Food Frontiers
·
2025
·
IF 6.9
Xiaole Zhao,Bing Zheng,Tianxiang Ao,Jianhua Xie,Yi Chen,Hesham El-Seedi,Hairong Huang,Baoting Liu,Qiang Yu
Polygonatum cyrtonema polysaccharide (PCP-80%) is one of the major active ingredients in Polygonatum cyrtonema . By establishing the type 2 diabetes mellitus (T2DM) rat model and applying multi-omics analysis, the present study aimed to explore the potential mechanism of PCP-80% to alleviate T2DM in terms of the interactions between intestinal microbiota and their metabolites. The results suggested that PCP-80% effectively improve the blood glucose and lipids levels, enhance intestinal barrier function. In addition, PCP-80% restored gut microbiota disorders, increased the secretion of short-chain fatty acids (SCFAs), and enhanced bile acid (BA)-converting enzyme-rich microbiota abundance. Furthermore, PCP-80% intervention significantly ameliorated BA dysmetabolism while enhancing metabolic homeostasis in T2DM rats. These changes activated the G protein-coupled bile acid receptor 1 (TGR5) and farnesoid X receptor (FXR), triggering fibroblast growth factor 15 (FGF15) secretion to ameliorate T2DM-induced intestinal barrier dysfunction and glucose/lipid dysregulation. Mechanistically, PCP-80% exerted therapeutic effects through a microbiota-bile acid crosstalk axis, wherein gut microbiota remodeling enhanced bile acid metabolism and amplified TGR5/FXR-FGF15 signaling, ultimately restoring metabolic homeostasis in T2DM rats.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qiang-Ming Li,Huan Xu,Xue-Qiang Zha,Feng-Yun Zhang,Jian-Ping Luo
Parkinson's disease (PD) has become a global health threat as a progressive neurodegenerative disorder. Polygonatum cyrtonema polysaccharide (PCP), the main bioactive constituent in P. cyrtonema rhizome, displays various biological activities. However, its antiparkinsonian effect is elusive. Here, the effect of PCP on PD-like pathology was evaluated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model and its molecular mechanism was further investigated. The results showed that PCP at different dosages significantly improved MPTP-induced weight loss and dyskinesia in PD mice. Moreover, PCP treatment mitigated the dopaminergic neuron loss in the substantia nigra pars compacta (SNc). Analysis of apoptosis-related proteins revealed that PCP markedly decreased the expression levels of Bax and cleaved-caspase 3, and increased the expression level of Bcl-2 in PD mice. Meanwhile, PCP markedly enhanced the activities of antioxidant enzymes superoxidase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), reduced malondialdehyde (MDA) content, and reversed the expression of oxidant stress-related proteins Nrf2 and Keap1, in the SNc of PD mice. Furthermore, PCP treatment significantly reduced the MPTP-mediated increase of endoplasmic reticulum (ER) stress-related proteins. These findings suggest that PCP protects against PD-like pathology by mitigating oxidant stress and ER stress-mediated apoptosis of dopaminergic neurons, highlighting its potential as a therapeutic agent for PD.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 7
·2200823
·
IF 3.1
Gaodan Liu,Simin Feng,Minghui Sui,Bilian Chen,Peilong Sun
In this study, quartz sand with particularly sharp nanoscale edges acted like a nanoscale knife physically cut cells of Polygonatum cyrtonema Hua into nanosized particles and was synergized with natural deep eutectic solvent to extract steroidal saponins of Polygonatum cyrtonema Hua. The natural deep eutectic solvent (choline chloride-lactic acid)-synergistic quartz sand-assisted extraction was optimized using response surface methodology. The steroidal saponins purified with AB-8 macroporous resin were identified using ultra-high-performance liquid chromatography-triple time of flight mass spectrometry. The results showed that the experimental total saponins content value (36.97 ± 0.12 mg dioscin equivalent/g dry weight) at optimal extraction conditions with a temperature of 68°C, a rotational speed of 20 400 rpm, shear time of 4.3 min, the liquid-solid ratio of 38 ml/g, was close to the maximum possible theoretical value (36.64 mg dioscin equivalent/g dry weight). A total of 20 steroidal saponins were identified, among which the content of (25 R )-Kingianoside E was the highest (102.66 ± 3.47 mg/g). Furthermore, a new steroid saponin (3 β ,25 S )-26-( β -D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl 4- O - β -D-glucopyranosyl- β -D-galactopyranoside+Glc was found for the first time. These results revealed that natural deep eutectic solvent-synergistic quartz sand-assisted extraction was an efficient and green method to extract a variety of steroidal saponins.
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Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-22
·
IF 5.2
Xia Lunbin,Chen Cunwu,Bi Shaoshuai,Zhang Yafei,Chen Naidong,Feng Jing
Background In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. Polygonatum cyrtonema Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated. Methods Crude Polygonatum cyrtonema Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments. Results Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 10 4 Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conformation, and SEM analysis revealed its smooth, porous, clastic or lamellar microstructure. Comprehensive FT-IR, GC–MS, and 1D/2D NMR analyses indicated that the main chain of PCHP-1A consists of → 1)-β- d -Fru f -(2 → , → 1,6)-β- d -Fru f -(2 → , and → 6)-α- d -Glc p, with branch chains primarily composed of β- d -Fru f -(2 → linked at the O -6 position of → 1,6)-β- d -Fru f -(2 →. Biological activity assays demonstrated that at a concentration of 4.0 mg/mL, PCHP-1A exhibited a DPPH scavenging rate of 56.92 ± 1.42% and an inhibitory capacity against · OH radicals of 23.27 ± 1.80 U/mL. Furthermore, PCHP-1A showed significant hypoglycemic activity, with an IC 50 value of 5.15 ± 1.09 mg/mL. Conclusions This study successfully isolated and purified polysaccharide from Polygonatum cyrtonema Hua, elucidated its fundamental structure, and investigated its antioxidant and hypoglycemic activities. The findings provide a theoretical foundation for understanding the structure–activity relationship of the polysaccharide and suggest that Polygonatum cyrtonema Hua polysaccharide may be a natural potential antioxidant and hypoglycemic agent. Graphical
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 2.6
Rong Ai,Fatima-Ezzahra Ettoumi,Yueran Ma,Dong Li,Weide Lv,Zisheng Luo
Summary The integration of carbon dioxide-nanobubbles (CO 2 -NBs) with cellulase has led to the development of an innovative extraction system for phenolic compound retrieval from P. cyrtonema (Polygonatum cyrtonema) Hua rhizomes. Four critical factors influencing on CO 2 -NBs-cellulase were methodically investigated and optimised through single-factor experiments. The optimal experimental conditions are 30 cycles, 0.35 MPa, pH 5.45 and 0.025 g/mL cellulase aqueous solution. The stability of system was characterised meticulously, indicating the viability for at least 24 h. Comparative assessment of bioactive constituents and antioxidant activity demonstrated exceptional extraction efficiency and potential synergistic effects of CO 2 -NBs-cellulase. Synergistic effects and reaction mechanisms during extraction were explored using Fourier-transform infrared spectroscopy, differential scanning calorimetry and scanning electron microscopy, which contributed to mechanism understanding with significant alterations in partial structure and heightened thermal stability we found. In essence, this study introduces an eco-friendly, energy-efficient extraction system paradigm based on a novel nanobubble-enzyme binding approach.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Hao Wang,Yuting Bao,Jinpeng Hu,Wenjia Yan,Shanshan Xu,Jiaying Huo,Jian Jiang,Zhuochen Wang
The traditional simultaneous distillation-extraction (SDE) method was innovatively employed to co -extract both volatile aroma compounds and water-soluble polysaccharides from steamed Polygonatum cyrtonema Hua (SPCH). Key aroma-active compounds were identified through molecular sensory science, revealing six components with OAV > 1, among which 2-acetyl-1-pyrroline and furfural were particularly crucial to the distinctive aroma profile. Through sequential ethanol precipitation, twelve polysaccharide fractions were obtained. Among them, a glycoprotein (95-C2) was selected for further investigation due to its relatively uniform molecular weight (Mw: 8.5 kDa; Mw/Mn = 1.5) and exceptional free radical scavenging activity (IC₅₀ = 0.1050 ± 0.0445 mg/mL for DPPH; 0.2323 ± 0.0264 mg/mL for ABTS). Consequently, 95-C2 was evaluated in a D-gal-induced senescence model using PC12 cells, where it significantly reduced SA- β -gal-positive staining, ameliorated cell cycle arrest and apoptosis, and suppressed intracellular ROS generation while restoring mitochondrial membrane potential. The senescence-associated secretory phenotype was mitigated through reduced secretion of TNF- α , IL-1β, and IL-6. Mechanistic studies at both gene and protein levels indicated that these anti-aging effects were potentially mediated through suppression of the p53-p21-p16 signaling pathway. This work represents the first attempt at simultaneous flavor-polysaccharide analysis using SDE, discusses their potential relationships, provides a referable framework for future research, and offers new insights into SPCH's potential in functional food applications.
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Foods
·
2026
·Vol. 15
·期 17
·2954
·
IF 6
Rongguang Yang,Luning Zhao,Ying Zhu,Yansheng Zhao,Juan Bai,Xiang Xiao
Polygonatum cyrtonemaHua polysaccharides (PCPs) are bioactive components with antioxidant and immunomodulatory properties. However, whether the polysaccharides derived from elderPolygonatum cyrtonemaexhibit superior health benefits remains unclear. This study systematically compared the in vitro fermentation characteristics, gut microbiota-modulating effects, and short-chain fatty acid (SCFA) production of PCPs extracted from three-year-old (TPP), five-year-old (FPP), and eight-year-old (EPP) plants using a human fecal fermentation model. The fermentation dynamics were evaluated by monitoring pH, OD600, and the consumption of total and reducing sugars, while the structural degradation and compositional differences in PCPs were tracked via molecular weight distribution and monosaccharide composition analysis. The results showed that all three PCPs were degraded and utilized by gut microbiota, accompanied by decreased pH, increased OD600, and enhanced antioxidant activities. High-throughput 16S rDNA sequencing revealed that, at the phylum level, all PCPs increased the Firmicutes/Bacteroidetes ratio. At the genus level, they reduced the abundance of harmful bacteria such asSutterellaand increased beneficial bacteria includingBifidobacteriumandMegasphaera. Furthermore, gas chromatography (GC) analysis demonstrated that, compared with FPP, TPP and EPP significantly promoted the production of SCFAs. In summary, this study indicates that the in vitro fermentation characteristics and prebiotic properties of PCPs vary with growth years, and a comprehensive evaluation suggests that three-year-oldPolygonatum cyrtonemaHua represents a promising raw material for the development of functional foods.
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Food Production Processing and Nutrition
·
2025
·Vol. 7
·期 1
·1-15
·
IF 5.7
Cao Tianzhuo,Hai Xiaoya,Zhao Chenchen,Wang Mengjin,Tong Zihan,Cui Hao,Zhou An,Liu Juan,Yu Nianjun,Xing Lihua
Polygonatum cyrtonema Hua ( P. Hua) has been demonstrated with several biological potentials, such as lipid lowering and anti-inflammatory effects. To evaluate protective the effect of P. Hua aqueous extract (PCHE) on metabolic-associated fatty liver disease (MAFLD), PCHE was analyzed by UPLC-Q-TOF MS to characterize the chemical composition and pharmacodynamic experiments in vivo and in vitro were conducted using PCHE to intervene in MAFLD models. In vitro, a MAFLD cellular model was established by inducing HepG2 cells with free fatty acid (FFA). In vivo, a MAFLD murine model was generated via a high-fat/high-fructose (HFHF) diet in C57BL/6 J mice. The results showed that a total of 32 chemical compounds were detected in PCHE. In both HepG2 cells and HFHF-induced MAFLD mice, high-dose PCHE significantly improved lipid profiles, inhibited excessive hepatic lipid droplet accumulation, and up regulated the mRNA and protein expression of PPARα/CPT-1A/MCAD. In conclusion, PCHE exerted regulatory effects on MAFLD, possibly associated with the modulation of the PPARα/CPT-1A/MCAD signaling pathway. These results provided an vital basis for the development of functional food made up of P. Hua to prevent MAFLD. Graphical
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Foods
·
2025
·Vol. 14
·期 19
·3396
·
IF 5.1
Shoucheng Pu,Jufang Gong,Meihao Sun,Zunhong Hu,Zhihua Wu
Polygonatum cyrtonema Hua, a plant with a long history of consumption in China, serves both medicinal and edible purposes, and it exhibits numerous pharmacological effects, including promoting kidney health and enhancing immune function. However, the effect and molecular mechanism of Polygonatum cyrtonema polysaccharides (PCPs) on hyperuricemia have not yet been reported. The hyperuricemic mice model was induced by the intraperitoneal injection of potassium oxonate (PO, 300 mg/kg), combined with the intragastric administration of hypoxanthine (HX, 300 mg/kg). Biochemical assays in mice revealed that PCPs markedly lowered high serum uric acid levels, suppressed xanthine oxidase (XOD) activity, and reduced the expression of inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Western blot analysis demonstrated that PCPs downregulated urate transporter 1 (URAT1), while H&E staining showed that PCPs effectively restored renal histological integrity. Here, we isolated and identified the PCPs, which consist mainly of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose, with a molar mass ratio of 0.5:2.15:0.47:16.58:3.66:1.09. Furthermore, the galactose residue that docked with both XOD and URAT1 molecules forms more hydrogen bonds and exhibits a lower binding energy, which enables the improved regulation of both targets. We have demonstrated for the first time the improving effect of PCPs on hyperuricemia, and revealed their regulatory mechanisms by modulating xanthine oxidase, inflammatory factors, and uric acid transporters. This study not only provides new insights into the anti-hyperuricemic activity of PCPs in mice, but also lays a foundation for its potential application in the functional foods of anti-hyperuricemia.
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JOURNAL OF AOAC INTERNATIONAL
·
2026
·
IF 1.7
Fan Xuanxuan,Hu Xian,Wang Weiting,Chen Naifu,Chen Naidong,Hao Jingwen,Yan Le,Xiao Jun,Zhong Yu,Zhang Manman,Xie Zhengjun
BackgroundPolygonatum cyrtonema Hua (PCH) is widely used in traditional Chinese medicine. The components and activity are important for simultaneous quality evaluation in PCH.ObjectiveThis study aimed to establish an accurate, rapid, and comprehensive quality evaluation method by near-infrared spectroscopy (NIR) for forecasting the total polysaccharides content (TPsC), total flavonoids content (TFC), and antioxidant activity (AA) in PCH.MethodsPCH samples were used to establish a model of TPsC, TFC, and AA in PCH by NIR combined with partial least-squares regression (PLS). To enhance the accuracy of the models and remove non-essential variables, we used multiple spectral preprocessing methods and multiple chemometrics to analyze the processed full spectrum, such as competitive adaptive reweighted sampling partial least-squares regression (CARS-PLS), moving-window partial least-squares regression (MW-PLS), and interval random frog partial least squares regression (iRF-PLS). The remaining samples were used to complete external validation.ResultsSatisfactory prediction results of PLS models combined with chemometrics were obtained. The optimal chemometric of TPsC, TFC, and AA selected 157, 105, and 134 variables, respectively. For the TPsC, TFC, and AA models, which incorporated optimal spectral preprocessing and chemometrics, the root-mean-square error of calibration (RMSEC, %) values were 0.743, 0.069, and 0.136, while the R2 values were above 0.95. The root-mean-square error of prediction (RMSEP, %) for these models were 1.00, 0.074, and 0.153, while R2 values were above 0.90. The remaining 20 samples were used to complete external validation, which confirmed the preeminent comparability and ability of the proposed method.ConclusionNIR combined with chemometrics provides an effective, fast, and nondestructive approach for evaluating quality via multiple indicators of PCH and provides a meaningful reference for evaluation of herbal medicine quality.HighlightsNIR combined with chemometrics offers an effective, quick, nondestructive way to evaluate herbal medicine quality with multiple indicators.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 5
·2186
·
IF 4.6
Hui Liu,Yuanyuan Zhao,Leijing Chen,Jiao Du,Hongyan Guo,Bin Wang
Traditional Chinese medicine (TCM) safety and effectiveness can be ensured by establishing a suitable quality assessment system. This work aims to develop a pre-column derivatization HPLC method forPolygonatum cyrtonemaHua. quality control. In this study, 1-(4′-cyanophenyl)-3-methyl-5-pyrazolone (CPMP) was synthesized and reacted with monosaccharides derived from P. cyrtonema polysaccharides (PCPs), followed by HPLC separation. According to the Lambert–Beer law, CPMP has the highest molar extinction coefficient of all synthetic chemosensors. A satisfactory separation effect was obtained under a detection wavelength of 278 nm using a carbon-8 column and gradient elution over 14 min, with a flow rate of 1 mL per minute. Glucose (Glc), galactose (Gal), and mannose (Man) make up the majority of the monosaccharide components in PCPs, and their molar ratios are 1.73:0.58:1. The confirmed HPLC method has outstanding precision and accuracy, establishing a quality control method for PCPs. Additionally, the CPMP showed a visual improvement from colorless to orange after the detection of reducing sugars, allowing for further visual analysis.Keywords:quality control;chemosensor;pre-column derivatization;P. cyrtonemaHua. polysaccharides
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Meixia Zheng,Hailan Su,Rongfeng Xiao,Jianfu Chen,Hong Chen,Kok Bing Tan,Yujing Zhu
A novel film composed of Polygonatum cyrtonema extracts (PCE), xanthan gum (XG), flaxseed gum (FG) and carboxymethyl cellulose (CMC) was prepared (XFCP). Addition of PCE has decreased the light transmittance, while enhanced the UV blocking performance, antioxidant activity, tensile strength and elongation at break of XFCP due to polysaccharides, polyphenols, and flavonoid in PCE. Structural analyses by FTIR and XRD indicated the hydrogen-bonding interaction between PCE, XG , FG and CMC . It was found that compared with the control sample, XFCP2.5% with the lowest WVTR was able to prolong the shelf life of mango. The overall quality of mango was also improved in terms of lower decay rate, weight loss rate, total soluble solid, and polyphenol oxidase, higher titratable acidity, V c , and superoxide dismutase than control mango upon 8 days of storage. This effectively expanded the application of PCE into food packaging in addition to merely as Chinese traditional medicine herbs.
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高影响因子
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