Frontiers in Microbiology
·
2020
·
IF 4.236
Zhang Lu-Lu,Huang Wen,Zhang Ying-Ying,Fan Gang,He Jin,Ren Jing-Nan,Li Zhi,Li Xiao,Pan Si-Yi
α-Terpineol has been widely used in daily chemical, pharmaceutical, food, and flavor industries due to its pleasant odor with high economic value and pharmacological action. Our previous study showed that Penicillium digitatum DSM 62840 was an efficient biocatalyst for the transformation of limonene to α-terpineol. Thus, it was meaningful to explore the genome features and the gene expression differences of strain DSM 62840 during limonene biotransformation, and the detailed bioconversion pathways. In this study, the functional genes related to limonene bioconversion were investigated using genome and transcriptome sequences analysis. The results showed that the P. digitatum DSM 62840 genome was estimated to be 29.09 Mb and it encoded 9,086 protein-encoding genes. The most annotated genes were associated to some protein metabolism and energy metabolism functions. When the threshold for differentially expressed genes (DEGs) was set at twofold ratio, a total of 4,128, and 4,148 DEGs were identified in P_L_12h (limonene-treated condition) compared with P_0h (blank) and P_12h (limonene-untreated blank), respectively. Among them, the expression levels of genes involved in the biosynthesis of secondary metabolites, energy metabolism and ATP-binding cassette (ABC) transporters were significantly altered during the biotransformation. And the reliability of these results was further confirmed by quantitative real-time polymerase chain reaction (RT-qPCR). Moreover, we found that the enzyme participated in limonene biotransformation was inducible. This enzyme was located in the microsome, and it was inhibited by cytochrome P450 inhibitors. This indicated that the cytochrome P450 may be responsible for the limonene bioconversion. Several differentially expressed cytochrome P450 genes were further identified, such as PDIDSM_85260 and PDIDSM_67430, which were significantly up-regulated with limonene treatment. These genes may be responsible for converting limonene to α-terpineol. Totally, the genomic and transcriptomic data could provide valuable information in the discovery of related-genes which was involved in limonene biotransformation, pathogenicity of fungi, and investigation of metabolites and biological pathways of strain DSM 62840.
研究领域:
微生物学
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基因组学
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分子生物学
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生物技术
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生物化学
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DOI
PubMed
高影响因子
RSC Advances
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2014
·Vol. 4
·期 49
·25983-25992
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IF 3.708
Jun Liu,Wenjing Wang,Huiwen Liu,Yaoliang Zhou,Haibo Zhang,Xiaohai Zhou
Herein, amino-epoxy supports were innovatively imported onto magnetic nanoparticles (Fe3O4–polymer hybrid nanospheres) for immobilizing enzymes. This new support has a coating layer with dual functional groups (epoxy and amino-epoxy). Consequently, this support has great anionic exchange power and a high number of epoxy groups. The acquired immobilized Penicillium expansum lipase in combination with this heterofunctional support represents a novel class of heterogeneous catalyst towards the synthesis of 1,3-dibutylurea from ethylene carbonate and butylamine, which has not been very commonly catalyzed by enzymes. After optimization of the reaction conditions, the yield of 1,3-dibutylurea was 77% under solvent free conditions at 60 °C. Moreover, after completion of reaction, the catalyst was simply recovered by an external conventional magnet and recycled without significant loss in the catalytic activity (up to ten cycles).
研究领域:
材料科学
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生物化学
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催化
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纳米技术
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2024
·
IF 5.6
Mei-yuan Li,Zhi-peng Jia,De-min Li,Man-ping Luo,Ying-ying Meng,Kang- xu Wang,Bei Wang,Bing Liu,Xin-guo Zhang,Qin Li
Glycyrrhizic acid (GA) and glabridin (GD) are very important sweeteners and whitening agents present in Glycyrrhiza glabra L. This study investigates the cellulase and pectinase produced by endophytes in G. glabra to optimize and enhance the extraction of GA and GD from licorice using enzyme-assisted extraction (EAE) and microbial co-culture-assisted extraction (MCAE) approaches. Twelve strains with cellulase and pectinase activities were isolated from G. glabra. Notably, the strain G1–5, preliminarily identified as Penicillium sp., demonstrated superior performance in enzyme production and extraction efficiency of GA. Compared to the traditional extraction methods, the EAE process showed an obvious improvement of 32.21% and 56.37% in GA and GD extraction rates, respectively. Similarly, the MCAE process with the enzyme-producing strain G1–5 exhibited an increase of 25.52% and 38.45% in GA and GD extraction rates, respectively. These findings provide valuable guidance and innovative strategies for the extraction of active ingredients.
研究领域:
植物化学
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生物技术
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药用植物学
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产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2022
·
IF 6.751
Yonghua Zhang,Qiuli OuYang,Bin Duan,Okwong Oketch Reymick,Yue Chen,Yuanzhen Tan,Xiangrong Zhu,Donglin Su,Gaoyang Li,Nengguo Tao
Green mold, caused by Penicillium digitatum , is the primary postharvest disease in citrus fruit. Trans -2-hexenal was found to exert a considerable inhibitory effect on the mycelial growth of P. digitatum with a minimum fungicidal concentration (MFC) of 0.106 g L −1 . To overcome its hydrophobic restriction, a trans -2-hexenal- β -cyclodextrin inclusion complex ( β -CDIC) was prepared by the saturated aqueous solution method. In vitro assays revealed that the MFC of β -CDIC for P. digitatum mycelial growth was 1.00 g L −1 , and 10.0 g L −1 β -CDIC apparently reduced the incidence of green mold in citrus fruit inoculated with P. digitatum while maintaining fruit quality. The structure of β -CDIC was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform-infrared spectroscopy (FT-IR), thermogravimetric (TG), and nuclear magnetic resonance (NMR) analyzes. The results showed that the H-1 proton of trans -2-hexenal correlated with the H-5′ proton of β -cyclodextrin ( β -CD), which was further confirmed by molecular docking analysis. The present study suggests that β -CDIC is a promising candidate for controlling postharvest diseases in citrus fruit.
研究领域:
植物病理学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2020
·
IF 1.992
Jinxin Che,Xiumei Chen,Qiuli Ouyang,Nengguo Tao
Penicillium digitatum and P. italicum are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, p -anisaldehyde exhibited a strong inhibitory effect on P. digitatum and P. italicum , with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.00 μl/ml. Additionally, p -anisaldehyde visibly inhibited both the green mold and blue mold development of citrus fruits inoculated with P. digitatum and P. italicum . The mycelia morphologies of these pathogens were greatly altered, and the membrane permeability and cell wall integrity of mycelia were severely disrupted under p -anisaldehyde treatment. These results suggest that the antifungal activity of p -anisaldehyde against P. digitatum and P. italicum can be attributed to the disruption of the cell wall integrity.
研究领域:
植物病理学
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食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2024
·
IF 5.3
Fu Jin-Xian,Jiao Jiao,Gai Qing-Yan,He Xiao-Jia,Fu Yu-Jie,Feng Xue,Gao Jie
Baicalein and wogonin, two health-promoting flavonoid aglycones in Scutellaria baicalensis Georgi roots, have better bioactivity and bioavailability than their glycoside precursors (baicalin and wogonoside). The search for novel microorganisms for the fermentation of S. baicalensis roots to improve the yields of baicalein and wogonin is of great interest in the food and nutraceutical industries. In this study, a novel endophytic fungus ( Penicillium rubens ) capable of producing cellulase (0.82 ± 0.12 U/mL) and β-glucosidase (4.55 ± 0.97 U/mL) was used to ferment S. baicalensis roots to obtain high yields of baicalein and wogonin. Semi-solid-state fermentation with homogeneous fungal cultures was found to be the appropriate strategy. Under the optimal fermentation parameters (temperature 30 °C, inoculation dose of fungal cultures 4 mL/g, and time 60 h), the yields of baicalein and wogonin (46.95 ± 4.98 mg/g DW and 31.41 ± 9.36 mg/g DW) from S. baicalensis roots were tremendously increased by 16.13-fold and 15.47-fold over control, respectively. Results of industrial enzyme bioprocessing and scanning electron microscope indicated that the remarkable increase in aglycone product yields could be due to the synergistic effects of cellulase and β-glucosidase produced by P. rubens on cell wall hydrolysis and glycoside deglycosylation. In addition, extracts of S. baicalensis roots fermented by P. rubens showed a significant increase in antioxidant and antimicrobial activities. Overall, the semi-solid-state fermentation of S. baicalensis roots using the novel P. rubens was an effective strategy that could yield high quantities of baicalein and wogonin, which had promising potential in the food and nutraceutical industries. Graphical Abstract
研究领域:
药用植物学
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微生物学
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食品科学
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天然产物化学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2019
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IF 6.669
Jian Du,Jingrui Liang,Xiuhua Gao,Guodong Liu,Yinbo Qu
Optimization of the composition of cellulase mixtures is an effective strategy to improve their hydrolytic efficiency and reduce protein demand during enzymatic degradation of lignocelluloses. In this study, the mixture design method was used to optimize the ratios of endoglucanase II (EG II), cellobiohydrolase I (CBH I) and β-glucosidase I (BG I) from Penicillium oxalicum in an artificial cellulase mixture for the hydrolysis of six different cellulosic materials. The optimal composition of enzyme mixture was distinctly different among not only cellulosic materials with different pretreatment methods but hydrolyses at different solids concentrations. CBH I was most critical for the hydrolysis of two acid-pretreated materials, probably due to its strong adsorption on lignin. A higher proportion of EG II was needed for the hydrolysis of ammonium sulfite pretreated wheat straw. The requirements of specific cellulase components were more pronounced at high solids concentrations, highlighting the importance of considering solids loading when optimizing cellulase cocktails.
研究领域:
生物技术
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酶学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
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2014
·Vol. 12
·期 4
·1815-1838
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IF 3.512
Chang-Jing Wu,Chang-Wei Li,Cheng-Bin Cui
AD-2-1 is an antitumor fungal mutant obtained by diethyl sulfate mutagenesis of a marine-derivedPenicillium purpurogenumG59. The G59 strain originally did not produce any metabolites with antitumor activities in MTT assays using K562 cells. Tracing newly produced metabolites under guidance of MTT assay and TLC analysis by direct comparison with control G59 extract, seven new (1–7) and two known (8–9) lipopeptides were isolated together with five known polyketides10–14from the extract of mutant AD-2-1. Structures of the seven new compounds including their absolute configurations were determined by spectroscopic and chemical evidences and named as penicimutalides A–G (1–7). Seven known compounds were identified as fellutamide B (8), fellutamide C (9), 1′-O-methylaverantin (10), averantin (11), averufin (12), nidurufin (13), and sterigmatocystin (14). In the MTT assay,1–14inhibited several human cancer cell lines to varying extents. All the bioassays and HPLC-photodiode array detector (PDAD)-UV and HPLC-electron spray ionization (ESI)-MS analyses demonstrated that the production of1–14in the mutant AD-2-1 was caused by the activated production of silent metabolites in the original G59 fungal strain. Present results provided additional examples for effectiveness of the chemical mutagenesis strategy using diethyl sulphate mutagenesis to discover new compounds by activating silent metabolites in fungal isolates.Keywords:marine-derived fungus;lipopeptide;penicimutalide;Marfey analysis;polyketide;Penicillium purpurogenum;DES mutagenesis
研究领域:
微生物学
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药物化学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2025
·
IF 2.7
Zixuan Lin,Tingting Hu,Lin Lin,Tao Li,Yan Yan,Wei Wei,Dongzhi Wei
Although α-amylases are critical biocatalysts in starch processing, fungal α-amylase diversity remains largely untapped. This study presents the first systematic characterization of an α-amylase from Penicillium brevicompactum NRRL864. Maximum α-amylase activity of 195 U/mL was obtained at pH 6.0 and 55°C under optimized fermentation conditions. These conditions consisted of 0.75% starch, 3% peptone, 0.5% NaCl, incubation at 20°C for 7 days. A novel α-amylase gene (DF) was identified via phylogenetic analysis and heterologously expressed in Escherichia coli . The gene encodes a 551-amino-acid α-amylase with an open reading frame of 1656 bp, showing 68.36% sequence identity to the α-amylase from Penicillium expansum . The DF enzyme displayed a specific activity of 489.4 U/mg under optimal conditions of 50°C and pH 6.0, and a 124.2% activity enhancement with 1 mM Ca 2 ⁺, indicating Ca 2 ⁺-dependence. The activity of DF was inhibited by a range of chemical reagents, including EDTA and urea. Substrate specificity assays confirmed that the DF enzyme exhibited optimal activity in hydrolyzing soluble starch. DF-mediated saccharification of corn starch yielded a reducing sugar concentration of 317.9 µg/mL within 70 min, confirmed by thin-layer chromatography. Beyond identifying P. brevicompactum as a novel α-amylase source, this work demonstrates DF as a Ca 2 ⁺-responsive GH13 enzyme with significant potential for industrial applications in biofuel and sweetener production.
研究领域:
生物技术
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酶学
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微生物学
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工业生物催化
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分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2016
·
IF 2.429
Jinwei Zhang,Zhangwei Han,Bo Teng,Wuyong Chen
For getting a clear and detail process of chromium tanned leather biodeteriorated with Penicillium sp. as a reference for future research and providing a cleaner chromium containing leather waste disposal method. The chromium tanned leather (CTL) and chromium tanned hide powder (CTHP) were biodeteriorated with the Penicillium . The CTL was biodeteriorated at first to verify the biodeterioration effect, and then the changes of the CTHP structure and thermal properties after the biodeterioration were analyzed by Scanning Electron Microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), Thermal Gravimetric Analyzer (TG) and Differential Scanning Calorimeter (DSC). The deterioration degree of the CTHP was characterized by the contents of hydroxyproline and total nitrogen in the filtrate, the Penicillium number was counted too during the process. The results showed that the CTL could be biodeteriorated with the Penicillium as shrinkage temperature and mass decreasing even though the biodeterioration degree was not ideal enough. After the process of CTHP biodeteriorated with the Penicillium , the surface morphology and structure of the CTHP were damaged. The thermal resistance and thermal denaturing temperature reduced which showed the detanning effect happened in the biodeterioration process. With the increasing of biodeterioration time, the hydroxyproline and total nitrogen in the filtrate increased. The Penicillium number maximized at 96 h and protease was produced in the process with enzymatic activity reaching to 9.23U mL −1 . These phenomena demonstrated the Penicillium grew through digesting chromium tanned leather and produced metabolites. To sum up, the biodeterioration process of chromium tanned leather with Penicillium was a synergistic effect of chromium detanning and the Penicillium growth.
研究领域:
微生物学
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环境科学
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皮革化学
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DOI
高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2022
·
IF 4.803
Jiaqi Wang,Taiwei Li,Pinmei Wang,Wanjing Ding,Jinzhong Xu
Twelve new tanzawaic acid derivatives, penitanzacids A−F (1–6), and G−J (9–12), and hatsusamides C−D (13–14), together with two revised structures [tanzawaic acids I−J (7–8)] and three known compounds (15–17) were isolated from the deep-sea-derived fungus Penicillium sp. KWF32. Their structures including absolute configurations were elucidated by spectroscopic data analysis, HRESIMS data, modified Mosher’s method, chemical degradation studies, ECD calculations, single crystal X-ray diffraction, and biogenic considerations in comparison with reported known analogues. Penitanzacids H–J (10–12) represent the first examples of this family with a C3 side chain and support the proposed biosynthetic pathway in which the side chain is connected to the decalin backbone. Hatsusamides C–D (13–14) have a hybrid skeleton formed by linking a tanzawaic acid and a diketopiperazine through an ester bond. Compounds 13 and 14 exhibit weak cytotoxicity against the A549 cell line.
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DOI
PubMed
高影响因子
Food Bioscience
·
2019
·
IF 3.22
Weilu Guo,Mouming Zhao,Qingjin Chen,Li Huang,Yan Mao,Ning Xia,Jianwen Teng,Baoyao Wei
The ability of a strain to produce citrinin was analysed in the growth inhibition zone using HPLC and UHPLC–MS. A total of 12 Penicillium citrinum strains isolated from Liupao tea were inoculated onto tea leaves and their capability for citrinin production were studied using different water content (15–45%) and temperatures (15–40 °C). The results provided a theoretical base for the safe manufacture of Liupao tea. Five strains of P. citrinum produced a clear growth inhibition zone with diameters of 11–22 mm. The citrinin contents in the liquid cultures of the 12 strains of P. citrinum ranged from 0.03 to 14.5 μg/mL. The citrinin contents of the teas with initial water content of 15 and 25% and those inoculated with P. citrinum were below the limit of detection during the fermentation. The citrinin content of the teas with initial water contents of 35 and 45% at 25 °C increased rapidly at first and then decreased during the fermentation. Similarly, the citrinin contents of the teas at 15 and 25 °C rapidly increased and then decreased. Meanwhile, P . citrinum was unable to produce citrinin at 35 and 40 °C. The optimum conditions were 45% water and 25 °C, generating the highest citrinin yield of 181 ng/g, which was higher than the limit of 50 ng/g citrinin in red yeast rice that can cause harm to human health. The highest citrinin yield in tea fermented for 50 days was 34.8 ng/g for stored teas with proper conditions. Therefore, storing teas with a low water content and temperature can reduce the content of citrinin.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）