JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Aiyun Guo,Qixing Zhou,Yanyu Bao,Fanghan Qian,Xu Zhou
Conjugative plasmid transfer is a major contributor to the spread of antibiotic resistance genes (ARGs). However, the role of conventional fungicides on conjugative plasmid transfer has been neglected. Based on the condition that the increasing use of the combination of nano- and conventional fungicides will lead to combined contamination, the effects of a conventional fungicide prochloraz alone or in combination with nano-CuO on the conjugation of plasmid RP4 between Escherichia coli in phosphate-buffered saline were investigated in this study. The results demonstrated that 50 µg/L prochloraz alone significantly increased the conjugative transfer by 1.82 folds. The combination of 100 µg/L nano-CuO and prochloraz at 5, 50, and 500 µg/L significantly increased the conjugation by 2.56, 3.61, and 2.13 folds, respectively. The promotion of conjugative transfer of ARGs mediated by fungicides is mainly attributed to (i) the increased cell membrane permeability, (ii) the increased cell adhesion via enhancing the synthesis of polysaccharides in extracellular polymeric substances, and (iii) the up-regulation of the genes relevant to conjugation, oxidative stress, SOS response, outer membrane, polysaccharide export, intercellular adhesion, and ATP synthesis. Our findings provide evidence for the contribution of fungicides to ARGs transfer, which is significant to control the risk of ARGs dissemination.
研究领域:
微生物学
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环境科学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
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2025
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IF 3.8
Yibing Yan,Songye Li,Shanshan Wang,Yifan Peng,Kaikun Pi,Bin Sun,Liang Cheng,Bing Liu,Fangxiang Song
BACKGROUND Rice sheath blight, caused by Rhizoctonia solani ( R. solani) poses a significant threat to global rice production. Although prochloraz (PRO) is an effective fungicide, its poor photostability and low water solubility severely limit its field performance. We developed a sustainable, stimuli-responsive delivery system to enhance PRO efficacy and minimize environmental risks. RESULTS Multilayered mesoporous nanoparticles (MSN@PMO) were synthesized via a hard-template route and converted into hollow periodic mesoporous organosilicas (H-PMO) by sodium carbonate etching. PRO was encapsulated in H-PMO and sealed with pectin, yielding a dual pH/pectinase responsive formulation (Pec@PRO-H-PMO, PPHP). PPHP exhibited sustained release for 144 h under acidic and enzymatic conditions, and showed markedly improved photostability, foliar adhesion and rainfastness compared with PRO technical concentrate (PRO TC) and PRO water emulsion (PRO EW). Bioassays demonstrated that PPHP exhibited 1.8 times greater antifungal activity against R. solani compared to PRO TC. Ecotoxicological analysis revealed 11.9 times higher zebrafish median lethal concentration (LC₅₀) values than PRO TC over 80% viability of human bronchial epithelial (BEAS-2B) cells at 18.4 mg/L, suggesting improved biosafety. CONCLUSIONS The dual pH/pectinase triggered release of PPHP enhanced pesticide utilization and improved safety. This strategy presents a novel approach for developing stimuli-responsive pesticide delivery systems, aiming to strengthen crop disease control efficiency and mitigate environmental risks. © 2025 Society of Chemical Industry.
研究领域:
农业化学
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农业纳米技术
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植物病理学
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农药制剂
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控释系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PLANT PATHOLOGY
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2025
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IF 1.9
Huang Zhixing,He Zhenrui,Ding Jiexin,Zhu Yiming,Zhou Erxun
Soybean root rot, caused by Fusarium neocosmosporiellum , poses a significant threat to soybean productivity. Although chemical fungicides are widely used, their environmental risk necessitate sustainable alternatives. This study proposes an integrated control strategy combining the biocontrol bacterium Pseudomonas aeruginosa E1 - 23 with prochloraz. Initial screening of seven candidate bacterial strains identified E1 - 23 as the most potent antagonist, exhibiting 64.1% inhibition against F. neocosmosporiellum in dual-culture assays. Concurrent fungicide evaluation revealed prochloraz as the optimal agent (EC 50 = 0.1646 μg/mL). Compatibility analysis demonstrated E1 - 23's robust tolerance to prochloraz concentrations at ≤ 50 μg/mL. In vitro combination of prochloraz (0.25 μg/mL) and E1 - 23 (1 × 10 6 CFU/mL) achieved 91.86% pathogen suppression, surpassing individual treatments by > 10% and theoretical additive effects. Greenhouse trials confirmed enhanced efficacy, with integrated application increasing disease control efficacy by 34.35%—significantly outperforming standalone bacterial (18.96%) or fungicide (20.52%) controls. The qRT-PCR analysis revealed that the combination application of E1 - 23 and prochloraz up-regulated the expression of some defense genes of soybean plants 5 days post-inoculation (dpi), but the induced plant disease resistance was low. This study highlights the potential of E1 - 23-prochloraz as an eco-friendly approach to mitigate soybean root rot while reducing fungicide dependency.
研究领域:
植物病理学
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农业科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2025
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IF 3.2
Luo Yuwei,Zhang Yonghua,Chen Xiumei,Che Jinxin,Tao Nengguo
AimsTo overcome the hydrophobicity and effumability restriction of the essential oil in practice, we aim to fabricate the (E)-2-octenal-β-cyclodextrin inclusion complex (O/β-CDIC) and investigate its encapsulation mechanism.Methods and ResultsThe O/β-CDIC was fabricated using saturated aqueous solution method combined with freeze-dried method. The encapsulation efficiency and loading content of O/β-CDIC were 45.61 ± 1.37% and 2.97 ± 0.08%. In vitro assays, the MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of the O/β-CDIC were 2.00 and 8.00 mg·mL⁻¹ for P. italicum XX5 mycelial growth, respectively. In addition, the O/β-CDIC effectively reduced the incidence of blue mold in citrus fruit and maintained fruit quality. The detailed structure characterization combined with the molecular docking study, molecular electrostatic potential, and independent gradient model based on Hirshfeld analysis collectively demonstrated that (E)-2-octenal was entrapped successfully within the β-CD cavity.ConclusionsThe O/β-CDIC significantly enhanced the thermal stability and phase solubility of (E)-2-octenal while effectively preserving its antifungal activity. Characterization analyses confirmed the presence of non-covalent interactions between β-CD and (E)-2-octenal.
研究领域:
食品科学
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植物病理学
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化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）