Drug Delivery and Translational Research
·
2026
·
IF 6.9
Cao Ruijie,Zhou Rongwei,Wang Chuancui,Shen Meizhu,Yu Jiangyue,Xu Hao,Guo Zhong
Acute lung injury (ALI) is characterized by uncontrolled inflammation and oxidative stress, driven largely by macrophage dysregulation. Despite their anti-inflammatory potential, flavonoids like quercetin (Qu) are limited by poor solubility and systemic toxicity. To address these challenges, this study developed a “bioactive co-assembly” nanomicelle platform (Qu@PSA-VES/diT-VES) based on polysialic acid (PSA) and a novel dimerized taurine-vitamin E succinate (diT-VES). Molecular docking simulations demonstrated that quercetin (Qu) exhibits an exceptionally high binding affinity for the VES hydrophobic core. Furthermore, the incorporation of diT-VES significantly enhanced the colloidal stability of the micelles through strengthened non-covalent interactions, effectively preventing disassembly during physiological circulation. Hydrophobic interactions and hydrogen bonding were identified as the primary driving forces for micellar stability. The carrier leverages PSA to specifically target the overexpressed Siglec-1 receptor on the surface of inflammatory macrophages, thereby mediating receptor-dependent endocytosis. Within the acidic and enzyme-enriched lysosomal environment, the micelles undergo pH/enzyme dual-responsive dissociation, facilitating the escape of the drug from the lysosomal barrier and its subsequent diffusion into the cytoplasm for pharmacological action. Additionally, the carrier components VES and taurine provide antioxidant and mitochondrial protection, respectively, synergizing with Qu to significantly induce the reprogramming of M1 macrophages toward the M2 phenotype in vitro. In a murine ALI model, the system demonstrated superior lung-targeting ability, significantly reducing the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in bronchoalveolar lavage fluid and alleviating pulmonary edema and neutrophil infiltration. Experimental results indicated that under a lethal ALI challenge, the 72-h survival rate of mice in the treatment group was significantly increased from 16.7% to 83.3%, while maintaining excellent in vivo biocompatibility. This integrated "targeting-stabilization-synergy" nanoplatform provides a promising translational strategy for the treatment of macrophage-driven inflammatory disorders.
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Jia Hui He,Yun Ying Cheng,Qian Qian Zhang,Hui Liu,Cheng Zhi Huang
The accurate and sensitive detection of biomarkers has great clinical value for the early diagnosis and treatment of cancer. Due to the excellent optical properties of carbon dots (CDs), CDs-based fluorescent detection methods have attracted increasing attention in bioanalytics. Signal reporters using CDs labeled hairpin DNA , based on Föster resonance energy transfer (FRET), have shown promise for the sensitive detection of biomarkers. In this work, a new method for sensitive biomarker detection using an enzyme-free amplified fluorescence strategy was developed. The strategy was based on FRET between CDs and graphene oxide (GO) combined with catalytic hairpin assembly (CHA). In the absence of the target, the CD-labeled hairpin DNA adsorb onto GO via hydrophobic and π-π stacking interactions, resulting in a FRET quenching of the CDs fluorescence. The introduction of the target could trigger the CHA circuits to form Y-shaped double-stranded DNA (dsDNA), resulting in a recovery of the CD's fluorescence signal. This novel strategy was successfully applied for the selective detection of prostate specific antigen (PSA) with a limit of detection (LOD) of 0.22 ng mL −1 (3σ/k). Additionally, the method could be used to detect carcinoembryonic antigen (CEA) and adenosine triphosphate (ATP) with LOD of 0.56 ng mL −1 (3σ/k) and 80 nM (3σ/k), respectively. Therefore, this work demonstrates a promising way to construct a sensitive and versatile detection platform.
研究领域:
生物分析学
·
医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 13
·10131-10141
·
IF 2.779
Lu Hao,Dong Yaqiang,Liu Xincai,Liu Zhonghao,Ma Yan,Wu Yue,He Aina,Li Jiawei,Wang Xinmin
The phosphating treatment method has been extensively applied in the field of insulating soft magnetic powders because of its simplicity and ease of operation; however, the specific nanoscale morphology of phosphate coating layers and the interface/connection/interaction with the outer resin are still unclear. In this study, soft magnetic powder cores (SMPCs) with a nanoporous-structured phosphate coating were fabricated, in which the coating has a beneficial adsorption effect on the outer resin and can enhance the bonding force between the organic/inorganic coating. Optimized soft magnetic properties were obtained for the SMPCs with a phosphoric acid content of 0.6 wt%, including a high effective permeability of 61, low core loss of 134.6 mW/cm 3 (measured at 100 mT, 50 kHz), and high DC bias performance of 56% under a superimposed field of 100 Oe due to the favorable interactions between the insulating coating layers. These properties can be used to manufacture micro-magnetic components.
研究领域:
材料科学
·
电磁学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2023
·
IF 4.6
Xia Zhao,Hong Hao,Haixia Pang,Min Jiang,Yanping Duan
Emulsifier-free polymerization was employed to prepare the submicron spherical PSA (the copolymer of styrene and acrylic acid), then SiO 2 was grown on the surface of the PSA by sol-gel method to form F–SiO 2 @PSA particles. It was found that when styrene: acrylic acid = 2.5:1 and tetraethyl orthosilicate : PSA was between 2.17:1 and 6.38:1, a perfect raspberry-like structure could be obtained. The coating based on F–SiO 2 @PSA particles exhibited contact angles of 167.3 ± 1.4°, 152.1 ± 1.5°,150.3 ± 1.6°, 150.9 ± 1.6°, 142.2 ± 1.7°, 143.7 ± 1.5° and 146.6 ± 1.9° to water, diiodomethane, soybean oil, rapeseed oil, hexadecane, kerosene, and waste engine oil , respectively. The F–SiO 2 @PSA coating could reduce the adsorption of protein by 90% compared with the control surface . It was observed that bacteria and platelets could hardly adhere to the coating surface after 24 h of E. coli culture and 3 h of platelet-rich plasma immersion. The anti-adhesion to proteins, platelets, and bacteria will give the coating a promising application in the medical field.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yanpei Xu,Silong Li,Shuxian Gao,Fudong Jia,Liang Guo,Jiangrong Hou,Shaowei Ma,Heya Zheng,Chuxiao Sun,Qi Wang
Prostate-specific antigen (PSA) detection is vital for early prostate cancer diagnosis, yet conventional methods suffer from sensitivity and label-dependent limitations. This study presents a surface plasmon resonance (SPR) biosensor enhanced by Ni(OH) 2 /Ti 3 C 2 T x MXene nanocomposites (NTC) for sensitive detection of total PSA (tPSA) in serum. The NTC architecture synergizes carboxyl-functionalized MXene, amplifying localized electric fields and antibody-binding sites, with Ni(OH) 2 nanoflowers that prevent MXene stacking and enhance refractive index (RI) sensitivity. Optimized via finite element modeling (50-nm Au film, 72.4 ° incident angle), the Au-NTC architecture achieved a 39.34% improvement in RI sensitivity (3064.86 nm/RIU) compared to pristine Au. Through the dual signal amplification mechanism of synergistic enhancement by nanomaterials and sandwich method with gold nanoparticles, enabled detection of tPSA in 20% serum across a wide dynamic range (0.2–160 ng/mL), fully covering the clinical risk threshold (4–10 ng/mL). The platform demonstrated an ultralow limit of detection (LOD) of 0.0361 ng/mL (0.5157 pM) and high specificity against interferons. Kinetic analysis revealed strong antigen-antibody affinity (equilibrium dissociation constant K D = 4 . 26 × 1 0 − 10 M), while batch-producible sensor chips and integrated microfluidics underscored practical applicability. This work advances label-free SPR biosensing for precision prostate cancer diagnostics, offering significant potential in point-of-care testing and early intervention strategies.
研究领域:
肿瘤学
·
生物传感器
·
免疫学
·
分子诊断
·
转化医学
·
纳米医学
·
临床检验
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Dong Chen,Yilin Ma,Annan Yang,Liping Hu,Hanlin Zhou,Jun Xu,Shanze Chen,Dingmeng Nie,Weifeng Feng,Huaihong Cai,Yanguang Cong,Jiang Pi,Lang Rao,Xueqin Huang,Pinghua Sun,Haibo Zhou
Prostate specific antigen (PSA) is widely used in liquid biopsy of prostate cancer (PCa) but still faces challenges due to the poor specificity. Herein, this study reports a double-SERS satellite immunoassay, made of an Au–Ag dealloyed intra-nanogap nanoflower (Au–Ag DINF) with strong SERS signals and Au magnetic nanoparticles (AuMNPs) with magnetic capture and SERS amplification, for sensing multiple PSA (free PSA (fPSA), complexed PSA (cPSA) and [-2]proPSA (p2PSA)) toward potential PCa screening. Unlike the previous studies focus on the tPSA and fPSA/tPSA ratio (f/t PSA%), this work introduces a multiple PSA-mediated Prostate Health Index (PHI) assay with significantly increased the predictive accuracy and specificity of PCa, especially the patients with a tPSA level in the “diagnostic gray zone”. Practical analysis of clinical samples demonstrated the SERS immunoassay is capable of identifying the PCa subjects from healthy candidates ( N = 13), and monitoring PCa patients before and after surgery ( N = 5), as well as screening suspicious ones who are in the “gray zone” ( N = 10). Overall, benefiting from the PSA-responsive satellite immuno-nanoassemble strategy, dual-SERS magnification effect, and multiple PSA-based PHI assessment, this proposes bioassay held enormous potential for the early diagnosis, screening, monitoring, and prognosis of PCa.
研究领域:
医学诊断
·
纳米生物技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 17
·2963-2971
·
IF 4.872
Yongbei Zhao,Qi Wang,Jianxiu Li,Hongmin Ma,Yong Zhang,Dan Wu,Bin Du,Qin Wei
In this paper, amidogen graphene (NH2-Gr) and Au nanoparticle (NP) functionalized cerium oxide (CeO2) NPs (NH2-Gr/Au@CeO2) have been synthesized. The prepared NH2-Gr/Au@CeO2 exhibits strong electrochemiluminescence (ECL) activity which could be quenched efficiently by bismuth sulfide (Bi2S3). Based on this principle, a novel sandwich ECL immunosensor was constructed for the detection of prostate specific antigen (PSA). NH2-Gr was used as a loading matrix for CeO2 NPs because of its superior conductivity and a large surface area. Au NPs were used to combine anti-PSA 1 (Ab1) to CeO2via the Au–NH2 covalent bond and meanwhile to enhance the sensitivity of the immunosensor. NH2-Gr/Au@CeO2 was used as the ECL response layer. Ag NP functionalized Bi2S3 was used to label the secondary antibody (Ab2). After a sandwich-type immunoreaction, a remarkable decrease of the ECL signal was observed. The signal-off sensor showed a wide linear range response from 1 pg mL−1 to 10 ng mL−1 with a low detection limit (LOD) of 0.3 pg mL−1. The applicability of the proposed ECL immunosensor was evaluated by detecting PSA in human serum samples.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-12
·
IF 3.125
Zhao Xiaoyu,Zhang Yingbing,Zhang Jin,Xue Peijie,Wang Yanfei,Liu Rui,Cao Ruge,Zhu Liang,Li Gang,Sha Zuoliang
The highly dispersed silver nanoparticle-loaded poly(styrene-co-acrylic acid) nanocomposites (nAg@PSA) were prepared and characterized by transmission electron microscopy and thermogravimetry. The amount and distribution of colloidal silver per particle were related to the dissociation ratio of carboxyl groups in the PSA sphere. The amount of carboxyl groups was evaluated by a conductivity titration curve. However, the dissociation of carboxyl groups on PSA is difficult to determine accurately via existing methods because the dissociation ratio will increase with increasing impurity ions during titration. We developed a technique to determine the dissociation ratio of PSA without impurity ions. This employs a novel distance-variable parallel electrode system. Thus, the relationship between nano silver distribution and natural dissociation of carboxyl groups on the surface of the PSA spheres was investigated for the first time. Accurately measuring and controlling the dissociation facilitated the production of PSA spheres containing highly dispersed silver nanoparticles. The catalytic performance of as-prepared nAg@PSA catalysts was studied by reduction of 4-nitrophenol. By controlling the amount of natural dissociation ratio of carboxyl group on PSA sphere, dispersion of silver nanoparticles can be designed and attained controllably. They offer easy synthesis, high catalytic performance, and good recyclability.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Keqin Deng,Hao Wang,Jing Xiao,Chunxiang Li,Shaowei Zhang,Haowen Huang
A sandwich-type photoelectrochemical (PEC) immunosensor was constructed for sensitive detection of prostate specific antigen (PSA). It was based on electrochemically reduced graphene oxide-TiO 2 (ERGO-TiO 2 ) as photoelectrochemical platform to immobilize capture antibody (Ab 1 ). Then, quinone-rich polydopamine nanospheres (PDANS) loaded detection antibody (Ab 2 ) and photocurrent signal label, l -cysteine-coated cadmium sulfide quantum dots (CdSQDs). ERGO-TiO 2 displayed greatly improved photocurrent response to white light. CdSQDs conjugated with PDANS further amplified photocurrent signal because of the good conductivity of PDANS and ERGO. The increased photocurrent showed a linear correlation with PSA in the concentration range from 0.02 pg mL −1 to 200 ng mL −1 with the detection limit of 6.8 fg mL −1 . It also revealed high selectivity and good stability.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Chuanqing Zhao,Fangke Qiao,Jiawei Ji,Shifeng Deng,Huimin Qi
Polymer-reinforced silica aerogels are thermally insulating materials employed to enhance mechanical properties; however, they exhibit low heat stability and require a complex production process. The main body of this work concerns the synthesis of silicon-containing polyarylacetylene (PSA) resin, which has exceptional thermal properties and is used to strengthen the gel skeleton and significantly improve the heat resistance of the polymer reinforcement phase. The honeycomb-like porous SiO2/PSA aerogels derived from directional freezing were obtained via click reaction, gel aging, freeze-drying, and curing without the requirement for time-consuming solvent replacement. The prepared SiO2/PSA aerogel is low density (∼0.3 g/cm3) and high porosity (∼80%), which provides the material with low levels of thermal conductivity (∼0.06 W/m·K) and excellent thermal insulation performance. When compared to the majority of polymer aerogels and aerogel-like materials, the prepared SiO2/PSA aerogels have high Td5 (∼460 °C) and Yr800 (∼80%) and compressive strength (compression strength > 1.5 MPa). SiO2/PSA composite aerogel has numerous functions in areas where materials must withstand extremely elevated temperatures, such as the aerospace industry.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·
IF 2.7
Zhisong Li,Linbo Wu
Poly(ethylene 2,5-furandicarboxylate) (PEF) is an important biobased polyester with better thermal, mechanical, and gas barrier properties than poly(ethylene terephthalate). In PEF synthesis, diethylene glycol furandicarboxylate (DF) is inevitably formed via etherification side reaction. Taking advantage of the side reaction, poly(ethylene-co-diethylene furandicarboxylate) (PEDF) poly(ether-ester)s were synthesized only from 2,5-furandicarboxylic acid (FDCA) and ethylene glycol (EG), using a cheap binary propanedisulfonic acid/dibutyltin oxide (PSA/DBTO) catalyst. The polymers were characterized by 1 H NMR, intrinsic viscosity, solubility, differential scanning calorimetry, thermogravimetric analysis, tensile, and gas barrier test. Due to strong acidity of PSA, not only major DF but also minor oligoethylene glycol furandicarboxylate ether-containing units were formed. Their total content can be adjusted up to 78 mol% by simply increasing the dosage of PSA from 0 to 0.4 wt%. The effect of ether-containing units on the solubility, thermal transition, mechanical, and oxygen barrier properties of the poly(ether-ester)s were examined and compared with the poly(ether-ester)s synthesized via copolycondensation.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Current Molecular Pharmacology
·
2024
·Vol. 17
·期 1
·e18761429315125
·
IF 2.4
YiDan Li,BingHua Tu,ZiTong Wang,ZiChen Shao,ChenHao Fu,JianQiang Hua,ZiWen Zhang,Peng Zhang,Hui Sun,ChenYan Mao,Chi-Ming Liu
Background Finasteride and doxazosin are used for the treatment of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Epithelial–mesenchymal transition (EMT) and TGF-β/Smad signaling pathway play an important role in BPH, little is known about the growth inhibition and anti-fibrosis effects of doxazosin on the regulation of EMT and morphology in the prostate. Objectives The present study examined the effects of doxazosin on testosterone propionate (TP)-induced prostate growth in vivo and in vitro and its impact on the EMT and TGF-β/Smad signaling pathway. Methods Mice were treated with TP. Doxazosin (5 or 10 mg/kg) and finasteride (10 mg/kg) were administered orally for 28 days in TP-induced mice. The prostate index (prostate/body weight ratio), morphological characteristics and the protein expression of the prostate were examined. We further examined the effects of doxazosin and finasteride on the EMT and TGF-β/Smad signaling pathway in mice and in human prostate stroma cell (WPMY-1). The protein expressions of TGF-β1, TGFBR2, p-Smad2/3, N-cadherin, vimentin, fibronectin and α-SMA, E-cadherin and prostate specific antigen (PSA) were determined after treatment by western blot. Results The prostate wet weight, prostate index decreased after treatment. Doxazosin (5 or 10 mg/kg), finasteride (10 mg/kg) or a combination treatment (doxazosin 10 mg/kg + finasteride 10 mg/kg) were shown to reverse the pathological and morphological characteristics of the prostate. Doxazosin and finasteride inhibited TP-induced prostate growth, EMT, and the TGF-β/Smad signaling pathway by downregulating the expression of TGF-β1, TGFBR2, p-Smad2/3, N-cadherin, vimentin, fibronectin and α-SMA, whereas expression of E-cadherin was increased after treatment with either doxazosin or finasteride. Doxazosin (1-50 μM) inhibited normal human prostate stroma cell growth (WPMY-1) after 48 h with or without testosterone treatment. Doxazosin also regulated the EMT and proteins related to the TGF-β/Smad signaling pathway in WPMY-1 cells after 24 h. Additionally, doxazosin decreased protein expression of the PSA both in vivo and in vitro. Conclusion This study demonstrated that doxazosin inhibits prostate growth by regulating the EMT and TGF-β/Smad signaling pathways in the prostate. This finding suggests that doxazosin has potential as a new signaling pathway for the treatment of BPH.
研究领域:
医学
·
药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）