LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Le Wang,Mingwu Zang,Xiaoyu Cheng,Shouwei Wang,Xin Zhao,Bing Zhao,Dan Li
This study aimed to explore the effects of storage on lipid profiles changes and potential candidates of lipid oxidation in dried shrimps using chemical and lipidomics analysis. Results showed that peroxide value (POV) and thiobarbituric acid-reactive substances (TBARS) significantly increased. A total of 790 lipid molecular species belonging to 7 main classes were identified, among which glycerophospholipids (GPs, 72.91%) were the most abundant, followed by glycerolipids (GLs, 13.67%) and sphingolipids (SPs, 8.10%). Phosphatidylcholine (PC), phosphatidylethanolamine (PE), triglyceride (TG) and diglyceride (DG) as dominant lipid species were susceptible to oxidization/hydrolyzation mainly because of their unsaturated structures. Using multivariate statistical analysis, 163 differential lipid molecules were screened, including 131 and 32 differentially abundant lipids (DALs) accumulating at high and low abundance levels, respectively. After storage, most of phospholipids significantly decreased, while corresponding lysophospholipids increased. Particularly, it was found that TGs and DGs with fatty acids of 18:1 and 18:2 were more likely to be downregulated. Furthermore, 18 DALs were selected as potential biomarkers of lipid oxidation in dried shrimps. This study provided insights into lipid oxidation mechanism and the changes in lipid profiles of dried shrimps during storage.
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DOI
高影响因子
MICROBIAL PATHOGENESIS
·
2026
·
IF 3.5
Xin Yu,Zhengxu Chen,Yaobing Li,Songhui Xie,Tengwen Zhang,Luqing Pan
This study investigated the distinct regulatory effects of a novel complex feed additive (quercetin: cinnamaldehyde: glycerol monolaurate = 1:1:2) on lipid metabolism, immune response, and antiviral defense in Penaeus vannamei (initial weight of 0.40 ± 0.13 g). During a 42-day feeding trial, both 0.1 % and 0.2 % complex feed additive (CFA)-supplemented groups modulated hepatopancreatic lipid metabolism by co-regulating the constitutive co-activator of PPARγ (CCPG) and the PI3K-AKT and PTGS2 pathways, promoting lipolysis, suppressing lipid synthesis, and significantly reducing lipid accumulation in the body and plasma ( P < 0.05). CFA also activated the JAK-STAT pathway and specifically modulated the NF-κB and p38 MAPK-AP-1 pathways, significantly improving the hemocyte count (THC) and phagocytic activity (PA), and upregulating the expression of antimicrobial peptides and lysozyme, while suppressing inflammatory factors (TNFα, IL16) in both hemocytes and the intestine ( P < 0.05). Moreover, CFA improved gut microbiota composition, with key differential genera positively correlating with host immune indices. Under white spot syndrome virus (WSSV) challenge, CFA significantly inhibited viral replication and infection ( P < 0.05), thereby enhancing antiviral efficacy and improving shrimp survival, with the best results observed at the 0.2 % concentration. Collectively, this study demonstrates that dietary supplementation with 0.2 % CFA effectively regulates lipid metabolism and strengthens both innate and antiviral immunity in P. vannamei , providing mechanistic insights into host-pathogen interactions and the development of antibiotic alternatives for sustainable aquaculture.
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PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Le Zhang,Yihuan Chen,Yinghao Zhou,Chen Chen,Yaohuan Wang,Yiming Zheng,Shilin Shang,Maocang Yan,Xuan Dong,Qianjin Zhou,Jiong Chen
Honokiol (HKL) sensitizes Vibrio parahaemolyticus to doxycycline. • HKL–DOX shows additive interaction and reduces culturable bacteria. • HKL–DOX is associated with membrane perturbation and ATP depletion. • Dietary HKL–DOX improves shrimp survival and lowers bacterial burden. • Host and microbiota changes accompany HKL–DOX-mediated protection. Vibrio parahaemolyticus is a major bacterial pathogen in shrimp aquaculture, and the development of new anti-infective strategies is urgently needed due to limited protective options. In this study, we report that the plant-derived compound honokiol (HKL) acts as an effective adjuvant to doxycycline (DOX) against V. parahaemolyticus . Checkerboard assays revealed an additive interaction between HKL and DOX (FICI = 0.5625), accompanied by a 16-fold reduction in the minimum inhibitory concentration (MIC) of DOX from 64 μg/mL to 4 μg/mL. Plate-count-based time-kill analysis showed that the HKL–DOX combination reduced recoverable culturable bacteria to below the detection limit under the tested plating conditions. Mechanistic investigations indicated that HKL acted as an important membrane-active component of the combination, increasing bacterial membrane permeability and perturbing membrane integrity. Under combination conditions, HKL and DOX induced membrane-associated damage and intracellular ATP depletion. In a shrimp infection model, the HKL-DOX combination therapy significantly improved shrimp survival, reduced bacterial load in the hepatopancreas, partially preserved intestinal and hepatopancreatic histoarchitecture, was accompanied by changes in immune- and mucosal barrier-related gene expression and intestinal microbiota diversity and composition. Overall, HKL acts as an effective antibiotic adjuvant, highlighting a promising combination strategy for controlling V. parahaemolyticus infections in aquaculture.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Jing-jing Fu,Jie Deng,Bo-yang Tan,Yi-shu Ni,Xiu-ping Dong,Si-min Zhang,Yue-wen Chen
This study investigated the effects of combining natural deep eutectic solvents (NADES) with sous-vide cooking (SVC) on the freeze-thaw (F-T) stability of Penaeus vannamei . Four NADES were prepared using different molar ratios of proline (Pro), glucose (Glu), and sorbitol (Sor), named PG52, PG21, PG11, and PS11, respectively. Among them, PS11 was selected for combination with SVC (NSVC70) for PV pre-maturation prior to F-T cycling due to its lowest polarity and viscosity, as well as superior low-temperature stability. Compared to SVC treatment alone, NSVC70 significantly reduced cooking loss during pre-maturation and mitigated water migration in SVC-treated samples during F-T cycles. Additionally, NSVC70 improved muscle fiber density and water retention. This protective effect may be attributed to the inhibition of ice crystal growth and recrystallization by NADES. This study provides theoretical support for NADES as an effective antifreeze and water retaining agent to protect SVC processed muscle foods during frozen storage.
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PubMed
高影响因子
JOURNAL OF MICROBIOLOGY
·
2020
·Vol. 58
·期 8
·675-686
·
IF 2.845
Zou Peizhuo,Yang Qian,Wang Hailiang,Xie Guosi,Cao Zhi,Chen Xing,Gao Wen,Huang Jie
Acute hepatopancreatic necrosis disease (AHPND) is one of the most significant bacterial diseases in global shrimp culture, causing severe economic losses. In the present study, we carried out in vitro antimicrobial tests to investigate the disinfection efficacy of 14 common disinfectants toward different AHPND-causing Vibrio spp., including eight isolates of V. parahaemolyticus , four isolates of V. campbellii , and one isolate of V. owensii . Polyhexamethylene biguanidine hydrochloride (PHMB) was revealed to possess the strongest inhibitory activity. Through analyzing and evaluating the results of antimicrobial tests and acute toxicity test, we selected PHMB and hydrogen peroxide (H 2 O 2 ) for further clinical protection test. Clinical manifestations indicated that both PHMB (2 mg/L and 4 mg/L) and H 2 O 2 (12 mg/L) could effectively protect juvenile Penaeus vannamei from the infection of V. parahaemolyticus isolate Vp 362 at 10 6 CFU/ml, and the survival rate was over 80%. When the bacterial concentration was reduced to 10 5 CFU/ml, 10 4 CFU/ml, and 10 3 CFU/ml, the survival rate after treated by 1 mg/L PHMB was 64.44%, 93.33%, and 100%, respectively. According to the results, PHMB and H 2 O 2 showed a lower toxicity while a better protection activity, particularly against a lower concentration of the pathogens. Therefore, these two disinfectants are proved to be promising disinfectants that can be applied to prevent and control AHPND in shrimp culture. Moreover, the methods of this study also provided valuable information for the prevention of other important bacterial diseases and suggested a reliable means for screening potential drugs in aquaculture.
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PubMed
高影响因子
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