FOOD CONTROL
·
2023
·
IF 6
Yanhua Xu,Huiyan Dong,Chenxi Liu,Haiwei Lou,Renyong Zhao
Aflatoxin B 1 (AFB 1 ) is the most common mycotoxin with the highest toxicity, posing a threat for food contamination. In this study, the bacterium Pseudomonas aeruginosa M-4 was isolated from rotten wood, and its culture supernatant showed a 56.79% AFB 1 degradation rate. Treatment with proteinase K, sodium dodecyl sulfate, and trichloroacetic acid decreased the AFB 1 degradation rate in the culture supernatant to 20.71%, 10.83%, and 19.62%, respectively. Moreover, heating the culture supernatant at 121 °C for 30 min increased in the AFB 1 degradation rate to 84.76%, indicating effective degradation by heat-resistant and inducible extracellular proteins or enzymes. P. aeruginosa M-4 reduced the content of AFB 1 in contaminated maize (90.57%, 14 d). Additionally, high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry analyses showed that AFB 1 was biotransformed into structurally different compounds, (C 17 H 16 O 6 ), (C 16 H 14 O 5 ), (C 17 H 14 O 5 ), and (C 16 H 10 O 6 ). According to the structure-activity relationship, these degradation products were produced by the destruction of partial toxic sites of AFB 1 , so the toxicity of these products may be lower than that of AFB 1 . Thus, P . aeruginosa M-4 has excellent potential for application in the detoxification of AFB 1 in food and feed.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Junpeng Li,Rui Bai,Wei Chen,Chongyuan Ren,Fan Yang,Xiaochun Tian,Xiaofeng Xiao,Feng Zhao
Mineralization of lead ions (Pb 2+ ) to pyromorphite using phosphorus-containing materials is an effective way to remediate lead (Pb) contamination. Bone char is rich in phosphorus, but its immobilization of Pb 2+ is limited by poor phosphate release. To utilize the phosphorus in bone char and provide a suitable growth environment for phosphate-solubilizing bacteria, bone char and Pseudomonas rhodesiae HP-7 were encapsulated into bio-beads, and the immobilization performance and mechanism of Pb in solution and soil by bio-beads were investigated. The results showed that 137 mg/g of phosphorus was released from bone char in the presence of the HP-7 strain. Pb 2+ removal efficiency reached 100 % with an initial Pb 2+ concentration of 1 mM, bone char content of 6 g/L, and bio-bead dosage of 1 %. Most Pb 2+ was immobilized on the surface of the bio-beads as Pb 5 (PO 4 ) 3 Cl. The soil remediation experiments showed a 34 % reduction in the acid-soluble fraction of Pb. The bio-beads showed good stability in long-term (30 d) soil remediation. The present study shows that bone char can be turned into an efficient Pb immobilization material in the presence of phosphate-solubilizing bacteria. Thus, bio-beads are expected to be used in the remediation of Pb-contaminated environments.
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ENVIRONMENTAL POLLUTION
·
2020
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IF 6.793
Yihao Li,Huimin Wang,Pingxiao Wu,Langfeng Yu,Saeed Rehman,Junfeng Wang,Shanshan Yang,Nengwu Zhu
The effective mineral absorption and bioreduction were considered as two preferred processes to alleviate the bioavailability and toxicity of toxic trace metals. In this study, the bioreduction of hexavalent chromium (Cr(VI)) on goethite (FeOOH) in the presence of Pseudomonas aeruginosa ( P. aeruginosa ) was investigated with different environmental factors, including carbon source concentrations, pH, temperature and initial Cr(VI) concentrations. The characterization of FeOOH– P. aeruginosa indicated that P. aeruginosa was surrounded by FeOOH, which could provide the essential iron for bacterial growth and reduce Cr(VI) to Cr(III). The optimal experimental conditions for Cr(VI) (initial concentration: 35 mg L −1 ) absorption (∼46%) and bioreduction (∼54%) involved a temperature of 45 °C and pH of 5.5. Meanwhile, extracellular polymeric substances (EPS) secreted by P. aeruginosa and its functional groups played important roles in the reduction of Cr(VI). They could reduce Cr(VI) to Cr(III) and transform to Cr(OH) 3 or Fe x -Cr (1-x) (OH) 3 precipitation. These results of this study are of significant importance to better understand the environmental geochemical behavior of Cr(VI) with the interactions between soil minerals and microorganisms .
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Scientific African
·
2020
·
IF 0
Sujan Khadka,Jean Bosco Nshimiyimana,Piao Zou,Niranjan Koirala,Li Xiong
Environmental pollution due to di-ethyl phthalate (DEP) is one of the major concerns all over the world. This is probably the first study regarding the biodegradation of DEP by newly isolated Pseudomonas juntendi strain CCNU-SK1, P. putida strain CCNU-SK2 and P. nitritireducens strain CCNU-SK3. Bacterial strains were isolated by morphological, biochemical, physiological and 16S rDNA gene sequencing techniques. The biodegradation of DEP was quantified, and degradation kinetics were studied by using high-performance liquid chromatography. The optimum growth of bacteria for strain CCNU-SK1 was obtained at 28 °C, pH 8, DEP (500 mg/L), NaCl (1.5%), half-life (10.19 h); in case of strain CCNU-SK2: 33 °C, pH 7, DEP (400 mg/L), NaCl (1.5%), half-life (13.86 h) and for strain CCNU-SK3: 33 °C, pH 7, DEP (500 mg/L), NaCl (1.75%), half-life (12.6 h). Strain CCNU-SK1 with lowest half-life period was the most efficient bacteria for biodegradation of DEP compared to other isolated strains. More than 99% of all DEP were biodegraded within the 4 days and the complete utilization of (50 mg/L) DEP occurred within 24 h by all strains. All of them were capable of metabolizing not only DEP but also other phthalate esters (PAEs) such as DMP (Di-methyl phthalate), DBP (Di-butyl phthalate), DOP (Di-octyl phthalate), BBP (Benzyl butyl phthalate), DEHP (Di-2-ethylhexyl phthalate) and PA (Phthalic acid), making them the promising agents for the bioremediation in the PAEs contaminated sites.
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Water
·
2023
·Vol. 15
·期 4
·830
·
IF 3.4
Shuaiguo Li,Kejian Tian,Qing Qiu,Yue Yu,Han Li,Menghan Chang,Xuejian Sun,Jinming Gu,Fenglin Zhang,Yibing Wang,Hongliang Huo
As a widespread pollutant, bisphenol A (BPA) has created a serious threat to ecosystem and human health. Therefore, expanding the available microbial resources used to screen highly efficient BPA-degrading bacteria with BPA as the sole carbon source is very important for the removal of this pollutant from the environment. In this study, the BPA degradation rate ofPseudomonassp. P1 to 30 mg/L was 96.89% within 120 h. Whole genome sequencing showed that the genome of strain P1 was composed of a single circular chromosome with a full length of 6.17 Mb, which contained 5636 predicted coding genes. Comparative genomic analysis showed that strain P1 contained 210 functional genes related to BPA degradation. It was confirmed that BPA degradation genes ferredoxin (bisdA), P450 (bisdB),CotAandLacin strain P1 were highly expressed under the induction of BPA. Combined with the identification of metabolites, the route of BPA degradation byPseudomonaswas proposed. A new metabolite, 4-vinylphenol, was detected for the first time in pathway Ⅰ. In pathway Ⅱ, BPA is directly oxidized to phenol and 4-isopropenyl phenol in the presence of laccase, which is rarely reported in the process of bacterial degradation of BPA. This study confirmed that strain P1 had good tolerance to various environmental factors at the gene level and enriched the degradation mechanism of BPA.Keywords:Bisphenol A;Pseudomonas;whole genome;biodegradation
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ANALYTICAL CHEMISTRY
·
2018
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IF 6.042
Yong He,Yanli Shi,Mengli Liu,Yingran Wang,Lin Wang,Shuguang Lu,Zhifeng Fu
Rapid and accurate bacterial detection is crucial to an early diagnosis for treating various infectious diseases. A recombinant tail fiber protein (P069) of the Pseudomonas aeruginosa (P. aeruginosa) phage was expressed in Escherichia coli. After renaturation at a low temperature, the inclusion body of P069 was successfully transformed to an aqueous soluble protein that retained the capacity for recognizing P. aeruginosa. The recombinant P069 did not show lytic activity to P. aeruginosa, which facilitated the capture and manipulation of bacterial whole cells with a high flexibility for downstream identification and detection. Bioluminescent and fluorescent methods using this biorecognition element allowed P. aeruginosa detection with the detection limits of 6.7 × 102 CFU mL–1 and 1.7 × 102 CFU mL–1, respectively. Moreover, the specificity investigations showed that P069 was a species-specific protein. Therefore, it avoided the potential false negative results originating from the excessive high specificity of phage toward a given strain. It has been successfully applied to detect P. aeruginosa in spiked samples with acceptable recovery values ranging from 88% to 98%. The above results demonstrate that P069 is an ideal biorecognition element for the detection of P. aeruginosa in complicated sample matrixes.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 11
·1-7
·
IF 5.705
Wu Zhengzong,He Deyun,Cui Bo,Jin Zhengyu
An aptamer based assay is described for the determination of Pseudomonas aeruginosa ( P. aeruginosa ). It is bimodal in that both surface enhanced Raman spectroscopy (SERS) and colorimetry are applied for quantitation. The aptamer against P. aeruginosa and its corresponding complementary DNA fragment (cDNA) were conjugated to two kinds of differently sized gold nanoparticles (AuNPs). The 30 nm AuNPs carrying the aptamer are used as color signal probes, while the cDNA-15 nm AuNP conjugates serve as SERS signalling probes. In the absence of P. aeruginosa , the two probes assemble to form the duplex structure. When the probes are exposed to P. aeruginosa , the aptamer dissociates from its cDNA and binds the target. After centrifugation, the SERS signal from the supernatant decreased, due to the decreased electromagnetic effect. On addition of 3,3′,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide, a relatively rapid catalytic reaction between horseradish peroxidase (linked to the aptamer on the 30-nm AuNPs) and TMB occurred, resulting in the formation of a green color with an absorption peak at 640 nm. The reliability of the approach was validated by detecting different levels of P. aeruginosa in spiked tap water and chicken meat samples. The average recoveries ranged from 88% to 112%, confirming the practicality of this method. In our perception, this dual mode aptasensor paves the way for accurate and reliable determination of P. aeruginosa . Conceivably, the method has a wide scope in that it may be extended to the determination of various other species for which respective aptamers are available.
Schematic illustration of a bimodal aptasensor based on surface enhanced Raman scattering (SERS) and color change for the detection of Pseudomonas aeruginosa .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Govindan Rajivgandhi,Muthuchamy Maruthupandy,Thangasamy Veeramani,Franck Quero,Wen-Jun Li
In the present investigation functional chitosan/silver nanocomposites (CS/Ag NCs) were successfully synthesized and found to possess favorable antibacterial activity against extended spectrum beta-lactasame (ESBL) producing Pseudomonas aeruginosa . Powder X-ray diffraction showed that the obtained CS/Ag NCs are constituted of highly crystalline Ag nanoparticles (NPs) embedded in an amorphous CS matrix material. Transmission electron microscopy (TEM) analysis provided structural information about CS/Ag NCs, revealing the formation of spherical cluster structures constituted of Ag NPs with size ranging from 6 to 18 nm embedded in the amorphous CS matrix. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Ag NPs and CS/Ag NCs were found to inhibit the ESBL producing P. aeruginosa at 80 μg/mL (76%) and 50 μg/mL (92%), respectively. Confocal laser scanning microscope (CLSM) and scanning electron microscopy (SEM) images revealed that P. aeruginosa experienced reduced cell viability and morphological cell membrane damage at desired MIC. The in-vivo toxicity effect of Ag NPs and CS/Ag NCs suggested an increased mortality rate when Artemia franciscana were exposed for 24 h to increasing concentrations of Ag NPs and CS/Ag NCs. Anti-ESBL activity and toxicity effect of CS/Ag NCs revealed that these NCs possess promising antibacterial properties to overcome numerous communicable bacterial strains .
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BIOCONJUGATE CHEMISTRY
·
2018
·
IF 4.485
Yu Zhao,Zhentan Lu,Xiaomei Dai,Xiaosong Wei,Yunjian Yu,Xuelei Chen,Xinge Zhang,Chaoxing Li
Due to the rapid development of bacterial resistance, there is an urgent need to explore new antibacterial agents to substitute for traditional antibiotic therapy. Photodynamic therapy has been identified as a promising bactericidal method to conquer antibiotic-resistant pathogens. To solve the problem of photosensitizer damage to normal tissues in vivo, we developed a boron-dipyrrolemethene (BODIPY)-based glycosylated photosensitizer for ablating Pseudomonas aeruginosa (P. aeruginosa). This glycosylated photosensitizer exhibited good water solubility and generated 1O2 rapidly in an aqueous solution under light exposure. The photosensitizer did not cause detectable toxicity to human cells in the dark. Importantly, the photosensitizer was able to selectively attach to P. aeruginosa over normal cells, thus resulting in effective pathogen ablation by reactive oxygen species. Moreover, the photosensitizer inhibited over 90% of the biofilm formation produced by P. aeruginosa. The results indicate that the design of the macromolecular photosensitizer-induced bacterial death and inhibited biofilm formation provide a novel strategy for overcoming bacterial infection.
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Journal of Cleaner Production
·
2024
·
IF 9.7
Chun-dan Gan,Jin-yan Yang,Jiang-fei Li,Meng-qi Yang,Xin-yue Du,Aleksander Nikitin
The continuously elevated concentration of vanadium (V) in the environment is of great concern due to its potential risks to organisms. Microbial reduction is considered a green way to remediate the V-contaminated sites. This study presents the first demonstration of Pseudomonas balearica , a bacterium flourishing in vanadium-titanium magnetite tailings, exhibiting the capacity to reduce pentavalent V [V(V)]. We found that P. balearica exhibited strong tolerance to high V(V) levels, despite lacking known V(V)-reducing genes. After 48 h of incubation, the reduction rates of V(V) by P. balearica under 0.5 mM and 10 mM V(V) stress reached 81.1% and 60.6%, respectively. The phosphorus group and carboxyl group on the bacterial cell surface contributed to V(V) binding and reduction. Both extracellular and intracellular V(V) reduction was significantly facilitated by many critical V-induced differentially expressed genes (DEGs) encoding nitrate/nitrite reductases, ABC transporters , NADH, and c-type cytochromes . In addition, up-regulation of DEGs associated with reactive oxygen species (ROS)-scavenging enzymes , such as glutathione peroxidase, superoxide dismutase , and catalase , was observed as a protective response to oxidative stress triggered by V(V). Moreover, some up-regulated DEGs ( ureC , ureE , ureA , and ureG ) encoding urease subunit or urease accessory proteins significantly enriched in the nickel cation binding pathway, indicating that P. balearica may be resistant to Ni stress by regulating urease activity. This study will help to more thoroughly elucidate the characteristics and mechanisms of V(V) reduction by microbes and provide a native bacterium flourishing in tailings for potential bioremediation of V pollution and bioleaching of V-sources.
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Food Bioscience
·
2025
·
IF 5.9
Hongwei Zhan,Xiang Zou,Jiao Li,Lixiu Zhang,Guanyuan Qu,Yanqing Li,Yongjun Sun,Wenming Ju,Mengqi Ye,Yang Deng,Ting Ding
Pseudomonas fluorescens is a prevalent spoilage bacterium in various chilled aquatic products, producing toxic biogenic amines (BAs) through the decarboxylation of amino acids via amino acid decarboxylases. Our previous studies revealed that P. fluorescens PF12 regulates BA production through its quorum sensing (QS) system, suggesting that disrupting QS could be a potential approach to control BA generation. In this study, we targeted the three-dimensional structure of the LuxI-type protein in P. fluorescens PF12 to screen for QS inhibitors (QSIs) through high-throughput screening from Seaweed Metabolite Database and Comprehensive Marine Natural Products Database. Nine compounds with high binding scores to the LuxI-type protein were identified. Considering cost-effectiveness and safety, phytol was selected for further investigation. Experimental results demonstrated that sub-inhibitory concentrations of phytol significantly suppressed BA production, siderophore production, motility, extracellular protease secretion, and biofilm formation in P. fluorescens PF12. Specifically, when the salmon fillets were stored for 288 h, the histamine content in the fillets treated with 25 mg/mL phytol decreased by 43.81 %. RT-qPCR revealed that the expression levels of key genes hisD and hisC related to histamine synthesis in the phytol treatment group were reduced by 7.68 ( p < 0.05) and 8.23 % ( p < 0.05), respectively, compared to the control group. Molecular docking analysis revealed that phytol interacts with key residues in the LuxI-type protein, thereby blocking the synthesis of acyl-homoserine lactones (AHLs) and downstream QS signaling. These findings indicate that phytol has great potential for controlling BA production in aquatic products and ensuring food safety.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-19
·
IF 12.6
Du Chunhong,Wang Shuai,Cheng Yijie,Li Jie,Zhang Yufei,Li Zhuohao,Zhu Baolin,Wu Zhongming,Zhang Xinge,Zhou Lingyi
Pneumonia caused by Pseudomonas aeruginosa ( P. aeruginosa ) infection remains a formidable clinical challenge due to persistent biofilm formation and intrinsic antibiotic resistance, exacerbated by bacterial iron homeostasis that stabilizes biofilm architecture and neutralizes oxidative stress. Herein, we present Fe/TNT@NM, a biomimetic nanosonosensitizer activated by ultrasound (US) to dismantle biofilms through dual extracellular-intracellular mechanisms. The nanosonosensitizer features an iron-doped titanate nanotube (Fe/TNT) core encapsulated within a neutrophil membrane (NM). Under US irradiation, Fe/TNT@NM generates sonodynamic reactive oxygen species (ROS) extracellularly and enhances Fe 3+ release. These ions catalyze the Fenton reaction extracellularly to amplify chemodynamic effects and disrupt intracellular iron homeostasis, triggering bacterial ferroptosis. The NM coating enables immune evasion and biofilm-targeted delivery. This ultrasound-reinforced ferroptosis strategy synchronizes extracellular ROS storms with intracellular iron dyshomeostasis, achieving dual-action biofilm dismantling and eradication of drug-resistant P. aeruginosa . In a murine pneumonia model, Fe/TNT@NM suppresses biofilms and mitigates pulmonary injury. By converging biomimetic targeting, sonodynamic-chemodynamic cascades, and ultrasound-augmented ferroptosis, this nanosonosensitizer presents a paradigm-shifting approach to combat refractory biofilm infections and antibiotic resistance. Graphical
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