Analytical Methods
·
2020
·Vol. 12
·期 27
·3484-3489
·
IF 2.596
Xinyue Rao,Huan Jiang,Ling Li,Feng Xiang,Zhongde Liu
Any signals, if their intensities have a simple functional relationship with analyte concentration, can be applied for analytical purposes. Among them, pressure measurement as a signalling technique has been extensively utilized to develop portable and quantitative bioanalysis, with the advantages of highly sensitive detection of a variety of biomedical targets. In this contribution, it was found that polymyxin B sulphate (PMB) could significantly inhibit the catalytic ability of platinum nanoparticles (PtNPs) prepared in different protocols in the H2O2 breakdown reaction. By employing sodium citrate as the reducing agent to prepare the platinum nanoparticles (C-PtNPs) as an example and monitoring the pressure signal changes of the C-PtNP catalyzed H2O2 breakdown against the reaction time, a sensitive, cost-effective, rapid and reliable analysis method for PMB is established on the basis of the pressure signal readout. The proposed method has a detection limit of 28.6 nM and can selectively detect PMB in both POLY-MxB powder injection and human urine samples, demonstrating its potential in bioanalysis, which would be significant to address biological, clinical and medicinal requirements.
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2026
·Vol. 16
·期 33
·30145-30157
·
IF 6.1
Mingchen Zhang,Wei Zhao,Yang Zhou,Ruiting Shen,Yiqiong Sun,Lei Jiang,Jiantao Zhang
Diabetic wounds are a serious and increasingly prevalent complication in patients with diabetes, characterized by impaired skin regeneration. A major challenge for effective healing is recurrent bacterial infection, which often resists conventional treatment strategies. In this study, we grafted polymyxin B onto hyaluronic acid and employed Fe3+–EDTA as a cross-linking agent to fabricate HA-B@Fe hydrogels via ionic interactions. Based on the reversible and dynamic coordination between Fe3+ ions and the carboxyl groups of the hyaluronic acid, the resulting hydrogels exhibited shear-thinning behavior and excellent injectability. Based on the combined effects of polymyxin B and Fe3+, the HA-B@Fe hydrogel exhibited potent antimicrobial activity against a broad spectrum of bacteria, achieving >99% bactericidal rates against Escherichia coli (E. coli) and >94% against Staphylococcus aureus (S. aureus). In addition, the HA-B@Fe hydrogel demonstrated great biocompatibility, and showed significant therapeutic efficacy in a diabetic mouse wound model. The mice treated with HA-B@Fe showed a marked reduction in wound size (down to 19%), compared to a residual wound size of 32% in group treated with commercial dressings at day 7. Histopathological analysis further confirmed that HA-B@Fe treatment promoted epidermal regeneration and collagen fiber deposition, suggesting its potential as a promiseing strategy for the clinical treatment of diabetic wounds.
✓ 已验证
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2021
·Vol. 84
·期 11
·1057-1064
·
IF 2.044
Zhang Hanzhi,Li Feng,Dun Junling,Sun Ning,Liu Hao,Chen Guiliang
An analysis strategy that can simultaneously determine the amino acid site information and configuration for polymyxin (PM) components is reported in this study. The main components of PMB1, PMB2, and PMB1-I (the Leucine at position 7 changed to be Isoleucine) were purified from the mixture of PMB. The PMB component was hydrolyzed by hydrochloric acid to be a mixture of amino acids, which were derivatized with Marfey’s reagent of N α -(5-fluoro-2,4-dinitrophenyl)- l -alanine amide ( l -FDAA), and then, the configurations of amino acids were easily determined by HPLC–MS. However, the aforementioned method could not offer the information of amino acid at each site, a specific enzymatic hydrolysis method combined Edman degradation was developed. The fatty acyl-diaminobutyric acid (position 1) of PMB component was removed by ficin, and the PMB components were transformed to be PMB nonapeptide. The amino acids at each site of the nonapeptide were determined by N-terminal sequencing. Combining the results of the above methods, the amino acid information and configuration of each site in PM components can be determined, especially the l -Leucine and l -Isoleucine in PMB1 and PMB1-I. The workflow was useful for other PM analogues to identify the sequence and configuration for quality control.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Jun Wu,Tianshu Zhai,Jing Sun,Qingsong Yu,Yicheng Feng,Runwei Li,Hao Wang,Qiuhong Ouyang,Tiantian Yang,Qingyuan Zhan,Li Deng,Meng Qin,Fang Wang
The aim of this study was to improve the bioavailability of polymyxin B (PMB) in pulmonary nebulized drug delivery. To this end, we developed a nano-delivery system that penetrates the mucus barrier of the lung. Hydrophilic hyaluronic acid (HA) was combined with a water-in-oil system containing a poly (lactic acid)-glycolic acid copolymer of PMB to prepare [email protected] nanoparticles (NPs) with good surface properties. [email protected] NPs with suitable electrical properties, particle size, and good hydrophilicity prevented strong interactions between the NPs and mucus, thereby allowing more drugs to enter deeper into the lung. Compared to the free drug PMB, NPs had more than 2-fold higher mucus penetration efficiency in vitro and better delivery to infected alveolar cells during in vivo nebulization. NPs had better biocompatibility, which further reduced the drug toxicity. More importantly, NPs showed better antimicrobial therapeutic efficacy in the treatment of lung infections in mice. These findings may provide support for the clinical application of nebulized pulmonary antibiotics.
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
·
2026
·
IF 4.9
Han Ying,Yang Xinyao,Jia Yuhao,Liu Mingying,Sun Xinglin,Yu Dan,Zhang Dayi
Salmonella is a Gram-negative bacterium causing intestinal infections. Polymyxin B (PMB) is an effective antibiotic against Salmonella , but the emergence of antibiotic resistance poses a growing concern. To address this challenge, this study synthesized a new nanocarrier by functionalizing nano-montmorillonite (NMMT) with PMB by adsorption, namely NMMT@PMB. PMB adsorption on NMMT followed the Langmuir isotherm and pseudo‑first‑order kinetics, and its release kinetics followed a diffusion-controlled mechanism. Although the minimum inhibitory concentration (MIC) of NMMT@PMB (2.78 and 44.54 µg/mL) was slightly higher than that of free PMB (2 and 32 µg/mL) for PMB-sensitive (S AT ) and resistant (S AT−P ) Salmonella typhimurium , the nanocarrier demonstrated sustained long‑term antibacterial activity over time. The antibacterial mechanism was the disruption of bacterial membrane integrity. Additionally, ex vivo results demonstrated that NMMT@PMB regulated the microbial community composition, increasing the relative abundance of probiotic bacteria (from 72.34% to 75.20%) and inhibiting pathogenic bacteria (from 8.54% to 2.36%), thereby facilitating its return toward the original state. These findings suggest that NMMT@PMB is a promising nano-delivery system that may support sustained antibacterial action and microbiota homeostasis, offering a potential strategy for improved management of Salmonella infections.
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Juan Liu,Pingxiao Wu,Fang Wang,Wenchao Niu,Zubair Ahmed,Meiqing Chen,Guining Lu,Zhi Dang
The reuse of polymyxin B (PMB) has attracted extensive attention. Although the resistance mechanism to PMB is clear, there are few reports on the regulation mechanisms and effects of clay minerals on bacteria induced by PMB. The focus of this study is to investigate the multidrug resistance, cell morphology and physiological modification of Escherichia coli ( E. coli ) exposed to PMB in the presence and absence of clay minerals. To be specific, E. coli was cultured serially for 15 days in the increasing concentration of PMB, with or without halloysite or kaolinite . The potential influence mechanisms of halloysite and kaolinite on E. coli was analyzed by proteomics , antibiotic resistance testing, confocal laser scanning microscopy, scanning electron microscopy and Fourier transform infrared. The results showed that kaolinite could obviously promote the growth of bacteria. Moreover, compared with halloysite, kaolinite could stimulate the overexpression of PMB resistance-related proteins ArnA, ArnB and EptA in E. coli exposed to PMB, and promote the synthesis of peptidoglycan and activate glycolysis pathway to produce energy. In contrast, halloysite was able to regulate the production of low molecular weight thiols by E. coli to prevent bacteria from producing excessive reactive oxygen species , activate the oxidative phosphorylation pathway to supply energy for bacterial life activities, and reduce multidrug resistance of E. coli in a variety of ways. These findings are essential for exploring the impacts of clay minerals on the emergence and spread of multi-drug resistant strains in the environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Bingqing Zhang,Xueyong Li,Yiying Chen,Bo Chen,Yu Cheng,Hailing Lin,Wancai Que,Maobai Liu,Lili Zhou,Hui Zhang,Hongqiang Qiu,Chaoyang Wu
Polymyxin B (PB) is currently one of the last resort treatment options against carbapenem-resistant gram-negative bacterial pathogens. Pharmacokinetics/pharmacodynamics (PK/PD) guided therapeutic drug monitoring (TDM) of antibiotics is critical for optimizing dosage regimens to maximize efficacy, minimize toxicity, and delay the emergence of resistance. Currently, methods for determining PB in human plasma and epithelial lining fluid (ELF) are limited. In this study, we developed and validated a simple method for PB determination in human plasma and ELF using LC-MS/MS. Protein precipitation of the sample was conducted with 0.1% formic acid-acetonitrile. Polymyxin B1 and B2 were separated on a C 18 column and detected within 4 min by the mass spectrometer in the positive mode coupled with multiple reaction monitoring. The calibration curve range was 0.156–10.0 and 0.0156–1.00 μg/mL in the plasma for polymyxin B1 and B2, respectively, and was 0.0625–2.00 and 0.00625–0.200 μg/mL for polymyxin B1 and B2, respectively in bronchoalveolar lavage fluid. The accuracy of the intra- and inter-assay studies ranged from 80.6% to 114.9%, and the coefficients of variation for intra- and inter-day assays were less than 14.8%. Among a considerable number of patients, the average steady-state plasma concentration of PB was suboptimal. Moreover, the exposure to PB in patients with acute kidney injury (AKI) was considerably higher than that in patients without AKI. Meanwhile, a higher concentration of PB in ELF could be achieved than that in plasma after PB nebulization treatment. The established method was proven to be rapid, simple, and suitable for TDM of PB and PK/PD studies in human plasma and ELF.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Linlin Wang,Shaopu Liu,Wanjun Liang,Dan Li,Jidong Yang,Youqiu He
A fluorescent “turn off–on” pattern for the detection of herring sperm DNA (hsDNA) had been designed through utilizing the interaction between polymyxin B sulfate (PMBS) and hsDNA as an inherent performance and the fluorescent transformation of glutathione (GSH)-capped CdTe quantum dots (QDs) as an external manifestation. Due to the occurrence of the photoinduced electron transfer from the QDs to PMBS, the fluorescence of GSH-capped CdTe QDs could be effectively quenched by PMBS, causing the system into “off” state. With the addition of hsDNA, the quenched fluorescence of GSH-capped CdTe QDs could be restored for the reason that PMBS embedded into hsDNA double helix structure to form new complex and peeled off from the surface of GSH-capped CdTe QDs, leading the system into “on” condition. Corresponding experimental results illustrated that the relative recovered fluorescence intensity of GSH-capped CdTe QDs–PMBS system was near proportional to the concentration of hsDNA within the range of 0.059–15.0 μg mL −1 . This proposed method demonstrated a good linear correlation coefficient of 0.9937 and a detection limit (3 σ / K ) of 0.018 μg mL −1 for hsDNA. This dual-directional fluorescent biosensor overcame the selectivity problem commonly existed in the traditional mono-directional fluorescence detection mode and owned perfect analysis applications in biochemical DNA monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Junjie Chen,Weibin Fan,Jing Fan,Jiao Xie,Yan Wang,Yinhui Wang,Nengming Lin,Bin Lin
The clinical application of polymyxin B (PMB) is limited by its nephrotoxic effects, making the reduction of PMB-induced nephrotoxicity has become a pressing concern for clinicians. Tetrahydrocurcumin (THC), known for its beneficial characteristics in biological functions, presents an attractive option for intervention therapy to mitigate PMB-induced nephrotoxicity. However, the underlying mechanism of how THC mitigates PMB-induced nephrotoxicity is still poorly understood. Here, we first evaluated the potential of THC intervention therapy to mitigate PMB-induced nephrotoxicity in an in vitro model of PMB-induced cell injury. Moreover, we demonstrated that THC effectively protected HK-2 cells from PMB-induced apoptosis by using cell counting kit-8 and flow cytometry assay. THC could also suppress PMB-induced endoplasmic reticulum (ER) stress via PERK/eIF2α/ATF4/CHOP pathway. In addition, using PERK inhibitor GSK2606414 to inhibit ER stress also alleviated PMB-induced apoptosis. Taken together, these findings provide novel insights that THC possesses the ability to alleviate PMB-induced nephrotoxicity by inhibiting the ER stress-mediated PERK/eIF2α/ATF4/CHOP axis, which sheds light on the benefits of THC as an intervention strategy to reduce PMB-induced nephrotoxicity, thus providing a potential avenue for improved clinical outcomes in patients receiving PMB treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Yizhong Shen,Shaopu Liu,Ling Kong,Dan Li,Youqiu He
Thioglycolic acid-capped (TGA-capped) CdTe/CdS quantum dots (QDs) were prepared in the water phase. The interaction of TGA-CdTe/CdS QDs with polymyxin B sulfate (POBS) was investigated by ultraviolet–visible (UV–vis) absorption, fluorescence (FL), resonance Rayleigh scattering (RRS) spectroscopy and the measurement of fluorescence lifetime . Under optimum conditions, not only the UV–vis absorption and fluorescence spectra were changed, but also the RRS intensity was greatly enhanced. These phenomena offered useful techniques for the determination of POBS with fluorescence quenching and RRS methods . The linear ranges and detection limits of POBS were 0.09–5.25 μg mL −1 and 27.5 ng mL −1 for the fluorescence quenching technique, 0.02–6.0 μg mL −1 and 6.36 ng mL −1 for the RRS technique. Among them, the RRS approach obtained the higher sensitivity. Accordingly, a novel rapid, convenient, and highly sensitive RRS method for the determination of POBS was proposed and applied to detect POBS in pharmaceutical preparations with satisfactory results. Furthermore, the suitable reaction conditions, affecting factors as well as the influence of coexisting substances were studied. Some possible reaction mechanisms were also discussed.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）