heat and ˙OH produced by enzymatic catalysis effectively blocked the tumor growth, as verified firmly by in vitro and in vivo tests. Our designed virus-like SCV nanozyme with POD mimic enzyme activity and a mild photothermal effect may provide a new way of thinking about the combination therapy model.
研究领域:
纳米医学
·
癌症治疗
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）